• Title/Summary/Keyword: F1 generation

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A Study on Toxicity Bio-markers of a Mouse using Combustion Gas SO2 generated from Fire (마우스(mouse)를 이용한 건축물 마감재료 연소가스 SO2의 독성생체지표 연구)

  • Rie, Dong-Ho;Cho, Nam-Wook;Choi, Soon-Young
    • Journal of the Korea Safety Management & Science
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    • v.14 no.1
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    • pp.43-51
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    • 2012
  • This study was carried out to observe the impacts of a mouse's inhalation of toxic gas SO2 generated from combustion on its organs by different concentrations. As for research methods: First, after concentrations of SO2 generation from combustion had been set to three: low (10.4 ppm), middle (24.9 ppm) and high (122 ppm) through Gas Toxicity Testing Method (KS F 2271) and SO2 combustion gas was exposed to eight mice in each concentration. Five mice that were able to move based on LD50, a criterion, which sets the down time of a mouse's average behaviors to over 9 minutes, were randomly selected in each concentration, and they were set up as the subjects of the study on toxicity bio-markers. Second, tissues were taken from heart, liver, lungs, spleen and the thymus gland of the mice selected in each concentration and a pathological examination of them was carried out. As a result, microvascular congestion appeared in the heart, and cell necrosis, cortex congestion and tubule medulla congestion, etc. in each concentration were observed in addition to vascular congestion in liver, lungs, spleen and the thymus gland. Also, it was found that the higher the concentrations of SO2 exposure is, the greater, the changes in the organs get. Through this study, SO2 of various toxic gases generated from fire turned out to affect the tissues of each organ of a mouse, it is expected that the toxic gases may greatly affect human body in case of actual fire, and this study is evaluated as having a significance as a basic data on inhalation toxicity assessment of toxic substances generated in combustion.

Preparation of Si/C Anode with PVA Nanocomposite for Lithium-ion Battery Using Electrospinning Method

  • Choi, Sung Il;Lee, Ye Min;Jeong, Hui Cheol;Jung, Eun-Jin;Lee, Mi Sun;Kim, Jinyoung;Kim, Yong Ha;Won, Yong Sun
    • Korean Chemical Engineering Research
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    • v.56 no.1
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    • pp.139-142
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    • 2018
  • Silicon (Si) is a promising anode material for next-generation lithium ion batteries (LIBs) because of its high capacity of 4,200 mAh/g ($Li_{4.4}Si$ phase). However, the large volume expansion of Si during lithiation leads to electrical failure of electrode and rapid capacity decrease. Generally, a binder is homogeneously mixed with active materials to maintain electrical contact, so that Si needs a particular binding system due to its large volume expansion. Polyvinyl alcohol (PVA) is known to form a hydrogen bond with partially hydrolyzed silicon oxide layer on Si nanoparticles. However, the decrease of its cohesiveness followed by the repeated volume change of Si still remains unsolved. To overcome this problem, we have introduced the electrospinning method to weave active materials in a stable nanofibrous PVA structure, where stresses from the large volume change of Si can be contained. We have confirmed that the capacity retention of Si-based LIBs using electrospun PVA matrix is higher compared to the conservative method (only dissolving in the slurry); the $25^{th}$ cycle capacity retention ratio based on the $2^{nd}$ cycle was 37% for the electrode with electrospun PVA matrix, compared to 27% and 8% for the electrodes with PVdF and PVA binders.

Isolation of Flavobacterium multivorum Producing Exo-dextranase (세포외 덱스트란 분해효소를 생산하는 Flavobacterium multivorum의 분리)

  • 정재호;이형환;김영희;이희무
    • Korean Journal of Microbiology
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    • v.25 no.4
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    • pp.346-352
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    • 1987
  • One hundred and seventeen colonies were screened for the detection of the production of exodextranase on the dextran-mineral salts medium. Ten colonies out of them produced the dextranase. Flavobacterium multivorum greatly producing the enzyme was isolated from soil, identified and then studied for various biochemical characteristics. The activity of the dextranase in the cultured medium was high between pH8 and 9 at $35^{\circ}C$, and between $45^{\circ}C$ and $55^{\circ}C$ at pH8. By the growth curves the generation times of the bacterium were approximately 52 minutes in the LB broth, 38 minutes in the LB plus 1% dextran and 660 minutes in the dextran-salts. The strain did not have ant plasmid, and was susceptible to genramicin, cotrimoxazole and cefoperazone, and moderately susceptible to chloramphenicol, cefamandole and cefotaxime, but resistant to ampicillin, cephalothin, tetracycline, amikacin and tobramycin.

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Fertility and General Reproductive Ability Test of Ginkgo Biloba Extract (EGb 761) in Rats

  • Lee,Yong-Soon;Nam, Jeong-Seok;Yang, Jae-Man;Che, Jeong-Hwan;Kang, Byeong-Chul;Lee, Hak-Mo;Park, Jae-Hak;Kim, Dai-Yong;Kang, Sung-An
    • Toxicological Research
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    • v.12 no.1
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    • pp.129-136
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    • 1996
  • A fertility and general reproductive a. bility study was performed in Sparque-Dawley rats intravenously injected with Ginkgo biloba extract (EGb 761), a potential pharmaceutical excipient, at dose levels of 7.5, 15, and 30 mg/kg/day. Male rats were treated with Ginkgo biloba extract (EGb 761)from 14 days before mating until 21 days after delivery. Female rats received extract for 2 months prior to mating. No abnormal signs were noted in mating or fertility of the rats treated with Ginkgo biloba extract (EGb 761). No significant external, visceral, and skeletal anomalies or mental and physical development attributable to treatment was noted in any fetuses examined. The fertility of F1 generation was not affected by the treatment also. It was concluded that Ginkgo biloba extract (EGb 761) has no harmful effect on mating, fertilization, implantation, embryonic development and normal physical development.

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Comparisons of Growth and Yield Characters between Near-isogenic Lines with Dark and Pale Green Leaves in Rice (수도 농녹색엽과 담녹색엽 Near-isogenic 계통의 생장특성 및 수량형질 비교)

  • Park, Sun-Zik;Im, Byeung-Gi;Lee, Byun-Woo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.31 no.2
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    • pp.226-230
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    • 1986
  • Two near-isogenic lines with dark and pale green leaves, derived from the F$\_$7/ generation of wxl26 cross were compared on photosynthetic capacity, growth parameters, grain yield and yield-related characters. Dark green-leaved lines contained much greater content of chlorophyll a and b than pale green-leaved ones, but chlorophyll a to b ratio showed no difference between them. The photosynthetic nte per unit leaf area was higher in dark green-leaved lines than in pale green-leaved ones in the flag leaves at heading stage, but that per unit chlorophyll content showed reversed result. The crop growth rate from transplanting to heading was consistantly higher in the dark green-leaved lines, resulting from their greater net assimilation rate. Dark green-leaved lines produced greater number of panicles and spikelets per hill, out yielding pale green-leaved lines, but ripened grain ratio and 1000-grain weight showed no differences between those lines.

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Generation of Transgenic Mice Overexpression Mouse RESISTIN

  • J. R. Chun;S. J. Song;J. T. Do;K. S. Chung;Lee, H. T.
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.99-99
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    • 2002
  • The hormone resistin is associated with typeII diabetes mellitus in rodent model. Resistin impairs glucose tolerance and insulin action. A new class of anti-diabetic drugs were called thiazolidinediones (TZDs) downregulates a resistin which is induced during adipocyte differentiation. But the connection between increased adiposity and resistin remains unknown. The objectives of this study was to clone a mouse resistin cDNA and to generate transgenic mice overexpressing mouse resistin gene. The 555 bp of mouse resistin was amplified from mob cDNAS by polymerase chain reaction (PCR) and cloned into pCR$\^$(R)/ 2.1 TOPO T-vector. Mouse resistin mRNA on the basis of Genbank sequence (acession no. AF323080). Then, the PCR product was cloned into pTargeT$\^$TM/ mammalian expression vector that has pCMV promoter and chimeric intron. Restriction enzyme analysis with BamH I and Not I was carried out to determine an orientation of the insert in the vector. The pCMV-mus/resistin gene was prepared from previous recombinant pTargeT$\^$TM/-mus/resistin by digestion of Bgl II, and has used for microinjection into pronuclei of one cell embryos. The microinjected embryos were transfered to pseudopregnant foster-mother. Mouse resistin expression was detected in transgenic F1 mice by Reverse Transcriptase- Polymerase Chain Reaction (RT-PCR). Resistin gene expression mouse has heavier body weight which was measured higher level of plasma glucose than that of normal mouse. And in diet-induced experiments, the abdominal fat pads were isolated from each 24h starvation and re-feeding after fasting group mice that were assessed by RT-PCR analysis. In fasting group mice, resistin expression was higher than that of re-feeding group mice. This result suggests that the resistin gene overexpressing mice may be became to obesity and be useful as an animal disease model to be diabetes mellitus caused by insulin resistance of resistin.

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Development of Deconstructive Upcycling Fashion Design Using the Design Method of Christopher Nemeth (크리스토퍼 네메스의 디자인 방법을 활용한 해체적 업사이클링 패션디자인 개발)

  • Hyojoon Moon;Jeonghyun Baek;Soojeong Bae
    • Journal of Fashion Business
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    • v.28 no.3
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    • pp.1-15
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    • 2024
  • Christopher Nemeth is a designer who combines characteristics of a pioneering upcycle design and deconstructionist design with fabrics, waste clothing, and pieces of fabric recycled in the 1980s. By hommaging his work from the Louis Vuitton 2015 F/W collection, Kim Jones is once again becoming a source of design inspiration in an era where upcycling fashion is attracting attention. Accordingly, an upcycling design approach might appeal to the MZ generation. Christopher Nemes' design presented in this study as a representative fashion designer of dismantling upcycling is basically based on dismantling. It can also be interpreted as dismantling upcycling design because it uses used clothing, waste clothing, and discarded fabrics as materials. This study examined main techniques of upcycling fashion design and derived characteristics of Nemes design as "deconstructive techniques using free lines of construction," "recycling upcycling vintage materials and clothing accessories," and "using handcrafted techniques of artistic sensibility." By applying Nemes' dismantling upcycling technique, four designs were developed for men's top and bottom sets for targets in their 20s and 30s to suggest the commercialization possibility. This study is meaningful in that it proposes a methodical alternative to the new deconstructionist upcycling design that can show Nemes' aesthetic characteristics, creative perspective, and personality by modernly reinterpreting the deconstructionist upcycling technique extracted through analysis of fashion designer Christopher Nemes' design. In particular, by actually producing the work and confirming whether the design technique can be implemented, the value as a fashion product that can be sold is realized.

Breeding of inbred lines 'Wongyo 3115' and 'Wongyo 3116' in Strawberry (딸기 근교계통 '원교3115호'와 '원교 3116호' 육성)

  • Jeong, Ho Jeong;Lee, Sun Hee;Cho, Il whan;Rho, Il Rae
    • Horticultural Science & Technology
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    • v.33 no.3
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    • pp.443-447
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    • 2015
  • Fragaria ${\times}$ ananassa (Duch.) cultivars, 'Wongyo 3 115' a nd 'Wongyo 3 116', we re bre d as a n inte rme diate parent l ine using heterosis of $F_1$ hybrids in octoploid strawberry. These two new cultivars were developed as inbred lines ($S_9$ generation) propagated from both seed and runners/stolons. 'Wongyo 3115', an inbred line derived from the 'Benihoppe' cultivar, has a semi-upright plant shape showing a vigorless growth habit, weaker than the original 'Benihoppe' cultivar; it has early flower bud differentiation and produces 10 flowers per cluster when grown from healthy seed. 'Wongyo 3115' has low yields of conical type fruit with pink colored skin; average fruit weight is approximately 11 g. The important characteristic of 'Wongyo 3115' is its excellent fruit firmness, firmer than the original variety. 'Wongyo 3116', an inbred line derived from the 'Doyonoka' cultivar, has an upright plant shape, vigorless growth habit, weaker than that of the original 'Doyonoka' cultivar; it has early flower bud differentiation and produces 8 flowers per cluster when grown from healthy seed. 'Wongyo 3 116' has oblate type fruits with red skin and an ave rage weight of approximately 12 g. The important characteristics of 'Wongyo 3116' are excellent fruit shape and higher yield than other inbred lines, although it produces small fruits compared to the original cultivar. Both cultivars have excellent taste, high sugar/acid ratio, and good texture. In regards to disease and pest resistance, 'Wongyo 3115' and 'Wongyo 3116' have a tendency to be sensitive to powdery mildew, anthracnose and the two-spotted spider mite.

Circular Dual Mode Horn Antenna(CDMHA) with Modified Aperture to Improve E/H-Plane Radiation Pattern Symmetry (E/H 평면 방사 패턴 대칭성 향상을 위해 개구면이 변형된 원형 이중 모드 혼 안테나)

  • Kim, Jae Sik;Yoon, Ji Hwan;Yoon, Young Joong;Lee, Woo-Sang;So, Joon-Ho
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.24 no.5
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    • pp.502-507
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    • 2013
  • In this paper, a circular dual mode horn with modified aperture is proposed to improve a E/H-plane radiation pattern symmetry of a conventional oversized circular dual mode horn. The proposed antenna consists of a feeding section, a mode generation section and a phase matching section which has aperture shape transition from circle to ellipse or rectangle to improve a E/H-plane radiation pattern symmetry. To compare the performances between the proposed antenna and the convenional circular dual mode horn, the conventional circular dual mode horn and the proposed circular dual mode horn with rectangular aperture are fabricated and researched at 15 GHz. The measured results show that the conventional circular dual mode horn has 3.394 dB difference while the proposed antenna has only 0.539 dB difference between E and H-plane radiation patterns within the -11 dB beamwidth($53^{\circ}$) which is required beamwidth of the feed horn for the maximum aperture efficiency where f/d ratio of reflector antenna is 1.

Atom-by-Atom Creation and Evaluation of Composite Nanomaterials at RT based on AFM

  • Morita, Seizo
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.73-75
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    • 2013
  • Atomic force microscopy (AFM) [1] can now not only image individual atoms but also construct atom letters using atom manipulation method [2]. Therefore, the AFM is the second generation atomic tool following the well-known scanning tunneling microscopy (STM). The AFM, however, has the advantages that it can image even insulating surfaces with atomic resolution and also measure the atomic force itself between the tip-apex outermost atom and the sample surface atom. Noting these advantages, we have been developing a novel bottom-up nanostructuring system, as shown in Fig. 1, based on the AFM. It can identify chemical species of individual atoms [3] and then manipulate selected atom species to the designed site one-by-one [2] to assemble complex nanostructures consisted of many atom species at room temperature (RT). In this invited talk, we will introduce our results toward atom-by-atom assembly of composite nanomaterials based on the AFM at RT. To identify chemical species, we developed the site-specific force spectroscopy at RT by compensating the thermal drift using the atom tracking. By converting the precise site-specific frequency shift curves, we obtained short-range force curves of selected Sn and Si atoms as shown in Fig. 2(a) and 2(b) [4]. Then using the atom-by-atom force spectroscopy at RT, we succeeded in chemical identification of intermixed three atom species in Pb/Sn/Si(111)-(${\surd}3$'${\surd}3$) surface as shown in Fig. 2(c) [3]. To create composite nanostructures, we found the lateral atom interchange phenomenon at RT, which enables us to exchange embedded heterogeneous atoms [2]. By combining this phenomenon with the modified vector scan, we constructed the atom letters "Sn" consisted of substitutional Sn adatoms embedded in Ge adatoms at RT as shown in Fig. 3(a)~(f) [2]. Besides, we found another kind of atom interchange phenomenon at RT that is the vertical atom interchange phenomenon, which directly interchanges the surface selected Sn atoms with the tip apex Si atoms [5]. This method is an advanced interchangeable single atom pen at RT. Then using this method, we created the atom letters "Si" consisted of substituted Si adatoms embedded in Sn adatoms at RT as shown in Fig. 4(a)~(f) [5]. In addition to the above results, we will introduce the simultaneous evaluation of the force and current at the atomic scale using the combined AFM/STM at RT.

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