• Journal of Fashion Business
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• v.14 no.5
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• pp.102-111
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• 2010

The pegging board planning for jacquard weaves was carried out to express a big enough design in 60 inch width. 20 Kinds of fabrics were fabricated by a jacquard loom with a warp density of 168 yarns/inch, a weft density of 100T, and a weight of 180-220g/$m^2$ or 250-300g/$m^2$. The result of sanitary test about Escherichia coli, Staphilococcus aureus, Klebsiella pneumoniae and Salmonella typhimurium showed a sterilizing power of over 90% after cultivating for 30, 60, 120 minutes. And also its property was sustained after laundrying 20 times. The flame retardant properties showed a good result and complied with the flame retardant criteria of KOFEI 1001. Abrasion, laundry and light fastnesses of flame retardant fabrics showed 4-5 grades. The fabrics manufactured from 30's fiber appeared a little fluff and pill on the fabric. Therefore, they need a shearing process after finishing all processes to decrease the fluff or pill on the fabric. The flame retardant fabric manufactured from 30's and 40's fiber showed increased strength and elongation after soaping and dyeing finishing because the fabrics were shrunk.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.11 no.2
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• pp.31-40
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• 2007

What is the "new paradigm"? It is impossible express it in one or two words, but if one had to; the closest might be the "holistic approach". The expression can be justified by the fact that the conclusions above lead to a greater intermixing of mathematics with engineering and natural sciences subjects, typically expressed in the form of examples of simplified real problems. They also lead to a greater intermixing of subjects within mathematics so that the courses should have less separation e.g. between symbolic and numerical mathematics. The conclusions also lead to the spreading the mathematics courses throughout all study years, not just the first two years. Of course, this should be done with great care in order to guarantee studies that are logically linked together. The new paradigm also means that the needs arising from industrial mathematics must be taken into account in the contents of engineering mathematics courses. Such topics are e.g. multivariate methods, statistics and use of mathematical software. What are we expected to gain from the paradigm shift? The primary benefit should be in obtaining more productive engineers equipped with a better degree of mathematical preparedness for engineering problems. But in addition, it should also promote more intensive use of applied mathematics and easier communication with professional mathematicians, often needed in complicated industrial problems.?Finally, it can be noted that the new paradigm is in harmony with the basic ideas of the CDIO (Conceive - Design - Implement - Operate) initiative for producing the next generation of engineers [1]. New ideas for engineering education can be found also in the homepage of SEFI (European Society for Engineering Education) [2].

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.11
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• pp.5605-5623
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• 2016

We propose a face recognition method that utilizes the LCP face descriptor. The proposed method applies a LoG mask to extract a face contour response, and employs the LCP algorithm to produce a binary pattern representation that ensures high recognition performance even under the changes in illumination, noise, and aging. The proposed LCP algorithm produces excellent noise reduction and efficiency in removing unnecessary information from the face by extracting a face contour response using the LoG mask, whose behavior is similar to the human eye. Majority of reported algorithms search for face contour response information. On the other hand, our proposed LCP algorithm produces results expressing major facial information by applying the threshold to the search area with only 8 bits. However, the LCP algorithm produces results that express major facial information with only 8-bits by applying a threshold value to the search area. Therefore, compared to previous approaches, the LCP algorithm maintains a consistent accuracy under varying circumstances, and produces a high face recognition rate with a relatively small feature vector. The test results indicate that the LCP algorithm produces a higher facial recognition rate than the rate of human visual's recognition capability, and outperforms the existing methods.

• Mycobiology
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• v.49 no.6
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• pp.599-603
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• 2021

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene HD1 (LeA1), we identified an endogenous LeU6 promoter in the L. edodes genome. We constructed a plasmid containing the LeU6 and glyceraldehyde-3-phosphate dehydrogenase (LeGPD) promoters to express the Cas9 protein. Among the eight gRNAs we tested, three successfully disrupted the LeA1 locus. Although the CRISPR-Cas9-induced alleles did not affect mating with compatible monokaryotic strains, disruption of the transcription levels of the downstream genes of LeHD1 and LeHD2 was detected. Based on this result, we present the first report of a simple and powerful genetic manipulation tool using the CRISPR/Cas9 toolbox for the scientifically and industrially important edible mushroom, L. edodes.

• Journal of Periodontal and Implant Science
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• v.41 no.3
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• pp.149-156
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• 2011

Purpose: Globular adiponectin (gAd) is a type of adipocytokine, which is mainly produced by adipose tissue. It has been reported that gAd acts as a pro- as well as an anti-inflammatory factor. Interleukin (IL)-6 and IL-8 are pro-inflammatory cytokines. To investigate the role of gAd on periodontal tissues, the expression of adiponectin receptor 1 (AdipoR1) and the effect of gAd on the expression of IL-6 and IL-8 were investigated in periodontal ligament (PDL) and gingival fibroblasts. Methods: PDL and gingival fibroblasts were cultured from human periodontal tissues. gAd derived from Escherichia coli and murine myeloma cells were used. The expression of AdipoR1 was estimated by reverse transcription-polymerase chain reaction and western blot The expression of cytokines was measured by enzyme-linked immunosorbent assay. Results: PDL and gingival fibroblasts expressed both mRNA and protein of AdipoR1. gAd derived from E. coli increased the production of IL-6 and IL-8, but polymyxin B, an inhibitor of lipopolysaccharide (LPS), inhibited IL-6 and IL-8 production induced by gAd in both types of cells. gAd derived from murine myeloma cells did not induce IL-6 and IL-8 production in those cells. gAd derived from E. coli contained higher levels of LPS than gAd derived from murine myeloma cells. LPS increased production of IL-6 and IL-8 in PDL and gingival fibroblasts, but pretreatment of cells with gAd derived from murine myeloma cells did not inhibit LPS-induced IL-6 and IL-8 expression. Conclusions: Our results suggest that PDL and gingival fibroblasts express AdipoR1 and that gAd does not act as a modulator of IL-6 and IL-8 expression in PDL and gingival fibroblasts.

• Korean Journal of Acupuncture
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• v.28 no.3
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• pp.63-71
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• 2011

Objectives : The aim of this study is to develop the system and evaluate the optical analytical technique that reflects acupoint pigmentation and extravasated blood by cupping. Methods : We designed the system able to express XYZ coordinate on local skin color. To evaluate measurement-accuracy, we compared with 24 Color Checker Chart by standardized Commission Internationale de l'Eclairage. After confirming the performance of system, we experimented with cupping which was 80 kPa negative pressure for 1 minute on left/right BL13. The X, Y and Z values were converted to R, G and B, $L^*$, $a^*$ and $b^*$, Erythema Index (E.I.), and Melanin Index (M.I). We compared and analyzed two cases on before/after cupping. Results : The R, G and B values which were converted by X, Y and Z values had high linearity as a high level of R-square (R: 0.969, G: 0.996, and B: 0.992). Moreover, we confirmed that it was possible to quantitatively analyzed the change in skin color by cupping using R, G, B, $L^*$, $a^*$, $b^*$, E.I., and M.I. Conclusions : Therefore, we proposed the new analytical technique for objectifying the oriental medical diagnostic method using cupping and optical sensing technique.

• International Journal of Industrial Entomology and Biomaterials
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• v.32 no.1
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• pp.1-11
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• 2016

Tropical tasar silkworm, Antheraea mylitta is a commercial forest silkworm in India that produces tasar silk, but never experimented in Uttarakhand, a Himalayan state of India. A. mylitta express divergent phenotypic characters under different ecological conditions; so, we studied the effect of seven forest tree species in two rearing seasons on variability in silk gland weight of Daba (bivoltine) ecorace of A. mylitta at Forest Research Institute in Dehra Dun, Uttarakhand during 2012 and 2013. We used two-way completely randomized block factorial design and Post HOC Tukey's HSD test to analyse the collected data and there after carried out multiple regression analysis. Results indicated that silk gland weight differed significantly between rearing seasons (DF=1, F=2333.98, p <0.05), host plants (DF 6, F= 1516.25, p <0.05) and their interactions (DF=6, F=7.10, p <0.05). Higher silk gland weight was found in second rearing season than the first on all the host tree species. Terminalia alata fed A. mylitta larvae showed the highest silk gland weight of 8.03 and 9.47 g in first and second rearing seasons, followed by T. tomentosa (7.19 & 9.01g), T. arjuna (6.8 & 8.08 g) and L. speciosa (6.57 & 7.83 g) fed larvae, respectively. Post HOC Tukey's HSD test indicated that silk gland weight of L. speciosa and T. arjuna fed larvae in both the rearing seasons did not differ significantly. E.I. analysis also confirmed that T. alata, T. tomentosa, T. arjuna and L. speciosa are better in their order of merit than T. bellirica , T. chebula and L. tomentosa. Multiple regression analysis indicates that larval weight gain is a strong predictor (β=1.002, t=346.777, p = <0.05) for the silk gland weight of A. mylitta ; however, larval duration had significant negative regression weight (β=-0.270, t=-8.436, p = <0.05) on mean weight of silk gland.

• KIEAE Journal
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• v.14 no.1
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• pp.121-129
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• 2014

As time passes, the aging of the plant building, the building's energy performance degradation than the initial plan does not express a situation could arise. This year, the certification of buildings certified in 2009 has expired for measures such as the situation required. In this study, management of national and international green building certification and re-certification was compared in two ways. First, the evaluation of green building certification system management assessments were compared. Second, the green building certification system for the re-certification analysis. As a result, G-SEED was not reflected life-cycle of building in management assessment and the commissioning of G-SEED is the UK and the U.S and other concepts of evaluation. In addition, the re-certification system is insufficient about substantial energy consumption of buildings. In this study, the revised the management assessments in conjunction with the re-certification system to manage the building is proposed to improve. In addition, the current evaluation of the existing building certification "existing building" and "building the first certified" as it is more efficient to separate the information into assessment was judged. Green building certification system to meet the purpose of management and operation, and disposal phases of the building to promote energy conservation and sustainability in order to the management a systematic and detailed evaluation and re-certification system developed for the revision of the specific items required and future research want to continue.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2004.11a
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• pp.138-149
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• 2004

In this study, we analyzed the gene expression data of Saccharomyces cerevisiae obtained from Holstege et al. 1998 to understand the relationship between expression level and nucleotide sequence of a gene. First, the correlation between gene expression and percent composition of each type of nucleotide was computed. It was observed that nucleotide 'G' and 'C' show positive correlation (r ${\geq}$ 0.15), 'A' shows negative correlation (r ${\approx}$ -0.21) and 'T' shows no correlation (r ${\approx}$ 0.00) with gene expression. It was also found that 'G+C' rich genes express more in comparison to 'A+T' rich genes. We observed the inverse correlation between composition of a nucleotide at genome level and level of gene expression. Then we computed the correlation between dinucleotides (e.g. AA, AT, GC) composition and gene expression and observed a wide variation in correlation (from r = -0.45 for AT to r = 0.35 for GT). The dinucleotides which contain 'T' have wide range of correlation with gene expression. For example, GT and CT have high positive correlation and AT have high negative correlation. We also computed the correlation between trinucleotides (or codon) composition and gene expression and again observed wide range of correlation (from r = -0.45 for ATA r = 0.45 for GGT). However, the major codons of a large number of amino acids show positive correlation with expression level, but there are a few amino acids whose major codons show negative correlation with expression level. These observations clearly indic ate the relationship between nucleotides composition and expression level. We also demonstrate that codon composition can be used to predict the expression of gene in a given condition. Software has been developed for calculating correlation between expression of gene and codon usage.

• BMB Reports
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• v.46 no.8
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• pp.404-409
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• 2013

Extracellular superoxide dismutase (EC-SOD) is a metallo-protein and functions as an antioxidant enzyme. In this study, we used lentiviral vectors to generate transgenic chickens that express the human EC-SOD gene. The recombinant lentiviruses were injected into the subgerminal cavity of freshly laid eggs. Subsequently, the embryos were incubated to hatch using phases II and III of the surrogate shell ex vivo culture system. Of 158 injected embryos, 16 chicks (G0) hatched and were screened for the hEC-SOD by PCR. Only 1 chick was identified as a transgenic bird containing the transgene in its germline. This founder (G0) bird was mated with wild-type hens to produce transgenic progeny, and 2 transgenic chicks (G1) were produced. In the generated transgenic hens (G2), the hEC-SOD protein was expressed in the egg white and showed antioxidant activity. These results highlight the potential of the chicken for production of biologically active proteins in egg white.