Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Enhanced GABA content from sodium alginate-induced Sparassis latifolia influences dendrite development in primary cortical neurons

해조류 기반 엘리시터 처리에 의한 꽃송이버섯의 GABA 함량 증가 및 흥분성 신경세포의 수상돌기 발달 억제

  • Choi, Moon-Hee (The Research Institute of Advanced Engineering Technology, Chosun University) ;
  • Ki, SungHwan (College of Pharmacy, Chosun University) ;
  • Lee, Seong-Eun (College of Pharmacy, Chosun University) ;
  • Lee, GumHwa (College of Pharmacy, Chosun University) ;
  • Shin, Hyun-Jae (Department of Biochemical Polymer Engineering of Chosun University)
  • 최문희 (조선대학교 공학기술연구원) ;
  • 기성환 (조선대학교 약학대학) ;
  • 이성은 (조선대학교 약학대학) ;
  • 이금화 (조선대학교 약학대학) ;
  • 신현재 (조선대학교 생명화학고분자공학과)
  • Received : 2019.12.17
  • Accepted : 2019.12.20
  • Published : 2019.12.31
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Abstract

Sparassis latifolia is a fungus abundant in β-glucan and amino acids and is highly valued as a medicinal mushroom. Among amino acids, γ-aminobutyric acid (GABA) is a free amino acid and has biological effects, such as increase/decrease of hypertension, improvement of cerebral blood flow, and prevention of dementia. In this study, biological elicitors were used to increase bioactive substances as a biofortification method. Sodium alginate extracted from seaweed (Sargassum horneri, Sargassum fulvellum, Sargassum fusiforme) were used as the elicitor. The levels of β-glucan and GABA in the mycelium and fruiting body grown by adding the elicitor to the medium were investigated. Addition of sodium alginate positively affected GABA production and negatively affected the β-glucan production in these fungi. Sodium alginates extracted from S. fulvellum induced the highest increase in GABA in the mycelium and fruiting bodies. Moreover, we investigated the effects of the extracts from mycelium and fruiting bodies on dendrite development in primary cortical neurons. We found that the extract from the fruiting bodies of sodium alginate treated fungi with increased levels of GABA inhibited the dendrite outgrowth of excitatory neurons, but not inhibitory neurons.

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References

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