• 한국토양비료학회지
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• 제49권5호
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• pp.577-583
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• 2016

Small-sized persimmons produced by high crop load are better accepted in the export markets. However, maintaining high crop load frequently results in weakness of tree vigor, deterioration of fruit quality, and increase of the risks for alternate bearing. This experiment was conducted to determine the combined effects of fertilization rate and leaf-fruit (L/F) ratio on container-grown 3-year-old 'Fuyu' persimmon trees. Application of 3.6-g N, 2.1-g $P_2O_5$, 2.7-g $K_2O$, 2.7-g CaO, and 0.6-g MgO was for the control fertilization rate (CF) and that of a 3-fold CF was for the high fertilization rate (HF). Commercial fertilizers were surface-applied to a container on July 6, July 17, and August 10 in three equal aliquots. Single tree for each fertilization rate was assigned for 12 L/F ratios (5, 6.3, 7.7, 9, 10.4, 13, 15.5, 18, 21, 24, 27, and 33) mostly by fruit thinning or rarely by defoliation on July 1. HF did not affect the yield, weight and soluble solids of the fruits but decreased skin color. As L/F ratio increased, yield decreased but average weight, skin color, and soluble solids of fruits increased. With HF, N and K concentrations in leaves, fruits, and shoots increased to some extent but soluble sugars in dormant shoots decreased. Many shoots were cold-injured with low L/F ratio especially at the HF. HF did not increase number of flower buds the next spring either on a shoot or on a tree basis but increased shoot length, compared with the CF. Increasing L/F ratio markedly increased number of flower buds and shoot growth the following year at both fertilization rates. Therefore, an appropriate combination of fertilization rate and L/F ratio should be necessary to maintain stable fruit production and tree vigor at high crop load.

• Clinical and Experimental Reproductive Medicine
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• 제19권2호
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• pp.163-168
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• 1992

This study was carried out to set up the ovum bank for ovum donation and to determine the best freezing method for human immature oocytes. Human immature follicular oocytes were cryopreserved by slow freezing and rapid thawing method. Immature follicular oocytes were treated by propanediol(PROH) solution by 2 and 4 step method in protocols A & B, respectively. In protocol C, immature oocytes were exposed to sucrose prior to treatment of PROH by 4 step method. We compared survival rate, maturation rate, and fertilization rate of immature oocytes among three protocols. Results were as follows. 1. Oocytes treated by the protocol C showed the highest survival rate( 70.3 %) and maturation rate(34.6%) after thawing. 2. Survival rate of oocytes treated by the protocol C was significantly higher than that of the protocol B after thawing(p<0.05). In conclusion, treatment of oocytes with sucrose prior to expose PROH was the best freezing method. Sucrose may have reduced the toxic effect of cryoprotectant to oocytes. We failed to induce fertilization of oocytes, which were treated by any protocols, by conventional insemination method, but obtained 28.8% fertilization rate by using partial zona dissection(PZD) method. This result suggests that micromanipulation(PZD) of the thawed oocytes before insemination will improve the fertilization rate.

• Clinical and Experimental Reproductive Medicine
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• 제21권3호
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• pp.247-252
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• 1994

Intracytoplasmic sperm injection(ICSI) was known as effective method in treatments of couples who unable to be helped by conventional in vitro fertilization. In 78 treatment cycles of 78 infertile couples using ICSI performed at our infertility clinic between May and August 1994 were analyzed. These patients were classified two groups, andrological factor(AF) and non-andrological factor(non-AF) group. The AF group, which had abnormal sperm physiology, included oligozoospermia, asthenozoospermia, oligoasthenoteratozoospermia(OATS) and microsurgical epididymal sperm aspiration(MESA) patients. The non-AF group, which had abnormal oocyte physiology, included abnormal zona pellucida, poor quality of oocyte and immune factor infertile patients. A single spermatozoon was injected into the ooplasm of 776 metaphase II oocytes. The fertilization rate was 44.6%(346/776) and 319 embryos were transferred. After 73 embryo transfers(93.6% of treatment cycles) 23 pregnancies were estabilshed, i. e. pregnancy rate of 29.4% per started cycle and 31.5% per embryo transfer. Fertilization rate of AF and non-AF group was 46.2% and 35.8%, pregnancy rate was 34.5%(20/58) and 20.0%(3/15), respectively. In order to increase the pregnancy rate, assisted hatching(AHA) has done after lCSl in 47 treatment cycles. Pregnancy rate of ICSI with AHA and without AHA group was 34. 0% (16/47) and 26.9%(7/26), respectively. ICSI was more effective in andrological factor infertility and the pregnancy rate was increased by ICSI with AHA procedure.

• Clinical and Experimental Reproductive Medicine
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• 제21권1호
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• pp.21-29
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• 1994

Subzonal insemination(SUZI) has been proposed for patients with severe male factor and previous fertilization failure. However, very low fertilization rates still persisted. The aims of this study were firstly, to examine the relationships between the fertilization rate and sperm parmeters, sperm incubation media and time, secondly, to evaluate the outcome of 119 cycles of SUZI applied the modified sperm preparation method. The fertilization rates were influenced more sensitively by sperm preincubation media and time than by sperm parameters. According to preincubation media and time, the fertilization rates were 43.3% in 50% follicular fluid (HFF), 36.6% in 10% fetal cord serum(FCS), and with the time, increased in FCS, but decreased in HFF. In regrd with sperm parameters, the fertilization rates were 42.9% in normal and 37.6% in subnormal group. The best results were obtained from SUZI by the spermatozoa incubated in 50% HFF for 6-8 hours. So we tried 119 cycles of SUZI(normal; 39 cycles, subnormal; 80 cycles) using the preparation method of 6-8 hour incubation in 50% HFF. There were no signigicant differences in the fertilization rates between normal(125/269, 46.4%) and subnormal sperm(264/635, 41.6%). Contrary to the fertilization rates, pregnancy outcomes were different between both groups. Better results obtained from the subnormal group than the normal in the number of transferred embryos, that of good embryos, and developmental rate of the fertilized eggs. The pregnancy rates per transfer were totally 13.3%(13/98),20.0%(13/65) in subnormal group. In the normal group, 2 patients showed ${\beta}$-hCG positive, but resulted in chemical pregnancy. Of 13 clinical pregnancies, two aborted, 6 on-going, and 5 delivered. In conclusion, SUZI is an effective technique to overcome fertilization failure for male factor and unexplained. The fertilization rate is influenced by sperm parameters, sperm incubation media and time. Also the quality of oocytes might be important for pregnancy as same as that of sperm.

• 한국토양비료학회지
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• 제39권5호
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• pp.298-302
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• 2006

비닐피복작물 재배시 시비효율 증대 및 생력시비기술 확립을 위하여 파종과 동시에 시비가 가능한 토중시비기를 개발하였고 개발된 파종기와 시비기를 이용하여 1999년부터 2000년까지 시비간격 30 cm, 시비깊이 15 cm, 파종간격 25 cm로 배수가 양호한 사양토에서 땅콩을 재배한 후 작업정도, 토양화학성, 양분흡수량 및 수량성에 미치는 영향을 평가하였다. 땅콩의 파종 소요시간은 인력($173hr\;ha^{-1}$)에 비하여 90% 이상 노력절감 효과가 있었으나 출현일수는 4일정도 지연되었으며 입모율은 관행구에 비하여 11~18% 낮았다. 시험 전 토양에 비하여 시험 후 토양의 총질소 함량은 증가한 반면에 유기물, 유효인산 및 치환성 칼륨함량은 감소되었으며 토양의 질산태질소 합량은 생육초기에서 중기까지 국소시비구에서 시비량이 많을수록 증가하였다. 땅콩의 질소흡수량은 근류균에 의한 자체 고정량이 많아 시비방법 및 시비량별 큰 차이가 없었으나 인산 및 칼리흡수량은 국소시비 70%, 100%구에서 높은 경향을 나타냈다. 땅콩의 생장속도는 시비방법별 공히 파종 후 120일에 가장 많이 증가되었고 관행시비구와 국소시비 70%구에서 생육후기까지 증가폭이 크게 나타났으며 국소시비 70%구는 협수가 많고 협실비율 및 완숙립중 비율이 높아 종실중이 관행시비구 $3,150kg\;ha^{-1}$에 비하여 4% 증수되었다.

• Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.417-420
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• 2011

In this experiment, we examined the survival, fertilization, hatching times, and hatching rates of Far Eastern catfish Silurus asotus at pH ranging from 2 to 13 under laboratory conditions. Eggs could be fertilized at pH 3-12. In a hatching experiment, mortality was first observed at pH 13, when all fertilized eggs died within 8 min, followed by pH 2 (30 min), pH 12 (60 min), pH 3 (4 h), and pH 11 (5 h). Hatching only occurred at pH 4-10, with the highest hatching rate at pH 7 (52%) and the lowest at pH 10 (24%). Hatching rates in acid solutions were higher than in alkaline solutions, although the difference was not significant. Hatching was first observed at pH 10, beginning 27 h after fertilization and ending at the 31 h. A clear difference was observed between hatching times, ranging from 31 to 64 h and increasing in order with descending pH.

• 한국가축번식학회지
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• 제16권2호
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• pp.157-164
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• 1992

This stduy was carried out to obtain basic information available on ovulation, spawning, fertilization rate, hatching rate, and deformity rate after hCG injection in cyprinid loach, Misgurnus mizolepis. The results obtained in these experiments were as follows: 1. Ovulation and spawning occurred simultaneously and spawing was completed within 1 hour after ovulation. 2. More than 80% of fertilization rates appeared within 12 hours at 21$^{\circ}C$, 8 hours at $25^{\circ}C$, and 4 hours at 29$^{\circ}C$, respectively, following the onset of spawing. Afterwards, the fertilization rates of released eggs sharply decreased in three different water temperatures. 3. More than 70% of hatching rates appeared within 8 hours at 21$^{\circ}C$, 6 hours at $25^{\circ}C$, and 2 hours at 29$^{\circ}C$, respectively, following the onset of spawing. Afterwards, hatching rates of spawned eggs abruptly decreased in three different water temperatures. 4. The deformity rates of hatched larvae were high at $25^{\circ}C$, 8 hours following the onset of spawing. 5. Based on the developmental ability of oocytes, the optimum time of fertilization was 4 hours (stage 5) following the onset of spawing.

• Clinical and Experimental Reproductive Medicine
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• 제11권2호
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• pp.59-67
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• 1984

In vitro fertilization have been performed to know whether the frozen semen has fertilizing ability and can be used clinically. The results of cultured and developed embryos obtained are as follows: 1. The semen was frozen in three media for the good viability. The viability was more than 50% and the motility was also moderate (grade III), 2. As the 33 oocytes were collected from 45 follicles, the oocyte recovery rate was 73.3%. Among them, mature and immature ova were 5% each, and premature ova were 69.7%, When the first polar body was appeared, above ova were inseminated after adequate incubation with activated sperms. 3. The main components of three freezing medium containing egg yolk, glycerol and pyruvate respectively were the best for sperm viability, and Ham's F-10 medium was used for the fertilization and culture of eggs. 4. The results of in vitro fertilization of 33 ova, showed the second polar body developed in 12%, polyspermia in 24%, 1-cell embryo in 21% and 2-cell embryo in 9%. One mature ova developed to blastocyst via 16-cell to 32-cell embryo. The fertilization rate was 66%. 5. Above mentioned results represent that the frozen semen has fertilizing ability and can be used practically in the clinic.

• 한국초지조사료학회지
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• 제8권1호
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• pp.40-46
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• 1988

This study was carried out to determine the effects of nitrogen (N) fertilization levels (0, 100 and 200kg/ha) and formaldehyde (0.4,0.8 and 1.2%: w/w CM) on the chemical composition, dry matter (DM) yield and in-vitro dry matter digestibility (IVDMD) of forage rape (Brassica napus Subsp. oleifera) silage. The results obtained are summarized as follows : 1. Forage rape was a catch crop which was highly digestible and low concentration in NDF, ADF, cellulose and lignin. 2. The concentration of the water soluble carbohydrate (WSC) and buffering capacity (PK) was 17.9% and 6.77g/100g, respectively, provided the WSC to PK ratio was 2.65. 3. DM content of silage decreased as the rate of N fertilization increased, but concentration of ammonia-N decreased. IVDMD was not affected by the rate of N fertilization. 4. By the addition of formaldehyde, the pH and IVDMD were increased, but the DM, lactic acid, total organic acid and ammonia-N content of rape silage were decreased, that was effective as a silage additive for forage rape.

• 한국산림과학회지
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• 제104권1호
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• pp.90-97
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• 2015

본 연구는 주요 활엽수 조림수종인 느티나무, 참느릅나무, 거제수나무 및 피나무를 대상으로 세 가지 시비 처리($0.5g{\cdot}L^{-1}$, $1.0g{\cdot}L^{-1}$, $2.0g{\cdot}L^{-1}$)에 따른 근원경, 간장, 물질생산량, 묘목품질지수 등 생장 특성을 조사 분석하여 시설양묘과정에서 실질적으로 적용 가능한 수종별 적정 시비기술을 구명하고자 수행하였다. 느티나무는 $2.0g{\cdot}L^{-1}$, 거제수나무는 $0.5g{\cdot}L^{-1}$ 시비처리구에서 가장 우수한 생장 및 묘목품질을 나타냈는데, 시비수준에 따른 두 수종간의 생장 특성이 반대의 경향을 보인 것이다. 또한, 시비처리에 따른 참느릅나무와 피나무의 근원경과 간장 생장은 유의적 차이가 없었다. 그러나 묘목품질지수는 두 수종 모두 $0.5g{\cdot}L^{-1}$ 시비처리구에서 가장 낮았으며, $1.0g{\cdot}L^{-1}$과 $2.0g{\cdot}L^{-1}$ 시비처리구 간 유의적 차이는 없었다. 따라서 묘목 품질과 함께 경제적, 환경적 측면을 고려했을 때 느티나무는 최소 $2.0g{\cdot}L^{-1}$, 참느릅나무와 피나무는 $1.0g{\cdot}L^{-1}$, 거제수나무는 최대 $0.5g{\cdot}L^{-1}$이 적정 시비 수준으로 판단된다. 수종별 적정 시비 수준(배액, 회수)의 적용으로 우량 묘목 생산 및 이와 연계된 조림성과 향상뿐만 아니라 환경오염 저감, 생산비용 절감 및 양묘 기간 단축 등 경제적 이점이 기대된다.