• 시스템엔지니어링학술지
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• 제5권1호
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• pp.7-20
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• 2009

This paper describes a case study on the R&D programs of fighter and attacker aircraft such as F-22A, F/A-18E/F, and T/A-50. F-22A and F/A-18E/F were developed in same age. The performance of each program was, however extremely different. F-22A program results in a lot of cost overrun and schedule delay. On the other hand F/A-18E/F program met the cost, schedule, and performance goals. In the T/A-50 program with a super-sonic advanced trainer, T-50 was also developed successfully on planned cost and time by Korea Air-force and KAI. This paper derives key elements for the success of the military aircraft R&D program through lessons learned from th e case study. Each program is analyzed in terms of its background, planning and management.

• Asian-Australasian Journal of Animal Sciences
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• 제23권10호
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• pp.1268-1275
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• 2010

Post-weaning diarrhoea (PWD) and oedema disease (ED) caused by E. coli F18 always result in economic losses to pig producers, and no effective methods of controlling PWD and ED are presently available. FUT1 has been identified as a candidate gene controlling the expression of E. coli F18 receptor. This study examined the correlation between F18ab and F18ac adhesion phenotypes and the polymorphism at position M307 of the FUT1 gene in three pig breeds (231 Large White, 107 Landrace and 109 Songliao Black). The results showed: i) Both the susceptible genotypes (GG and GA) and the adhesion phenotypes (adhesive or weekly adhesive) were dominant in all three breeds with frequencies over 95%. ii) Three adhesion patterns of the two F18 variants F18ab and F18ac, i.e., ($ab^+$, $ac^+$), ($ab^+$, $ac^-$) and ($ab^-$, $ac^-$), were found in all three breeds, and there was no significant difference in the distribution of adhesion phenotypes of the two variants (separately or jointly) among the three breeds (p>0.05). iii) The FUT1 M307 genotypes were completely associated with the F18ab adhesion phenotypes and very strongly associated with the F18ac adhesion phenotypes. All individuals of genotype AA were non-adhesive to both F18ab and F18ac. All individuals of genotype GG or GA were adhesive to F18ab, whereas 11% of them were non-adhesive to F18ac. These results suggest that the polymorphism at FUT1 M307 can be used for marker-assisted selection of PWD and ED resistant pigs.

• Journal of Microbiology and Biotechnology
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• 제34권4호
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• pp.920-929
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• 2024

As a pivotal defensive line against multitudinous malignant tumors, natural killer (NK) cells exist in the tumor microenvironment (TME). RAD18 E3 Ubiquitin Protein Ligase (RAD18) has been reported to foster the malignant progression of multiple cancers, but its effect on NK function has not been mined. Here, the study was designed to mine the mechanism by which RAD18 regulates the killing effect of NK cells on colorectal cancer (CRC) cells. Expression of E2F Transcription Factor 7 (E2F7) and RAD18 in CRC tissues, their correlation, binding sites, and RAD18 enrichment pathway were analyzed by bioinformatics. Expression of E2F7 and RAD18 in cells was assayed by qRT-PCR and western blot. Dual-luciferase assay and chromatin immunoprecipitation (ChIP) assay verified the regulatory relationship between E2F7 and RAD18. CCK-8 assay was utilized to assay cell viability, colony formation assay to detect cell proliferation, lactate dehydrogenase (LDH) test to assay NK cell cytotoxicity, ELISA to assay levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ), and immunofluorescence to detect expression of toxic molecules perforin and granzyme B. High expression of RAD18 and E2F7 was found in CRC tissues and cells. Silencing RAD18 could hamper the proliferation of CRC cells, foster viability and cytotoxicity of NK cells, and increase the secretion of GM-CSF, TNF-α, IFN-γ as well as the expression of perforin and granzyme B. Additionally, ChIP and dual-luciferase reporter assay ascertained the binding relationship between RAD18 promoter region and E2F7. E2F7 could activate the transcription of RAD18, and silencing RAD18 reversed the inhibitory effect of E2F7 overexpression on NK cell killing. This work clarified the inhibitory effect of the E2F7/RAD18 axis on NK cell killing in CRC, and proffered a new direction for immunotherapy of CRC in targeted immune microenvironment.

• 생명과학회지
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• 제31권8호
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• pp.755-760
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• 2021

돼지 설사병은 부종병과 함께 양돈 농가에 막대한 경제적 손실을 입히는 가축질병으로 알려져 있으며, 병원성 대장균의 증식에 의하여 설사 및 대장균 생성 독소에 의한 부종병이 동반된다. 본 연구에서는 항균펩타이드를 포함하는 면역유도누에의 조추출물을 이용하여 돼지 설사 및 부종을 유발하는 F4+ 및 F18ab+ 대장균의 사균체를 제작하고 사균백신으로서 이용가능성을 검토하였다. 면역유도누에 추출물의 항균활성을 환확산법을 통해 분석한 결과 F4+ 및 F18ab+ 대장균에 대하여 높은 활성을 나타내었으며, 평판계수법을 이용하여 대장균의 사균체 생산효율을 분석한 결과 50 mg/ml의 농도를 4℃에서 처리했을 때 가장 높게 나타났다. 또한 제작된 사균체에 대해서 투과전자현미경을 이용하여 분석한 결과 세포질은 유출되고 세포막만이 남아있는 대장균이 확인되었다. 따라서 면역유도누에 추출물을 이용하여 제작된 F4+ 및 F18ab+ 대장균은 부종병 예방을 위한 사균백신으로서 이용가능성이 매우 높을 것으로 판단된다.

• 한국동물위생학회지
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• 제39권2호
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• pp.87-92
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• 2016

Porcine edema disease (ED) is a communicable disease of pigs caused by infection with Shiga toxin (Stx)-producing Escherichia coli (STEC) which expresses F18 fimbriae and/or Stx type 2e (Stx2e). While STEC causes a severe illness including hemorrhagic colitis and hemolytic-uremic syndrome in humans, it induces damage to the vascular endothelium, which results in edema, hemorrhage, and microthrombosis, leading in high mortality in pigs. In the present study, we cultured Stx2e-producing E. coli field isolates from conventional pig farms that experienced sudden deaths previously with symptoms similar to porcine edema disease, which were further investigated with Shiga toxin profiles. A total of 43 strains were identified from the collected samples by F18 or Stx2e specific PCR. Based on the PCR, 42 isolates out of 43 isolates were proved to carry one of F18 or Stx2e genes and 14 isolates to carry both F18 and Stx2e genes. All of the 30 isolates that harbored Stx2e gene induced the cytopathic effect (CPE) in vero cells and especially, the isolate 150229 produced the highest level of Shiga toxin. Therefore, we identified the virulence genes (F18 and Stx2e) and demonstrated Shiga toxin-producing abilities from porcine edema disease causing E. coli filed isolates. These results suggested that one of the isolates could be a vaccine antigen candidate against STEC through further investigating to elicit an immune response.

• 한국안전학회지
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• 제18권3호
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• pp.46-53
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• 2003

For the tensile tests of the F.E.M., microvoids are created by the boundary separation process at the martensite boundary or neighborhood and at inclusions within the fracture. to grow to the ductile dimple fracture. For the case of the M.E.F., microvoids created at the discontinuities of the martensite phase which exists at the grain boundary of the primary ferrite are grown to coalescence with the cleavage cracks induced at the interior of the ferrite, which as a result show the discontinuous brittle fracture behavior. In spite of their similar tensile strengths, the fatigue limit and the notch sensitivity of the M. E.F. is superior to those of the F.E.M., The M.E.F. is much more insensitive to notch than F.E.M. from the stress concentration factor($\alpha$).

• 충청수학회지
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• 제29권1호
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• pp.177-186
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• 2016

For a map $f:A{\rightarrow}X$, there are concepts of $H^f$-spaces, $T^f$-spaces, which are generalized ones of H-spaces [17,18]. In general, Any H-space is an $H^f$-space, any $H^f$-space is a $T^f$-space. For a principal fibration $E_k{\rightarrow}X$ induced by $k:X{\rightarrow}X^{\prime}$ from ${\epsilon}:PX^{\prime}{\rightarrow}X^{\prime}$, we obtain some sufficient conditions to having liftings $H^{\bar{f}}$-structures and $T^{\bar{f}}$-structures on $E_k$ of $H^f$-structures and $T^f$-structures on X respectively. We can also obtain some results about $H^f$-spaces and $T^f$-spaces in Postnikov systems for spaces, which are generalizations of Kahn's result about H-spaces.

• 한국전기전자재료학회논문지
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• 제18권4호
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• pp.375-380
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• 2005

CF₄ molecular gas is used in most of semiconductor manufacture processing and SF/sub 6/ molecular gas is widely used in industrial of insulation field. but both of gases have defect in global warming. C₃F/sub 8/ gas has large attachment cross-section more than these gases, moreover GWP, life-time and price of C₃F/sub 8/ gas is lower than them, therefor it is important to calculate transport coefficients of C₃F/sub 8/ gas like electron drift velocity, ionization coefficient, attachment coefficient, effective ionization coefficient and critical E/N. The aim of this study is to get these transport coefficients for imformation of the insulation strength and efficiency of etching process. In this paper, we calculated the electron drift velocity (W) in pure C₃F/sub 8/ molecular gas over the range of E/N=0.1∼250 Td at the temperature was 300 K and gas pressure was 1 Torr by the Boltzmann equation method. The results of this paper can be important data to present characteristic of gas for plasma etching and insulation, specially critical E/N is a data to evaluate insulation strength of a gas.

• Journal of the Korean Wood Science and Technology
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• 제8권1호
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• pp.22-27
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• 1980

Talysurf에 의한 톱니마모정도의 수량화가 수행되었다. (1)톱니의 단면은 아래식을 만족시키는 조건에서 Taysurf의 그래프로서 얻어진다. ${\frac{{\Delta}h}{h}}={\frac{V{\Delta}_x}{V_x}}$ {${\Delta}h$: stylus의 수직이동거리 h: 챠트에 있어서 수직거리 $V{\Delta}_x$: stylus의 이동속도 $V_x$: 챠트의 이동속도} (2) stylus의 오차는 아래식에 의하여 계산된다. i) 13.8${\mu}{\leqq}$x<20.4${\mu}$ y=-0.2246x+4.59${\mu}$ ii) 0${\leqq}$x<13.8${\mu}$ y=${\sqrt{(-18{\mu})^2-x^2}}-1.42x+32.7{\mu}}$ (3) 톱니단면과 stylus의 오차는 아래식에 의하여 계산된다. $E(%)=\frac{f(r){\times}{\frac{4}{18{\mu}}}}{f(R){\times}{\frac{R}{18.5{\mu}}}-f(r){\times}{\frac{r}{18{\mu}}}}{\times}100$ {E(%) : stylus의 오차/톱니의 둔함 r: stylus의 반경 R: 챠트에서 얻어지는 그라프의 반경 f(r): stylus의 오차 f(R): 챠트에서 얻어지는 그라프의 둔함} (4) 최대오차와 톱니단면의 관계에서 쌍곡선그라프를 얻을 수 있다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2010년도 추계 학술발표회 2차
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• pp.147-151
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• 2010

In this study, the clay specimen of Busan-Gyeongnam region was used for unconfined compression test to compare the relationship formula between elasticity modulus at peak($E_f$), elasticity modulus at $q_u$/2($E_{50}$), and cohesion when the sample breaks down by region and by level of cohesion. As the result, the regional results were found to be in the range of $E_f$ = 14c~47c and $E_{50}$ = 43c~137c; by cohesion, the results for very soft ground was $E_f$ = 15c~40c and $E_{50}$ = 54c~101c, $E_f$ = 13c~63c and $E_{50$ = 40c~147c for soft ground, $E_f$ = 18c~47c and $E_{50}$ = 57c~144c for medium ground, and $E_f$ = 25c~45c and $E_{50}$ = 68c~115c for solid ground. The average of the relationship formula between elasticity modulus-cohesion for the clay used in this study was $E_f$ = 32c, $E_{50}$ = 93c. This is 2.5~5 times smaller than the existing relationship formula.