• Mycobiology
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• 제31권4호
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• pp.205-208
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• 2003

The production of polysaccharide according to various developmental stages(mycelium growth, primordium appearance, and fruiting-body formation) in the edible mushroom Grifola frondosa was studied. The cap of the mature mushroom showed the highest amount of polysacchride. Mycelial growth and polysaccharide synthesis were optimal at pH 5 and $20^{\circ}C$. Polysaccharide synthesis was maximal after 12 days of cultivation, whereas maximum mycelial growth was shown after 18 days. Mannose, cellobiose and starch increased the level of polysaccharide as well as growth in submerged culture. Glucose and sucrose appeared to be good substrates for fruiting of Grifola frondosa.

• Mycobiology
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• 제37권2호
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• pp.155-157
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• 2009

Thirty-seven single spore isolates were obtained from specimens of ascomycetous fruiting bodies collected from Mt. Suri, Anyang in Korea. The fungal specimens and isolates were identified as Dumontinia tuberosa based on their morphological and cultural characteristics. This is the first record of this fungus occurring in Korea.

• International Journal of Industrial Entomology and Biomaterials
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• 제18권1호
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• pp.13-17
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• 2009

The caterpillar-shaped Chinese medicinal mushroom (DongChongXiaCao) looks like a worm in the winter and like a grass in the summer. The fruiting body has been regarded as popular folk or effective medicines used to treat human diseases such as asthma, bronchial and lung inflammation, and kidney disease. The fruiting bodies of Cordyceps militaris, C. pruinosa and Paecilomyces tenuipes that formed on the living silkworm (Bombyx mori) host were used in this examination. This study was carried out to investigate the soluble sugar, amino acid and fatty acid profiles in the fruiting-bodies. Soluble sugars such as glycerol, glucose, mannitol and sucrose were mainly found in the fruiting bodies of C. militaris, C. pruinosa and P. tenuipes. Total soluble sugar content was 29.23 mg/g in C. militaris, 8.61 mg/g in C. pruinosa and 24.00 mg/g 1in P. tenuipes on dry weight basis. Total free amino acid content was 14.09 mg/g 1in C. militaris, 34.60 mg/g in C. pruinosa and 17.09 mg/g in P. tenuipes. The content of oleic acid in fatty acids was above high more than 30% regardless of species.

• 한국균학회지
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• 제36권1호
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• pp.98-100
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• 2008

자작나무버섯은 자작나무류 수종에 발생하는 버섯으로 상업적 가치가 있는 것으로 판단되나, 우리나라에서는 매우 드물게 발생하고 있어 물박달나무를 이용하여 원목재배를 시도하였다. 자작나무버섯은 4월에 균 접종 후 2년차의 8윌초에 버섯을 형성하여 10월에 성숙하였다. 버섯은 골목 1개당 1개 또는 2개 발생하여 생중량 $212{\sim}1,298\;g$을 수확하였다. 그래서 자작나무버섯의 원목재배는 자실체 대량 생산이 가능하며 버섯을 이용한 새로운 연구가 기대된다.

• 한국산림과학회지
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• 제98권6호
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• pp.676-680
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• 2009

저농도 SCB(Slurry Composting Biofiltration)액비 시비가 생장 및 결실특성에 미치는 영향을 알아보고자 접목 7년생 '축파(Tsukuba)' 품종을 대상으로 SCB액비, 화학비료, 유기질비료, 무처리구 등 4처리를 관행 시비량에 준한 시비 처리로 2008년부터 2009년까지 2년간 생장 및 결실특성을 각각 조사하였다. 수고 및 근부직경의 생장은 전년도에 비해 화학비료와 SCB액비 처리구에서 각각 8.18%와 26.93%, 8.04%와 25.90%의 증가량을 보였으며, 착구부 직경에 대한 기부직경의 비는 화학비료 처리구를 제외하고 전년도에 비해 모든 처리구에서 감소되었다. SCB액비 처리구에서 착과율이 86.25%로 높게 나타남에 따라 SCB액비가 생장과 수세에 기여하는 양료 역할을 하는 것으로 판단되며 새로운 비종으로서 대체 효과가 있는 것으로 나타났다.

• Mycobiology
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• 제35권4호
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• pp.215-218
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• 2007

The caterpillar-shaped Chinese medicinal mushroom (DongChongXiaCao) looks like a worm in the winter and like a grass in the summer. The fruiting body has been regarded as popular folk or effective medicines used to treat human diseases such as asthma, bronchial and lung inflammation, and kidney disease. The fruiting bodies of Paecilomyces tenuipes that formed on the living silkworm (Bombyx mon) host were used in this examination. This study was carried out to investigate the proximate composition, soluble sugar, amino acid and fatty acid profiles, and contents of the bioactive ingredient including adenosine and D-mannitol in the fruiting-bodies. The moisture content was 57.56%. Soluble sugars found were glycerol, glucose, mannitol and sucrose, and the contents exceeded $24\;mgg^{-1}dry$ weight. Total free amino acid content was $17.09\;mgg^{-1}dry$ weight. Arginine, glycine, proline and tyrosine were main amino acids. The content of oleic acid in fatty acids was high. Adenosine was more abundant in fruiting bodies than corpus.

• Mycobiology
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• 제37권1호
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• pp.10-16
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• 2009

The purpose of this study is to understand spatio-temporal changes of active fungal biomass and water in Tricholoma matsutake soil colonies during the mushroom fruiting season. The active fungal biomass was estimated by analyzing ergosterol content at four different points within four replicated locations in a single circular T. matsutake colony at Ssanggok valley in the Sogri Mt. National Park in Korea during 2003 to 2005. The four points were the ahead of the colony, the front edge of the colony and 20 cm and 40 cm back from the front edge of the colony. Ergosterol content was 0.0 to 0.7 ${\mu}g$ per gram dried soil at the ahead, 2.5 to 4.8 ${\mu}g$ at the front edge, 0.5 to 1.8 ${\mu}g$ at the 20 cm back and 0.3 to 0.8 ${\mu}g$ at the 40 cm back. The ergosterol content was very high at the front edge where the T. matsutake hyphae were most active. However, ergosterol content did not significantly change during the fruiting season, September to October. Soil water contents were lower at the front edge and 20 cm back from the front edge of the colony than at the ahead and 40 cm back during the fruiting season. Soil water content ranged from 12 to 19% at the ahead, 10 to 11% at the edge, 9 to 11% at the 20 cm back and 11 to 15% at the 40 cm back. Our results suggest that the active front edge of the T. matsutake soil colony could be managed in terms of water relation and T. matsutake ectomycorrhizal root development.

• 한국버섯학회지
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• 제18권1호
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• pp.84-90
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• 2020

국내의 다양한 지역에서 수집한 19 야생버섯균주를 이용하여 배지조성인 참나무 톱밥과 밀기울을 부피비율 7:3으로 병재배 650 g/1,100 ml에서 자실체를 생산하였다, 시험한 19균주중 14균주가 원기를 형성하였으며 자실체를 생산 할 수 있었으며 대조품종 노루1, 2호의 병당 75 g보다 KFRI349, KFRI1091, KFRI1093은 병당 120 g 이상으로 높은 수확량을 나타내었다. 자실체 색형은 백색과 핑크색으로 나눌 수 있었으며 침의 형태에 따라 고드름형과 산호형으로 구분할 수 있었다. 전자현미경(SEM)에 의한 노루궁뎅이버섯의 담자기, 포자, 침의 형태를 포함한 자실체 미세구조를 관찰 하였으며 UFP-PCR분석으로 균주간의 PCR다형성밴드가 검출되어 4 그룹으로 분류 할 수 있었으며 높은 유전적 다양성을 확인 할 수 있었다.

• 한국버섯학회지
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• 제17권4호
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• pp.275-283
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• 2019

Sparassis latifolia is a fungus abundant in β-glucan and amino acids and is highly valued as a medicinal mushroom. Among amino acids, γ-aminobutyric acid (GABA) is a free amino acid and has biological effects, such as increase/decrease of hypertension, improvement of cerebral blood flow, and prevention of dementia. In this study, biological elicitors were used to increase bioactive substances as a biofortification method. Sodium alginate extracted from seaweed (Sargassum horneri, Sargassum fulvellum, Sargassum fusiforme) were used as the elicitor. The levels of β-glucan and GABA in the mycelium and fruiting body grown by adding the elicitor to the medium were investigated. Addition of sodium alginate positively affected GABA production and negatively affected the β-glucan production in these fungi. Sodium alginates extracted from S. fulvellum induced the highest increase in GABA in the mycelium and fruiting bodies. Moreover, we investigated the effects of the extracts from mycelium and fruiting bodies on dendrite development in primary cortical neurons. We found that the extract from the fruiting bodies of sodium alginate treated fungi with increased levels of GABA inhibited the dendrite outgrowth of excitatory neurons, but not inhibitory neurons.

• 미생물학회지
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• 제37권4호
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• pp.239-244
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• 2001

충남 대둔산의 토양으로부터 분리한 점액세균을 ARJ라 명명하고 그 특성을 알아보았다. CT 배지에서 swarming을 하며 성장한 이 균주는 slime을 형성하였고, 액체 및 고체배지 상에서 황록색의 색소를 방출하였으며 swarming 부분을 파장이 366 nm인 자외선으로 조사하였을 때 초록색의 형광을 띄었다. 또한 이 균주는 영양분이 고갈되었을 때 fruiting body를 형성하는 것으로 보아 Myxobacteria라고 생각되었으며 주사전자 현미경 관찰 결과 이 균주가 형성한 fruiting body는 stalk이 없었고 naked myxospore를 가지고 있었다. Myxobacteria의 분류에 있어서 가장 중요한 이러한 형태학적 특성들로 볼때 이 균주는 Myxococcus virescens와 가장 유사한 것으로 밝혀졌다. 한편 이 균주는 특히 gram 양성 세균에 대해 antimicrobial activity가 있는 것으로 나타났는데, 여과한 배양 여액에서도 이렇ㄴ activity가 있었지만 비변성 polyacrylamide 전기영동을 하여 본 결과 이 물질이 단백질은 아닌 것으로 밝혀졌다. 또한 이 배양 여액은 상당한 proteolytic activity가 있었으며 최소한 2개의 proteolytic enzyme이 있는 것으로 비변성 polyacrylamide 전기영동을 통하여 밝혀졌다.