Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Antioxidative Activities of Solvent Extracts from Blueberry

Blueberry 추출물의 항산화 효과

  • Kim, Tae-Choon (College of Life Science and Natural Resources, Wonkwang University) ;
  • Bae, Kang-Soon (College of Life Science and Natural Resources, Wonkwang University) ;
  • Kim, Il-Kwang (Division of Natural Science, College of Natural Sciences, Wonkwang University) ;
  • Chun, Hyun-Ja (Division of Natural Science, College of Natural Sciences, Wonkwang University)
  • 김태춘 (원광대학교 생명자원과학대학) ;
  • 배강순 (원광대학교 생명자원과학대학) ;
  • 김일광 (원광대학교 자연과학대학 자연과학기술학부) ;
  • 천현자 (원광대학교 자연과학대학 자연과학기술학부)
  • Published : 2005.02.25
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Abstract

Recent interest in the possible protective effects of dietary antioxidant compounds against human degenerative disease has prompted investigation of foods such as blueberries, which have a high antioxidant capacity. This study was performed to determine the antioxidative activity of methanol extract and solvent fractions from blueberry. Blueberry was extracted with methanol and then fractionated with n-hexane, EtOAc, BuOH and water to get active fractions. And their antioxidant capacities in each fraction were determined by using the DPPH and FRAP assay, and tyrosinase inhibitor. Ethyl acetate fraction of blueberry exhibited antioxidant capacity.

Keywords

References

  1. Aniya, Y. and Naito, A. : Oxidative stress-induced activation of microsomal glutathion-S-transferase in isolated rat liver, Biochem. Pharm. 45: 37-43, 1993.
  2. Plastow, S. R., Lovell, C. R. and Young, A. R. : UVB-induced collagen changes in the skin of the hairless albinomouse, J. Invest. Dermatol. 88: 145-148, 1987.
  3. Branen, A. L. : Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene, J. Am. Oil. Chem. Soc. 52: 59-62, 1972.
  4. Halliwell, B. and Gutteridge, J. M. : Role of free radicals and catalytic metal ions in human disease: An overview. Methods Enzymol, 186: 1-85. 1990.
  5. Kasuga, A., Aoyagi, Y. and Sugahara, T. : Antioxidants activities of edible plants. Nippon Shokuhin Kogyo Gakkaish, 35: 852-856. 1988.
  6. Cha, Y. J. and Cho, Y. S. : Antioxidative activity of extracts from fruit of Cudrania tricuspidata. J. Kor. Soc. Food Sci. Nutr. 30: 547-551, 2001.
  7. Kang, M. H. et al. : Antioxidative activity of ethanol extract prepared from leaves, seed, branch and aerial part of Crotalaria tsessiflora L. Kor. J. Food Sci. Tech. 30: 547-551, 2002.
  8. Xing, Q., Kadota, S. Tadata, T. and Namba, T. : Antioxidative effects of phenylethanoids from Cistanche deserticola, Biol. Pharm, Bull. 19: 1580-1585, 1996.
  9. Chun, H. J., Kim, I. K. and Woo, W. H. : Inhibitory effects on retinoic acid and melanization of B16 melanoma cell by Epimedium koreanum Nakai and a-MSH. J. Kor. Chem. Soc. 44: 533-537, 2000.
  10. Chun, H. J., Hwang, S. G., Lee, J. S., Baek, S. H., Jeon, B. H. and Woo, W. H. : Inhibitory effect of butyl alcohol extract from Caesalpinia sappan L. on melanogenesis in melan-a cells Kor. J. Pharmacogn. 33: 130-136, 2002.
  11. Zheng, W. and Wang, S. Y. : Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries, and ligoberries, J. Agris. Food Chem. 51(2): 502-509, 2003.
  12. Joseph, J. A., Denisova, N. A., Bielinski, D., Fisher, D. R. and Shukitt-Hale, B. : Oxidative stress protection and vulnerability in aging: Putative nutritional implications for intervention. Mechanism of aging and Development 116: 141-153, 2000.
  13. Sellappan, S., Akoh, C. C. and Krewer, G. : Phenolic compounds and antioxidant capacity of Georgia-grown blueberries and blackberries, J. Agric. Food Chem. 50: 2432-2438, 2002.
  14. Howell, A. B. : Cranberry proanthocyanidines and the maintenance of urinary trat health, Crit. Rev. Food Sci. Nutr. 42: 273-278, 2002.
  15. Connor, A. M., Luby, J. J., Hancock, J. F., Berkeimer, S. and Hanson, E. J. : Changes in fruit antioxidant among blueberry cultivars during cold-temperature storage, J. Agric. Food Chem. 50(4): 893-898, 2002.
  16. Kalt, W., Ryan, D. A. J., Duy, J. C., Prior, R. L., Ehelenfeldt, M. K. and Kloet, S. P. V. : Interspecific variation in anthocyanins, phenolics, and antioxidant capacity among genotypes of highbush and lowbush blueberries, J. Agric. Food Chem. 49: 4761-4767, 2001.
  17. Wang, S. Y. and Jiao, H. : Scavenging capacity of berry crops on superoxide radicals, hydrogen peroxide, hydroxy radicals and singlet oxygen, J. Agric. Food Chem. 48(11): 5677-5684, 2000.
  18. Mcghie, T. K., Ainge, G. D., Barnett, L. E., Cooney, J. M. and Jensen, D. J. : Anthocyanin glycosides from berry fruit are absorbed and excreted unmetabolized by both humans and rats, J. Agric. Food Chem. 51: 4539-4548, 2003.
  19. Yoshida, T., Mori, K., Hatano, T., Okumura, T., Ushara, I., Komagoe, K.,Fujuita, Y. and Okuda, T. : Studies on inhibition mechanism of autooxidation by tannins and flavonoids. Chem. Pharm. Bull. 37(7): 1919-1923, 1989.
  20. Oyaizu, M. : Studies on products of browning reaction: Antioxidative activity of products of browning reaction, Japanese J. Nut. 44(6): 307-312, 1986.
  21. Yun, K. A., Park, Y. J. and Bae, S. J. : Antioxidant and tyrosinase inhibitory effects of Brassica oleracea L. fractions, J. Korean Soc. Food Sci. Nutr. 33(1): 7-15, 2004.
  22. Hearing, V. J. : Biochemical control of melanogenesis and melanosomal organization, Soc. Invest. Dermatol. 4(1): 24-27, 1999.