• 농촌지도와개발
• /
• 제3권2호
• /
• pp.265-271
• /
• 1996

Locals of Control is considered one of the main imporment factors on responsible environmental behaviors(REB). It is defined as an individual's perception of his or ha ability to bring about change through his a her behavior. This psychological construct is divided in two, external and internal. Internal locus of control(ILOC) is imporment as predictor for responsible environmental behaviors. This study was conducted to investigate the Locus of Control and relations with REB in Undergraduate Students. The data were collected mainly through questionnaires and a total of 157 responded questionnaires was analyzed for the study. The statistical methods used in this study were frequency, percentile and correlation. The major findings of this study were (1) Most of undergraduate students had internal locus of control(75.2%). (2) According to region, rural students showed high levels in ILOC and REB.

• 한국균학회소식:학술대회논문집
• /
• 한국균학회 2015년도 추계학술대회 및 정기총회
• /
• pp.35-35
• /
• 2015

Pleurotus eryngii has recently become a major cultivated mushroom; it uses tetrapolar heterothallism as a part of its reproductive process. Sexual development progresses only when the A and B mating types are compatible. Such mating incompatibility occasionally limits the efficiency of breeding programs in which crossing within loci-shared strains or backcrossing strategies are employed. Therefore, understanding the mating system in edible mushroom fungi will help provide a short cut in the development of new strains. We isolated and identified pheromone and receptor genes in the B3 locus of P. eryngii and performed a functional analysis of the genes in the mating process by transformation. A genomic DNA library was constructed to map the entire mating-type locus. The B3 locus was found to contain four pheromone precursor genes and four receptor genes. Remarkably, receptor PESTE3.3.1 has just 34 amino acid residues in its C-terminal cytoplasmic region; therefore, it seems likely to be a receptor-like gene. Real-time quantitative RT-PCR (real-time qRT-PCR) revealed that most pheromone and receptor genes showed significantly higher expression in monokaryotic cells than dikaryotic cells. The pheromone genes PEphb3.1 and PEphb3.3 and the receptor gene PESTE3.3.1 were transformed into P5 (A3B4). The transformants were mated with a tester strain (A4B4), and the progeny showed clamp connections and a normal fruiting body, which indicates the proposed role of these genes in mating and fruiting processes. This result also confirms that PESTE3.3.1 is a receptor gene. In this study, we identified pheromone and receptor genes in the B3 locus of P. eryngii and found that some of those genes appear to play a role in the mating and fruiting processes. These results might help elucidate the mechanism of fruiting differentiation and improve breeding efficiency.

• Mycobiology
• /
• 제47권2호
• /
• pp.200-206
• /
• 2019

Allelic differences in A and B mating-type loci are a prerequisite for the progression of mating in the genus Pleurotus eryngii; thus, the crossing is hampered by this biological barrier in inbreeding. Molecular markers linked to mating types of P. eryngii KNR2312 were investigated with randomly amplified polymorphic DNA to enhance crossing efficiency. An A4-linked sequence was identified and used to find the adjacent genomic region with the entire motif of the A locus from a contig sequenced by PacBio. The sequence-characterized amplified region marker $7-2_{299}$ distinguished A4 mating-type monokaryons from KNR2312 and other strains. A BLAST search of flanked sequences revealed that the A4 locus had a general feature consisting of the putative HD1 and HD2 genes. Both putative HD transcription factors contain a homeodomain sequence and a nuclear localization sequence; however, valid dimerization motifs were found only in the HD1 protein. The ACAAT motif, which was reported to have relevance to sex determination, was found in the intergenic region. The SCAR marker could be applicable in the classification of mating types in the P. eryngii breeding program, and the A4 locus could be the basis for a multi-allele detection marker.

• 제어로봇시스템학회논문지
• /
• 제22권2호
• /
• pp.126-132
• /
• 2016

The root-locus method is often employed when a controller is designed to find controller gain. It is usually used to determine one parameter gain while most controllers for industrial applications have more than one controller gain. For example PID controller has three controller gains, i.e. P, I, and D gains. Thus the conventional root-locus technique cannot complete the design of a controller with more than one controller gain. One way to overcome this drawback has been to apply the root-locus technique for one parameter while other parameters are assumed to be proportional to the parameter or to be constant. However this approach could lead to limited performance of the controller and if we try to adjust the proportional ratio or constants then it could be a long and tedious process of trial and error. Thus it is required to find an effective method for the root-locus technique to design controllers with more than one parameter. To this end this paper proposes an extended root-locus method for controllers with two parameters. In this paper Matlab is used as a computation tool to show the effectiveness of our method by solving examples numerically. As a result we obtained an extended root-locus illustrated in two-dimensional space for a control system with two parameters. The paper then presents how to find two controller gains based on this result of the extended root-locus. The main idea is that we can find the parameters by approaching the desired poles. It is expected that the proposed idea will help control engineers to easily design control systems using the root-locus technique, resulting in more accurate and faster control systems. Note that the extended root-locus idea can be applied to controller design problems with multiple parameters.

• Journal of Microbiology and Biotechnology
• /
• 제22권9호
• /
• pp.1177-1184
• /
• 2012

In order to estimate how diverse the mating types in Pleurotus eryngii from different regions are, pairings between monokaryons derived from inter- and intra-groups were done. Sixteen and 15 alleles were identified at loci A and B from the 12 strains. In the P. eryngii KNR2312, widely used for commercial production, four mating loci, A3, A4, B3, and B4, were determined. Those loci, except A3, were found in 4 strains out of 12 strains. To improve breeding efficiency, especially in mating type determination, RAPD and BSA were performed to screen for a mating type specific marker. The SCAR marker 13-$2_{2100}$ was developed based on the RAPD-derived sequence typing B3 locus. The sequence analysis of 13-$2_{2100}$ revealed that it contained a conserved domain, the STE3 super-family, and consensus sequences like the TATA box and GC box. It seems likely that the SCAR marker region is a part of the pheromone receptor gene.

• 농촌지도와개발
• /
• 제27권4호
• /
• pp.159-171
• /
• 2020

This study aims to examine the needs analysis for agricultural management capabilities and farming practices that were emphasized by focusing to the inflow of farmers and the importance of the smooth succession. Data were collected from 134 farm successors. The Borich demand and the Locus for Focus model were hired for the study. Result were as follows. The competency group that the farm successors showed a high educational needs for was financial management. As for the detailed competencies that farm successors showed high educational needs for, based on Borich demand and the Locus for Focus model, the six detailed competencies of financial measures, financial analysis, financial management, sales planning, machine operation, and pest management were common and were derived as priorities. As for the direction of farming practice education for farm successors, based on these analysis results, education on machinery operation in the production sector should be expanded, and the management sector, more systematic and diverse education should be prepared to strengthen financial management capabilities.

• Clinical and Experimental Reproductive Medicine
• /
• 제23권1호
• /
• pp.109-114
• /
• 1996

General PCR technique alone has a limitation for preimplantation genetic diagnosis(PGD) using single blastomere. Recntly developed primer extension preamplification(PEP) technology amplifies the whole genome and thus, simultaneous multiple locus analysis became possible. In this study, we report the efficacy of PEP-PCR for PGD in three muscular dystrophy carriers undergoing IVF-ET. A total of 37 blastomeres were obtained from 40 embryos at six to eight cell stage in three IVF cycles in two DMD and one BMD carriers. Whole genome from single blastomeres were amplified using I5-base oligonucleotide random primers. PCR amplified products of exon 45 in the dystrophin gene and alphoid X/Y loci for gender determination were analysed by 2% metaphor gel electrophoresis. A total of 37 PEP-PCR replicates from 37 single blastomeres from 40 embryos and 37 blanks were performed. We obtained the reliable results for exon 45 and alphoid X/Y. Transfer of female embryos and unaffected male embryo was attempted in three couples. Unfortunately, pregnancy was not achieved in these cases. PEP-PCR is a reliable and efficient PGD method in multiple locus analysis using single blastomere.

• 산업경영시스템학회지
• /
• 제40권4호
• /
• pp.171-182
• /
• 2017

Most people seek a happy life and happiness positively affects sentiment, satisfaction with life, creativity, human relationship, business productivity, and even health and life extension. However, according to a survey in 2013, subjective happiness of adolescents (including university students) was very low compared to other age groups in Korea. Therefore this paper examined the effects of job-seeking stress, appearance recognition, financial situation, trust in government, and locus of control on university students' happiness using SEM (structural equation modeling). 207 university students in Seoul, Korea have been surveyed. At first, an initial experimental SEM model among these variables has been set up and reliability analysis has been conducted. Then multiple regression analyses on job-seeking stress and happiness as well as SEM analysis have been conducted. As a result of these analyses, the SEM model has been revised two times. The final SEM model passed the goodness-of-fit test (using RMR, GFI, NFI, CFI, and IFI indices). The final SEM model showed the followings. First, Higher job-seeking stress (especially sentimental part, rather than environment or action related parts) negatively affects happiness. Second, Trust in government also affects happiness both directly and indirectly. Third, Locus of control is affected both by trust in government and financial situation. Fourth, appearance recognition heavily affects job-seeking stress. In addition, appearance importance is higher than appearance interest, meaning that students who are not very interested in appearance usually recognize the importance of appearance. Finally, happiness is affected neither financial situation nor appearance recognition. Therefore, even either they are in a poor financial situation or not happy with their appearance, they can be happy if they have firm locus of control.

• 대한의용생체공학회:의공학회지
• /
• 제16권2호
• /
• pp.217-222
• /
• 1995

We propose some applications of image processing techniques to extract quantitative measurements by using a camera system developed in Korea university and Catholic Medical School. From now on the system will be called as KCMOTION. The purpose of this study is to provide basic kinematic and kinetic data for the analysis of human movements and to find the clinical usefulness and reliability of the proposed motion analysis system. Two tests, sit-to-stand (STS) movements and pendulum test, are conducted by the system. The aims of the tests are to identify variability and reliability of KCMOTION to give some quantitative comparisons to the other systems. The result of STS movement are compared to the LOCUS IIID motion analyzer by the ratio of maximum flexion movement per body weight to the actual maximum flexion extension torque per body weight. That result in 29 % and 33 % for hip and knee joint, respectively in KCMOTION and 27 % and 30 % in LOCUS IIID System. The results of the pendulum movements are compared to that of using Cybex and Electrogoniometer with relaxation index, amplitude ratio, swing number and swing time. The results of relaxation index and amplitude ratio of the KCMOTION are between those of the Cybex and Electrogoniometer. We also observed that the KCMOTION detect more natural movement, from the results of swing number and time.

• 농촌지도와개발
• /
• 제29권4호
• /
• pp.203-224
• /
• 2022

This study aims to examine the educational needs of migrant farm workers and farmers in Korea. For developing survey instruments, content analysis of migrant workers & employer training programs, in-depth interviews with farmers and expert reviews had been conducted. Therefore, eight parts and twenty nine educational contents for migrant workers, five parts and twenty educational contents for farmers have been constructed. For data analysis, Borich analysis and The Locus for Focus Model were hired to analyze the educational needs assessment. Results showed that migrant workers' educational priorities were 'basic korean for farming, working attitude in farming, safety guidelines and first aid skills for rural life, safe use of pesticides and protective equipment, driving skills, safe use of agricultural machinery, operation & maintenance of agricultural machinery, pest control by crop', and for the farmers' were 'communication with migrant workers, trouble controls with employment, farming guidance, industrial accident cases and countermeasures, preventing disease and promoting mental health of migrant workers'.