Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
권호 표지
DOI QR코드

DOI QR Code

Antioxidant, Antimicrobial Acitivities and Cytotoxicity of Hot Water Extracts of Major Herbs in Korea

국내산 주요허브 열수 추출물의 항산화, 항균활성 및 세포독성

  • 강다래 (전북대학교 농업생명과학대학 동물생명공학과) ;
  • 심관섭 (전북대학교 농업생명과학대학 동물생명공학과) ;
  • 최호성 (전북대학교 농업생명과학대학 동물생명공학과) ;
  • 나종삼 (전북대학교 농업생명과학대학 동물생명공학과)
  • Received : 2017.08.29
  • Accepted : 2017.11.16
  • Published : 2017.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, the DPPH free radical scavenging activity, antimicrobial effects, growth inhibition and cytotoxicity of herb extracts were determined to screen alternative antibiotics. Hot water extracts of 10 species herbs (Origanum vulgare, Monarda didyma, Echinacea purpurea, Ocimum basilicum, Mentha piperita, Melissa officinalis, Thymus vulgaris, Stevia rebaudiana, Rosmarinus officinalis, Matricaria recutica) were used. DPPH free radical scavenging activity of all herb extracts was ranged from 31.4-49.9%, and significantly great activties were found at Rosmarinus officinalis, Origanum vulgare and Matricaria recutica (P<0.05). Hot water extracts of monarda didyma, origanum vulgare, thymus vulgaris and rosmarinus officinalis showed greater antimicrobial activities compared to others. Additionally, those four extracts represented relatively low cytotoxicity compared to others. As a result, it was found that Origanum vulgare and Rosmarinus officinalis which possessed great antioxidant and antimicrobial activity with less cytotoxicity. So these two herb extracts can be used as an alternative of antibiotics for organic farming.

본 연구는 10종 허브 추출물에 대한 DPPH 자유라디칼 소거능, 항균효과 및 세포독성에 미치는 영향을 조사함으로써 항생제 대체제로서 활용 가능성을 위한 기초 자료로 제시하고자 수행하였다. 10종의 허브 추출물의 DPPH 자유라디칼 소거능은 로즈마리, 오레가노 및 캐모마일이 각 49.9, 48.9, 47.7%으로 유의적으로 가장 높았다. 허브 추출물의 E. coli K99와 S. typhimurium에 대한 항균활성은 베르가못, 오레가노, 타임과 로즈마리가 두 균주에 공통적으로 활성이 높게 측정되었으며, 활성이 나타나는 농도에서의 세포독성이 비교적 낮아 안전한 것으로 관찰되었다. 본 연구 결과 유기 축산을 위한 가축 내 천연 항생제 대체제로 항균 및 항산화 활성이 높고, 세포독성이 낮게 나타난 오레가노와 로즈마리가 천연물질로 활용 가능할 것으로 판단되며, 다양한 추출 조건으로 최적 추출 효율을 검토하여 추가적인 생리활성물질 검정과 해당 물질에 대한 연구가 필요하다.

Keywords

References

  1. Berrington, D. and N. Lall. 2012. Anticancer activity of certain herbs and spices on the cervical epithelial carcinoma (HeLa) cell line. Evid. Base. Compl. Alternat. Med. 564927: 1-11.
  2. Chang, W. K., S. B. Cho, D. W. Kim, S. S. Lee, and S. K. Kim. 2010. Cell growth and antioxidant activity on onion juice fermentation by using Lactobacillus plantarum as animal probiotics. J. Life Sci. 20(11): 1729-1737. https://doi.org/10.5352/JLS.2010.20.11.1729
  3. Chea, I. G., H. J. Kim, M. H. Yu, H. I. Kim, and I. S. Lee. 2010. Antioxidant and antibacterial activity of commercially available herbs in Korean markets. Kor. J. Soc. Food Sci. 39(10): 1411-1417. https://doi.org/10.3746/jkfn.2010.39.10.1411
  4. Choi, I. Y., Y. J. Song, and W. H. Lee. 2010. DPPH radical scavenging effect and antimicrobial activities of some herbal extracts. Kor. J. Hort. Sci. Technol. 28(5): 870-876.
  5. Chung, D. O., I. D. Park, and H. O. Jung. 2001. Evaluation of functional properties of onion, rosemary, and thyme extracts in onion kimchi. Korean J. Food Cook. Sci. 17(3): 218-223.
  6. Duncan, D. B. 1955. Multiple range and multiple F tests. Biometrics. 11(1): 1-42. https://doi.org/10.2307/3001478
  7. Hammer, K. A., C. F. Carson, and T. V. Riley. 1999. Antimicrobial activity of essential oils and other plant extracts. J. Appl. Microbiol. 86(6): 985-990. https://doi.org/10.1046/j.1365-2672.1999.00780.x
  8. Ji, J. R., Y. M. Choi, D. Y. Song, H. S. Choe, C. S. Na, and K. S. Shim. 2011. Effect of feeding peppermint (Mentha piperita L.) powder on meat quality and fatty acid composition in finishing Korean native black pigs. Korean J. Food Sci. Ani. Resour. 31(2): 224-231. https://doi.org/10.5851/kosfa.2011.31.2.224
  9. Kang, B. K., K. B. W. R. Kim, N. K. Ahn, Y. U. Choi, M. J. Kim, S. W. Bark, W. M. Pak, B. R. Kim, J. H. Park, N. Y. Bae, and D. H. Ahn. 2015. Anti-inflammatory effect of onion (Allium cepa) peel hot water extract in vitro and in vivo. Korean. Soc. Biotechnol. Bioeng. 30(4): 148-154.
  10. Kang, S. N., J. D. Kim, I. S. Kim, S. K. Jin, and M. H. Lee. 2007. Effect of replacing antibiotics by herb extracts and digestive enzymes containing vitamin E and oriental medicinal plants byproduct on blood serum cholesterol and meat qualities in the hog loin meat. Korean J. Food. Sci. Ani. Resour. 27(1): 87-94. https://doi.org/10.5851/kosfa.2007.27.1.87
  11. Kim, D. H., K. H. Kim, I. S. Nam, S. S. Lee, C. W. Choi, W. Y. Kim, E. G. Kwon, K. Y. Lee, M. J. Lee, and Y. K. Oh. 2013. Effect of indigenous herbs on growth, blood metabolites and carcass characteristics in the late fattening period of hanwoo steers. Asian-Australas. J. Anim. Sci. 26(11): 1562. https://doi.org/10.5713/ajas.2013.13132
  12. Kim, I. S., M. R. Yang, O. H. Lee, and S. N. Kang. 2011. Antioxidant activities of hot water extract from various spices. Int. J. Mol. Sci. 12(6): 4120-4131. https://doi.org/10.3390/ijms12064120
  13. Lee, A. L., H. R. Park, M. S. Kim, S. B. Cho, and N. J. Choi. 2014. A comparative study between microbial fermentation and non-fermentation on biological activities of medicinal plants, with emphasis on enteric methane reduction. Korean J. Organic Agri. 22(4): 801-813. https://doi.org/10.11625/KJOA.2014.22.4.801
  14. Omar, J. A., A. Hejazi, and R. Badran. 2016. Performance of broilers supplemented with natural herb extract. Open J. Anim. Sci. 6(1): 68-74. https://doi.org/10.4236/ojas.2016.61009
  15. Park, H. W., K. H. Jang, M. Hussain, and D. J. Lee. 2012. Evaluation of 1,1-diphenyl-2-picrylhydrazyl radical scavenging effect, cytotoxicity and tyrosinase inhibition activities in 4 species of herb plants. J. Appl. Biol. Chem. 55(4): 201-205. https://doi.org/10.3839/jabc.2012.032
  16. Petersen, M. B., K. Soegaard, and S. K. Jensen. 2011. Herb feeding increases n-3 and n-6 fatty acids in cow milk. Livest. Sci. 141(1): 90-94. https://doi.org/10.1016/j.livsci.2011.05.004
  17. Shim, K. S., C. S. Na, S. J. Oh, and N. J. Choi. 2012. Optimization of aqueous methanol extraction condition of total polyphenol from spent Lycium chinense miller to develop feed additives for pig. Korean J. Organic agri. 20(1): 91-99.
  18. Wanapat, M., A. Cherdthong, P. Pakdee, and S. Wanapat. 2008. Manipulation of rumen ecology by dietary lemongrass (Cymbopogon citratus Stapf.) powder supplementation. J. Anim. Sci. 86(12): 3497-3503. https://doi.org/10.2527/jas.2008-0885
  19. Yesil-Celiktas, O., C. Sevimli, E. Bedir, and F. Vardar-Sukan. 2010. Inhibitory effects of rosemary extract, carnosic acid and rosmarinic acid on the growth of various human cancer cell lines. Plant Food. Hum. Nutr. 65(2): 158-163. https://doi.org/10.1007/s11130-010-0166-4
  20. Yu, M. H., I. G. Chae, Y. T. Jung, Y. S. Jeong, H. I. Kim, and I. S. Lee. 2011. Antioxidative and antimicrobial activities of methanol extract from Rosmarinus officinalis L. and their fractions. J. Life Sci. 21(3): 375-384. https://doi.org/10.5352/JLS.2011.21.3.375