• KIEAE Journal
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• 제12권2호
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• pp.93-101
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• 2012

In this study, we measured the Index of Greeness(I.O.G) and analyzed factors that affect in downtown street of Seoul. The aim of this article is to provide an ways of improving I.O.G, with an emphasis on accuracy of measurement. We utilized the autimatic measurment techniques proposed by Tonosaki to solve the problem of accuracy in measurement. The result is as follows : Firstly, the average I.O.G of Gwanghwamun Plaza is 15.76%, Jongno is 11.48% and Dasanro(from Yaksu station to Beotigogae station) is 6.36%. According to the comprehensive analysis in three reseach areas, it shows that I.O.G is intimately linked with planting method, species of trees and the presence of wall planting. Secondly, it was analyzed that grass and ground-cover planting promote I.O.G better than other method. The I.O.G of photo which contains grass is 45.47%. According to the comparative analysis between tree planting and multi-planting method, the presence of lower planting showed a difference about 8.77% of I.O.G.. From the persipective of I.O.G, tree planting with two lines is more effective than one. The difference of two ways is about 3.24%. Thirdly, it is an efficint way to use the wall planting or vertical planting in order to promote I.O.G.. In Dasanro, The I.O.G of photo which contains wall planting or vertical planting is 45.47% in contrast to the average of I.O.G. is 6.36%. Fourthly, the difference of I.O.G between broadleaf tree and needleleaf tree was larger than we thought. If look closely confined to this study, the I.O.G of street planted by Pine is 3.61% and Eastern Sycamore Family Bottonwood is 12.55%.

• Asian-Australasian Journal of Animal Sciences
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• 제21권5호
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• pp.663-670
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• 2008

The effect of supplementing the diet of grazing dairy cows with fish oil (FO) and linseed oil (LSO) on milk conjugated linoleic acid (CLA) was investigated. Sixteen Holstein cows (17019 DIM) were assigned into two groups and fed a grain supplement (8.0 kg/d; DM basis) containing 800 g of saturated animal fat (CONT) or 200 g FO and 600 g LSO (FOLSO). All cows grazed together on Sudan grass pasture ad libitum and were fed the treatment diets for 3 wks. Cows were milked twice a day and milk samples were collected during the last three days of the trial. Milk production (24.89 and 22.45 kg/d), milk protein percentage (2.76 and 2.82) and milk protein yield (0.68 and 0.64 kg/d) for the CONT and FOLSO diets, respectively, were not affected (p>0.05) by treatment diets. Milk fat percentage (3.90 and 2.86) and milk fat yield (0.97 and 0.64 kg/d) were lower (p<0.05) with the FOLSO diet compared with the CONT diet. The concentration and yield of milk cis-9 trans-11 CLA were higher (p<0.05) with the FOLSO diet (2.56% of total FA and 16.44 g/d, respectively) than the CONT diet (0.66% of total FA and 6.44 g/d, respectively). The concentrations of milk trans C18:1 and vaccenic acid (VA) were higher (p<0.05) with the FOLSO diet (13.53 and 7.48% of total FA, respectively) than the CONT diet (3.69 and 2.27% of total FA, respectively). In conclusion, supplementing the diet of grazing cows with FO and LSO increased milk cis-9 trans-11 CLA content but reduced milk fat content and yield.

• BMB Reports
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• 제40권3호
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• pp.358-367
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• 2007

A novel mannose-binding tuber lectin with in vitro antiproliferative activity towards human cancer cell lines and antiviral activity against HSV-II was isolated from fresh tubers of a traditional Chinese medicinal herb, Typhonium divaricatum (L.) Decne by a combined procedure involving extraction, ammonium sulfate precipitation, ion exchange chromatography on DEAE-SEPHAROSE, CM-SEPHAROSE and gel-filtration on sephacryl S-200. The apparent molecular mass of the purified Typhonium divaricatum lectin (TDL) was 48 kDa. TDL exhibits hemagglutinating activity toward rabbit erythrocytes at 0.95 $\mu$g/ml, and its activity could be strongly inhibited by mannan, ovomucoid, asialofetuin and thyroglobulin. TDL showed antiproliferative activity towards some well established human cancer cell lines, e.g. Pro-01 (56.7 $\pm$ 6.8), Bre-04 (41.5 $\pm$ 4.8), and Lu-04 (11.4 $\pm$ 0.3). The anti-HSV-II activity of TDL was elucidated by testing its HSV-II infection inhibitory activity in Vero cells with $TC_50$ and $EC_50$ of 5.176 mg/ml and 3.054 $\mu$g/ml respectively. The full-length cDNA sequence of TDL was 1145 bp and contained an 813-bp open reading frame (ORF) encoding a 271 amino acid precursor of 29-kDa. Homology analysis showed that TDL had high homology with many other mannose-binding lectins. Secondary and three-dimensional structures analyses showed that TDL is heterotetramer and similar with lectins from mannose-binding lectin superfamily, especially those from family Araceae.

• Preventive Nutrition and Food Science
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• 제11권2호
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• pp.105-109
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• 2006

Pinus densiflora belongs to the Pinaceae family which has been widely used for health promoting purposes as folk medicine or as a food. Various curative effects of different parts of the pine have been reported including as a remedy for carcinoma. We examined the effects of pine needle water extracts (PNE) on macrophage function using peritoneal macrophage, pre-osteoclast bone macrophage (Raw 264.7 cell) and brain macrophage (C6 microglia). When peritoneal macrophages were treated with various concentrations of PNE ($1{\sim}100{\mu}g/mL$) for 24 hours, phagocytic activity was significantly increased, whereas it had no effect on tumoricidal activity and NO production. However, the treatment of Raw 264.7 with PNE resulted in the enhancement of NO production at high concentration ($100{\mu}g/mL$). Furthermore, the treatment of C6 with PNE increased the production of NO in a concentration-dependent manner, whereas PNE suppressed NO production in $LPS/IFN-{\gamma}-stimulated$ microglia. These results suggest that PNE has differential immunomodulatory effects on macrophages.

• The Journal of Asian Finance, Economics and Business
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• 제7권11호
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• pp.251-257
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• 2020

The study investigates the role of commodity prices and tax purpose recognition on bitcoin prices. Since the introduction of bitcoin in 2008, emphasis has focused on economists, policy-makers and analysts drastically increasing bitcoin's accessibility and commodity values (Dumitrescu & Firică, 2014). This study employs GARCH and EGARCH from ARCH/GARCH family on daily nature data. We measure the volatile behavior of bitcoin by employing auto-regressive conditional heteroscedasticity model with the aim to explore the relationship between major commodities and bitcoin volatility. We focus on major commodities like gold, silver, platinum, and crude oil to be regressed with bitcoin. The daily prices of commodities were retrieved from www.investing.com and bitcoin prices from www.coindesk.com for the period from 29April 2013 to 16 October 2018. Results confirmed the currency's long-term volatile behavior, which is due to its composition and market dynamics, whereas the existence of asymmetric information effect is not confirmed. Tax recognition by other countries may in future help in controlling the volatility as bitcoin is not a country-specific security. But, only silver impacts on volatility in comparison to oil prices and platinum, which is due to its similar features with gold. Eventually, bitcoin can be used for risk diversification and money making.

• 생명과학회지
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• 제22권7호
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• pp.863-870
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• 2012

세포막 단백질 중 가장 큰 family를 형성하는 G protein-coupled receptor (GPCR)는 세포 외부의 다양한 신호를 세포 내 G 단백질의 활성화를 통하여 전달한다. 외부 신호자극이 없는 조건에서도 활성을 나타내는 항활성 돌연변이(constitutively active mutants, CAM)는 GPCR 신호전달 이상으로 인한 질병 치료나 GPCR의 활성화 구조연구에 좋은 대상이다. 본 연구는 시각수용체 로돕신에서 약한 항활성을 보이는 CAM의 하나인 E134Q/M257Y를 대상으로, inverse agonist와 agonist 존재 하에서 형성하는 두 가지 chromophore의 특성을 연구하였다. 이 CAM은 11-cis-retinal과 all-trans-retinal 존재 하에서 각기 최대흡광도가 500 nm와 380 nm인 로돕신을 형성한다. 두 가지 retinal을 다양한 비로 혼합한 조건과 연속적으로 결합하는 조건 하에서 각 형태의 로돕신 형성을 조사한 결과 E134Q/M257Y mutant는 11-cis-retinal과 우선적으로 결합함을 보여준다. E134Q/M257Y mutant는 wild type 옵신에 비해 11-cis-retinal에 대한 친화도는 별다른 차이가 없으나 옵신과 로돕신 상태의 안정성이 낮음이 확인되었다. 본 연구 결과는 GPCR의 활성화 시 일어나는 부분적 구조변화에 대한 정보를 제공하고, 구조정보에 기반한 GPCR신호를 미세하게 조절하는 물질의 발굴이나 개발에 유용하게 이용될 것이다.

• 한국식생활문화학회지
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• 제12권4호
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• pp.419-421
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• 1997

Washing water volume of rice for cooking and rice weight for one person per meal in one household were surveyed for confirming pollution by the washing water and total losses by rice washing procedures. The mean size of a family was 4.64 persons and the mean consumption weight of rice per capital per meal was 138.43g. It is used 0.782 L of water for washing the rice per capital share. The washing water of rice was composed of 0.32% of total solid, 0.11% of soluble solid and 1.65% of solid loss to rice, and COD and BOD of it were 2.400 and 3.564 ppm respectively. Based on total population in Korea, 41 miliion, excepting age below 4, total cost for washing water of rice summed up about 1,495 billion won including 8.8 billion won for tap water cost, 11.7 billion won for waste water treatment, and 129 billion for solid loss of rice.

• 생명과학회지
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• 제17권1호
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• pp.6-11
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• 2007

당 분해효소의 family 32 (GH32)에 속하는 $\beta-fructofuranosidase$는 3차구조를 근거로 볼 때 W(L/M)(C/N)DP(Q/N), FRDPK, 그리고 ECP(D/G) 부위를 포함하는 세 군데의 보전적인 영역을 가지고 있다. 이러한 $\beta-fructofuranosidase$ family에 속하는 Microbacterium sp. AL-210 유래 levan fructotransferase (LFTase)의 보전적인 산성 아미노산들의 역할이 특정위치 돌연변이법으로 검사되었다. 각각의 돌연변이체는 대장균인 E. coli BL21 (DE3)균주에서 발현되어 대량 생산되었고, 금속 친화 크로마토그래피법과 FPLC법으로 순수 정제되었다. wild-type LFTase의 효소의 활성은 0.74 unit 인 반면 네 개의 돌연변이체인 D63A, D195N, E245A, E245D 각각은 specific activity를 측정해 본 결과 원 균주와 비교해서 약 100배 정도 감소한 효소활성을 보여 주었다. 이로써 아미노산 변형의 target이 되었던 Asp-63, Aps-195, 그리고 Glu-245가 모두 효소 활성 및 기질과의 결합에 상당히 중요한 역할을 하고 있음이 판명되었다. 이러한 세 부위의 산성 아미노산들은 inulinase, levan fructotransferase와 invertase에 모두 보전적으로 위치 하므로 이들은 $\beta-fructofuranosidase$ family내 에서 공통된 역할을 할 것으로 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6601-6604
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• 2013

Aim: Associations between polymorphisms in miR-146aG>C, miR-196a2C>T and miR-499A>G and risk of HCC, and interaction with HBV infection in a Chinese population, were the target of the present research. Methods: The duplex polymerase-chain-reaction with confronting-two-pair primers (PCR-RFLP) was performed to determine the genotypes of the miR-146aG>C, miR-196a2C>T and miR-499A>G genotypes. Associations of polymorphisms with the risk of HCC were estimated by conditional logistic regression analysis. Results: Drinking, family history of cancer, HBsAg and HCV were risk factors for HCC. Multivariate regression analyses showed that subjects carrying the miR-196a2 CC genotype had significantly increased risk of HCC, with an adjusted OR (95% CI) of 2.18 (1.23-3.80). In addition, cases carrying the miR-196a2 C allele had a 1.64-fold increase in the risk for HCC (95%CI=1.03-2.49). The miR-196a2 CT and TT genotypes greatly significantly increased the risk of HCC in subjects with HBV infection, with adjusted ORs (95% CI) of 2.02 (1.12-3.68) and 2.69 (1.28-5.71), respectively. Conclusion: Our results demonstrate that miR-196a2 CC genotype and C allele have an important role in HCC risk in Chinese, especially in patients with HBV infection.

• Membrane and Water Treatment
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• 제11권1호
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• pp.41-48
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• 2020

Lead is a highly toxic heavy metal that causes serious health problems. Nonetheless, it is increasingly being used for industrial applications and is often discharged into the environment without adequate purification. In this study, Pb(II) was removed by powdered waste sludge (PWS) based on the biosorption mechanism. Different PWSs were collected from a submerged moving media intermittent aeration reactor (SMMIAR) and modified Ludzack-Ettinger (MLE) processes. The contents of extracellular polymeric substances were similar, but the surface area of MLE-PWS (2.07 ㎡/g) was higher than that of SMMIAR-PWS (0.82 ㎡/g); this is expected to be the main parameter determining Pb(II) biosorption capacity. The Bacillaceae family was dominant in both PWSs and may serve as the major responsible bacterial group for Pb(II) biosorption. Pb(II) biosorption using PWS was evaluated for reaction time, salinity effect, and isotherm equilibrium. For all experiments, MLE-PWS showed higher removal efficiency. At a fixed initial Pb(II) concentration of 20 mg/L and a reaction time of 180 minutes, the biosorption capacities (q_e) for SMMIAR- and MLE-PWSs were 2.86 and 3.07 mg/g, respectively. Pb(II) biosorption using PWS was rapid; over 80% of the maximum biosorption capacity was achieved within 10 minutes. Interestingly, MLE-PWS showed enhanced Pb(II) biosorption with salinity values of up to 30 g NaCl/L. Linear regression of the Freundlich isotherm revealed high regression coefficients (R² > 0.968). The fundamental Pb(II) biosorption capacity, represented by the K_F value, was consistently higher for MLE-PWS than SMMIAR-PWS.