• 한국식품과학회지
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• 제38권2호
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• pp.284-289
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• 2006

최근 건강에 대한 관심이 증가하고 유리기 산소에 의한 산화 및 염증이 다양한 질환과 관련이 있다는 사실이 밝혀지면서 식품류는 의약품에 비해 장기간 섭취하여도 안전하고 친숙하게 접할 수 있는 장점 때문에 천연물이나 각종 식품류에서 항산화 및 항염증과 관련된 새로운 물질을 탐색하는 연구가 활발하게 진행중에 있다. 그 가운데 4배성 휘커스 오디품종 '대성뽕' [Morus Lhos(Ser.) Koids]의 주색소는 anthocyanins이며, cyanidin-3-glucoside형태로 0.794% DW가 함유되어 있어서, 다른 오디품종에 비해 많은 양이므로 항산화 활성도 비례하여 클 것으로 판단된다. 따라서 본 연구에서는 오디추출물의 중년 남, 녀의 혈청 무기질과 항산화 관련 물질에 대한 개선에 대한 효과를 알아보고자 4주간 오디추출물을 급여(100 mL/day)하여 섭취 전 후의 혈청 무기질 및 혈중 항산화 관련물질을 분석하여 비교하였다. 1차적으로는 오디추출물을 제조하여 무기질 함량과 항산화능을 측정한 결과 무기질 함량은 칼슘(80.66 mg), 마그네슘(12.26 mg), 철분(6.26 mg), 구리(0.05 mg), 아연(4.01 mg) 함량으로 나타나 칼슘, 철분, 아연 함량이 높게 나타났다. DPPH소거능에 따른 오디추출물의 항산화능을 보면 비타민 C를 기준(100%)으로 했을때 cyanidin-3-glucoside는 85%,오디추출물은 34%로 cyanidin 3-glucoside의 약 40%의 소거능력을 보였다. 2차적으로는 이러한 오디추출물 섭취 전과 후 대상자들의 혈청 중 무기질 수준을 조사해 보았을때 여자 대상자의 경우 혈청 철분, 구리 및 아연 함량이 유의적으로 증가되었고, 남자 대상자의 경우는 철분 함량만이 유의적인 증가를 보였다. 혈청 지질산화와 관련 있는 혈청의 TBARS와 FRAP수준의 변화를 조사한결과 오디추출물 급여 후 남자, 여자 대상자의 혈청 FRAP수준이 유의적으로 증가되었고, 평균 혈청 TBARS 수준은 남, 녀 모두 감소되는 경향은 보였다. 결과적으로 오디는 항산화성이 뛰어나고 무기질을 공급할 수 있는 새로운 과일로 가치가 있다고 판단된다.

• 농업생명과학연구
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• 제44권6호
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• pp.133-140
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• 2010

초고압 처리가 홍삼의 진세노사이드 함량 및 항산화 활성에 미치는 영향에 대하여 조사하였다. 전통적인 방법으로 제조한 일반 홍삼과 초고압 처리 홍삼의 조사포닌 함량은 각각 21.93 및 27.29 mg/g이었고, 총 페놀성 화합물과 조사포닌 및 진세노사이드 함량은 초고압 처리에 의해 증가하였다. 일반 홍삼과 초고압 처리 홍삼에 가장 많이 함유되어 있는 진세노사이드는 Rb1으로 각각 14.10 mg/g과 17.71 mg/g 이었으며, 초고압 처리에 의하여 25.60% Rb1이 증가하였다. 또한 홍삼의 특징적인 유효 성분인 Rg3는 일반 홍삼에 비하여 초고압 처리한 홍삼에서 10.46% 증가하였다. DPPH 및 ABTS 라디칼 소거 활성을 측정한 결과, 열수 추출물의 농도가 증가함에 따라 DPPH 및 ABTS 라디칼 소거 활성이 증가하는 경향을 보였으며 초고압 처리 홍삼이 일반 홍삼 열수 추출물에 비해 높은 라디칼 소거 활성을 보였다. 환원력과 FRAP 활성도 분획물의 농도가 증가함에 따라 환원력과 FRAP 활성이 증가하는 경향을 보였으며, 일반 홍삼과 초고압 처리 홍삼 열수 추출물의 FRAP 활성은 농도 10 mg/mL일 때 각각 0.30 및 0.36의 흡광도를 보였다. 따라서 초고압 처리 홍삼 열수 추출물은 자유 라디칼에 의해 유발된 산화적 스트레스를 효과적으로 방어할 수 있을 것으로 판단된다.

• 한국응용곤충학회지
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• 제61권4호
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• pp.669-673
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• 2022

국내 생산 감로꿀의 기능성 식품 소재로 활용하기 위하여 DPPH (1,1-diphenyl-2-picryl hydrazyl)와 ABTS [2,2-azobis (3-ethylbenzothiazoline-6-sulfonate)] 라디칼 소거능, FRAP (Ferric reducing antioxidant power) 활성, 총 폴리페놀 함량 분석을 통하여 항산화 활성을 평가하였다. 국내 생산 감로꿀은 DPPH와 ABTS 라디칼 소거 활성을 보였으며 최고농도 10 mg/mL에서 DPPH에 비하여 ABTS 라디칼 소거능이 3배 이상 높게 측정되었다. FRAP 분석에서는 ferric ion (Fe³⁺)를 126 µM의 ferrous (Fe²⁺)로 환원시켰다. 또한, 국내 생산 감로꿀에 함유된 폴리페놀성 화합물의 총량은 73.41 mg GAE/kg로 확인되었다. 이상의 연구결과에 비추어 볼 때, 국내 생산 감로꿀의 우수한 라디칼 소거능과 환원력을 가지며 천연 항산화제로 알려진 페놀성 화합물을 존재로 인하여 항산화 활성을 가지는 것으로 확인되었다. 본 연구결과는 국내 생산 감로꿀의 기능성 식품 소재로 활용할 수 있는 기초자료로 제공하는 바이다.

• 동의생리병리학회지
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• 제19권1호
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• pp.179-183
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• 2005

Recent interest in the possible protective effects of dietary antioxidant compounds against human degenerative disease has prompted investigation of foods such as blueberries, which have a high antioxidant capacity. This study was performed to determine the antioxidative activity of methanol extract and solvent fractions from blueberry. Blueberry was extracted with methanol and then fractionated with n-hexane, EtOAc, BuOH and water to get active fractions. And their antioxidant capacities in each fraction were determined by using the DPPH and FRAP assay, and tyrosinase inhibitor. Ethyl acetate fraction of blueberry exhibited antioxidant capacity.

• Current Research on Agriculture and Life Sciences
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• 제28권
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• pp.25-29
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• 2010

The colored sweet potato, particularly purple sweet potato, has been well known to contain anthocyanins abundantly. This study was conducted to examine the antioxidant properties of purple sweet potato. The chopped purple sweet potato was extracted 2 times with water or acetone for 18 hours at $28^{\circ}C$. The antioxidative potential of each solvent extract was assessed by DPPH free radical scavenging activity assay, FRAP assay, and total phenolic contents. The results showed that both extracts had not only high DPPH free radical scavenging activity but had high level of total phenolic compounds. Furthermore, both solvent extracts were found to have antioxidative effects in human colon cancer cells (HCT 116, HT 29) in DCFDA assay. The notable antioxidant activity of purple sweet potato suggests its significant health benefit and deserves further study to develop into functional food ingredient.

• Molecular & Cellular Toxicology
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• 제5권3호
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• pp.237-242
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• 2009

The potential antioxidant activities of different fractions from methanolic extract of Curcuma longa L. were assayed in vitro. All of the fractions exception of n-hexane and $H_2O$ showed a strong antioxidant activity, especially the ethylacetate (EtOAc) fraction, which showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity ($IC_{50}=9.86\;{\mu}g/mL$). The results of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay showed concentration dependency, the EtOAc fraction demonstrating a better result than the other fractions at the same concentration in this studies. Additionally, when the total phenolic contents of fractions were measured, the EtOAc fraction contained the highest level. Meanwhile, correlation analysis indicated a high correlation between the antiradical activity and the total phenolic contents, suggesting that fractions obtained from the methanolic extract of Curcuma longa L. have wide potential for use as sources of antioxidant material.

• Food Science and Biotechnology
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• 제17권6호
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• pp.1310-1315
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• 2008

This study was to investigate the influence of pH on the antioxidant activity of melanoidins formed from glucose (Glc) and fructose (Fru) with lysine enantiomers in the Maillard reaction. Melanoidins formed from D-isomers were found to be effective antioxidants in different in vitro assays with regard to the ferrous ion chelating activity, 1, l-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging activities, ferric reducing/antioxidant power (FRAP), and 2,2'-azinobis(3-ethylbenothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity. In particular, the chelating activity of these melanoidins at a pH of 7.0 was greater than those with pH of 4.0 and 10.0. The chelating activity and DPPH radical scavenging activity of the melanoidins formed from the Glc systems were higher than those of the melanoidins formed from the Fru systems. However, the FRAP and ABTS radical scavenging activity of these melanoidins were not different according to pH level, with exceptions being the Fru systems.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2001년도 10주년 기념 국제학술 심포지움 및 추계학술대회
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• pp.118-118
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• 2001

• Nutrition Research and Practice
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• 제2권3호
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• pp.158-164
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• 2008

This study investigated that the antioxidative effect of freeze-dried cranberry powder against protein and lipid oxidation and ameliorative effect of serum lipid profile in rat fed atherogenic diet. Six weeks old male Sprague-Dawley rats were divided into the following four groups: normal diet group with 5% com oil(control), atherogenic diet group with 5% com oil, 10% lard, 1% cholesterol, and 0.5% sodium cholate(HFC), atherogenic plus 2% cranberry powder diet group(HFC+C2), and atherogenic plus 5% cranberry powder diet group(HFC+C5), and respective diet and water were fed daily for 6 weeks. After the experimental period, the serum lipid profile, such as total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglyceride, ferric reducing ability of plasma(FRAP), plasma phenolics content, superoxide dismutase(SOD) activity, serum protein carbonyl and thiobarbituric acid reactive substances(TBARS) levels were examined. Total phenolic compound and total flavonoid levels in freeze-dried cranberry powder were 9.94 mg/g and 8.12 mg/g, respectively. Serum total cholesterol and LDL-cholesterol levels were not significantly different for cranberry powder treatment, but serum HDL-cholesterol level was significantly increased in HFC+C5 group compared with HFC group. Plasma FRAP value tended to be increased by cranberry powder treatment though there was no significant difference. Plasma total phenol concentrations and SOD activities were not significantly different among all groups. Serum protein carbonyl and TBARS levels were significantly decreased in HFC+C5 group compared with HFC group. Overall results suggested that freeze-dried cranberry powder might have the serum lipid improving effect, as well as anti oxidative effect demonstrated by its protective effect against protein and lipid oxidation.

• Nutrition Research and Practice
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• 제9권6호
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• pp.569-578
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• 2015

BACKGROUND/OBJECTIVES: Fermentation can increase functional compounds in fermented soybean products, thereby improving antioxidant and/or anti-inflammatory activities. We investigated the changes in the contents of phenolics and isoflavones, antioxidant activity and anti-inflammatory activity of Doenjang during fermentation and aging. MATERIALS/METHODS: Doenjang was made by inoculating Aspergillus oryzae and Bacillus licheniformis in soybeans, fermenting and aging for 1, 3, 6, 8, and 12 months (D1, D3, D6, D8, and D12). Doenjang was extracted using ethanol, and sequentially fractioned by hexane, dichloromethane (DM), ethylacetate (EA), n-butanol, and water. The contents of total phenolics, flavonoids and isoflavones, 2,2-diphenyl-1 picryl hydrazyl (DPPH) radical scavenging activity, and ferric reducing antioxidant power (FRAP) were measured. Anti-inflammatory effects in terms of nitric oxide (NO), prostaglandin (PG) E2 and pro-inflammatory cytokine production and inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expressions were also measured using LPS-treated RAW 264.7 macrophages. RESULTS: Total phenolic and flavonoid contents showed a gradual increase during fermentation and 6 months of aging and were sustained thereafter. DPPH radical scavenging activity and FRAP were increased by fermentation. FRAP was further increased by aging, but DPPH radical scavenging activity was not. Total isoflavone and glycoside contents decreased during fermentation and the aging process, while aglycone content and its proportion increased up to 3 or 6 months of aging and then showed a slow decrease. DM and EA fractions of Doenjang showed much higher total phenolic and flavonoid contents, and DPPH radical scavenging activity than the others. At $100{\mu}g/mL$, DM and EA fractions of D12 showed strongly suppressed NO production to 55.6% and 52.5% of control, respectively, and PGE2 production to 25.0% and 28.3% of control with inhibition of iNOS or COX-2 protein expression in macrophages. CONCLUSIONS: Twelve month-aged Doenjang has potent antioxidant and anti-inflammatory activities with high levels of phenolics and isoflavone aglycones, and can be used as a beneficial food for human health.