The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory effect of Seungmagalgeun-tang extract in human mast cells

Human mast cell에서 승마갈근탕(升麻葛根湯)의 항염증 효과에 대한 연구

  • Keum, Joon-Ho (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Seo, Yun-Soo (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kang, Ok-Hwa (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Choi, Jang-Gi (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University) ;
  • Kwon, Dong-Yeul (Dept. of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, and Institute of Biotechnology, Wonkwang University)
  • 금준호 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 서윤수 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 강옥화 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 최장기 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소) ;
  • 권동렬 (원광대학교 약학대학 한약학과, 원광한약연구소, 원광생명공학연구소)
  • Received : 2013.08.15
  • Accepted : 2013.09.22
  • Published : 2013.09.30
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Abstract

Objectives : Seungmagalgeun-tang (SMGGT) is traditional medicine widely used for inflammatory disease and flu. But SMGGT exhibits potent anti-inflammatory activity with an unknown mechanism. To elucidate the molecular mechanisms of SMGGT water extract on pharmacological and biochemical actions in inflammation, we examined the effect of SMGGT on pro-inflammatory mediators in Phorbol-12-myristate-13-acetate (PMA)+A23187-stimulated mast cells. Methods : In the present study, pro-inflammatory cytokine production was determined by performing enzyme-linked immunosorbent assay (ELISA), reverse transcription polymerase chain reaction (RT-PCR), and western blot analysis to measure the activation of MAPKs. Cells were treated with SMGGT 1 h prior to the addition of 50 nM of PMA and $1{\mu}M$ of A23187. Cell viability was measured by MTS assay. The investigation focused on whether SMGGT inhibited the expressions of interleukin-6 (IL-6), interleukin-8 (IL-8) and mitogen-activated protein kinases (MAPKs) in PMA+A23187-stimulated mast cells. Results : SMGGT has no cytotoxicity at examined concentration (100, 250, and $500{\mu}g/ml$). Also, gene expression of IL-6 and IL-8 in HMC-1 cells stimulated by PMA+A23187 was down regulated by SMGGT. Furthermore, SMGGT suppressed the PMA+A23187-induced phosphorylation of extracellular signal-regulated kinase (ERK) and c-jun N-terminal Kinase(JNK). But, SMGGT could not regulate phosphorylation of p38 MAPK. Conclusions : These results suggest that SMGGT has inhibitory effects on PMA+A23187-induced IL-6 and IL-8 production. These inhibitory effects occur through blockades on the phosphorylation of ERK and JNK.

Keywords

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