• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.3
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• pp.155-162
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• 2002

The cyclic amidohydrolase family enzymes, which include allantoinase, dihydroorotase, dihydropyrimidinase and (phenyl)hydantoinase, are metal-dependent hydrolases and play a crucial role in the metabolism of purine and pyrimidine in vivo. Each enzyme has been independently characterized, and thus well documented, but studies on the higher structural traits shared by members of this enzyme family are rare due to the lack of comparative study. Here, we report upon the expression in E. coli cells of maltose-binding protein (MBP)- and glutathione S-transferase (GST)-fused cyclic amidohydrolase family enzymes, facilitating also for both simple purification and high-level expression. Interestingly, the native quaternary structure of each enzyme was maintained even when fused with MBP and GST. We also found that in fusion proteins the favorable biochemical properties of family enzymes such as, their optimal pHs, specific activities and kinetic properties were conserved compared to the native enzymes. In addition, MBP-fused enzymes showed remarkable folding ability in-vitro. Our findings, therefore, suggest that a previously unrecognized trait of this family, namely the ability to functional fusion with some other protein but yet to retain innate properties, is conserved. We described here the structural and evolutionary implications of the properties in this family enzyme.

• Korean Journal of Adult Nursing
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• v.14 no.1
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• pp.125-134
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• 2002

Purpose: The objectives for this study were to identify the factors that correlate with appraisal of illness and to explore what variables are predictive of cancer patients primary caregivers' cognitive appraisal for stress. Method: The subjects were selected by convenient sampling and 130 caregivers who completed a questionnaire. Measures used in this study included the Family Inventory of Resources for Management, Social Support Index, Family Crisis Oriented Personal Evaluation Scales and Family Coping Coherence Index. Pearson correlation was used to identify the relationship among factors and multiple regression was used to determine the individual and cumulative effect of potential predictors on the caregivers' appraisal. Results: Patient's level of activity, severity of the disease, quality of relation between patient and caregiver, caregiver's subjective health status, economic status, family resources and coping were significantly correlated. Among the variables, coping, family resources, economic status and quality of relation between caregiver and patient predicted 49.2 percent of the variance in appraisal of caregivers' stress condition. Conclusion: These findings suggest that coping mechanisms and family resources are important for positive appraisal. Nurses should provide adequate nursing care for the primary caregiver about professional care information and supportive counseling.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.2
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• pp.401-406
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• 2015

Background: This study was planned as comparative and descriptive in order to measure and evaluate the knowledge and attitudes regarding early diagnosis of women with and without a family history of cervical cancer. Materials and Methods: The study sample consisted of the relatives of female patients (N=253) who were admitted to Istanbul University of Medicine. Women with a family history of cervical cancer formed the case group, while those without family history of cervical cancer constituted the control group. Two distinct data collection tools, a questionnaire and the Miller Behavioral Style Scale (MBSS), were used in order to obtain data for evaluation with SPSS for Windows 20.0 statistics package program. Results: It was found that 61.0% of the case group with family history of cervical cancer and 19.0% of the control group without family history of cervical cancer were using early diagnostic methods. Thus the presence of an individual with cervical cancer in the family affected the attitudes towards early diagnosis. It was further found that the level of knowledge on cervical cancer and PAP smear test was higher in the case group, which was more sensitive with regard to being informed about cervical cancer as compared to general society. However, the average MBSS scores were not significantly different compared to the control group. Conclusions: It was noted that, women participating this study knowledgeable, but this did not necessarily transform into better behavior.

• Journal of Family Resource Management and Policy Review
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• v.12 no.2
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• pp.173-190
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• 2008

The purpose of this study is to develop and evaluate the effectiveness of the pre-parent education program and its application to adolescents in residential care. The following research question will be used in the evaluation of the program's effectiveness: a change in self-esteem of the adolescents in residential care and a change in defining an ideal family. With these research questions, a survey of the requirements for its contents and previous studies were researched, and adolescents in residential care participated in the program in 12 sessions of 4 stages. The research showed a meaningful change of p>.001 for self-esteem and the ideal family in pre, post-test to the experimental group and control group. That proved the effectiveness of the pre-parent education program. Observing the experimental group's change process, the researcher proved the following results. At the beginning of the program experimental group showed hopelessness and disliked participating in the program. At the end of this, they had active attitudes, confidence and challenge to the vision. This study contains theory and various practical family activities. This study made a contribution to help adolescents in residential care enhance self-esteem and define the future ideal family.

• Research in Community and Public Health Nursing
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• v.24 no.4
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• pp.480-487
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• 2013

Purpose: The purpose of this study was to investigate the relationship among family functioning, empathy and aggression by high school students. Methods: This was a descriptive study. The survey participants were 467 high school students from M city. Data were collected from November 29 to December 7, 2012 and self-report questionnaires including a Family Adaptability Cohesion Evaluation Scale, Interpersonal Reactivity Indexes, and an Aggression Questionnaire. The collected data were analyzed by descriptive statistics, independent-samples t-test, ANOVA, stepwise multiple regression. Results: The majority of the subjects were 2nd graders (42.0%) and female (57.2%). 59.7% of the subjects had religion, 84.8% were living together with parents. The mean ages of their fathers and mothers were $49.90{\pm}0.20$ and $46.85{\pm}0.19$ respectively. Aggression was found to have a statistically negative correlation with family functioning and empathy. Stepwise multiple regression analysis revealed that the most powerful predictor of aggression was empathic concern. Empathic concern, family cohesiveness, perspective taking, personal distress and gender accounted for 16.7% of the variances. Conclusion: It is necessary to provide aggressiveness reduction programs for high school students that consider their family functioning and empathy.

• Journal of Korean Society for Quality Management
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• v.30 no.2
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• pp.1-10
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• 2002

A performance evaluation model of an R&D module in the quality management system for M Company Is constructed using QFD. The company produces telecommunication devices and employs the performance evaluation model based on ISO 9000 family. However, the model has some problems, one of them is that all check items are regarded as equally important Customer requirements are gathered from internal customers and QFD is utilized to obtain weights for each check item.

• Proceedings of KHEA Conference
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• 1997.07a
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• pp.104-104
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• 1997

• Korean Journal of Family Social Work
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• no.23
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• pp.103-152
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• 2008

The purpose of this study was to develop a program for couples in early stage of marriage for enhancing family resilience based on the concept of 'Family Resilience' by Walsh. Based on the literature review of couples in early stage of marriage, marital enrichment, and Family Resilience, a program focused on belief system, organizational pattern, and communication process was designed and implemented. The program was applied to seven couples in early stage of marriage for four (3hours/session/week) sessions. The Wilcoxon Signed Ranks Test was used to compare the pre and post effects of the program. By operating a focus group composed of the program participants, a qualitative analysis of the participant's evaluation was performed. The pre and post comparative analysis showed a significant enhancement in belief system, organizational pattern, communication process, and dyadic adjustment. The results of the qualitative study in the focus group also reinforced the effectiveness of the program. The evaluation showed that the 3 hour/ 4 session program was appropriate in terms program format. The significances of this study are the concretion of the concept of Family Resilience and providing practice tools for the daily practitioner.

• Korean Journal of Family Social Work
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• no.55
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• pp.59-90
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• 2017

The purpose of this study is to identify the overall effect of Family Hope Partner Project conducted by the Korean Association for Children with Leukemia and Cancer (KACLC) for pediatric cancer patients and their families and provide empirical basic data for the support of them. For this purpose, this study applied a qualitative research methodology as follows; 6 parents and 5 children who used the service for more than one year through the Family Hope Partner Project participated in the interviews. The interviews were conducted from June to July 2016, and the collected data were analyzed through the processes of qualitative analysis. The results of this study are as follows. Pediatric cancer patients and their families who were previously confronted with problems such as a 'barrier of severe pain' and 'swirling anxiety and exhaustion' experienced multi-dimensional effects, thanks to the intervention of 'a genuine master serving as a stepping stone toward their recovery and growth', who were linked to the pediatric cancer patients through Family Hope Partner Project. In other words, they experienced positive effects such as 'support for overall rehabilitation of pediatric patients', 'improvement of family relations', 'relief of needs for social relations', and 'liberation from pressure'. The results of this study were meaningful, in that it substantially corroborated the multidimensional effects of the Family Hope Partner Project, and presented the development direction of the Family Hope Partner Project, which was the first of its kind at home and abroad initiated by the Korean Association for Children with Leukemia and Cancer (KACLC) for pediatric cancer patients and their families.

• Journal of Korean Academy of Nursing
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• v.36 no.6
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• pp.1042-1054
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• 2006

Purpose: Previous evaluation studies of the visiting nursing program explained an average change of the participants' health status, without considering socio-ecological characteristics and their impacts. However, these factors must affect individual health problems and lifestyles. For effective and appropriate community based programs, the Geographical Information System(GIS) can be utilized. GIS is a computer-based tool for mapping and analyzing things that happen on earth, and integrates statistical analysis with unique visualization. The purpose of this study was to evaluate visiting nursing care and to advocate the usefulness of planning and evaluating visiting nursing programs using Exploratory Spatial Data Analysis(ESDA) with GIS technology. Methods: One hundred eighty-four elderly participants with cerebrovascular risk factors who lived in 13 areas of one community received visiting nursing care. The data analyzed characteristics of pre-post change and autocorrelation by ESDA using GIS technology. Results: Visiting nursing care showed an improvement in the participants' lifestyle habits, and family management ability and stress level, while the improvements were different depending on the regions. The change of family management ability and stress level correlated with neighborhoods (Morgan's I=0.1841, 0.1675). Conclusions: Community health providers need to consider the individual participant's health status as well as socio-ecological factors. Analysis using GIS technology will contribute to the effective monitoring, evaluation and design of a visiting nursing program.