• 한국작물학회지
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• 제59권3호
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• pp.263-281
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• 2014

전사인자는 식물에서 유전자 발현을 조절하기 위해 필수적이며, 유전자의 promoter나 enhancer 부위에 결합하며, 기본 전사 조절, 전사의 향상, 발달, 세포내 신호전달, 환경에 반응, 세포 주기의 조절 등의 역할을 수행한다. 옥수수 게놈의 염기서열 분석은 전사인자의 유전자 발현 조절의 기작을 이해하는데 도움을 줄 것으로 기대된다. 과거 옥수수의 전체 게놈의 중복으로 옥수수에서 4,000개 이상의 전사인자가 코딩 될 것으로 예상된다. 본 논문에서는 옥수수의 ABI3/VP1, AP2/EREBP, ARF, ARID, AS2, AUX/IAA, BES1, bHLH, bZIP, C2C2-CO-like, C2C2-Dof, C2C2-GATA, C2C2-YABBY, C2H2, E2F/DP, FHA, GARP-ARR-B, GeBP, GRAS, HMG, HSF, MADS, MYB, MYB-related, NAC, PHD, WRKY 전사인자의 특징을 간략히 서술하고, 전사인자의 염기서열을 분석하여 sequence logo를 통하여 각각의 도메인을 표시하였다. 이러한 전사인자 및 관련된 유전자의 분자생물학적 연구는 옥수수에서 중요한 기능을 하는 유전자의 발굴 및 육종을 위한 목표 유전자의 선발에 도움을 줄 것으로 기대된다.

• 한국해양공학회지
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• 제24권6호
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• pp.103-108
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• 2010

WIG crafts are high speed vessels with the features of a dynamic supported craft. These crafts, which are predominantly lightweight and operate at substantially greater speeds than conventional craft, could not be accommodated under traditional maritime safety instruments. WIG crafts inherently possess more hazard factors than conventional ships because of their relatively high speed, lightweight, and navigational characteristics, and an accident is likely to cause damage to the ship and a high loss of life. Because WIG crafts are composed of many systems and subsystems, the safety assessment of a WIG must use a commercial software system in the design stage. This paper reviews a safety assessment process and methodology proposed by the IMO interim guideline, which were developed in view of the configuration of WIG crafts. This safety assessment system was developed to fit the WIG's safety assessment process using a reliability analysis system widely used in commercial systems. The FHA was performed on the functional hazards of systems in the conceptual design stage.

• Molecules and Cells
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• 제27권3호
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• pp.359-363
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• 2009

Chfr, a checkpoint with FHA and RING finger domains, plays an important role in cell cycle progression and tumor suppression. Chfr possesses the E3 ubiquitin ligase activity and stimulates the formation of polyubiquitin chains by Ub-conjugating enzymes, and induces the proteasome-dependent degradation of a number of cellular proteins, including Plk1 and Aurora A. While Chfr is a nuclear protein that functions within the cell nucleus, how Chfr is localized in the nucleus has not been clearly demonstrated. Here, we show that nuclear localization of Chfr is mediated by nuclear localization signal (NLS) sequences. To reveal the signal sequences responsible for nuclear localization, a short lysine-rich stretch (KKK) at amino acid residues 257-259 was replaced with alanine, which completely abolished nuclear localization. Moreover, we show that nuclear localization of Chfr is essential for its checkpoint function but not for its stability. Thus, our results suggest that NLS-mediated nuclear localization of Chfr leads to its accumulation within the nucleus, which may be important in the regulation of Chfr activation and Chfr-mediated cellular processes, including cell cycle progression and tumor suppression.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2001년도 추계학술대회 및 정기총회
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• pp.91-91
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• 2001

Nramp2, also known as DMT1 and DCT1, is a 12-transmembrane domain protein responsible for dietary iron uptake as well as metal ions such as lead, manganese, zinc, copper, nickel, cadmium, and cobalt. High expression of DMT1 increase lead uptake, and DMT1-dependent lead transport was H -dependent and inhibited by iron ions. The molecular mechanism of lead transport in CNS is as yet unknown. although interactions between iron and lead at the level of absorption have been known for some time. The process of lead uptake into astrocytes was not known yet. Nramp2 may mediate transport of heavy metal into astrocytes. We investigated whether Nramp2 mediate transport of lead into astrocytes. And we do whether Nramp2 was expressed highly by deprivation of iron in Astrocytes, and lead uptake into astrocytes was influenced by expression of Nramp2. Immortalized human fetal astrocyte(SV-FHA) cells were cultured in medium containing Dulbecco's modified Eagle's medium and treated with Deferoxamine. Northern blot analysis was done for determining mRNA level of DMT1 and lead uptake assay was done in incubation condition of pH 5.5 and 7.4.

• 한국화재소방학회:학술대회논문집
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• 한국화재소방학회 2012년도 춘계학술발표회 초록집
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• pp.109-112
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• 2012

원자력발전소는 타 산업시설에 비해 화재발생 가능성이 낮으나 방사성물질 누출가능성을 최소화하기 위하여 심층방어와 다중방호 설계를 통한 안전성확보가 매우 중요하다. 이를 위해 국내에서는 화재위험도분석(FHA)과 안전정지능력분석(SSA) 및 화재 확률론적안전성분석(Fire PSA)을 수행하고 있으며, 이 중 화재 PSA는 주요 화재구역 선별, 구역별 화재발생빈도 및 기기손상확률 계산, 화재사고 경위분석 및 화재취약성 파악 등을 분석한다. 본 논문에서는 미국 원자력규제위원회(USNRC)와 전력연구소(EPRI)가 공동 연구개발한 화재 PSA 방법론인 NUREG/CR-6850 기법을 적용하여, 화재 PSA에 필요한 화재점화빈도(Fire Ignition Frequency)를 정량적으로 계산하였다. 정확한 결과값을 도출하기 위해 매크로를 이용한 프로그램인 FIFA(Fire Ignition Frequency Analyzer)를 개발하였으며, 향후 국내 원전 화재 PSA 분석업무에 유용하게 사용될 수 있을 것으로 기대된다.

• 전기학회논문지
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• 제65권12호
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• pp.1992-1999
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• 2016

The plasma process is applied to various industrial fields such as high-tech IT industry, textiles and medical. Therefore, there is increasing interest in the plasma power supply, and demand for power devices of high efficiency and high power density is increased. Plasma power supply for process must solve the arc problem, when the plasma is unstable. The output capacitor is closely related to the arc problem. If the output capacitor is smaller, the damage from the arc problem is reduced. However, the small value of the output capacitor affects the operating characteristics of the power supply. In this paper, a LLC resonant converter is adopted, because it can achieve high efficiency and power density in the plasma DC power supply. However, due to the small value of the output capacitor, the converter is operated as a LLCC resonant converter. Therefore, a gain characteristic of LLCC resonant converter is analyzed by using the FHA (First Harmonic Approximation) in plasma power supply. Simulation and experimental results are presented to verify the characteristic analysis of LLCC Resonant Converter.

• Nuclear Engineering and Technology
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• 제51권7호
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• pp.1765-1775
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• 2019

Multiple spurious operations (MSOs) mean multiple fire induced circuit faults causing an undesired operation of one or more systems or components. The Nuclear Energy Institute (NEI) of the United States published NEI 00-01 as guidelines for solving MSOs. And this guideline includes MSO scenarios of pressurized water reactor (PWR) and boiling water reactor (BWR). Nuclear power plant operators in U.S. analyzed MSOs under MSO scenarios included in NEI 00-01 and operators of PWRs in Korea also analyzed MSOs under the scenarios of NEI 00-01. As there are no pressurized heavy water reactors (PHWRs) in the United States, MSO scenarios of PHWRs are not included in the NEI 00-01 and any feasible scenarios have not been developed. This paper developed MSO scenarios which can be applied to PHWRs by reviewing the 63 MSO scenarios included in NEI 00-01. This study found that seven scenarios out of the 63 MSO scenarios can be applied and three more scenarios need to be developed.

• 한국화재소방학회논문지
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• 제18권4호
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• pp.64-71
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• 2004

본 논문에서는 NIST에서 개발한 CFAST 화재 모델링 코드를 사용하여 원자력발전소 펌프실 화재를 모의하였다. 급기 제한, 최소 산소 농도(Lower Oxygen Limit), 복사열 방출비율(Radiative Fraction), 방화문 개방 정도 등의 CFAST 입력 변수 변화에 따라 화재 성장 변화를 분석하였다. 분석 결과에 의하면 본 화재구역에서의 화재는 환기 지배형 화재이므로 급기 제한 및 최소 산소 농도 10%로 설정하는 것이 타당한 것으로 판단되며, 복사열 방출에 따라 상부층 가스 온도는 큰 변화가 없는 것을 알 수 있었다. 화재가 발생한 화재구역 내 펌프를 제외하고는 상부에 위치한 케이블은 모두 건전성을 유지하는 것으로 나타났으며, CCDP(Conditional CDP)를 평가한 결과 9.25E-07로, 화재위험도분석보다 현실적이고 불확실성이 감소한 결과를 도출할 수 있었다.

• 시스템엔지니어링학술지
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• 제6권1호
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• pp.1-13
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• 2010

KC-100 is a 4 seats, single piston engine, civil aircraft whose type certificate is applied for KAS 23 (FAR 23) for the first time in Korea. Its system safety assessment and analysis have been conducted to meet the minimum safety requirement in KAS 23 and to verify the safety of equipment, system, and installation in accordance with the requirement of ${\S}$23.1309 and the guidelines in FAA AC 23.1309-1D and SAE ARP 4761. This safety assessment begins with the FHA (Functional Hazard Assessment) at aircraft and system level in preliminary design phase, and all of the safety assessment and analysis reports including the preliminary version of SSA (System Safety Assessment) have been prepared during detail design phase. The revised version of these safety reports will be approved by Airworthiness Authority through the ground and flight test phases. In this paper, the safety assessment requirement in ${\S}$23.1309, safety assessment guideline in AC 23.1309-1D, and safety assessment and analysis methods in ARP 4761 will be explained based on the application example for KC-100 development. The experience and knowledge of this system safety assessment for civil aircraft can be applied to commuter aircraft of FAR 23 class or large transport airplane of FAR 25 class.

• 지질공학
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• 제22권3호
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• pp.353-363
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• 2012

울릉도는 강도가 약한 화산암과 강도는 높으나 수직절리가 잘 발달하는 조면암질암으로 구성되어 있으며, 이러한 지질학적 특성으로 인해 낙석이 빈번하게 발생하고 있어 일주도로의 이용에 큰 위험요소로 작용하고 있다. 본 연구에서는 낙석 재해위험도가 높은 울릉도 북동부의 약 3 km 구간을 대상으로 3종류의 낙석재해 위험도 평가법을 이용하여 낙석재해 위험도 평가를 수행하고 재해도를 작성하였다. 사용된 평가표는 일본 도로방재 총점검에서 적용된 낙석위험도 조사표, 일본 고속도로조사회의 낙석위험도 평가표, 그리고 미국연방도로국의 RHRS (Rockfall Hazard Rating System)이다. 도로사면의 지형 지질학적 조건을 고려하여 27개 구간을 설정하여 평가한 결과, 20개 사면(약 74%)에서 낙석재해 위험도가 높음과 보통으로 평가되었다. 이러한 조사결과를 바탕으로 낙석재해 위험도를 작성하였다.