• Journal of Microbiology and Biotechnology
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• 제34권6호
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• pp.1189-1196
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• 2024

Bacterial resistance to commonly used antibiotics is one of the major challenges to be solved today. Bacteriophage endolysins (Lysins) have become a hot research topic as a new class of antibacterial agents. They have promising applications in bacterial infection prevention and control in multiple fields, such as livestock and poultry farming, food safety, clinical medicine and pathogen detection. However, many phage endolysins display low bactericidal activities, short half-life and narrow lytic spectrums. Therefore, some methods have been used to improve the enzyme properties (bactericidal activity, lysis spectrum, stability and targeting the substrate, etc) of bacteriophage endolysins, including deletion or addition of domains, DNA mutagenesis, chimerization of domains, fusion to the membrane-penetrating peptides, fusion with domains targeting outer membrane transport systems, encapsulation, the usage of outer membrane permeabilizers. In this review, research progress on the strategies for improving their enzyme properties are systematically presented, with a view to provide references for the development of lysins with excellent performances.

• 한국유기농업학회지
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• 제25권4호
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• pp.821-830
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• 2017

본 연구는 10종 허브 추출물에 대한 DPPH 자유라디칼 소거능, 항균효과 및 세포독성에 미치는 영향을 조사함으로써 항생제 대체제로서 활용 가능성을 위한 기초 자료로 제시하고자 수행하였다. 10종의 허브 추출물의 DPPH 자유라디칼 소거능은 로즈마리, 오레가노 및 캐모마일이 각 49.9, 48.9, 47.7%으로 유의적으로 가장 높았다. 허브 추출물의 E. coli K99와 S. typhimurium에 대한 항균활성은 베르가못, 오레가노, 타임과 로즈마리가 두 균주에 공통적으로 활성이 높게 측정되었으며, 활성이 나타나는 농도에서의 세포독성이 비교적 낮아 안전한 것으로 관찰되었다. 본 연구 결과 유기 축산을 위한 가축 내 천연 항생제 대체제로 항균 및 항산화 활성이 높고, 세포독성이 낮게 나타난 오레가노와 로즈마리가 천연물질로 활용 가능할 것으로 판단되며, 다양한 추출 조건으로 최적 추출 효율을 검토하여 추가적인 생리활성물질 검정과 해당 물질에 대한 연구가 필요하다.

• Journal of Radiation Protection and Research
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• 제43권2호
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• pp.39-49
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• 2018

Background: A model to assess the activity concentration of agricultural products and the public ingestion dose as result of a nuclear accident is necessarily required to manage the contaminated agricultural systems by the accident, or to estimate the effects of chronic exposure due to food ingestion at a Level 3 PSA. Materials and Methods: A dynamic compartment model, which is composed of three sub-modules, namely, an agricultural plant contamination assessment model, an animal product contamination assessment model, and an ingestion dose assessment model has been developed based on Korean farming characteristics such as the growth characteristics of rice and stockbreeding. Results and Discussion: The application study showed that the present model can predict well the characteristics of the activity concentration for agricultural products and ingestion dose depending on the deposition date. Conclusion: The present model is very useful to predict the radioactivity concentration of agricultural foodstuffs and public ingestion dose as consequence of a nuclear accident. Consequently, it is expected to be used effectively as a module for the ingestion dose calculation of the Korean agricultural contamination management system as well as the Level 3 PSA code, which is currently being developed.

• 한국식품영양과학회지
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• 제41권7호
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• pp.1002-1008
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• 2012

본 연구에서는 시판누룩에서 분리한 Rhizopus oryzae CCS01로 쌀누룩을 제조하고 이를 이용하여 곡류 막걸리 제조하였다. R. oryzae CCS01로 제조한 쌀누룩은 시판누룩보다 탄수화물 함량은 높았으나, 수분 및 회분, 조단백질, 조지방 함량은 낮았고 당화력은 약 2.2배 정도 높게 나타났다. R. oryzae CCS01로 제조된 쌀누룩으로 제조한 막걸리는 pH와 알코올 함량은 시판누룩보다 높게 나타났으나, 당도 및 산도, 젖산균수, 효모균수, 갈변도는 낮게 나타났다. 특히 쌀누룩으로 제조한 찹쌀(GUR) 막걸리의 경우 다른 막걸리들 보다 알코올 함량이 가장 높았다. 이 결과로 R. oryzae CCS01로 제조된 쌀누룩을 이용하여 새로운 곡류 막걸리 제조가 가능할 수 있을 것으로 판단되었다.

• Journal of Microbiology and Biotechnology
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• 제19권1호
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• pp.17-22
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• 2009

The mixotrophic growth with methanol plus thiosulfate was examined in nutrient-limited mixotrophic condition for Methylobacterium goesingense CBMB5 and Methylobacterium fujisawaense CBMB37. Thiosulfate oxidation increased the growth and protein yield in mixotrophic medium that contained 150mM methanol and 20mM sodium thiosulfate, at 144 h. Respirometric study revealed that thiosulfate was the most preferable reduced inorganic sulfur source, followed by sulfite and sulfur. M. goesingense CBMB5 and M. fujisawaense CBMB37 oxidized thiosulfate directly to sulfate, and intermediate products of thiosulfate oxidation such as polythionates, sulfite, and sulfur were not detected in spent medium and they did not yield positive amplification for tested soxB primers. Enzymes of thiosulfate oxidation such as rhodanese and sulfite oxidase activities were detected in cell-free extracts of M. goesingense CBMB5, and M. fujisawaense CBMB37, and thiosulfate oxidase (tetrathionate synthase) activity was not observed. It indicated that both the organisms use the "non-S4 intermediate" sulfur oxidation pathway for thiosulfate oxidation. It is concluded from this study that M. goesingense CBMB5, and M. fujisawaense CBMB37 exhibited mixotrophic metabolism in medium containing methanol plus thiosulfate and that thiosulfate oxidation and the presence of a "Paracoccus sulfur oxidation" (PSO) pathway in methylotrophic bacteria are species dependant.

• 대한인간공학회지
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• 제35권2호
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• pp.67-74
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• 2016

Objective: The aim of this study is to develop a farming shovel to reduce workload, which helps farmers lower the risk of musculoskeletal disease. Background: Most of work using farming tools including shovels requires repetitive works and awkward postures on body parts, and it could possibly cause work-related musculoskeletal disorders. It is necessary to develop and distribute farm equipment and tools in order to reduce physical workload. Method: To improve the most uncomfortable task perceived by ten farmers during the work with a shovel, the improved shovel was designed and made as a prototype for experiment for the comparison of the existing and improved shovels. Twenty males were recruited for this experiment, and muscle activity (%MVC) of six body parts and subjective discomfort ratings by body parts while working with a shovel were measured. A paired t-test was performed to compare physical workload between the existing shovel and the developed one. Results: A shovel applying foothold tied between shaft and blade was designed, which can help workers reduce repetitive bending of back and pressures for upper limbs while digging soil. According to compared evaluation of the developed shovel and the existing shovel, the developed shovel's %MVCs in all experimental muscles were significantly lower than those of the existing shovel. The developed shovel showed the biggest drop in perceived subjective discomfort rating of back, followed by arm and neck, compared to the existing shovel. Conclusion: It was confirmed that attaching a foothold to a shovel was an effective way of reducing workload in back and upper limbs during digging. Application: In the near future, if we put the prototype of developed shovel to practical use after making up for defects, it will help farm work environment be healthier and safer.

• 한국지역지리학회지
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• 제12권3호
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• pp.339-351
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• 2006

뻘중심의 갯벌이 넓게 형성된 가로림만의 웅도리외 모래 갯벌과 염전이 발달한 영광 갯벌의 두우리, 수산 양식업이 발달한 곰소만 주변 지역을 대상으로 조석 주기, 갯벌 지형. 갯벌 퇴적물의 입도 특성과 주민 생활과의 관련성을 검토했다. 갯벌 주변 주민들에게 갯일은 농사일보다 우선되고, 갯벌에서 얻어지는 수입이 이들의 주소득원이다. 갯벌주변의 주민 생활은 조석 주기에 맞추어 이루어지고 있으며, 한사리 저조위 때는 가장 바쁘고 많은 소득을 올릴 수 있어 마치 장날과 같다. 뻘 갯벌에서는 패류 양식이 주이고, 모래 갯벌에는 안강망 어업이 활발하며, 해안에 접한 혼합 갯벌에는 염전이 발달한다. 또한 하나의 갯벌에도 경제 활동의 분화가 이루어져 조상대에는 염전과 양어장으로, 조간대에는 패류 양식장으로, 조하대는 굴, 김 양식징으로 이용된다.

• 농촌계획
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• 제25권4호
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• pp.47-56
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• 2019

More than half of the nonpoint sources of polluting water occur in cultivating farmlands in rural areas. Agricultural nonpoint sources are discharged from large areas of farmlands, making it difficult to collect or treat pollutants. Farmland source management is known to be the most effective, and preventive management by improving farming methods is the key to reduce nonpoint pollution. At present, more than 30% of the pollutants flowing into the rivers and lakes are nonpoint pollutants caused by agricultural activities. As a countermeasure, it is more preferable to develop and apply optimal farming management techniques for agricultural nonpoint pollution management basically than to apply existing water quality management techniques. Because of the characteristics of nonpoint source pollution, it is necessary to manage farmlands in rural areas, so the willingness and competence of the residents is most important. The purpose of this study is to analyze and understand the process of changing the cognition of residents through capacity education and survey for nonpoint pollution management in rural areas. This study conducted intensive resident competency education and examined the process of changing resident awareness through three surveys. As a result of this study, it was found that continuous education and activities for rural non-point pollution management are necessary for raising awareness of residents and managing non-point pollution effectively, showing possibility of change residents' perception.

• Safety and Health at Work
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• 제12권4호
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• pp.432-438
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• 2021

Background: We aimed to investigate the prevalence of low back pain (LBP) and its associated agricultural work-related, biomechanical factors among this population. Methods: We analyzed initial survey data from the Safety for Agricultural Injury of Farmers cohort study involving adult farmers in Jeju Island. The prevalence of LBP was calculated with associated factors. Results: In total, 1,209 participants were included in the analysis. The overall prevalence of LBP was 23.7%. Significant associations for LBP were the type of farming activity, length of farming career, prior agricultural injury within 1 year, and stress levels. Multivariate logistic regression analysis revealed three biomechanical factors significantly related to LBP: repetitive use of particular body parts; the inappropriate posture of the lower back and neck. Conclusions: Some occupational, and biomechanical risk factors contribute to LBP. Therefore, postural education, injury prevention education, and psychological support will be needed to prevent LBP.

• 한국환경과학회지
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• 제32권12호
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• pp.955-964
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• 2023

This study was conducted to obtain basic data on efficient and eco-friendly crop cultivation for urban residents who enjoy urban agriculture as a hobby or leisure activity. We planted strawberry(Fragaria x ananassa)(S) and kidney bean (Phaseolus vulgaris var. humilis)(K) in different ratios to analyze the differences in soil environment, growth, physiology, and productivity, and to investigate the effects. Strawberry growth was optimal with S1K2 ratio, whereas the S1K1 ratio treatment showed the highest levels of physiology and productivity. In terms of growth and physiology, kidney beans tended to perform best in S1K2 treatment. The average number of productive pods was two, with a highest average value of 2.3 being recorded in S2K1 treatment planted with a high percentage of strawberries. In terms of growth, physiology, and prodctivity our findings indicate that it would be desirable to plant starwberries and kidney bean in a 1:1 ratio. However, considering the environmental characteristics of walls and rooftops, it is necessary to effectively manage crops suitable for these conditions. Furthermore, additional studies should be conducted to analyze the quality of fruits and seeds producted, both qualitatively and quantitatively in the future.