• 한국동물위생학회지
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• 제26권2호
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• pp.163-184
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• 2003

This study was conducted to survey the farm which suffered from disease similar to classical swine fever(CSF) in Gyeongbuk province. Clinical signs appeared first in a few number of growing pigs which showed specific signs of diarrhea, depression, sleepiness, and reluctance to get up or to eat. Younger piglets may have appeared chilled, shiver and huddle together. As the disease progresses the affected pig's skin went red and purple. In histopathological signs, there were many haemorrhages throughout the body and larger haemorrhages in some organs such as lymph nodes. And there is a precipitous fall in the number of circulating leukocytes in the blood. In spite of insisting of farmer which did not vaccinate to classical swine fever, significant antibody production was detected in these affected pigs at enzyme-linked immuonsorbent assay. According to the above results at first glance, these affected pig suspected with CSF in clinical signs and histopathological lesions only. Because the symptoms and post-mortem picture were very similar to CSF, these false positive results would have been dangerous to diagnostician. But by reverse transcriptase polymerase chain reaction(RT-PCR) and comparative nucleotide sequence analysis, the disease was correctly diagnosed with post-weaning multisystemic wasting syndrome(PMWS) and porcine reproductive and respiratory syndrome(PRRS) compoundly. And the antigen which were detected the lesion similar to CSF virus, was confirmed with LOM vaccine strain of CSF. In most national CSF eradication program and in countries which are free of the CSF virus, vaccination against CSF is not practiced and generally is not allowed. But now in Korea, routine vaccination is practiced because of outbreaking the CSF repeatedly. When CSF is diagnosed the whole herd and other in contact animal are slaughtered continuously.

• 한국동물위생학회지
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• 제31권4호
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• pp.493-496
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• 2008

Brucella spp. are small, non-motile Gram-negative coccobacilli known to cause disease in a number of vertebrate species including humans and brucellosis is one of the world's major zoonoses, alongside bovine tuberculosis and rabies. There are about 33.55 million goats and 1.16 million sheep in Bangladesh. The sheep and goats can significantly play an important role in the economic well being of the resource-poor farmer in Bangladesh. Sexually matured 362 female small ruminants(300 goats and 62 sheep) were examined. Approximately 3-5 ml of blood was collected from the jugular vein of each animal and sera samples were prepared. Samples were then tested for brucellosis by using Rose Bengal test(RBT), plate agglutination test(PAT) and tube agglutination test(TAT). Among 362 small ruminants, irrespective of species(sheep or goat), diagnosed highest in TAT, 2.21%(n=8) and lowest both by RBT & PAT, 1.93%(n=7) and it is concluded that TAT is superior than RBT and PAT.

• Journal of Animal Science and Technology
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• 제57권3호
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• pp.9.1-9.5
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• 2015

Background: A simple binary scoring system (SBSS) was developed and used to assess the welfare measures of commercial broiler chickens in South Korea. We also correlated welfare measures with environmental parameters of broiler house. Our measures of welfare included lameness, hock burn (HB) and foot pad dermatitis (FPD), whilst environmental parameters included air temperature, relative humidity, air speed, light intensity, air quality (in particular carbon dioxide ($CO_2$) and ammonia ($NH_3$) concentrations) and airborne microbes. Results: The effect of environmental parameters on welfare measures was apparent even on 10-day-old broilers. A non-parametric correlation analysis revealed significant correlations between environmental parameters and welfare measures. The key environmental parameters were relative humidity and light intensity. The results indicate that there is a need for proper control of environmental conditions on poultry farms, which could reduce health problems and subsequently reduce disease and mortality. Conclusions: In conclusion, the simplicity of SBSS makes it preferable over more complex scoring systems and allows a farmer to more easily assess the welfare measures on their own farm.

• 농업과학연구
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• 제42권4호
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• pp.389-396
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• 2015

This study was performed to investigate the opinions of various related-industries on ban of antibiotics growth promoters (AGPs) in commercial mixed feed. The answers on a total of 21 questions were summarized by response number and percentage. 93% of those surveyed were in agreement of a ban of dietary AGPs. The agreement reasons were the livestock safety (61.5%), the reduction of antibiotic use (23.1%), and decrease of antibiotic-resistant bacteria (11.5%). The negative effects expected by the ban of AGPs were poor growth performance (44.2%), elevated disease emergence (31.4%), increasing the feed cost (18.6%), and quality degradation of livestock (5.8%). As the efficient plans for decline of AGPs use, the feeding environment improvement was the highest with 43%, and farmer training and the consolidation inspection of residual substance on antibiotics in livestock product was 27.9% and 22.1%, respectively. 46.5% of respondent are considering the modification of feed spec and 39.5% of those surveyed have staged a modified feed spec. In conclusion, livestock related-industries approve a ban of AGPs, and they assert that the policy support, improvement of management and environment in the farm, providing technology from related-industries are multiply essential for a stable settlement of a ban policy of AGPs.

• 식물병연구
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• 제8권4호
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• pp.215-219
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• 2002

2002년 7월 경남 통영시 광도면 석류재배 농가포장에서 열매가 부패되는 이상증상이 발생하였다. 분리된 병원균은 감자한천배지에서 균총의 색깔은 흰색이며 물결모양을 이루면서 왕성하게 자랐고, 배지상에서 분생포자각을 잘 형성하였다. 분생포자는 단세포이며 모양은 방추형이고, 색깔은 갈색 또는 올리브색이다. 크기는 10.3~17.4$\times$2.8~4.0 $\mu\textrm{m}$ 였다. 분생포자 형성 세포는 짧은 원통형이며 무색으로 크기는 12.4~1.4$\times$2.8~3.6 $\mu\textrm{m}$ 였다. 불생포자각의 모양은 구형이며 갈색으로, 각벽은 2~3세포이고, 크기는 124.6~228.4 $\mu\textrm{m}$ 였다. 균사생육 적온은 $25^{\circ}C$였다. 분리된 병원균을 건전한 석류에 유상, 무상접종한 결과 접종 3일 후 감염을 일으켰으며, 병원성이 강하였다. 이상과 같이 이 병원균을 Coniella granati에 의한 석류열매 썩음병으로 명명할 것을 제안한다.

• 식물병연구
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• 제12권3호
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• pp.284-286
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• 2006

경남 진주시 정촌면 강주연못, 금산면 가정집 정원수로 심은 영산홍 잎에 떡병이 심하게 발생하였다. 병징은 잎에 발생하여 흰색의 가루같은 자실층이 생기고 이상비대를 한다. 병반부에서 자실체가 잎 앞, 뒷면으로 노출되고 흰색의 전형적인 떡모양으로 된다. 담자기는 무색, 곤봉 모양의 원주형이며 $1{\sim}5$개 정도의 작은 자루모양을 하고 있으며 크기는 $8{\sim}35{\times}3{\sim}6{\mu}m$였다. 담자포자는 무색, 단포이며 장타원형 모양의 원주형으로 아랫부분은 약간 굽었으며 크기는 $10{\sim}22{\times}3{\sim}5{\mu}m$였다. 영산홍에서 병원성을 검정하였다. 잎에 발생한 병징과 병원균의 균학적 특징을 조사한 결과, 이 병을 Exobasidium japonicum Shirai에 의한 영산홍 떡병으로 명명하여 국내에 처음 보고한다.

• 한국균학회지
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• 제28권2호
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• pp.121-122
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• 2000

경상남도농업기술원 함양약초시험장 포장에 발생한 병든 둥굴레로부터 병원균을 분리하여 병원균의 특성과 병징을 토대로 Sclerotium rolfsii에 의한 둥굴레 흰비단병을 동정하였다. 이 병의 병징은 지제부가 암갈색으로 변하고 그 위에 흰색의 곰팡이가 솜털처럼 밀생하고, 병반부위에 갈색의 둥근 균핵을 많이 형성하며 지제부 부근의 토양 표면에도 형성되었다. 병원균은 PDA 배지 상에서 왕성하게 생장하였으며 균사생장 중에 특유의 Clamp connection이 관찰되었고 균사가 가장 잘 자랄수 있는 온도는 $30^{\circ}C$이였다. PDA 배지에서 시간이 경과되면 갈색을 띤 구형 또는 부정형의 많은 균핵을 형성하였는데 균핵의 크기는 $0.9{\sim}6.8{\times}0.6{\sim}5.2\;mm$ (평균 $2.7{\sim}2.3\;mm$) 이었다.

• 대한인간공학회지
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• 제35권2호
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• pp.67-74
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• 2016

Objective: The aim of this study is to develop a farming shovel to reduce workload, which helps farmers lower the risk of musculoskeletal disease. Background: Most of work using farming tools including shovels requires repetitive works and awkward postures on body parts, and it could possibly cause work-related musculoskeletal disorders. It is necessary to develop and distribute farm equipment and tools in order to reduce physical workload. Method: To improve the most uncomfortable task perceived by ten farmers during the work with a shovel, the improved shovel was designed and made as a prototype for experiment for the comparison of the existing and improved shovels. Twenty males were recruited for this experiment, and muscle activity (%MVC) of six body parts and subjective discomfort ratings by body parts while working with a shovel were measured. A paired t-test was performed to compare physical workload between the existing shovel and the developed one. Results: A shovel applying foothold tied between shaft and blade was designed, which can help workers reduce repetitive bending of back and pressures for upper limbs while digging soil. According to compared evaluation of the developed shovel and the existing shovel, the developed shovel's %MVCs in all experimental muscles were significantly lower than those of the existing shovel. The developed shovel showed the biggest drop in perceived subjective discomfort rating of back, followed by arm and neck, compared to the existing shovel. Conclusion: It was confirmed that attaching a foothold to a shovel was an effective way of reducing workload in back and upper limbs during digging. Application: In the near future, if we put the prototype of developed shovel to practical use after making up for defects, it will help farm work environment be healthier and safer.

• 한국유기농업학회지
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• 제27권1호
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• pp.1-16
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• 2019

The questionnaire survey was conducted on 225 farmers in Gyeonggi-do, Jeollanam-do and Jeollabuk-do. A total of 189 (84%) farmers responded. 72% of the respondents were males, 50.3% were aged 60 or older, and 51.3% had less than 5 years of farming experience. 78.8% of the respondents are pesticide-free, and 44.4% of respondents have less than 0.5 ha of farming scale. 61.4% of the cultivated crops were vegetable crops. The order of seeds and seedlings to buy was tomato (23.3%), cucumber (12.2%) and pepper (10.6%). The cost of purchasing seeds ranged from a minimum of 100,000 won to a maximum of 5 million won. 78.3% of respondents answered that they well-knew or knew about organic seeds. 78.3% of respondents answered that they knew or knew about organic seeds. Of the positive effects of mandatory use of organic seeds, 41.3% of respondents said they would increase confidence in organic certification. However, 41% of respondents who opposed the mandatory use of organic seeds said that "The strengthening of regulations will make organic agriculture more difficult." When the use of organic seeds is mandatory, 43.4% of the respondents favor direct support for the purchase of organic seeds, which should be supported politically by the state. When organic seeds were supplied, the disease resistant seeds (53.4%) was the preferred characteristic of organic seeds. For the optimal price of organic seeds, 38.6% of respondents wanted the same price as the commercialized conventional seed. In this study, the questionnaire was conducted for three major organic farming regions, but many of the respondents were judged to have a legal position on the mandatory use of organic seeds. Therefore, the results of this study can be used as a basic data for reviewing the legislation on the organic seed production and distribution suitable for the situation of Korean organic farming.

• 한국동물생명공학회지
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• 제36권1호
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• pp.35-44
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• 2021

A pregnancy diagnosis is an important standard for control of livestock's reproduction in paricular dairy cattle. High reproductive performance in dairy animals is a essential condition to realize of high life-time production. Pregnancy diagnosis is crucial to shortening the calving interval by enabling the farmer to identify open animals so as to treat or re-breed them at the earliest opportunity. MicroRNAs are short RNA molecules which are critically involved in regulating gene expression during both health and disease. This study is sought to establish the feasible of circulating miRNAs as biomarkers of early pregnancy in cattle. We applied Illumina small-RNA sequencing to profile miRNAs in plasma samples collected from 12 non-pregnant cows ("open" cows: samples were collected before insemination (non-pregnant state) and after pregnancy check at the indicated time points) on weeks 0, 4, 8, 12 and 16. Using small RNA sequencing we identified a total of 115 miRNAs that were differentially expressed weeks 16 relative to non-pregnancy ("open" cows). Weeks 8, 12 and 16 of pregnancy commonly showed a distinct increase in circulating levels of miR-221 and miR-320a. Through genome-wide analyses we have successfully profiled plasma miRNA populations associated with pregnancy in cattle. Their application in the field of reproductive biology has opened up opportunities for research communities to look for pregnancy biomarker molecules in dairy cattle.