• 디자인학연구
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• 제16권1호
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• pp.83-92
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• 2003

최근 3D 애니메이션은 급속한 하드웨어와 소프트웨어의 기술적 발전에 힘입어 실사에 가까운 애니메이션 연출이 가능하게 되어 영화, 광고, 게임, 디지털 영상물 등의 엔터테인먼트적인 요소로 많이 사용되고 있다. 3D 애니메이션 제작과정은 전통적인 애니메이션과는 달리 다양한 제작기술과 표현요소를 요하게 된다. 3D 애니메이션 제작에서 제작기술과 표현요소는 각각의 요소로 작용하는 것이 아니라, 서로 결합하여 제작하여야만 완성도가 높은 애니메이션을 얻을 수가 있다. 이에 본 고는 3D 애니메이션 Computer Process에 의한 작업 과정을 하드웨어와 소프트웨어를 바탕으로 한 기술적인 표현방식과 미학적, 조형적, 디자인적, 회화적 요소를 바탕으로 한 예술적 표현요소를 분석하고 기술적인 완성도와 표현력이 있는 애니메이션을 제작하는 방법을 모색해 본다.

• 한국엔터테인먼트산업학회논문지
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• 제13권8호
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• pp.255-266
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• 2019

본 연구는 캐나다 출신의 NFBC 소속 작가인 미쉘 꾸르놔이에가 사용하는 Ink on paper 기법을 고찰하고, 이를 통하여 실현된 애니메이션 "Soif"(2013)에 나타나는 표현양식인 메타모포시스(metamorphosis)가 내러티브의 구성을 위하여 구현된 의미와 상징을 고찰한다. 꾸르놔이에는 애니메이션의 사실적인 움직임을 벗어나기 위해 Ink on paper 표현양식을 사용하였으며, "Soif"에서는 극중 여자의 몸 캐릭터를 중심으로 일반적인 기승전결 형식이 아닌 인물과 사물의 이미지 변형을 메타모포시스 표현양식을 통해 보여준다. 이는 초현실주의와 무의식의 세계를 표현하기 위한 애니메이션에서의 즉흥적인 방법을 도입한 Ink on paper 기법을 통해 형상화된다. 그리고 이 작품에 적용된 자동기술법은 애니메이션을 추상적이고 형이상학적으로 표현하는데 영향을 주고 있다. 결국 이러한 표현양식과 기법은 작품에 등장하는 캐릭터의 연계성과 동일성을 강조하여 주제를 표현하는 데 매우 적절하게 작용한다.

• 한국멀티미디어학회논문지
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• 제12권8호
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• pp.1128-1141
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• 2009

게임 산업이 성장하면서 게임 그래픽 분야는 가장 빠르게 발전하고 있으며 지금은 실제 사진과 컴퓨터 그래픽을 구분하기 힘든 정도로 그래픽 기술은 양적으로나 질적으로 성장했다. 그러나 이러한 발전에도 불구하고 새롭게 출시되는 게임에 등장하는 캐릭터는 정서를 표현하지 않거나 소극적으로 표현하고 있다. 일반적으로 사람들은 얼굴의 표정이나 얼굴의 색(안색)에서 많은 정서 정보를 인지하는 것은 누구나 아는 사실이다. 그러므로 컴퓨터의 게임 환경에서 등장하는 캐릭터에게도 얼굴의 색의 변화를 통하여 유저에게 게임의 사실적인 느낌을 줄 수 있다. 본 논문에서는 기존의 맥박과 피부 온도만을 이용한 방법과는 다른 인간의 정서 이론을 기반한 정서 모형과 애니메이션의 정서표현 색 그리고 성격이론을 기반한 정서의 반응 속도를 결합하여 게임 캐릭터의 얼굴의 색을 동적으로 변화시켜 정서를 표현할 수 있는 얼굴 색 적응 기술 (Facial Color Adaptive Technique: FCAT)를 제안한다. 실제 얼굴 색 적응 기술을 이용하여 만든 얼굴색 모형(Facial Color Model : FCM)과 애니메이션 콘텐츠의 정서표현을 비교한 결과 정서 표현 색상과 변화율이 애니메이션과 유사하게 시뮬레이션 되었다. 또한 2차원 이미지뿐만 아니라 3차원 이미지에도 적용이 가능함을 확인 하였다.

• Communications for Statistical Applications and Methods
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• 제22권2호
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• pp.181-199
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• 2015

In a short history, RNA-seq data have established a revolutionary tool to directly decode various scenarios occurring on whole genome-wide expression profiles in regards with differential expression at gene, transcript, isoform, and exon specific quantification, genetic and genomic mutations, and etc. RNA-seq technique has been rapidly replacing arrays with seq-based platform experimental settings by revealing a couple of advantages such as identification of alternative splicing and allelic specific expression. The remarkable characteristics of high-throughput large-scale expression profile in RNA-seq are lied on expression levels of read counts, structure of correlated samples and genes, larger number of genes compared to sample size, different sampling rates, inevitable systematic RNA-seq biases, and etc. In this study, we will comprehensively review how robust Bayesian and non-parametric methods have a better performance than classical statistical approaches by explicitly incorporating such intrinsic RNA-seq specific features with flexible and more appropriate assumptions and distributions in practice.

• Plant Biotechnology Reports
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• 제5권2호
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• pp.157-167
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• 2011

In order to establish a reliable and highly efficient method for genetic transformation of pepper, a monitoring system featuring GFP (green fluorescent protein) as a report marker was applied to Agrobacteriummediated transformation. A callus-induced transformation (CIT) system was used to transform the GFP gene. GFP expression was observed in all tissues of $T_0$, $T_1$ and $T_2$ peppers, constituting the first instance in which the whole pepper plant has exhibited GFP fluorescence. A total of 38 T0 peppers were obtained from 4,200 explants. The transformation rate ranged from 0.47 to 1.83% depending on the genotype, which was higher than that obtained by CIT without the GFP monitoring system. This technique could enhance selection power by monitoring GFP expression at the early stage of callus in vitro. The detection of GFP expression in the callus led to successful identification of the shoot that contained the transgene. Thus, this technique saved lots of time and money for conducting the genetic transformation process of pepper. In addition, a co-transformation technique was applied to the target transgene, CaCS (encoding capsaicinoid synthetase of Capsicum) along with GFP. Paprika varieties were transformed by the CaCS::GFP construct, and GFP expression in callus tissues of paprika was monitored to select the right transformant.

• 한국의상디자인학회지
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• 제16권2호
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• pp.151-161
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• 2014

This study suggests method of expression of nail art utilizing Salvador Dali's Paranoiac Critical Method and produced the actual works based on discussions on Salvador Dali's painting, Paranoiac Critical Method, Nail Art's artistry and technique of expression. The result of the study is as follows: 1) Characteristic of Dali's painting is a Paranoiac Critical Method. If suggesting this characteristic in a method of expression of nail art, a good work which can be expressed on a small space, a nail, representing Dali is selected and 5 tips which are very similar to the rate of the painting are used in order to obtain an aesthetic effect just like a painting. And after composing on tips using the whole paining or part of painting, actual techniques of expression such as Hand Painting, Protranse or Water Decal are used. 2) the result of nail art produced utilizing Dali's Paranoiac Critical Method is as follows. Most of all, for nail art I with the topic of "The Persistence of Memory" (1931), after giving changes to 5 tips for the watch which is a part of the painting, Hand Painting technique is used using Acrylic Painting with colors which are similar colors to the painting. Then for nail art II which adopted "Slave Market with Invisible Bust of Voltaire" (1940), the whole painting is divided into 5 tips and Protranse technique which attaches printed paper onto nails was used. Lastly, for nail art III with the subject of "The Enigma of Desire My Mother, My Mother, My Mother" (1929), Water Decal which is emphasizing the parts of the painting and composing on 5 tips and copying printed picture onto Water Transfer Paper in order to attach on the nails is used. These nail arts show aesthetic characteristics such as fantasy, unconsciousness, grotesque, infinity, non-realism and horror just like paintings.

• Journal of Nutrition and Health
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• 제35권10호
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• pp.1053-1059
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• 2002

In post-genome period, the technique for identifying gene expression has been changed to high throughput screening. In the field of molecular nutrition, the need for this technique to clarify molecular function of the specific nutrient is essential. In this study, we have tested the zinc-regulated gene expression in zinc-deficient U937 cells, using cDNA microarray which is the cutting-edge technique to screen large numbers of gene expression simultaneously. The study result can be used for the preliminary gene screening data for clarifying, using monocyte U937 cell line, molecular Zn aspect in atherosclerosis. U937 cells were cultured in Zn-adequate (control, 12 $\mu$M Zn) or Zn-deficient (experimental, 0 $\mu$M Zn) ESMI media during 2 days, respectively. Cells were harvested and RNA was extracted. Total RNA was reverse-transcriptinized and synthesized cDNA probe labeled with Cy-3. fluorescent labeled cDNA probe was applied to microarray slide for hybridization slide, and after then, the slide was scanned using fluorescence scanner. ‘Highly expressed genes’ in Zn-deficient U937 cells, comparing to Zn-adequate group, are mainly about the genes for motility protein, immune system protein, oncogene and tumor suppressor and ‘Less highly expressed genes’ are about the genes for transcription, apoptosis associated protein, cell cycle, and several basic transcription factors. The results of this preliminary study imply the effectiveness of cDNA microarray for expression profiling of a singly nutrient deficiency, specially Zn. Furthur study, using tailored-cDNA array and capillary endothelial cell lines, would be beneficial to clarify molecular Zn function, more in detail.

• 대한가정학회지
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• 제35권1호
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• pp.31-46
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• 1997

This study aims to give a new modern light to a paper and search for a possibility of a paper as a new formative material. And then this study wants to suggest the formative effect of the paper operation as one technique which creates the idea of fashion design and to contribute in enlarging the possibility of expression of fashion design. The results of this study are as follow Firstly a paper is found to be expressed in new moulding forms by the various formative experiments because a paper can be easily and variously used as the material of expression. So the moulding images of paper operation are provided for the idea of fashion design and give much influences on the development of fashion design. Seccondly through the new light on a paper as the formative material it is demonstrated that a paper can be used for the development of many forms and the enlargement of the area of expression in the fashion design. Thirdly in the fashion design the formative effect of the paper operation not only give a variety to silhouette but also forms one technique of creation of new materials

• 한국군사과학기술학회지
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• 제14권4호
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• pp.710-718
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• 2011

Edema factor(EF) is a portion of anthrax toxin which produces edema when combined with protective antigen. This paper describes about technique for cloning, expression, purification and activity test of EF. Using the E. coli expression system, we could make recombinant EF protein although it's origin is Bacillus anthracis. And also we could culture massively and purify highly pure protein. Finally we confirm a enzyme activity of purified EF to increase intracellular cAMP level. Through establishing this technique, it can be possible to research about EF in depth and apply to expression and purification of many other protein in biology.

• 한국실내디자인학회:학술대회논문집
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• 한국실내디자인학회 2007년도 추계학술발표대회 논문집
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• pp.134-138
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• 2007

Presents age is diversified in all genres by fast development of information and digital, and expression of fixing is not. Architecture and interior space design is showing new paradigm through dematerializing, ex-formal, nonlinear. Glass material is expressing by various technique in space and outer skin to introduction of digital media and a high-tech technology. Expressive characteristics and Techniques of this glass material are showing form of homogenized life in this age, simulated life. Therefore, this study does theoretical investigation through dematerializing of glass material, and analyzes works after 2000. Wish to understand stream of indoor design of present age after analyzes expression special quality and technique of glass material that reflect age and make a study of symbolic expression characteristic.