• 한국산림과학회지
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• 제107권1호
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• pp.16-24
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• 2018

본 연구는 갯버들의 생장 특성을 기반으로 단기순환벌채림(Short rotation coppices)을 조성하고, 바이오매스 생산 및 자원화 방안을 모색하기 위하여 우수집단 및 개체를 선발하고자 수행하였다. 갯버들이 서식하는 7개 집단에서 시료를 수집하여 2개의 시험지(춘천과 용인)에 집단별로 시험구를 설치하고, 우수집단 및 개체선발에 있어서 각 요인이 미치는 유의적인 차이를 조사하였다. 수고와 근원경은 시험지, 수집집단 및 연간생장 간에 통계적으로 유의한 차이를 보였다(p<0.01). 식재 3년 후 지상부의 수확량은 춘천시험지에서는 영월집단이 개체 당 평균 4.8 kg(dw)으로 나타났으며, 이는 각 집단별 전체 평균의 2배 이상의 수확량으로 보여진다. 용인시험지에서는 홍천과 원주집단의 수확량이 개체 당 평균 3.3kg(dw)으로 가장 높게 나타났다. 두 시험지에서 수확량이 높게 나타난 집단은 일치하지 않았으나 수집 집단(Pop)과 시험지(Nur) 간의 상호작용에서 유의성이 인정되어서, 각 시험지별로 수확량이 우수한 집단을 제시할 수 있었다(F value = 3.51, p<0.01). 이러한 결과를 토대로 선발된 집단의 영양계 검정을 이용하여 목질계 바이오매스 자원화를 위한 우수 자원을 육성할 수 있을 것이다.

• Clinical and Experimental Reproductive Medicine
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• 제27권4호
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• pp.345-351
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• 2000

Objective: Hatching of the blastocyst from the zona pellucida (ZP) is a key event in mammalian implantation. In vivo, two factors have been identified as possible mediators of hatching: lysis of the ZP by substances elaborated either from the embryo or female reproductive tract and pressure exerted on the zona by expansion of the blastocyst. Two methods of zona manipulation were already in use to enhance the ability of embryos to hatch: mechanical PZD and chemical ZD by acidic Tyrode's solution. But several controversies of each method have been reported. The purpose of this study was to investigate the effect of pronase for mouse embryo hatching. Methods: Mouse embryos were obtained following ovulation induction of $F_1$ animals. Fresh and cryo-thawed morula embryos were exposed to 0.5, 1.0, 2.0, 5.0 ${\mu}g/ml$ pronase in Ham's F10 for 72 hrs. Main outcome measures were the rates of partial hatching and completely hatched blastocysts, and cell number of it. Results: In fresh and cryo-thawed group, the rates of completely hatched blastocyst were significantly higher in 5 ${\mu}g/ml$ pronase treatment group than control group. There was no difference in completely hatched blastocyst total cell number between pronase treatment group and control group. This suggest that pronase treatment did not harmful in mouse embryo development. In pronase treatment group, zona pellucida were thinner than control group. Conclusion: The addition of pronase to culture media may accelerate the hatching of embryo. So, enzymatic treatment of the zona may provide a valuable and effective assisted hatching technique for human in-vitro fertilization-embryo transfer.

• Molecules and Cells
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• 제25권3호
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• pp.417-427
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• 2008

Comparison of maps and QTLs between populations may provide us with a better understanding of molecular maps and the inheritance of traits. We developed and used two reciprocal $BC_1F_1$ populations, IP/DS//IP and IP/DS//DS, for QTL analysis. DS (Dasanbyeo) is a Korean tongil-type cultivar (derived from an indica x japonica cross and similar to indica in its genetic make-up) and IP (Ilpumbyeo) is a Korean japonica cultivar. We constructed two molecular linkage maps corresponding to each backcross population using 196 markers for each map. The length of each chromosome was longer in the IP/DS//IP population than in the IP/DS//DS population, indicating that more recombinants were produced in the IP/DS//IP population. Distorted segregation was observed for 44 and 19 marker loci for the IP/DS//IP and IP/DS//DS populations, respectively; these were mostly skewed in favor of the indica alleles. A total of 36 main effect QTLs (M-QTLs) and 15 digenic epistatic interactions (E-QTLs) were detected for the seven traits investigated. The phenotypic variation explained (PVE) by M-QTLs ranged from 3.4% to 88.2%. Total PVE of the M-QTLs for each trait was significantly higher than that of the E-QTLs. The total number of M-QTLs identified in the IP/DS//IP population was higher than in the IP/DS//DS population. However, the total PVE by the M-QTLs and E-QTLs together for each trait was similar in the two populations, suggesting that the two $BC_1F_1$ populations are equally useful for QTL analysis. Maps and QTLs in the two populations were compared. Eleven new QTLs were identified for SN, SF, GL, and GW in this study, and they will be valuable in marker-assisted selection, particularly for improving grain traits in tongil-type varieties.

• KSBB Journal
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• 제18권6호
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• pp.529-535
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• 2003

본 연구에서는 등$.$경유 혼합물을 Pseudomonas aeruginosa F722를 이용하여 분해시킬 때 생분해율에 미치는 생물계면활성제, 화학계면활성제 및 공기 공급량의 영향을 조사하였다. 그 결과, 탄화수소 분해율은 0.01%와 0.15% 농도의 생물계면활제를 첨가하였을 때가 0.05%, 0.1% 및 0.2%농도의 생물계면활제를 첨가하였을 때보다 최고 6.2% 높은 94.3, 94.2% 제거율을 나타냈다. 하지만, 0.15% 생물계면활제를 첨가하였을 때가 0.01% 생물계면활제를 첨가하였을 때보다 탄화수소 분해율이 더 안정적이었다. 그리고 생물계면활성제 (surface tension; 30mN/m)와 화학계면활성제 (Tween 80;39mN/m, detergent;31mN/m)를 0.15% 농도로 첨가하여 배양하였을때, 탄화수소 분해율은 94.2, 93.5, 93.4%로 비슷하였다. 하지만, P. aeruginosa F722의 개체수는 생물계면활성제를 첨가했을 때가 화학계면활성제를 첨가했을 때보다 2배 이상 증가된 19 ${\times}$ $10^{7}$ cfu/$m\ell$로 조사되었다. 0.5vvm으로 공기를 공급하면서 교반을 수행하였을 때, 배양 3일 후, 사용균주를 접종하지 않은 공시험에서 탄화수소 분해율은 68.8%였으며, P. aeruginosa F722를 접종하였을 때는 94.8%이었다. 0.5 vvm으로 공기를 공급하였을 때가 공기를 공급하지 않았을 때보다 배양시간이 1/3로 단축되었다. 그리고 교반배양 (3일)과 정치배양 (10일)에서 탄화수소 분해율은 각각 94.8, 93.7%였다.

• 농업과학연구
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• 제41권1호
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• pp.41-46
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• 2014

본 연구는 국내 주요 두록 종돈장의 혈통자료를 이용하여 근교수준과 유효집단 크기를 추정하기 위해 수행하였다. 혈연관계를 가지기 시작한 1987년부터 2013년까지 총 86,639두의 혈통자료를 최종적으로 이용하여 6개 종돈장의 세대별 근교계수 및 유효집단크기를 추정하였다. 종돈장별 세대에 따른 근교계수 변화는 2가지 패턴으로 조사되었으며, 근교계수 평균은 5% 이내로 적절한 수준을 유지하는 것으로 사료된다. 유효집단 크기는 종돈장별 다양한 차이를 보였지만 모든 종돈장이 최소권장수준인 50두 이상으로 유지하는 것으로 분석되었다. 대부분의 종돈장이 낮은 근교수준을 유지하는 것으로 조사되었는데, 근교계수를 고려한 교배관리와 외부 종돈 도입이 주된 원인이라 사료된다. 외부 종돈을 수입해서 도입하는 경우, 집단의 유전적 다양성은 증가할 수 있지만, 향후 국제적 종자주권 행사에는 불리한 상황이 될 수 있다. 특히 나고야의정서의 ABS(Access, Benefit, Sharing) 규정(2010)에 따라 유전자원을 제공하는 업체나 국가가 종자 주권 행사에 유리하도록 전개되고 있다. 그리고 업체상호 간 계약합의가 국제거래 규약의 효력을 가지기 때문에 정액, 자손 등 종자의 사후활용에 대해서도 로열티를 요구할 수 있다. 따라서 유효집단 크기를 높이기 위한 방법으로 무분별한 수입보다는 국내 종돈장 사이에서 우수 종돈을 공유하는 것이 필요할 것으로 사료된다.

• 생명과학회지
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• 제30권9호
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• pp.798-803
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• 2020

1960년대 후반 인디카 품종과 자포니카 품종의 교잡에 의하여 새로운 통일형 벼 품종을 육성했고 그 재배기술을 확립했으며, 현재까지 이 재배기술을 이용하여 소비자들의 기호에 맞게 그리고 극한환경에 대응할 수 있는 다양한 품종들이 만들어졌다. 서로 다른 작물끼리 교배하여 얻은 F₁을 가지고 반복적인 세대 진전을 통하여 도입을 원하는 우량한 유전자원의 형질이 더 이상 분리되지 않을 때 교배시킨 형질이 고정되었다고 볼 수 있으며 비로소 신품종이 될 수 있다. 본 연구에서는 가뭄에 저항성을 띠는 HV23 계통을 모품종인 Ilmi와 교배한 집단과 벼멸구에 내성을 갖는 Drimi2ho와 교배한 집단의 F₄, F₅, 그리고 F₆의 농업형질을 조사하여 고정 여부를 확인했다. HV23와 Drimi2ho/HV23 계통의 F₅, F₆를 비교하였을 때 그리고 Ilmi/HV23 계통의 F₄, F₅를 비교하였을 때 모두 유의하지 않은 정도의 형질 변이가 나타났기 때문에 농업형질이 고정되었다고 판단할 수 있었다. 일반적인 계통 육종법은 품종개량에 10년 이상의 시간이 드는 반면 본 연구에서는 4-5년 만에 형질이 고정되었음을 확인하였다.

• 가정∙방문간호학회지
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• 제4권
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• pp.76-85
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• 1997

In modern society, the human average life span has been prolonged due to medical benefits and changes in society, which results in the rapid and world -wide increase in the population of elderly. Consequently, the field of nursing science, as well as the field of many other discipline, has shown increasing interests in issues on the elderly. In addition, to improve the quality of life for elderiy people a great deal of effort has been made. The purpose of our study is to analyze the correlation between family support and quality of life in order to develop basic data for nursing interventions to maintain life satisfaction of the elderly. The sample consists of 108 subjects residing at home whose ages are over 65 years old. The data has been collected, from November 11, 1996 to November 23, 1996, through interviewing the elderly using a questionnarie. For the family supoort we used two: 1) the 5 - point Likert scale questionnarie developed by Gallo and Warren (Family support I) and 2) the 5-point Likert scale developed by H. S. Kang(Family support II), and for the quality of life we used the 3 - point likert scale questionnarie developed by Choi, Young Hee. For data analysis we used percentages, means, Pearson Correlation Coefficient and ANOVA. The results of our study are as follows: 1. For perceived family support I & II, the minimum score is 12 & 19, the maximum score is 32 & 46, the mean score is 24.49 & 34.90, respectively. 2. For Quality of life, the minimum score is 13, the maximum score is 39, the mean score is 28.61. 3. there is a very strong correlation between the perceived family support of the subjects and Quality of life (for I r=0.35047, p<.001 and for II r= 0.60558, p<.001). 4. The relationship between the general characteristics of the elderly and our two variables. family support and Quality of life, is as follows: 1) According to age(for II F=5.32, p<.01), the amount of monthly pocket money(for II F= 3.52, p<.05), inmate(for I F=2.93, p<.05, for II F=2.84, p<.05), economics(for I F=8.99, p<.01. for II F=7.51. p<.01), supporter(for I F=4.01. p<.01. for II F=3.43, p<.01), there is a statistically significant difference in family support. 2) According to the amount of monthly pocket money(F=6.69, p<.01), inmate(F=2.24, p<.05), economics(F=15.38, p<.01), there is a statistically significant difference in Quality of life. In conclusion, it can be said that the family support is an important variable to the Quality of the elderly life.

• 근관절건강학회지
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• 제7권1호
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• pp.5-24
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• 2000

Middle age for women is one of the most important stages of the whole normal life span and has unique problems concerning the psychological and physical health, specifically degenerative musculoskeletal diseases. Therefore middle age women should prepare in order to lead a healthy and fruitful life as they enter/begin old age. As the population of middle age women increase, the demands of nurses who care for this age group also continue to glow. Nurses must be interested in this middle age group of women in Korea. This study was designed to identify the extent of family support and self-esteem of the middle age women with degenerative musculoskeletal diseases and the relationship between them and nurse's contribution to middle age women's health promotion that maintain and promote qualify of her life. The population of this study was 112 women aged 40-60, attending the out patient clinic one University Hospital and one Hospital, in Pusan. The data was collected from 1st August to the 30th of September, 1998 by using 36 items questionnaire. The instrument used for measuring family support was of Chai's(1983) FSS which was modified by Kang's(1984) Family Support Scale. And that for self-esteem was Rosenberg's(1965) Self-esteem Scale. The reliability of Chai's FSS and Rosenberg's instruments were tested by Cronbach's alpha and showed that they were 0.93 and 0.89 each respectively. Data was analyzed by descriptive statistics, t-test, ANOVA, and Pearson's Correlation Coefficient using the SPSS computer program. The results of the study were summarized as follows : 1. The mean score of the perceived family support was $39.31{\pm}9.42$. 2. The mean score of the perceived self-esteem was $33.16{\pm}6.75$. 3. Statistically significant factors influencing the family support among sociodemographic variables was satisfaction of marriage(F=-3.069, p<0.003). 4. Statistically significant factors influencing the self-esteem among sociodemographic variables were age(F=3.992, p=0.000), education(F=2.260, P=0.026), housing (F=-1.987, P=0.049) and satisfaction of marriage(F=-2.305, P=0.023). 5. There was a significant correlation between family support and self-esteem (r=.621, p<.000). In conclusion, perceived the family support was important for middle age women to increase their self-esteem. Nurses should be aware of the necessity of family support in the supportive nursing of middle age women and should be able to make a plan to educate the family about supporting the wife/mother. The continuing study of the health promotion of middle age women to find out variables influencing middle age women' health must continue.

• 원예과학기술지
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• 제35권2호
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• pp.232-242
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• 2017

The goal of marker-assisted backcrossing (MAB) is to significantly reduce the number of breeding generations required by using genome-based molecular markers to select for a particular trait; however, MAB systems have only been developed for a few vegetable crops to date. Among the types of molecular markers, SNPs (single-nucleotide polymorphisms) are primarily used in the analysis of genetic diversity due to their abundance throughout most genomes. To develop a MAB system in Chinese cabbage, a high-throughput (HT) marker system was used, based on a previously developed set of 468 SNP probes (BraMAB1, Brassica Marker Assisted Backcrossing SNP 1). We selected a broad-spectrum TuMV (Turnip mosaic virus) resistance (trs) Chinese cabbage line (SB22) as a donor plant, constructing a $BC_1F_1$ population by crossing it with the TuMV-susceptible 12mo-682-1 elite line. Foreground selection was performed using the previously developed trsSCAR marker. Background selection was performed using 119 SNP markers that showed clear polymorphism between donor and recipient plants. The background genome recovery rate (% recurrent parent genome recovery; RPG) was good, with three of 75 $BC_1F_1$ plants showing a high RPG rate of over 80%. The background genotyping result and the phenotypic similarity between the recurrent parent and $BC_1F_1$ showed a correlation. The plant with the highest RPG recovery rate was backcrossed to construct the $BC_2F_1$ population. Foreground selection and background selection were performed using 169 $BC_2F_1$ plants. This study shows that, using MAB, we can recover over 90% of the background genome in only two generations, highlighting the MAB system using HT markers as a highly efficient Brassica rapa backcross breeding system. This is the first report of the application of a SNP marker set to the background selection of Chinese cabbage using HT SNP genotyping technology.

• Asian Pacific Journal of Cancer Prevention
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• 제14권10호
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• pp.6181-6184
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• 2013

Background: Lung cancer is the most frequent cancer in males and the fourth most frequent site in females, worldwide. This study is the first to explore the profile of lung cancer in Kuwait. Materials and Methods: Cases of primary lung cancer (Kuwaiti) in Kuwait cancer Registry (KCR) were grouped in 4 periods (10 years each) from 1970-2009. Epidemiological measures; age standardized incidence rate (ASIR) with 95% confidence intervals (CI), Standardized rate ratio (SRR) and Cumulative risk and Forecasting to year 2020-2029 used for analysis. Results: Between years, 2000-2009 lung cancer ranked the 4th and the 9th most frequent cancer in males and females respectively. M:F ratio 1:3. Mean age at diagnosis (95%CI) was 65.2 (63.9-66.4) years. The estimated risk of developing lung cancer before the age of 75 years in males is 1.8% (1/56), and 0.6 (1/167) in females. The ASIR for male cases was 11.7, 17.1, 17.0, 14.0 cases/100,000 population in the seventies, eighties, nineties and in 2000-2009 respectively. Female ASIR was 2.3, 8.4, 5.1, 4.4 cases/100,000 population in the same duration. Lung cancer is the leading cause cancer death in males 168 (14.2%) and the fifth cause of death due to cancer in females accounting for 6.1% of all cancer deaths. The ASMR (95%CI) was 8.1 (6.6-10.0) deaths/100,000 population and 2.8 (1.3-4.3) deaths/100,000 population in males and females respectively. The estimated Mortality to incidence Ratio was 0.6. Conclusions: The incidence of lung cancer between years 2000-2009 is not different from that reported in the seventies. KCR is expecting the number of lung cancer cases to increase.