• Title/Summary/Keyword: F-K 분석

Search Result 3,727, Processing Time 0.042 seconds

Direct detection of hemophilia B F9 gene mutation using multiplex PCR and conformation sensitive gel electrophoresis (Multiplex PCR과 Conformation Sensitive Gel Electrophoresis를 이용한 혈우병B F9 유전자 돌연변이 직접 진단법)

  • Yoo, Ki Young;Kim, Hee Jin;Lee, Kwang Chul
    • Clinical and Experimental Pediatrics
    • /
    • v.53 no.3
    • /
    • pp.397-407
    • /
    • 2010
  • Purpose : The F9 gene is known to be the causative gene for hemophilia B, but unfortunately the detection rate for restriction fragment length polymorphism-based linkage analysis is only 55.6%. Direct DNA sequencing can detect 98% of mutations, but this alternative procedure is very costly. Here, we conducted multiplex polymerase chain reactions (PCRs) and conformation sensitive gel electrophoresis (CSGE) to perform a screened DNA sequencing for the F9 gene, and we compared the results with direct sequencing in terms of accuracy, cost, simplicity, and time consumption. Methods : A total of 27 unrelated hemophilia B patients were enrolled. Direct DNA sequencing was performed for 27 patients by a separate institute, and multiplex PCR-CSGE screened sequencing was done in our laboratory. Results of the direct DNA sequencing were used as a reference, to which the results of the multiplex PCR-CSGE screened sequencing were compared. For the patients whose mutation was not detected by the 2 methods, multiplex ligation-dependent probe amplification (MLPA) was conducted. Results : With direct sequencing, the mutations could be identified from 26 patients (96.3%), whereas for multiplex PCRCSGE screened sequencing, the mutations could be detected in 23 (85.2%). One patient's mutation was identified by MLPA. A total of 21 different mutations were found among the 27 patients. Conclusion : Multiplex PCR-CSGE screened DNA sequencing detected 88.9% of mutations and reduced costs by 55.7% compared with direct DNA sequencing. However, it was more labor-intensive and time-consuming.

Occurrence of Fusarium Species in Korean Sorghum Grains (국내 수수 알곡에서의 Fusarium속 균의 발생현황)

  • Choi, Jung-Hye;Nah, Ju-Young;Jin, Hyun-Suk;Lim, Su-Bin;Paek, Ji-Seon;Lee, Mi-Jeong;Jang, Ja-Yeong;Lee, Theresa;Hong, Sung Kee;Choi, Hyo-Won;Kim, Jeomsoon
    • Research in Plant Disease
    • /
    • v.25 no.4
    • /
    • pp.213-219
    • /
    • 2019
  • A total of 1,159 Fusarium strains were isolated from sorghum grown in Danyang and Youngwol in 2017 and 2018. The isolates were analyzed to reveal genetic, toxigenic and pathogenic characteristics. Phylogenetic analysis using TEF-1α and RPB2 genes showed that the samples were contaminated with at least 17 Fusarium species. Among them, F. graminearum, F. proliferatum, F. thapsinum, F. incarnatum, and F. asiaticum were dominant species. In F. graminearum and F. asiaticum, F. graminearum-15-acetyl deoxynivalenol chemotype and F. asiaticum-nivalenol chemotype were frequent. Six Fusarium species tested produced one or more mycotoxins, except F. thapsinum and FTSC 11. F. proliferatum and F. fujikuroi had FUM1 gene (76.0% and 81.6%, respectively) and some isolates produced high level of fumonisin (over 1,000 ㎍). F. proliferatum and F. thapsinum were more virulent than other species on sorghum. These results indicate that Fusarium species in sorghum might produce multiple mycotoxins.

The Relationships of self-resilience, emotion expressiveness, achievement motivation, and problem-solving ability in nursing students (간호대학생의 자아탄력성, 정서표현성, 성취동기와 문제해결능력과의 관계)

  • Kim, Mi Young;Byun, Eun Kyung
    • The Journal of the Convergence on Culture Technology
    • /
    • v.6 no.2
    • /
    • pp.77-84
    • /
    • 2020
  • This study was to identify the relationship among self-resilience, emotion expressiveness, achievement motivation, and problem-solving ability of nursing students and provide data to increase problem-solving ability for nursing students based on the results. This research involved 229 nursing students in B city. The analyzed by the t-test, ANOVA, Pearson's correlation coefficient using SPSS/WIN 22.0. In results of the study, there were significant differences in problem-solving ability with respect to grade (F=4.021, p=.008), academic level (F=3.791, p=.024), satisfaction of major(F=11.140, p<.001), Accomplishment (F=8.000, p<.001), interpersonal relationship(F=16.944, p<.001). There were positive correlation between problem-solving ability and self-resilience(r=-.532, p<.001), between problem-solving ability and emotion expressiveness(r=.365, p<.001), between problem-solving ability and achievement motivation (r=.694, p<.001). Through this research requires the fellow study to determine the factors affecting problem-solving ability of nursing students.

Analysis of a wavelength tunable source according to temperature variations in a Mutually Injected F-P LD (상호주입 잠김 F-P LD에서 온도변화에 따른 가변 파장 광원의 특성 분석)

  • Hwang, Ji-hong;Oh, Yeong-guk;Lee, Hyuek-jae;Lee, Chang-hee
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
    • /
    • 2012.10a
    • /
    • pp.1009-1011
    • /
    • 2012
  • In this paper, a wavelength tunable light source based on mutually injected locking with two F-P LDs, has been constructed and then analyzed for wavelength shift and RIN (Relative Intensity noise) according to temperature. We have measured maximum about 2 nm for the wavelength shift and minimum -110dB/hz for the RIN. Also, the RIN and beating noise in eye patterns are increased by changing temperature high.

  • PDF

Scientific Analysis of National Registered Cultural Heritage 666, Korea's First Fighters used during the Korean War (F-51D) (등록문화재 제666호 F-51D 무스탕 전투기 과학적 분석)

  • Kang, Hyunsam;Jang, Hanul;Kim, Soochul;Lee, Uicheon
    • Conservation Science in Museum
    • /
    • v.23
    • /
    • pp.71-90
    • /
    • 2020
  • The scientific analysis of a 'F-51D Mustang Fighter'(Registered Cultural Heritage 666), one of the War Memorial of Korean collections, was carried out. The paint layer and canopy were discolored due to a constant outdoor exhibit. The results obtained through the scientific survey and analysis processes were intended to be used as basic data for the future dismantling and restoration of the fighter. The analysis results for the pigment components have confirmed red oxide of iron, Fe2O3 organic pigments, such as Cobalt Blue, phthalocyanine blue, etc., yellow PbCrO4, white TiO2, black Fe3O4, and gray Fe3O4 + TiO2. It has been also confirmed that Alkyd resin was mainly used for painting. The fighter's canopy was Poly methyl methacrylate(PMMA), and Al was detected as the main component of the fighter body, wings, and tails.

Catchment Similarity Assessment Based on Catchment Characteristics of GIS in Geum River Catchments, Korea (금강 유역을 대상으로 한 GIS 기반의 유역의 유사성 평가)

  • Lee, Hyo Sang;Park, Ki Soon;Jung, Sung Heuk;Choi, Seuk Keun
    • Journal of Korean Society for Geospatial Information Science
    • /
    • v.21 no.3
    • /
    • pp.37-46
    • /
    • 2013
  • Similarity measure of catchments is essential for regionalization studies, which provide in depth analysis in hydrological response and flood estimations at ungauged catchments. However, this similarity measure is often biased to the selected catchments and is not clearly explained in hydrological sense. This study applied a type of hydrological similarity distance measure-Flood Estimation Handbook to 25 Geum River catchments, Korea. Three Catchment Characteristics, Area(A)-Annual precipitation(SAAR)-SCS Curve Number(CN), are used in Euclidian distance measures. Furthermore, six index of Flow Duration Curve are applied to clustering analysis of SPSS. The catchments' grouping of hydrological similarity measures suggests three groups (H1, H2 and H3) and the four catchments are not grouped in this study. The clustering analysis of FDC provides four Groups; F1, F2, F3 and F4. The six catchments (out of seven) of H1 are grouped in F1, while Sangyeogyo is grouped in F2. The four catchments (out of six) of H2 are also grouped in F2, while Cheongju and Guryong are grouped in F1. The catchments of H3 are categorized in F1. The authors examine the results (H1, H2 and H3) of similarity measure based on catchment physical descriptors with results (F1 and F2) of clustering based on catchment hydrological response. The results of hydrological similarity measures are supported by clustering analysis of FDC. This study shows a potential of hydrological catchment similarity measures in Korea.

$F_2$-Monosomic Analysis of Plant Height in Triticum Cultivar Crosses Involving 'Diplomat' and 'Caribo' Monosomic Lines (밀의 초장결정 유전자분석)

  • Young-am, Chae
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.27 no.3
    • /
    • pp.235-237
    • /
    • 1982
  • $F_2$ progenies from the crosses between 'Caribo' monosomic lines and 'Diplomat' revealed significant differences in plant height in 7 $F_2$ populations. Well known effects of monosomic constritution of group 2 homoeologues and chromosome 5A sufficiently well explain reduced or increased plant height in these crosses respectively. Minor gene effects were recognized from 'Diplomat' chromosomes 3B, 6A and 7A and interpreted to explain or to contribute to genetic control of the rather small differences in plant height between cv. 'Caribo' and 'Diplomat'.

  • PDF

The Phase Noise prediction and the third PLL systems on 1/f Noise Modeling of Frequency Synthesizer (주파수합성기의 Phase Noise 예측 및 3차 PLL 시스템에서의 1/f Noise Modeling)

  • 조형래;성태경;김형도
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.5 no.4
    • /
    • pp.653-660
    • /
    • 2001
  • In this paper, we designed 2303.15MHz frequency synthesizer for the purpose of the phase noise prediction. For the modeling of phase noise generated in the designed system through introducing the noise-modeling method suggested by Lascari we analyzed a variation of phase noise as according as that of offset frequency. Especially, for the third-order system of the PLL among some kinds of phase noise generated from VCO we analyzed the aspect of 1/f-noise appearing troubles in the low frequency band. Since it is difficult to analyze mathematically 1/f-noise in the third-order system of the PLL, introducing the concept of pseudo-damping factor has made an ease of the access of the 1/f-noise variance. we showed a numerical formula of 1/f-noise variance in the third-order system of the PLL which is compared with that of 1/f-noise variance in the second-order system of the PLL. As a result, In case of txco we found the reduce rapidly along the offset frequency after passed through that phase-noise was -160dBc/Hz before passed through a loop at 10kHz offset frequency and -162.6705dBc/kHz after passed through the loop, -180dBc/Hz at 100kHz offset frequency and -560dBc/kHz after passed through the loop. We can notice that the variance of third-order system more occurs (or the variance of second-order system in connection with noise bandwidth and variance factor of second-order and third-order system.

  • PDF

Surface Analysis of Modified Polymer Samples by X-Ray Photoelectron Spectroscopy and Rutherford Backscattering Spectroscopy (X-선 광전자 분광법 및 라더포드 후방산란법에 의한 개질된 고분자 시료의 표면분석)

  • Park, Sung-Woo;Kim, Dong-Hwan;Kim, Young-Man;Park, Byung-Sun;Han, Wan-Soo;Suh, Bae-Suk
    • Analytical Science and Technology
    • /
    • v.7 no.3
    • /
    • pp.301-313
    • /
    • 1994
  • X-Ray Photoelectron Spectroscopy(XPS) and Rutherford Backscattering Spectroscopy(RBS) are used for the analysis of additives, examination of chemical structure and determination of identity with qualitative and quantitative analysis of surface elements, binding energy level and depth profiling in the surface. We analyzed surface of polyethylene, acrylonitrile butadien rubber, polypropylene, glass, fiber and paper treated with $XeF_2$ or C-F plasma by XPS and RBS. It was found that fluoro element was penetrated to sample surface and the distribution of surface elements are different than untreated samples.

  • PDF

Emotional Characteristics in MBTI Personality Type and MMPI-A Scale of Science Gifted (한국과학영재학생의 MBTI 성격유형과 MMPI-A 척도에서 나타난 정서적 특징)

  • Kwag, Mi-Yong;Park, Hoo-Hwi;Kim, Eel;Cheon, Seong-Moon;Sang, Wook
    • Journal of Gifted/Talented Education
    • /
    • v.20 no.3
    • /
    • pp.767-788
    • /
    • 2010
  • The purpose of this study was to examine emotional characteristics and to provide information about the special needs of counselling of science gifted in Korea. The subjects were 143 science gifted high school students in Busan that had been tested MBTI and MMPI-A. The distribution map of MBTI type was examined and Pearson's correlation, one-way ANOVA, multiple regression analysis were used to analyse the relation between MBTI and MMPI-A through SPSS 17.0 program. The results showed as follows: first, ENTP, INTP, ISTJ personality types and NT temperament type were the most frequently from the distribution map of MBTI type. Second, F1, F2, F, Hs, D, Pt, Sc and Si scales of MMPI-A were positively related to I preference of MBTI and K and Ma scales of MMPI-A were significantly related to E preference of MBTI from Pearson's correlation. Third, The score of IN group was significantly more high in F1, Hs, D, SC and Si scales of MMPI-A than other group in the relation between two combination preferences of MBTI and scale of MMPI-A. The following results were same; IS group in D, Si scales, EN group in Ma scale, IT group in Hs, D, Pt and S scales, IF group in VRIN, D and Si scales, ET in Ma scale, IJ group in D and Si, IP group in F1, F, Hs, D, Hy, Pt, Sc and Si scales, EJ and EP groups in Ma scale. Finally, I preference of MBTI by F1, F2, F, Hs, D, Pt, Sc and Si scales of MMPI-A, E preference of MBTI by Ma scale of MMPI-A, F preference of MBTI by K scale of MMPI-A and P preference of MBTI by Hy scale of MMPI-A were significantly predicted from multiple regression analysis. Limitations of the current study and the suggestions for further research were offered.