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Structure of Oocyte Surface in Two Korean Minnow Species, Rhynchocypris kumgangensis and R. oxycephalus (Pisces: Cyprinidae) (금강모치와 버들치 난모세포의 표피 구조)

  • Gwak, Jin-Young;Park, Jong-Young
    • Korean Journal of Ichthyology
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    • v.19 no.1
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    • pp.16-23
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    • 2007
  • Oocyte surface in two Korean minnows, Rhynchocypris oxycephalus and R. kumgangensis was examined by light and electron microscope. In two species, the development of the oocyte was similar, but the follicular layer surrounding full-grown oocyte showed an evident difference. In R. oxycephalus, the follicular layer at the yolk vesicle stage became bilaminar with the retention of its outer squamous cell layer and the acquisition of an inner cuboidal or round cell layer just over the zona radiata. As the oocyte grows, the cuboidal cells of the inner follicular layer began to be replaced by columnar cells. At the yolk granule stage, the columnar cells secreted mucin to their cytoplasm (adhesive materials) and then surround the entire oocyte, as bundles of fence-shaped structures. Whereas, although the follicular layer of R. kumgangensis had an outer squamous layer and an inner cuboidal or round cell layer at the yolk vesicles as in R. oxycephalus, no inner cells were more changed with the retention of its cuboidal or round cells. Finally, in R. kumgangensis, the adhesive materials did not occur. In Korean two minnows, the structural difference in the oocyte surface seems to be related to their habitats and spawning characteristics as well as taxonomic characters.

Youth Unemployment and Labor Policy in Contemporary Japan (일본 노동시장의 변화와 정책대응)

  • Hiroo, Kamiya
    • Journal of the Economic Geographical Society of Korea
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    • v.9 no.3
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    • pp.396-409
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    • 2006
  • In this paper, first I try to sketch the changing Japanese labor market after 1990s, and then examine the so-called "Job $Caf{\acute{e}}$ program" in detail, which was initiated in 2004 by central and local governments to help young people fine suitable jobs, and finally investigate the possible direction for future labor policy as an important tool for revitalize the local economy. Latter half of 1990s witnessed the high unemployment rate among the population aged twenties in Japan, and the number of 'shinsotsu-mugyo', i.e. population not at work after completing high school or university, NEET (not in employment, education or training) and 'freeters' have grown rapidly. "Job $Caf{\acute{e}}$ program" was initiated as a public response to the increased youth unemployment, aiming at assisting young people's transition from education to career. In the Job $Caf{\acute{e}}$ program, job information service by MHLW, information service for high school students and university students by MEXT, placement service of career internship by METI are integrated into one service, and are provided by local government initiative, therefore named as "one stop service of employment". Although it is highly appreciated for one stop service, the Job cafe program has criticized for paying too much attention to the performance of projects, such as the number of users, and the number of successful job matching. At the final section of the paper, more practical spatial unit for executing effective regional plan on local employment and more empirical research on job search behavior are discussed.

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A Study on Application of Yard Transportaion Equipment Automation System in the Container Terminal (컨테이너터미널에서 야드 이송장비 자동화 적용방안에 관한 연구)

  • Cha, Sang-Hyun;Noh, Chang-Kyun
    • Journal of Navigation and Port Research
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    • v.42 no.3
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    • pp.217-226
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    • 2018
  • International major container terminals are trying to expand automation facilities in order to dominate the competition and grow as central harbors. Recently, system design and development were put underway to fully integrate automation systems in container terminals. This is because it can focus on improving the efficiency of the yard operations in container terminals and facilitates integration with other container terminal operation processes. Automation in the container terminal business today is essential to better serve customers and improve profitability. Real-time position and status of container handling equipment (CHE) have been required for more efficient terminal operations and consequently higher productivity. This study examined the real-time location and status of the container terminal automation equipment using DGPS in container terminals, and reviewed the components and functions of the container terminal automation yard equipment systems that developed, applied, and operated the automation systems. In addition, this study compared the existing yard system and the automation yard system and presented application methods. Also, the results revealed that the productivity of the container terminal and the efficiency of the transportation equipment can be further increased when the optimal yard automation transportation equipment system is used to efficiently operate the transportation equipment, which is the core equipment of the container terminal.

Effects of Bisphenol A on Vitellogenin Synthesis in the Hepatocyte Primary Culture in the Olive Flounder, Paralichthys olivaceus (넙치 Paralichthys olivaceus 초대 배양 간세포의 Vitellogenin합성에 미치는 Bisphenol A의 영향)

  • Yeo, In-Kyu;Choe, Mi-Kyung
    • Korean Journal of Ichthyology
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    • v.12 no.3
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    • pp.180-185
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    • 2000
  • The effects of bisphenol-A(BPA), a monomer of plastics used in many consumer products, on vitellogenin(VTG) synthesis were examined in primary hepatocyte culture of olive flounder, Paralichthys olivaceus. The hepatocytes were precultured for 2 days, and then estradiol-$17\beta(10^{-6}M)$ and BPA were simultaneously added to the incubation medium. The hepatocytes were cultured for 6 more days and then spent medium was analyzed by SDS-PAGE. BPA increased the rate of VTG to total protein concentrations in a concentration-dependent way, and a significant difference was obtained at concentrations of $10^{-6}M$ and $10^{-5}M$ (P<0.05). In particular, the rate of VTG to total protein concentrations was 26.36% at $10^{-5}M$ of BPA, and its level did not differ from the control level with $E_2$ alone. $E_2$ and/or BPA-primed VTG synthesis was markedly inhibited to about 80% of the control(with $E_2$) by the addition of tamoxifen($10^{-6}M$) to the incubation medium. Furthermore, In vivo $E_2$-primed VTG synthesis was significantly inhibited by in vitro $E_2$-free incubation of hepatocyte to about 22% of the control (with $E_2$) on Day 6. The effect of reducing was delayed in a BPA concentration-dependent way. These results suggest that BPA induce VTG synthesis by estrogenic activity through estrogen receptor mediated response and prolong VTG synthesis on vitellogenesis in olive flounder.

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Temperature-dependent Fecundity and Life Table Parameters of Aphis gossypii Glover (Homoptera: Aphididae) on Cucumber Plants (오이에서 온도에 따른 목화진딧물 산자수 및 생명표)

  • Kim Ji-Soo;Kim Tae-Heung
    • Korean journal of applied entomology
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    • v.43 no.3 s.136
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    • pp.211-215
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    • 2004
  • Temperature-fecundity of the melon aphid, Aphis gossypii Glover, was studied at constant temperatures ranging from 15 to $32.5^{\circ}C$ under $60-70\%$ RH and a photoperiod of 16 : 8 (L : D) A life table parameters were constructed using the results. The longevity of A. gossypii gradually increased with decreasing temperature below $27.5^{\circ}C$. Also fecundity increased with decreasing temperature and the highest fecundity was 61.8 nymphs per female at $17.5^{\circ}C$. However. daily fecundity increased with increasing temperatures up to $22.5^{\circ}C$ showing 5.9 nymphs per day and thereafter decreased. Longevity and fecundity of the adult in the greenhouse with an average temperature of $21^{\circ}C$ and $65.6\%$ RH, were 20.0 days and 59.6, respectively, which were longer and higher than those in the growth chamber with similar conditions. net reproductive rate (Ro) was 54.9 at $17.5^{\circ}C$ while intrinsic rate of increase ($r_m$) and finite rate of increase ($\lambda$) were the highest 0.5 and 1.6 at $30^{\circ}C$, respectively. doubling time (DT) and mean generation time (T) were the shortest 1.4 and 6.8 at $30^{\circ}C$ indicating that optimal temperature for the development is $30^{\circ}C$.

Effects of Greenhouse Covering Material on Environment Factors and Fruit Yield in Protected Cultivation of Sweet Pepper (파프리카 재배 온실의 피복재 종류에 따른 환경요인과 수량성)

  • Kim, Ho-Cheol;Jung, Sek-Gi;Lee, Jeong-Hyun;Bae, Hyang-Jong
    • Journal of Bio-Environment Control
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    • v.18 no.3
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    • pp.253-257
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    • 2009
  • To analysis effect of environment factors on productivity of sweet pepper according to greenhouse covering material (glass, plastic film), this was investigated. In glasshouse, outside light was positively correlated with yield as that $100MJ{\cdot}m^{-2}$ of outside light increased $300{\sim}500g{\cdot}m^{-2}$, also cumulative temperature was same tendency. On possibility of model development for yield estimate cumulative temperature was high than outside light. According to covering material, leaf photosynthesis, productivity per out-side light and term in glasshouse was more high 13%, 46%, and 47% compared with plastic film house, respectively. Result of analysis of effect of light, temperature, and $CO_2$ on yield, relative yield coefficient, yield increment coefficient, and yield reduction coefficient in glasshouse were more high 25%, 73%, and 34% compared with plastic film house, respectively. Hence, sweet pepper's growing in glasshouse compare with plastic film house had more productivity, but that had more sensitivity to charge of environment factors.

Spatial Distribution Pattern of Ascotis selenaria (Lepidoptera: Geometridae) larvae in a Small-Scale of Citrus Orchard (소규모 감귤원에서 네눈쑥가지나방 유충의 공간분포 특성에 대한 이해)

  • Choi, Kyung San;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.52 no.3
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    • pp.243-248
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    • 2013
  • This study was conducted to understand the settlement process of Ascotis selenaria larvae into citrus orchards with respect to oviposition site and analysis of the spatial distribution pattern of the larvae. A. selenaria eggs were not found on citrus trees in field and green house, but not on citrus trees in the field. A. selenaria larvae showed a significant clump distribution in the greenhouse. In the open citrus field, the index of dispersion was around 1.0 in most cases, with a weak clumping degree. However, the d-statistic was between -1.96 and 1.96, indicating a statistically significant random distribution. In addition, the Green's index (a clumping index) was very low in all cases, even though the clump distribution was accepted. for most samples, the probability distribution of larval frequency in the field satisfied the probability distribution functions of Poisson (random pattern) and the negative binomial (clump pattern) distribution. In addition, the temporal distribution of the larvae in the open field showed a pattern which was formed by colonizers from outside oviposition sites. Further, the difference in larval spatial distribution between field and greenhouse orchards was discussed.

Comparison of General Test Methods of Various Organs on Synthetic Food Colors (여러 기관의 일반시험법에 의한 식용 타르색소의 규격 비교 시험)

  • Shin Dong-Hwa;Kim Yong-Suk;Lee Young-Hwan;Bang Jeong-Ho;Om Ae-Son;Shin Jae-Wook;Lee Tal-Soo;Hong Ki-Hyoung;Park Sung-Kwan;Choi Jang-Duck;Kim Hee-Yun
    • Journal of Food Hygiene and Safety
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    • v.19 no.4
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    • pp.171-175
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    • 2004
  • An analytical method of nine synthetic food colors in Korea, Japan, Joint FAO/WHO Expert Committee of Food Additives (JECFA), and USA were compared. Contents of water insoluble matter in synthetic food colors tested were not different by general test methods of various organs. Contents of chloride and sulfate salts in Food Green No.3, Food Red No.3, Food Blue No.2, and Food Yellow No.4 slightly differed in various methods, and but up to the standard, and general test methods of JECFA and USA needed far more time. For the measurement of arsenic contents in food colors, colorimetric method in Korea and Japan, and silver diethyldithiocarbamate colorimetric method in USA and JECFA were used, but the standards of them were different. Content of heavy metals was up to the standard, but the methods were used colorimetric method in Korea, atomic absorption method in Japan, and both methods in JECFA and USA.

Studies on the Determination of Synthetic Food Colors (합성식용색소의 정량분석법에 관한 검토)

  • Om Ae-Son;Lee Heon-Ok;Shim Jae-Young;Shin Dong-Hwa;Kim Yong-Suk;Lee Young-Hwan;Bang Jeong-Ho;Shin Jae-Wook;Lee Tal-Soo;Hong Ki-Hyoung;Park Sung-Kwan;Choi Duck-Jang;Kim Hee-Yun
    • Journal of Food Hygiene and Safety
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    • v.20 no.2
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    • pp.73-76
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    • 2005
  • Center for Food Standard Evaluation, Korea Food and Drug AdministrationThis study was performed to compare analytical methods of nine synthetic food colors and six food color lakes in Korea, Japan, Joint FAO/WHO Expert Committee of Food Additives (JECFA), and USA. The experimental protocol of this study consists of three parts: titration method with titanium chloride, gravimetric and spectrophotometric method. To measure the total contents of food colors, Korea and Japan used titration method with titanium chloride, USA used the average value of titration method with titanium chloride equipped with Kipp generator and spectrophotometric method. Also, JECFA used titration method with titanium chloride equipped with KiPP generator. However, All the low organizations used gravimetric method to measure the total content of coloring matter on Food Red No.3. Although all organizations use various methods for analysis of coloring matters, total contents of coloring matter on food colors tested fell into the standard showing $85.08-96.40\%$ in synthetic food colors and $10.00-36.86\%$ in food color lakes.

THE ANTIMICROBIAL EFFECT OF HORSERADISH(ARMORACIA RUSTICANA) ROOT EXTRACTS AGAINST STREPTOCOCCUS MUTANS ISOLATED FROM HUMAN DENTAL PLAQUE (치태에서 분리된 Streptococcus mutans에 대한 서양산 고추냉이(Armoracia rusticana) 뿌리 추출물의 항균효과)

  • Kim, Hye-Kyoung;Park, Ho-Won;Shin, Il-Shik;Lee, Ju-Hyun;Seo, Hyun-Woo
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.2
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    • pp.225-234
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    • 2008
  • Recently interesting in development of antimicrobial agent from natural origin has been increased in these days. Many studies have been reported antimicrobial effect of Horseradish(Armoracia rusticana) root extracts against various microorganisms such as Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Vibrio parahaemolyticu, Staphylococcus aureus, Bacillus subtilis, Aspergillus parasiticus, Helicobacter pylori. The main component related to antimicrobial activity in horseradish is well known as allyl isothiocyanate(AIT). In this study, we investigated the antimicrobial effects of Horseradish(Armoracia rusticana) root extracts against Streptococcus mutans isolated from human dental plaque, Streptococcus mutans reference strain and compared with that of chlorhexidine. Horseradish root extracts and chlorhexidine were tested to determine their minimum inhibitory concentration( MIC) and minimum bactericidal concentration(MBC). The result of this study can be summerized as follows; 1. Horseradish root extracts showed antimicrobial effect against both S. mutans isolated strain and reference strain, their MIC were respectively $0.083{\sim}0.25%$ ($833.33{\sim}2500 ppm$), 0.25% (2500 ppm). Horseradish root extracts showed antimicrobial effect against S. mutans isolated strain at same or slightly lower concentration compared with MIC of reference strain. 2. $0.083{\sim}0.25%$ horseradish root extracts showed similar antimicrobial effect with chlorhexidine ($0.0021{\sim}0.0041%$).

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