• Title/Summary/Keyword: F-K 분석

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Development of a SCAR Marker Linked to Male Fertility Traits in 'Jinkyool' (Citrus sunki) ('진귤' (Citrus sunki) 의 웅성가임 연관 SCAR 마커 개발)

  • Chae, Chi-Won;Dutt, Manjul;Yun, Su-Hyun;Park, Jae-Ho;Lee, Dong-Hoon
    • Journal of Life Science
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    • v.21 no.12
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    • pp.1659-1665
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    • 2011
  • In Citrus, an $F_1$ segregation population of 150 plants was constructed from a cross between 'Kiyomi' (C. unshiu ${\times}$ C. sinensis) carrying the male sterility trait and 'Jinkyool' (C. sunki). Sequence-related amplification polymorphism (SRAP) combined with bulked segregant analysis was used to develop markers linked to male fertility. In the $F_1$ population, 66 out of 150 seedlings had aborted anthers and the ratio of male sterile plants to fertile plants in the progenies matched the expected Mendelian segregation ratio of 1:1 ($x^2$ =2.16 at p=0.05). From the profiling of the 197 SRAP primer sets, three SRAP primer sets (F4/R27, F39/R60, and F15/R37) that were closely linked to the target trait were identified and successfully converted into a sequence characterized amplified region (SCAR) marker for selection of male fertility in citrus. The SCAR marker, using the pMS 33U/pMS 1462L primer set specifically, produced a single 1.4-Kb fragment that was linked to male fertility. Our results suggested that this SCAR marker can be useful for marker-assisted selection of male sterile individuals in breeding $F_1$ progenies in Citrus.

Effect of Defect Energy levels on the AC PDP Discharging Characteristics (MgO 보호막의 결함 전위 레벨이 AC-PDP 방전 특성에 미치는 효과)

  • Kwon, Sang-Jik;Kim, Yong-Jae;Cho, Eou-Sik
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.44 no.12
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    • pp.12-17
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    • 2007
  • The effects of the evaporation rate of MgO films using an electron beam on the MgO properties and the discharge characteristics of a plasma display panel(PDP) were investigated and analyzed. Mgo films were deposited with the various MgO evaporation rates. The MgO properties such as the crystal orientation, the surface roughness, and the film structure, were inspected using XRD(X-ray diffractometry), AFM(atomic force microscopy). From the experiments and Paschen law, the maximum value of the secondary, electron emission coefficient $(\gamma)$ was obtained at the evaporation rate of $5\AA/sec$. The minimum firing voltage and the maximum luminous efficiency were obtained at an evaporation rate of $5\AA/sec$. In the MgO film deposited at $5\AA/sec$, the (200) orientation and $F^+$ center were most intensive. The XRD results and cathode-luminescence(CL) spectra show the $\gamma$ values are correlated with $F/F^+$ centers of the molecular structure of MgO films.

Molecular Phylogenetic Study of Korean Hydrangea L. (한국산 수국속(Hydrangea L.) 식물의 분자 계통학적 연구)

  • Kim, Hye Sik;Park, Kyu Tae;Park, Seon Joo
    • Korean Journal of Plant Resources
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    • v.29 no.4
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    • pp.407-418
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    • 2016
  • In this study, the phylogenetic relationship of Korean Hydrangea was evaluated by using sequenced three chloroplast regions and ITS region, including the 7 taxa. The result of phylogenetic analysis indicated that Korean Hydrangea, 7 taxa formed the monophyletic group. This analysis also revealed that subsect. Macrophyllae of Korea was separated into two groups; H. serrata f. acuminate and H. macrophylla group. The H. serrata f. acuminta group was included with H. serrata f. buergeri and H. serrata f. fertilis. These three species form a monophyletic clade, with no significant differences between their nucleotide sequences. The H. serrata f. acuminta group showed a monophyletic group with H. serrata f. buergeri and H. serrata f. fertilis and there is significant differences between their nucleotide sequences. H. macrophylla group was an independent clade distinguished by H. serrate f. acuminate group. Subsect. Petalanthe, Heteromallae and Calyptranthae form a monophyletic group. H. petiolaris which is located in Subsect. Calyptranthae was separated into two subgroups; First subgroup: Jeju island (except for Mt. Halla) and Second subgroup: Ulleung island and Japan. Additional studies of two subgroups of H. petiolaris should be conducted a geographical study and add more samples.

The Effects of Learning Motivation Program for Freshmen of Nursing College: Focusing on Learning Motivation, Core Competence, Time Management, Career Attitude Maturity (간호대학 신입생의 학습동기유발 프로그램의 효과 분석: 학습동기, 핵심역량, 시간관리, 진로태도성숙을 중심으로)

  • Park, Ju-Young;Lim, Hyo-Nam;Kim, Doo Ree
    • Journal of the Korea Convergence Society
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    • v.9 no.8
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    • pp.331-341
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    • 2018
  • The purpose of this study was to develop a learning motivation program and to test its effect to provide basic data to be used for freshmen who are going to enter nursing college and various educational strategies and policies for successful university life. In order to develop the program, the contents of the program were structured so as to improve the learning ability, self-directed ability, and social competence through the current research and literature review. As a result, motivation (F=3.45 p=.033), core competence (F=7.35 p=.001), time management (F=9.80 p<.001) and career attitude maturity (F=19.83 p<.001) were significantly increased before the program. This suggests that the composition of the learning motivation program includes various learning strategies unlike nursing and majors.

Quantitative Evaluation of Leaching Model System for Soil Fungistasis (Leaching Model System에 의한 토양(土壤) 정균현상(靜菌現象)의 분석(分析))

  • Heo, Hyun;Hong, In-Pyo;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.15 no.4
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    • pp.254-259
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    • 1987
  • This study was carried out to investigate the germination of four fungi under non-leaching and leaching incubation. The range of germination rates of Fusarium solani f. sp. pisi, Fusarium oxysporum f. sp. cucumerium, Mortierella, and Helminthosporium sativa incubated on sand saturated with various solutions without leaching were 90-99%, 31-45%, 38-62% and 86-98% respectively. The range of germination rates of Fusarium solani f. sp. pisi, Fusarium oxysporum f. sp. cucumerium, Mortierella n. sp and Helminthosporum sativa incubated on sand undergoing leaching were 51-63%, 13-25%, 15-37% and 47-80%, respectively. The germination rates of the four fungi tested were suppressed under leaching incubation.

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A Study of Core Factor for Measure of Six Sigma Process (6시그마 측정(Measure) 단계의 핵심요인에 대한 실증적 연구;F사의 사례를 중심으로)

  • Choi, Moo-Ho;Lee, Kang-Koon
    • Proceedings of the Korean Society for Quality Management Conference
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    • 2006.11a
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    • pp.404-410
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    • 2006
  • 6시그마 프로세스는 정의(Define), 측정(Measure), 분석(Analyze), 개선(Improve), 관리(Control) 라는 다섯 Step을 통하여 문제를 해결하고 성과를 달성한다. 이 다섯 단계 중에서 측정 단계는 6시그마로는 길에서 매우 중요한 전이 단계로서 문제점을 정교하게 다듬고 근원적 원인 규명을 시작하는 데 도움을 준다. 즉 개선 하고자 하는 영역에 대한 사실적 근거를 제공 받기 위해 프로세스를 숫자로 전환 할 때 무엇을 측정하고 어떻게 측정 할 것인가를 결정한다. 측정단계는 주로 3 Step으로 이루어진다. Step 1 은 CTQ 특성치의 선정, Step 2는 성과기준의 정의, Step 3은 측정시스템의 분석이다. F사의 6시그마 발표자료 중 측정 부분만을 집중 분석하여 문제점 및 그 개선 방향을 제시하려한다.

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Genetic Analysis of Agronomic Characters in Interspecific Cross in Soybean (콩 종간교잡에서 주요형질의 유전분석)

  • Lee, Jeong-Dong;Kwon, Taek-Hwa;Cho, Ho-Young;Hwang, Young-Hyun
    • Current Research on Agriculture and Life Sciences
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    • v.20
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    • pp.1-8
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    • 2002
  • This study was conducted to obtain the information of varietal development using wild soybean through investigation of variation and heredity of major agronomic characters in $F_2$ generation of interspecific cross between Glycine max and G. soja. In segregating populations of two crosses, all characters observed except 100-seed weight showed transgressive segregation. Days to flowering showed normal distribution; mean days to flowering in $F_1$, and $F_2$ was approximately mean of parent. Mean of $F_1$ for 100-seed weight was 6.2g and 5.7g for Eunhakong/KLG10084 and Sohaegnamulkong/KLG10084, respectively, which were somewhat skewed distribution to small seeded parents. Mean seed yield of $F_2$ was about mean of two parents. Degree of vine was 6.4 and 5.7 in $F_1$ for two crosses but it was 6.0 in $F_2$ for Eonbakong/KLG10084, which revealed the degree of vine as partial dominance while 4.6 for Sobaegnamulkong/KLG10084, somewhat different results from the previous cross. Broad-sense heritability($h^2B$) for plant height, days to flowering, pods per plant, seed yield, and degree of vine was comparatively high and narrow-sense heritability($h^2B$) for 100-seed weight which is the moot important character in the development of small seed-size sprout soybean was 52.3% and 65.6% for Eunhakong/KLG10084 and Sohaegnamulkong/KLG10084, respectively, which indicated that selection for the character in early generation was possible.

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Sequence-to-sequence based Morphological Analysis and Part-Of-Speech Tagging for Korean Language with Convolutional Features (Sequence-to-sequence 기반 한국어 형태소 분석 및 품사 태깅)

  • Li, Jianri;Lee, EuiHyeon;Lee, Jong-Hyeok
    • Journal of KIISE
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    • v.44 no.1
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    • pp.57-62
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    • 2017
  • Traditional Korean morphological analysis and POS tagging methods usually consist of two steps: 1 Generat hypotheses of all possible combinations of morphemes for given input, 2 Perform POS tagging search optimal result. require additional resource dictionaries and step could error to the step. In this paper, we tried to solve this problem end-to-end fashion using sequence-to-sequence model convolutional features. Experiment results Sejong corpus sour approach achieved 97.15% F1-score on morpheme level, 95.33% and 60.62% precision on word and sentence level, respectively; s96.91% F1-score on morpheme level, 95.40% and 60.62% precision on word and sentence level, respectively.

Helicobacter pylori vacA Mosaicism and New Primers for vacA Signal Sequence Indigenous to Korea (Helicobacter pylori vacA 대립유전자의 Mosaicism과 Signal Sequence의 한국고유 시발체)

  • Ahn, Yeon-Hwa;Kim, Heung-Ryel;Lee, Ji-Eun;Hwang, Tae-Sook;Choe, Yon-Ho
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.4 no.2
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    • pp.155-160
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    • 2001
  • Purpose: Helicobacter pylori has been known to have diverse vacA allelic types. The purpose of the study was to identify vacA diversity in Korea and design new primers for signal sequence alleles indigenous to Korea. Methods: Fifty antral biopsy specimens, which had been proven to be H. pylori-positive, were examined for vacA status; signal sequence and mid-region. After PCR amplification and DNA sequencing, vacA alleles of Korean H. pylori strains were compared with those from other countries. Results: Among Korean H. pylori strains vacA alleles with all combinations of signal sequence and mid-region were found, with the exception of s1b or s2. vacA genotype s1c/m1 was predominant in Korea. We found that GGGAGCGTTR in s1a and GGGGYTATTG in s1c were the indigenous sequences to Korea and constructed the new Korean specific primers for the vacA signal sequence; VASK-F, VASK-R, S1AK-F, and S1CK-F. Conclusion: This study showed that s1c/m1 is the predominant type of vacA allele in Korea. We designed new primers for the vacA signal sequence.

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Analysis of Cyclic Adenosine Monophosphate (cAMP) Separation via RP-HPLC (reversed-phase high-performance liquid chromatography) by the Moment Method and the van Deemter Equation (역상 크로마토그래피에서 모멘트 방법과 van Deemter 식을 이용한 고리형 아데노신 일인산의 분리특성 연구)

  • Lee, Il Song;Ko, Kwan Young;Kim, In Ho
    • Korean Chemical Engineering Research
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    • v.53 no.6
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    • pp.723-729
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    • 2015
  • The moment analysis of cyclic adenosine monophosphate (cAMP) was performed using chromatograms that were obtained with the pulse input method from an octadecyl silica (ODS) high-performance liquid chromatography (HPLC) column. The general rate (GR) model was employed to calculate the first absolute moment and the second central moment. Three important coefficients for moment analysis, which are molecular diffusivity ($D_m$), external mass transfer coefficient ($k_f$), and intra-particle diffusivity ($D_e$), were estimated by the Wilke-Chang equation, Wilson-Geankoplis equation, and comparing van Deemter equation to theoretical plate number equation, respectively. Experiments were conducted by various conditions of flow rates, methanol volume ratio of the mobile phase, and solute concentration. After the moment analysis, results were organized by van Deemter plots. Also van Deemter coefficients were compared each other to effect $H_{ax}$, $H_f$, and $H_d$ on height equivalent to a theoretical plate (HETP, $H_{total}$). The value of intraparticle diffusion ($H_d$) was the primary factor which makes for HETP whereas external mass transfer ($H_f$) was disregardable factor.