• Geomechanics and Engineering
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• 제28권3호
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• pp.299-311
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• 2022

Water-resisting key stratum (WKS) between coal seams is an important barrier that prevents water inrush from goaf in roof under multi-seam mining. The occurrence of water inrush can be evaluated effectively by analyzing the fracture of WKS in multi-seam mining. A "long beam" water inrush mechanical model was established using the multi-seam mining of No. 2+3 and No. 8 coal seams in Xiqu Mine as the research basis. The model comprehensively considers the pressure from goaf, the gravity of overburden rock, the gravity of accumulated water, and the constraint conditions. The stress distribution expression of the WKS was obtained under different mining distances in No. 8 coal seam. The criterion of breakage at any point of the WKS was obtained by introducing linear Mohr strength theory. By using the mechanical model, the fracture of the WKS in Xiqu Mine was examined and its breaking position was calculated. And the risk of water inrush was also evaluated. Moreover, breaking process of the WKS was reproduced with Flac3D numerical software, and was analyzed with on-site microseismic monitoring data. The results showed that when the coal face of No. 8 coal seam in Xiqu Mine advances to about 80 m ~ 100 m, the WKS is stretched and broken at the position of 60 m ~ 70 m away from the open-off cut, increasing the risk of water inrush from goaf in roof. This finding matched the result of microseismic analysis, confirming the reliability of the water inrush mechanical model. This study therefore provides a theoretical basis for the prevention of water inrush from goaf in roof in Xiqu Mine. It also provides a method for evaluating and monitoring water inrush from goaf in roof.

• 한국엔터테인먼트산업학회논문지
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• 제14권6호
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• pp.43-52
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• 2020

본 논문은 구스타프 도이치(Gustav Deutsch) 감독의 영화 <셜리에 관한 모든 것>(2013)에 대한 연구이다. 이 영화는 화가 에드워드 호퍼(Edward Hopper)의 회화를 오마주 해 영화로 변환하였는데. 회화 작품을 있는 그대로 활용하면서 움직임을 가미였기 때문에 마치 그림이 움직이는 것 같은 효과를 준다. 그런 점에서 영화와 회화간의 재매개라는 문제를 제기하고 있다. 본 연구에서는 <셜리에 관한 모든 것>이 호퍼의 그림을 영화로 전환함에 있어 어떤 방식으로 움직임을 다루었는가를 배우의 움직임과 화면의 움직임이라는 두 측면에서 살펴본다. 조르조 아감벤의 몸짓(gesture) 개념이 이 과정에서 분석의 틀로 사용되었다. 이 영화에서 배우의 움직임은 사건을 만들고 전개하는 일반적인 액팅(action)이라기보다는 인물의 동작과 표정 그 자체를 드러내는 몸짓이다. 스토리를 목적으로 하는 연기가 아닌 조르조 아감벤이 영화의 가능성으로 말했던 물화된 이미지를 해방하는 몸짓인 것이다. 편집과 카메라 움직임은 정면성을 유지한 채 사용된다. 이는 화면의 움직임이 관객 시선임을 말해주며 언뜻 보기엔 원작의 관음증적 시선을 구현하고 있다. 하지만 관객은 주류 허구 영화에서와 같이 이미지 대상을 일방적으로 보는 것이 아니라 자신 또한 보여지고 있다. 또한 화면의 디테일을 자세히 보려는 카메라 움직임은 세부를 드러내는 수단이라기보다는 움직임 그 자체를 보여주고 있다. 영화의 현실감은 움직임을 통해서 만들어진다. 이영화는 회화와 영화 사이에서, 말과 이미지 사이에서 현실의 비전을 질문하고 있다. 움직임은 현실을 매개하는 수단이지만, 이 영화는 그 매개성을 드러냄으로써 아감벤이 말했던 잃어버린 몸짓을 다시 되찾고 있다. 이는 현실의 비전이 그러한 매개성을 가렸을 때 출현함을 말해준다.

• 한국엔터테인먼트산업학회논문지
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• 제14권1호
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• pp.117-124
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• 2020

중국 청년 영화는 2013-2017년의 빠른 발전을 거친 후 지속적인 발전에 일정한 난관에 부딪히게 되었다. 따라서 영화의 창작 이념과 방법의 혁신을 통한 관객들의 감상 취미에 대한 소환과 새로운 심미 체험의 촉발은 중국 영화 시장에 관심을 가진 창작자들이 직면한 문제가 되었다. 영화 <먼 훗날 우리>는 2018년 큰 성공을 거둔 청년 영화이며, 그 창작 실천의 낯설게 하기는 중국 청년 영화의 창작에 새로운 이론과 방법 근거를 제공할 수 있다는 사실을 증명하였다. 따라서 중국 청년 영화 발전 상황을 토대로 영화 <먼 훗날 우리>를 연구 대상으로 삼아 서사담론 와 서사내용 등 두 가지 측면에서 영화의 낯설게 하기 표현 방법에 대해 분석하였다. 먼저 화면, 인물, 플롯은 서사담론의 낯설게 하기를 구성해 영화가 현실 세계를 "돋보이게"하고, 그 다음 도시 콤플렉스, 정체성, 고향 정서 등이 서사내용의 낯설게 하기를 구성해 기존 패턴에서 영화를 "돋보이게"한다. 이러한 창작 방법은 당대의 수요를 만족시킬 수 있을 뿐만 아니라 기타 청년 영화 창작에 효과적인 참고를 제공할 수 있다. 비록 시대가 변화되고 있지만 성공 사례에 대한 분석을 통해 낯설게 하기 요소를 어떻게 이용할 것인지에 대한 연구는 여전히 매우 필요한 부분이다.

• 한국버섯학회지
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• 제20권4호
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• pp.241-248
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• 2022

생육온도가 느타리류 품종의 갓색과 수량, 형태관련 형질에 어떤 영향을 미치는지 알아보고자 하였다. 주요결과는 다음과 같다. 느타리류의 모든 품종에서 생육온도가 12℃에서 21℃로 높아짐에 따라 갓색의 명도(L)가 높아졌지만, 산타리, 황금산타리, 순정은 21℃의 갓색 명도가 18℃ 보다 낮았다. 갓색의 적색도와 황색도는 생육온도가 높아 질수록 낮아졌는데, 유채색계열의 변화폭이 컸다. 유채색의 황색도는 생육온도 전반에서 무채색보다 높았다. 전체 수량은 생육온도가 높아질수록 많아지는 경향이었으나, 저온성 품종인 원형1호는 저온일수록 전체수량이 높았고 21℃에서는 발이가 불가능하였다. 유효경수는 대체적으로 18℃에서 가장 많았고, 수량과의 상관관계는 낮았다. 자실체의 전체 길이와 대두께는 생육온도의 상승에 따라 길어지고 뚜꺼워지는 경향이 뚜렸했으며, 흑타리의 전체길이와 온도사이의 결정계수(R²)는 0.514, 산타리의 대두께와 온도사이의 결정계수는 0.963로 나타났다. 상관분석의 결과 한 형질에 대해 복수의 형질이 높은 상관관계를 보였다. 앞으로 RNA 발현 분석을 통하여 생육온도에 따라 변화하는 느타리류 갓색물질과 관련 있는 유전자의 변화 양상을 밝힐 계획이다.

• 대한소아치과학회지
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• 제49권3호
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• pp.253-263
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• 2022

협측소와는 하악 대구치 발달구의 말단에 나타나는 강한 점상의 함몰된 구조이다. 협측소와 결함은 협측소와가 상아질까지 침범한 상태로 정의할 수 있다. 이 연구는 콘빔 컴퓨터 단층촬영(CBCT) 분석을 통해 소아청소년의 하악 제1대구치 및 제2 대구치에서 협측소와 및 협측소와 결함의 발생 빈도를 조사하는 것을 목적으로 하였다. 연구는 2004년부터 2020년까지 연세대학교 치과병원 소아치과를 방문한 한국 소아청소년 417명을 대상으로 촬영한 CBCT 영상을 대상으로 수행하였다. 협측소와의 발현율은 29.1%로 보고되었다. 제4급 협측소와에 해당하는 협측소와 결함의 유병률은 총 7.9%였다. 협측소와는 양측성으로 나타나는 경향이 있었다. 이 연구는 협측소와의 빈도에 대해 가장 큰 표본 크기를 사용하여 시행한 포괄적인 연구이며, CBCT를 이용한 최초의 연구였다. 또한 협측소와 결함을 최초로 정의 내리고자 하였다.

• Nutrition Research and Practice
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• 제16권6호
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• pp.685-699
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• 2022

BACKGROUND/OBJECTIVES: Platycodon grandiflorum (PG) has long been known as a medicinal herb effective in various diseases, including bronchitis and asthma, but is still more widely used for food. Fermentation methods are being applied to increase the pharmacological composition of PG extracts and commercialize them with high added value. This study examines the hydrolyzed and fermented PG extract (HFPGE) fermented with Lactobacillus casei in RAW 264.7 cells, and investigates the effect of amplifying the immune and the probable molecular mechanism. MATERIALS/METHODS: HFPGE's total phenolic, flavonoid, saponin, and platycodin D contents were analyzed by colorimetric analysis or high-performance liquid chromatography. Cell viability was measured by the MTT assay. Phagocytic activity was analyzed by a phagocytosis assay kit, nitric oxide (NO) production by a Griess reagent system, and cytokines by enzyme-linked immunosorbent assay kits. The mRNA expressions of inducible nitric oxide synthase (iNOS) and cytokines were analyzed by reverse transcription-polymerase chain reaction, whereas MAPK and nuclear factor (NF)-κB activation were analyzed by Western blots. RESULTS: Compared to PGE, HFPGE was determined to contain 13.76 times and 6.69 times higher contents of crude saponin and platycodin D, respectively. HFPGE promoted cell proliferation and phagocytosis in RAW 264.7 cells and regulated the NO production and iNOS expression. Treatment with HFPGE also resulted in increased production of interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, C-X-C motif chemokine ligand10, granulocyte-colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, and monocyte chemoattractant protein-1, and the mRNA expressions of these cytokines. HFPGE also resulted in significantly increasing the phosphorylation of NF-κB p65, extracellular signal-regulated kinase, and c-Jun N-terminal kinase. CONCLUSIONS: Taken together, our results imply that fermentation and hydrolysis result in the extraction of more active ingredients of PG. Furthermore, we determined that HFPGE exerts immunostimulatory activity via the MAPK and NF-κB signaling pathways.

• 대한본초학회지
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• 제29권4호
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• pp.53-59
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• 2014

Objectives : The purpose of this study was verification of the anti-inflammation and anti-oxidant effect of Cheongungdajosan-gamibang extract (CG) in mouse macrophage, RAW 264.7 cells. Methods : We have basically using LPS-stimulated RAW 264.7 cells. The cell toxicity was determined by MTT assay. To evaluate the anti-inflammatory effect of Cheongungdajosan-gamibang, amount of nitric oxide(NO) was measured using the NO detection kit and the IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ expression was measured by reverse transcriptase polymerase chain reaction (RT-PCR). Also, free radical scavenging assay has tested for DPPH and ABTS radical activity as well as the contents of total polyphenol. Results : In this study, 96.6% or higher cell viability was observed in all tested groups from 1, 10, $100{\mu}/m{\ell}$ in RAW 264.7 cells. The RAW 264.7 cells were induced by lipopolysaccharide (LPS) and CG 1, 10, $100{\mu}/m{\ell}$. The CG decreased nitric oxide (NO) production activity dose dependently, especially at $100{\mu}/m{\ell}$ of 55%. The production of IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ were decreased by 51%, 78% and 35% in CG treated $100{\mu}g/m{\ell}$. CG showed dose-dependent suppression activity of reactive oxygen species (ROS) production, especially at $100{\mu}g/m{\ell}$ of 37%. DPPH radical scavenging activity and ABTS cation decolorization were activated over 86% and 88% in CG at $1,000{\mu}g/m{\ell}$ concentration. Conclusions : According to the results, we thought that CG showed anti-inflammatory and antioxidant activities on the RAW 264.7 cells in mouse macrophage. Therefore, this research is expected to provide the fundamental data about the natural material analysis of relating to the anti-inflammation and antioxidant.

• 대한본초학회지
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• 제28권5호
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• pp.7-11
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• 2013

Objectives : Seungmagalgeun-tang (SMGGT) is traditional medicine widely used for inflammatory disease and flu. But SMGGT exhibits potent anti-inflammatory activity with an unknown mechanism. To elucidate the molecular mechanisms of SMGGT water extract on pharmacological and biochemical actions in inflammation, we examined the effect of SMGGT on pro-inflammatory mediators in Phorbol-12-myristate-13-acetate (PMA)+A23187-stimulated mast cells. Methods : In the present study, pro-inflammatory cytokine production was determined by performing enzyme-linked immunosorbent assay (ELISA), reverse transcription polymerase chain reaction (RT-PCR), and western blot analysis to measure the activation of MAPKs. Cells were treated with SMGGT 1 h prior to the addition of 50 nM of PMA and $1{\mu}M$ of A23187. Cell viability was measured by MTS assay. The investigation focused on whether SMGGT inhibited the expressions of interleukin-6 (IL-6), interleukin-8 (IL-8) and mitogen-activated protein kinases (MAPKs) in PMA+A23187-stimulated mast cells. Results : SMGGT has no cytotoxicity at examined concentration (100, 250, and $500{\mu}g/ml$). Also, gene expression of IL-6 and IL-8 in HMC-1 cells stimulated by PMA+A23187 was down regulated by SMGGT. Furthermore, SMGGT suppressed the PMA+A23187-induced phosphorylation of extracellular signal-regulated kinase (ERK) and c-jun N-terminal Kinase(JNK). But, SMGGT could not regulate phosphorylation of p38 MAPK. Conclusions : These results suggest that SMGGT has inhibitory effects on PMA+A23187-induced IL-6 and IL-8 production. These inhibitory effects occur through blockades on the phosphorylation of ERK and JNK.

• 대한본초학회지
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• 제32권2호
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• pp.25-32
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• 2017

Objectives : This study was conducted to investigate candidate materials as anti-inflammatory agent from extracts of Korean medicinal plants in Hwaak mountain. Ligustrum obtusifolium (LO) is a Korea medicinal plants that commonly used for robustness and hemostasis. It has been reported that LO has exhibited anti-ischemic, anti-oxidative, anti-hypolipidemic, anti-tumor and hypoglycemic effects. However, LO has not been previously reported to have an anti-inflammatory effect. Therefore, we have evaluated the anti-inflammatory effects of LO and its underlying molecular mechanisms in LPS-induced RAW 264.7 macrophages. Methods : Cell viability was determined by MTT assay in RAW 264.7 macrophages. Nitric Oxide (NO) was measured with Griess reagent and pro-inflammatory cytokines were detected by ELISA in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Protein expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and p65 subunit of nuclear $factor-{\kappa}B$ ($NF-{\kappa}B$) were determined by Western blot analysis. Results : Among 15 extracts of Korean medicinal plants tested, Ligustrum obtusifolium (LO) showed the inhibition of NO production without cytotoxicity. LO reduced the expression levels of iNOS and COX-2 proteins in LPS-simulated RAW 264.7 macrophages in dose-dependent manner. Consistent with these data, LO inhibited the productions of $TNF-{\alpha}$, IL-6, and $IL-1{\beta}$ in LPS-simulated RAW 264.7 macrophages. Furthermore, LO attenuated the LPS-induced nuclear translocation of p65 $NF-{\kappa}B$ in RAW 264.7 macrophages involving suppression of $NF-{\kappa}B$ activation. Conclusions : Taken together, these results suggest that the anti-inflammatory effects of LO is associated with regulation of inflammatory mediators via inhibition of $NF-{\kappa}B$ activation in LPS-treated RAW 264.7 macrophages.

• 대한본초학회지
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• 제33권4호
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• pp.19-26
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• 2018

Objectives : The aim of this study was to investigate the immunopotentiating activity of combine extract that Silkworm and Cinnamomum cassia. Recently, acute epidemic diseases such as cold and viral respiratory diseases have been emerging. So, interested in immunity enhancement has been increasing, and research on natural products to promote immunity activity has been actively conducted. Methods : To confirm the immunopotentiating activity effect, Silkworm (SW), Cinnamomum cassia (CC), and SWCC combined extracts were treated 14 days at 300 mg/kg/day. The changes of glutamic oxalacetic transaminase (GOT), glutamic pyruvate transaminase (GPT) in serum were analyzed after experiment. The changes in the total spleen cell number were measured. Immune cells in spleen were analyzed using fluorescence activated cell sorter (FACS). also, analyzed the expression of cytokines in spleen. Results : Total number of cells in the spleen and FACS analysis of T lymphocytes activated in the spleen showed that the SWCC combined treated group had much higher frequency of active cells than both single groups. The ratio of CD4+CD8+, CD4+CD69+ and CD4+CD25+ T cells in spleen, SWCC is higher than other groups except Nor in CD4+, CD4+CD69+, CD4+CD25+ T cells. The results of this study suggest that SWCC can help immune function via IL-2, IL-10, IL-12, IFN-${\gamma}$ cytokine production, increased T lymphocytes and splenocyte proliferation. Conclusion : Therefore, these results suggested that the SWCC combined extracts administration increase stronger immunity enhancement than when SW and CC adminstration.