• Korean Journal of Pharmacognosy
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• v.51 no.2
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• pp.93-99
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• 2020

This study was performed to find anti-inflammatory or antitumor compounds from the polar fraction obtained from the extract of Clinopodium chinense var. shibetchense (H. Lev) Koidz (Labiatae). Chromatography of the BuOH fraction yielded two flavonoid glycosides (compounds 1 and 2) and two saponins (compounds 3 and 4). On the basis of spectroscopic data, compounds 1 and 2 were identified to be ponciretin 7-O-α-L-rhamnopyranosyl-(1→6)-α-D-glucopyranoside (neoponcirin) and naringenin 7-O-α-L-rhamnopyranosyl-(1→6)-α-D-glucopyranoside (isonaringin). Compounds 3 and 4 were identified to be 3-O-{β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-fucopyranosyl}-saikogenin F (buddlejasaponin IV) and 3-O-{β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-fucopyranosyl}-21β-hydroxysaikogenin F (clinoposaponin XV). In addition, ursolic acid (5) was isolated and identified from the CHCl₃ fraction. Inducible nitric oxide synthase (iNOS) assay and sulforhodamine B (SRB) assay were performed to lead a potential anti-inflammatory or anti-tumor compounds from C. chinense var. shibetchense. Of the four compounds (1 - 4), compound 3 considerably inhibited cancer cell growth and NO production (IC₅₀s, 5.59 μM in iNOS assay and 6.62 - 14.88 μM in SRB assay).

• Proceedings of the Korean Society for Language and Information Conference
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• 2007.11a
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• pp.303-310
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• 2007

Named Entity Recognition (NER) is always limited by its lower recall resulting from the asymmetric data distribution where the NONE class dominates the entity classes. This paper presents an approach that exploits non-local information to improve the NER recall. Several kinds of non-local features encoding entity token occurrence, entity boundary and entity class are explored under Conditional Random Fields (CRFs) framework. Experiments on SIGHAN 2006 MSRA (CityU) corpus indicate that non-local features can effectively enhance the recall of the state-of-the-art NER systems. Incorporating the non-local features into the NER systems using local features alone, our best system achieves a 23.56% (25.26%) relative error reduction on the recall and 17.10% (11.36%) relative error reduction on the F1 score; the improved F1 score 89.38% (90.09%) is significantly superior to the best NER system with F1 of 86.51% (89.03%) participated in the closed track.

• BMB Reports
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• v.51 no.11
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• pp.572-577
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• 2018

Recent studies showed that the PD-1/PD-L1 checkpoint blockade is a dramatic therapy for melanoma by enhancing antitumor immune activity. Currently, major strategies for the PD-1/PD-L1 blockade have mainly focused on the use of antibodies and compounds. Seeking an alternative approach, others employ endogenous proteins as blocking agents. The extracellular domain of PD-1 (ePD1) includes the binding site with PD-L1. Accordingly, we constructed a PD-1-based recombinantly tailored fusion protein (dFv-ePD1) that consists of bivalent variable fragments (dFv) of an MMP-2/9-targeted antibody and ePD1. The melanoma-binding intensity and antitumor activity were also investigated. We found the intense and selective binding capability of the protein dFv-ePD1 to human melanoma specimens was confirmed by a tissue microarray. In addition, dFv-ePD1 significantly suppressed the migration and invasion of mouse melanoma B16-F1 cells, and displayed cytotoxicity to cancer cells in vitro. Notably, dFv-ePD1 significantly inhibited the growth of mouse melanoma B16-F1 tumor cells in mice and in vivo fluorescence imaging showed that dFv-ePD was gradually accumulated into the B16-F1 tumor. Also the B16-F1 tumor fluorescence intensity at the tumor site was stronger than that of dFv. This study indicates that the recombinant protein dFv-ePD1 has an intensive melanoma-binding capability and exerts potent therapeutic efficacy against melanoma. The novel format of the PD-L1-blocked agent may play an active role in antitumor immunotherapy.

• Journal of the Korean Mathematical Society
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• v.51 no.1
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• pp.87-98
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• 2014

Let R be a commutative ring with identity and M an R-module. In this paper, we associate a graph to M, say ${\Gamma}(M)$, such that when M = R, ${\Gamma}(M)$ is exactly the classic zero-divisor graph. Many well-known results by D. F. Anderson and P. S. Livingston, in [5], and by D. F. Anderson and S. B. Mulay, in [6], have been generalized for ${\Gamma}(M)$ in the present article. We show that ${\Gamma}(M)$ is connected with $diam({\Gamma}(M)){\leq}3$. We also show that for a reduced module M with $Z(M)^*{\neq}M{\backslash}\{0\}$, $gr({\Gamma}(M))={\infty}$ if and only if ${\Gamma}(M)$ is a star graph. Furthermore, we show that for a finitely generated semisimple R-module M such that its homogeneous components are simple, $x,y{\in}M{\backslash}\{0\}$ are adjacent if and only if $xR{\cap}yR=(0)$. Among other things, it is also observed that ${\Gamma}(M)={\emptyset}$ if and only if M is uniform, ann(M) is a radical ideal, and $Z(M)^*{\neq}M{\backslash}\{0\}$, if and only if ann(M) is prime and $Z(M)^*{\neq}M{\backslash}\{0\}$.

• Journal of Korean Ophthalmic Optics Society
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• v.20 no.1
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• pp.51-60
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• 2015

Purpose: To determine the effect of marketed multipurpose contact lens solutions (MPSs) on human corneal epithelial cells (HCEpiCs) toxicity by using image analysis. Methods: HCEpiCs were exposed six MPSs (product A-F) at 0.05~50% for 2h, 12h, 24h, and 48h respectively. HCEpiCs were fixed and stained with Draq5 after exposure with MPSs, and the cell viability and apoptosis were evaluated by using confocal microscope and ImageXpress UltraTM. Results: Viabilities of HCEpiCs exposed to MPS A-F for a 2h were not affected, while reductions (52~75%) in cell viability over a 12h exposure of MPS B, MPS C, MPS D and MPS F, and significant more reductions (29~73%) over a 24h and 48h-exposure. Apoptosis of HCEpiC was not affect over a 12h MPS exposure, however was significantly increased (199~526%) over 24h and 48h MPS exposure. Among the products MPS D, E and F reduced viability of HCEpiCs and apoptosis increased more than MPS A (p<0.05). Conclusions: Lower concentration of MPSs have not an cytotoxic effect on HCEpiCs, however higher concentration of MPSs induce apoptosis and reduce viability of HCEpiCs. Therefore, it need to develop MPS having antimicrobial effectiveness with low cytotoxicity.

• Mycobiology
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• v.51 no.3
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• pp.148-156
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• 2023

Penicillium oxalicum strain can be isolated from the Bletilla striata (Thunb.) Reichb. f. tubers. Its solid-state fermentation products are concentrated by percolation extraction. Separation and purification have been conducted to the ethyl acetate extracts by preparative HPLC. Based on the use of spectrometry, we have determined 17 known compounds, 12,13-dihydroxy-fumitremorgin C (1), pseurotin A (2), tyrosol (3), cyclo-(L-Pro-L-Val) (4), cis-4-hydroxy-8-O-methylmellein (5), uracil (6), cyclo-(L-Pro-L-Ala) (7), 1,2,3,4-tetrahydro-4-hydroxy-4-quinolin carboxylic acid (8), cyclo-(Gly-L-Pro) (9), 2'-deoxyuridine (10), 1-(b-D-ribofuranosyl)thymine (11), cyclo-(L-Val-Gly) (12), 2'-deoxythymidine (13), cyclo-(Gly-D-Phe) (14), cyclo-L-(4-hydroxyprolinyl)-D-leucine (15), cyclo-(L)-4-hydroxy-Pro-(L)-Phe (16), uridine (17). Here, we report compounds 1-3, 5, 7-8, 11-12, 14-17 are first found and isolated from this endophyte.

• Korean Journal Plant Pathology
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• v.1 no.1
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• pp.51-55
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• 1985

This study was carried out to observe the propagule of Fusarium moniliforme on the surface of rice seed and in the vascular bundle of rice stem by scanning electron microscope. Spore and mycelium of F. moniliforme were observed on the surface of rice seed and in the vascular bundle of rice stem. After seed treatment with Benlate T and Busan 3D, F. moniliforme was not isolated from chaffs, but frequently from brown rice, irrespective of disinfection period.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.1
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• pp.51-54
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• 2007

Soybean has a morphological type with a broadened and flattened stem. Fasciation has been suggested as a new gene for soybean research. SSR marker linked to the $\Large f$ locus that controls fasciation phenotype has not identified within 10 cM. A mapping population consisting of 94 $F_2$ progenies was derived from a cross between wild type Clark (FF) and fasciation mutant C32 (${\Large f}{\Large f}$). The phenotype of $F_2$ individual plants was recorded at R2 and R3 growth stage from field. One-thousand 10-mer oligonucleotide RAPD primers and 29 SSR primers selected from the D1b+W of the soybean molecular linkage map were used. A genetic map was constructed from the segregating 35 RAPD, four SSR markers and one phenotypic(wild type/fasciation) marker. The segregation ratios of 3 : 1 observed in the $F_2$ population and the Chi-square values strongly suggest that the fasciation trait is controlled by a single recessive gene. Satt537 marker was linked to $\Large f$ locus at a distance of 9.6 cM. Assignment of the $\Large f$ locus to linkage group D1b+W and identification of markers can be used as an initial step for fine mapping of the $\Large f$ gene.

• Journal of the Korean Society of Propulsion Engineers
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• v.5 no.3
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• pp.41-51
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• 2001

Combustion characteristics of a KSR-III liquid rocket engine with split-triplet (F-O-O-F) type injector elements are investigated numerically from the viewpoints of engine performance and combustion flowfield. To evaluate numerical analysis of liquid rocket engine with radial type injector arrangement, 2-D axisymmetric and 3-D calculations are carried out and the prediction of engine performance for design and off-design conditions is in a good agreement with hot-firing tests. According to 2-D axisymmetric and 3-D calculations, the prediction error is 3∼5 % from the standpoint of performance. Numerical results of combustion characteristics calculated through 3-D analysis agree well with hot-firing tests qualitatively at injector plate. Decreasing impinging angle and changing radial type injector arrangement to H type injector arrangement reduce effectively local high-temperature region. Also, it is examined that those affect the performance seriously. In conclusion, it is revealed that both injector arrangement and impinging angle are critical parameters to affect the performance and combustion characteristics of the liquid rocket engine.

• Journal of the Korean Chemical Society
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• v.51 no.5
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• pp.418-422
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• 2007

A simple and high yielding one-pot method for synthesis of 2,4,5-triarylimidazoles from condensation of benzoin or benzil, ammonium acetate and aromatic aldehydes using sulphanilic acid catalyst is described. The lower priced catalyst, higher yields and shorter reaction time are the advantages of the presented method.