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The Application of Rule of Mixtures to Fiber-Reinforced Composites(3) - Determination of Constant "a" and "b" for Modified Rule of Mixtures Applied to Fiber-Reinforced, Sulfur-Based Composites - (목재 섬유 복합재(複合材)에 혼합이론(混合理論)의 적용에 관한 연구(硏究)(3) - 유황(硫黃) 화합물(化合物)을 사용한 목재(木材) 섬유(纖維) 복합재(複合材)에 수정된 혼합이론(混合理論)의 상수(常數) 결정(決定) -)

  • Lee, Byung-G.
    • Journal of the Korean Wood Science and Technology
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    • v.12 no.3
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    • pp.3-8
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    • 1984
  • It is shown that Paul and Jones' Rule of Mixtures modified by Smith and Cox's theory can be used for the fiber-reinforced, sulfur-based composites, when the constant for the linear regression equation is given. The computation results, programmed by Hewlett Packard 75C (HP 75C) using math rom pack for the linear regression form, expressed as $E_c=\frac{1}{3}aE_fV_f+bE_mV_m$, turn out to be a=3.27-3.54 b=-2.47~-2.80. This results indicate that the factors such as density of fiber mat and the amount of matrix used have nothing for affecting the numerical value of the constants a and b of the linear regression form. Conclusively this results also show that the Paul and Jones' Rule of Mixtures which has been used for the composites made by randomly-oriented long fiber can also be used for the composites made by short fiber with the same fiber orientation such as wood and lignocellulosic fibers.

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Fabrication and Characterization of Organic Solar Cells with Gold Nanoparticles in PEDOT:PSS Hole Transport Layer (PEDOT:PSS 정공 수송층에 금 나노입자를 첨가한 유기태양전지의 제작 및 특성 연구)

  • Kim, Seung Ho;Choi, Jae Young;Chang, Ho Jung
    • Journal of the Microelectronics and Packaging Society
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    • v.20 no.2
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    • pp.39-46
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    • 2013
  • In this paper, organic solar cells(OSCs) based on bulk-heterojunction structures were fabricated by spin coating method using polymer P3HT and fullerene PCBM as a photoactive layer. The fabricated OSCs had a simple glass/ITO/PEDOT:PSS/P3HT:PCBM/Al structures. The photoactive layer of mixed P3HT:PCBM was formed with 1:1 weight ratio. The hole transport layer(HTL) was used conducting polymer PEDOT:PSS concentration with gold nanoparticles. The annealing temperature and concentration of nanoparticles in HTL were verified to improve the OSC characterization. The percentage of gold nanoparticles in HTL were 0.5 wt% and 1.0 wt%, and the surface morphology, electrical properties and absorption intensities were investigated. The devices were 0.5 wt%, and the highest 3.1% of the powder conversion efficiency(PCE), 10.2 $mA/cm^2$ of the maximum short circuit current density($J_{SC}$), 0.535V of the open circuit voltage($V_{OC}$) and 55.8% of the fill factor(F.F) could be obtained when the nanoparticle concertration was 0.5 wt%. The annealing temperature of HTL was $110^{\circ}C$, $130^{\circ}C$, $150^{\circ}C$ in vacuum oven and measured the absorption intensities, surface morphology, crystallinity and electrical properties were investigated. The best property was obtained in HTL annealed at $130^{\circ}C$ for gold nanoparticles of 0.5 wt%, showing that $J_{SC}$, $V_{OC}$, F.F and PCE were about 12.0 $mA/cm^2$, 0.525V, 64.2% and 4.0%, respectively.

Simultaneous Detection of Staphylococcus aureus, Salmonella enterica subsp., Vibrio parahaemolyticus by Multiplex Polymerase Chain Reaction (Multiplex Polymerase Chain Reaction(PCR)법을 이용한 Staphylococcus aureus, Salmonella enterica subsp., Vibrio parahaemolyticus의 다중동시검출)

  • Jeong, Yoo-Seok;Jung, Hee-Kyoung;Jeon, Won-Bae;Seo, Hwa-Jung;Hong, Joo-Heon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.4
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    • pp.595-601
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    • 2010
  • This study was conducted to detect and identify Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella enterica subsp. using simultaneous multiplex polymerase chain reaction (multiplex PCR) assay. 23S rRNA partial gene (S. aureus), tox R gene (V. parahaemolyticus), and inv A gene (S. enterica subsp.) as diagnostic marker gene were suggested, and their amplicon sizes were 482 bp, 368 bp, and 284 bp, respectively. Non specific amplicons by STA-5F/STA-5R primer, ToxR-F/ToxR-R primer, and 139/141 primer were not observed in genomic DNA of pathogen bacteria as Bacillus cereus, Listeria monocytogenes, Escherichia coli, Proteus vulgaris, Streptococcus pyogenes, Candida albicans, and Shigella sonnei. The extracted crude DNA of targeted bacteria was detected as PCR template successfully. The detection limits were $10^5\sim10^4$ CFU/mL and 10 pg of purified genomic DNA of S. aureus, V. parahaemolyticus, and S. enterica subsp. by using simultaneous multiplex PCR.

Verification of 2-Parameters Site Classification System and Site Coefficients (II) - Earthquake Records in Korea (2-매개변수 지반분류 방법 및 지반 증폭계수의 검증 (II) - 국내 실지진 기록을 통한 검증)

  • Lee, Sei-Hyun;Park, Dong-Hee;Ha, Jeong-Gon;Kim, Dong-Soo
    • Journal of the Korean Geotechnical Society
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    • v.28 no.3
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    • pp.35-43
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    • 2012
  • Following the companion paper (I. Comparisons with Well-known Seismic Code and Site Response Characteristics), several acceleration data recorded during recent earthquake events in Korea were analyzed to verify the suitability of the proposed two-parameters site classification system and the corresponding site coefficients. For all of rock-soil site pairs less than 30 km distant, response spectrums and corresponding site coefficients, $F_a$ and $F_v$, were determined. Unfortunately, some of data have an eccentric error, where the spectral acceleration of rock site is more amplified than that of soil site. The $F_a$ and $F_v$ for all of pairs except the pairs of error were compared with those in the current code and the proposed system. The $F_a$ and $F_v$ from the recorded motions show definitely different trend from that of the current code. In addition, the site coefficients from recorded motions at four 765 kV substation sites, which are several hundred meters distant, have a remarkably similar trend and absolute values to those in proposed two-parameters site classification system. Based on earthquake motions recorded in domestic areas including data from the four 765 kV substation sites, the two-parameters site classification and site coefficients are superior to the results obtained from the current Korean seismic code.

Increased DNA Polymerase Fidelity of the Lamivudine Resistant Variants of Human Hepatitis B Virus DNA Polymerase

  • Hong, Young-Bin;Choi, Yong-Wook;Jung, Gu-Hung
    • BMB Reports
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    • v.37 no.2
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    • pp.167-176
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    • 2004
  • Although efficient antiviral lamivudine is used for HBV-infected patients, a prolonged treatment with nucleoside analogs often results in lamivudine-resistant variants. In this study, we evaluated the fidelity of the lamivudine-resistant variants. The FLAG-tagged wild-type (FPolE) and Met550 variants (FPolE/M550A, M550V, and M550I) of HBV DNA polymerases were expressed in insect cells then purified. Like many other reverse transcriptases, no $3'{\rightarrow}5'$ exonuclease activity was detected in the HBV DNA polymerase. Since there is no proofreading activity, then the use of the site-specific nucleotide misincorporation method is beneficial. From the $f_{ins}$ value analysis, it is evident that M550I and M550V exhibit higher fidelity values than the wild-type HBV DNA polymerase, while M550A exhibits similar fidelity values. It is therefore suggested that lamivudine resistance comes from the stringency to dNTP binding and the discrimination of dCTP and lamivudine in M550V and M550I.

Electroporation Conditions for DNA Transfer into Somatic Embryogenic Cells of Zoysia japonica (들잔디 체세포 배발생 세포로의 DNA 전입을 위한 Electroporation 조건 구명)

  • 박건환;안병준
    • Korean Journal of Plant Tissue Culture
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    • v.25 no.1
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    • pp.13-19
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    • 1998
  • We have reported previously that intact embryogenic cells can be used instead of protoplasts for electroporation-mediated transformation of zoysiagrass and rice. In this study, conditions of the tissue electroporation were examined to optimize the procedures. Embryogenic cell suspensions were established in liquid MS medium containing 2 mg/L of 2,4-D with embryogenic calluses induced from mature embryos of Z. japonica. The suspension-cultured cell clumps were electroporated with 35S-gusA expression vector DNA, and degrees of DNA introduction into the cells were determined by histological expression rates of the gusA marker gene. DNA transfer into the cell clumps occurred in wide range of voltage (100-400 V) and capacitance (10-1980 $\mu\textrm{F}$), but more in the ranges of 200-300 V and 330-800 $\mu\textrm{F}$ DNA concentrations higher than 6 $\mu\textrm{g}$/mL were adequate for GUS expression of the electroporated cells. DNA transfers were confirmed in all three embryogenic cell lines but only in one out of eleven non-embryogenic lines. Positive GUS expressions occurred with DNAs added even 20-40 h after pulse treatments. As a promoter of gusA, Act1 and Ubi1 were effective 7 and 5 times than 35S respectively in number of GUS expression units on electroporated cell clumps. Embryogenic cell clumps survived and regenerated into plantlets after pulse treatments of wide range of conditions.

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A Study on Development of High Voltage Mica Capacitors (고전압 마이카 커패시터 개발에 관한 연구)

  • Yun, Eui-Jung;Choi, Cheal-Soon;Kim, Jae-Wook;Lee, Dong-Hyuk
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.57 no.7
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    • pp.1229-1234
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    • 2008
  • In this work, ultra high-voltage (17 - 50 kV AC), reliable 80 pF mica capacitors for partial discharge system application were investigated. Mica was used as the dielectric of the capacitors. Using the conservative design rule, over 3 individual $50\;{\mu}m$ thick mica sheets with a size of 30mm{\times}35mm were used with lead foils to form a parallel capacitor element and 20 mica sheets were interleaved with lead foils to form a series stack of parallel capacitor element to meet the requirements of the capacitors. The dimensions of the fabricated 80 pF capacitors for 17 kV AC and 50 kV AC were $90\;mm{\times}90\;mm$ and $95\;mm{\times}180\;mm$, respectively. The high-frequency characteristics of the capacitance (C) and dissipation factor (D) of the developed capacitors were measured using a capacitance meter. The developed capacitors exhibited C of 79.5 - 87.5 pF, had D of 0.001% over the frequency ranges of 150 kHz to 50 MHz, had a self-resonant frequency of 65 MHz, and showed results comparable to those measured for the capacitors prepared recently by $Adwel^{Tm}$. The developed capacitors also showed excellent characteristics for thermal shock test and temperature cycling test.

Effect of Shield Line on Noise Margin and Refresh Time of Planar DRAM Cell for Embedded Application

  • Lee, Jung-Hwan;Jeon, Seong-Do;Chang, Sung-Keun
    • ETRI Journal
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    • v.26 no.6
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    • pp.583-588
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    • 2004
  • In this paper we investigate the effect of a shield metal line inserted between adjacent bit lines on the refresh time and noise margin in a planar DRAM cell. The DRAM cell consists of an access transistor, which is biased to 2.5V during operation, and an NMOS capacitor having the capacitance of 10fF per unit cell and a cell size of $3.63{\mu}m^2$. We designed a 1Mb DRAM with an open bit-line structure. It appears that the refresh time is increased from 4.5 ms to 12 ms when the shield metal line is inserted. Also, it appears that no failure occurs when $V_{cc}$ is increased from 2.2 V to 3 V during a bump up test, while it fails at 2.8 V without a shield metal line. Raphael simulation reveals that the coupling noise between adjacent bit lines is reduced to 1/24 when a shield metal line is inserted, while total capacitance per bit line is increased only by 10%.

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Electrochemical Properties of $V_2O_5$ Electrodes as a Function of Additon of Carbon for Film Supercapacitor (Film형 Supercapacitor용 $V_2O_5$전극의 Carbon 첨가에 따른 전기화학적 특성)

  • Kim, Myung-San;Kim, Jong-Uk;Gu, Hal-Bon;Park, Bok-Kee
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2000.05b
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    • pp.39-41
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    • 2000
  • Carbon is an attractive candidate for use in eletrochemical supercapacitors that depend on charge storage in the electrode/eletorlyte international double layer. Property of an electrical double layer capacitor depend both on the technique used to prepare the electrode and on the current collector structure. The study is to research that $V_2O_5$-carbon (SP270) composite electrode for supercapacitor. The discharge capacitance of $V_2O_5$-SP270 (20wt%) in 1st and 35cyc1e was 14F/g and 8.5F/g at current density of $0.1mA/cm^2$. The discharge process of $V_2O_5$-SP270 (20wt%) composite electrode is larger than that others.

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Fruit Characteristics Based on Leaf to Fruit Ratio in 'Pione' Grapevine (Vitis vinifera × V. labrusca) during Cultivation with Heating (가온 재배 시 '피오네' 포도(Vitis vinifera × V. labrusca)의 엽과비에 따른 과실 특성)

  • Yun, Seok Kyu;Park, Seo Jun;Jung, Sung Min;Kim, Jung Bae;Yoon, Ik Koo;Nam, Eun Young;Yu, Duk Jun;Lee, Hee Jae
    • Korean Journal of Environmental Agriculture
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    • v.33 no.1
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    • pp.57-62
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    • 2014
  • BACKGROUND: Defoliation in grapevine cultivation is practically used to improve light environment within the canopy and thereby fruit quality. Effects of defoliation in five-year-old 'Pione' grapevine during cultivation with heating were investigated to find out optimum ratio of leaf area to fruit cluster weight (L/F). METHODS AND RESULTS: The grapevines were defoliated with berry-thinning 20 days after full bloom to provide various levels of L/F. At harvest, total leaf area values of fruit bearing branches were between 0.23 and $0.60m^2$. With increasing L/F, soluble solids and anthocyanin contents curvilinearly increased ($R^2=0.76^{**}$). At L/F over $0.6m^2/kg$, soluble solids content (SSC) leveled off. With increasing L/F, titratable acidity (TA) linearly decreased ($R^2=0.87^{**}$), but the ratio of SSC to TA linearly increased ($R^2=0.86^{**}$). Anthocyanin content was significantly correlated with SSC and the ratio of SSC to TA ($R^2=0.80^{**}$ and $0.82^{**}$, respectively). When total leaf area per fruit bearing branch was maintained $0.40m^2$, soluble solids and anthocyanin contents linearly decreased ($R^2=0.79^{**}$ and $0.85^{**}$, respectively), but TA linearly increased with increasing fruit cluster weight ($R^2=0.70^{**}$). Fruit was low in quality when the L/F was below $0.6m^2/kg$. CONCLUSION: L/F is recommended to be maintained at least $0.6m^2/kg$ in 'Pione' grapevine during cultivation with heating to produce higher-quality fruits.