• Title/Summary/Keyword: Experiment study

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Change in plasma cortisol and glucose levels of Oncorhynchus keta according to water temperature

  • Young Seok Seo;Hyo Bin Lee;Joo Hak Jeong;Seong Jun Mun;Han Kyu Lim
    • Fisheries and Aquatic Sciences
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    • v.26 no.2
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    • pp.117-132
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    • 2023
  • Chum salmon (Oncorhynchus keta) is a species of anadromous salmonid inhabiting coastal rivers in the North Pacific and the Arctic in the Bering and is the most widely distributed among Pacific salmon species. It is an important fish species in Korea as the salmon releasing project is being actively carried out. This study was conducted to investigate changes in the physiological activity of O. keta according to water temperature. Three experiments were conducted according to the water temperature and period, and the plasma concentrations of cortisol and glucose were analyzed from the blood samples of the experimental groups. Experiment I is a short-term water temperature experiment, in which water temperature stimulation was given for 4 hours at water temperatures of 12℃, 16℃ (control), 20℃, and 24℃, and a recovery period was given for 4 hours. Experiment II is an experiment in which water temperature stimulation was given for 24 hours, 48 hours, and 72 hours at a high temperature of 24℃, and a recovery period was given for 12 hours, respectively. Experiment III is a long-term water temperature experiment, in which the water temperature was 12℃, 16℃ (control), 20℃, and 24℃ for 8 weeks. As a result of the experiment, in Experiment I, there was no significant difference in the survival rate between the experimental groups, but the concentration of cortisol and glucose in the plasma according to the set water temperature showed a significant difference. In Experiment II, there was no significant trend according to the maintenance time of the high-temperature state, but as the temperature increased, the plasma cortisol and glucose levels significantly increased compared to the control group. In Experiment III, all of the experimental group C (24℃) died in the 1st week, and there was no significant difference in the plasma glucose at the 1st and 8th weeks among the remaining experimental groups.

Analysis of the Difference in Pilot Error by Using the Signal Detection Theory (신호탐지론을 활용한 조종사 Error 차이 분석)

  • Kwon, Oh-Young
    • Journal of the Korean Society for Aviation and Aeronautics
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    • v.18 no.1
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    • pp.51-57
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    • 2010
  • This study was to analyze the difference in pilot error by using the Signal Detection Theory. The task was to detect the targeted aircraft(signal) which is different shape from many other aircraft(noise). From the two experiments, we differentiated the task difficulty followed by change in noise stimuli. Experiment 1 was to search the signal stimuli(fighter plane) while the noise stimuli(cargo plane) were increasing. The results from the Experiment 1 showed the tendency to decrease the hit rate by increasing the number of noise stimuli. However, the false alarm rate was not increased. The sensitivity(d') showed quite high. In Experiment 2, a disturbance stimulus(helicopter) was added to noise stimuli. The result was generally similar to those of Experiment 1. However, the hit rate was lower than that of Experiment 1.

Analysis of Representation Patterns Used by Elementary Teachers and Meaning-Making Processes in Electromagnetic Experiment Activities (전자기 관련 실험 활동에서 초등 교사가 사용한 표상 패턴과 의미 형성 과정 분석)

  • Chang, Jina
    • Journal of Korean Elementary Science Education
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    • v.39 no.2
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    • pp.204-218
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    • 2020
  • This study aims to investigate the representation patterns used by elementary teachers and their meaning-making process in electromagnetic experiments. In particular, we analyzed the representations depending on three levels of their abstractness: enactive representation (action based), iconic representation (image based) and symbolic representation (language based). For this, four experiment activities of two teachers were analyzed and the results are as follows. First, as an overall pattern of representation, an experiment subject is presented as the form of symbolic representation and the related concepts, experimental materials and methods are embodied through iconic representation. Then, through enactive representation, the actual experiments are implemented. The experimental results are primarily recorded through iconic representations and abstracted into symbolic representations to draw conclusions. The different levels of representations complement each other to expand their meanings, however, sometimes they also make inconsistent meanings among different levels. Based on these results, educational implications were discussed in terms of supporting and improving electromagnetic experiment activities.

Diagnostics on Gear Faults Using Transmission Error : Simulation vs Experiment (전달오차를 이용한 기어고장진단: 해석 vs 실험적 방법론)

  • Park, Jungho;Ha, Jongmoon;Choi, Jooho;Park, Sungho;Youn, Byeng D.
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2014.10a
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    • pp.499-502
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    • 2014
  • This paper presents a comparison study between simulation and experiment for fault diagnostics of a spur gear. In simulation, fault diagnostics using transmission error (TE) was performed and concluded to be valid. In a real experiment, however, it is not as easy to detect faults of gears using TE as in simulation. In this paper, after seeding the various faults like tooth crack of different length, tooth breakage and spalling in test rig, TE was calculated. Then, several signal processing techniques were performed to overcome the limitations of an experiment in detecting the fault signals of TE. After signal processing, we could detect the various faults of spur gears and different amplitude of TE sparks from cracks of different length. Then we discussed the difference between simulation and experment.

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A Study on the Prediction of Train Noise Propagation Using the Spark Discharge Sound Source (스파크음원을 이용한 철도소음 전파예측에 관한 기초적 연구)

  • Joo Jin-Soo;Kim Jae-Chul
    • Proceedings of the KSR Conference
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    • 2003.10c
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    • pp.132-137
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    • 2003
  • This paper concerns the prediction of railway noise propagation using scale model experiment in acoustics. In order to make acoustical experiment the digital signal processing technique are applied and spark discharge sound sources have been developed in which impulse response measured in 1/20 scale model railway. In the case of scale model experiment, it is difficult to realize sufficiently small size and directivity and to get sufficient sound energy and to get repeatability. Several type of Spark discharge sound source is made in laboratory. Experiment results are compared with the calculated results by the prediction model. As the results, it was found that railway noise could be predicted in acoustical scale model experiment using spark discharge sound source.

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The Elderly's Error Characteristics in Some Human Interactions (자극에 대한 상호작용 관점에서의 노인의 오류 특성)

  • Jung, Kwang-Tae
    • Journal of the Ergonomics Society of Korea
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    • v.30 no.1
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    • pp.109-115
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    • 2011
  • In this research, three topics were studied for the elderly. The first was the elderly's error characteristics for tracing task and the second was for depth perception. The characteristics were compared with the young's. Twenty two old people and twenty two young people participated in the tracing experiment, respectively. In the depth perception experiment, twenty three old people and twenty two young people participated. A depth perception apparatus and a tracing one were used in the experiment. In the depth perception error, a significant difference was not detected on gender. However significant differences were detected with age and viewing distance. In the tracing error, significant difference was detected with age, also. The third experiment was push-button operation and fifteen old people participated in the experiment. As the result, operation time error was increased with the button size and the moving distance. The result of this study will be able to be considered in its user interface design to reduce the elderly's error while using a product.

Design and Pilot Application of an Experiment Focusing on the Nature of Scientific Inquiry: Focus on the Epistemological Issues in the Process of Dry Ice Sublimation Experiment (과학 탐구의 본성에 초점을 둔 실험의 설계와 시범 적용 -드라이아이스 승화 실험에서 드러나는 인식론적 논제를 중심으로-)

  • Park, Jeongwoo;Lee, Sun-Kyung;Lee, Gyeong-Geon;Shim, Han Su;Shin, Myeong-Kyeong
    • Journal of The Korean Association For Science Education
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    • v.39 no.2
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    • pp.173-186
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    • 2019
  • The purpose of this study is to design and apply a pilot inquiry experiment focusing on the epistemological issues of scientific activities, and derive educational implications by analyzing experimental activities and reflective discussions. Three graduate students who major in science education participated in the study voluntarily. Participants showed the characteristics of stable enquiry in Experiment 1. However, the small but continuous changes in Experiment 2 led the experiment to a phase of fluid enquiry seeking new theories. Participants mobilized various resources, proposed new hypotheses, and models and requested additional experiments to verify them. In the process of reflective discussions, the participants led to the following three epistemological issues. First, at the beginning of the experiment, their observations were theoretically dependent. Second, when the observations were no longer coherent with theory, they face a crisis, and the adjustment of observation and theory proceeds. Third, stable enquiry and fluid enquiry are performed according to the relationship between observation and theory. The educational implications of school science inquiry based on the above process and results are as follows: First, this study shows that fluid enquiry can follow stable enquiry naturally, and examples of the activities are presented together. Second, in this study, it was confirmed that participants could draw up epistemological issues based on their experiences through reflective discussions following inquiry.

Assessment of Nutritional Components, Carotenoid Content and Physiological Activity of Maize Hybrid for Grain 'Kangilok' (강일옥 옥수수의 영양성분, 카로티노이드 함량분석 및 생리활성 평가)

  • Lee, Ki Yeon;Kim, Jai Eun;Hong, Soo Young;Kim, Tae Hee;Park, A-Reum;Noh, Hee Sun;Kim, Si Chang;Park, Jong Yeol;Ahn, Mun Seob;Kim, Hee Yeon
    • Journal of Food Hygiene and Safety
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    • v.32 no.6
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    • pp.513-520
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    • 2017
  • This study was performed to provide basic data of 'Kangilok'. The objective of this study was to investigate worth of 4 parts of maize hybrid for grain, 'Kangilok' for functional foods. The 4 parts are kernels, dehulled kernels, skin of kernels and cobs of 'Kangilok'. We evaluated moisture, crude ash, crude lipid, crude protein, crude fiber and mineral content of 'Kangilok'. The moisture of kernels, dehulled kernels, skin of kernels and cobs of 'Kangilok' were 11.27%, 12.40%, 9.45%, 8.85% and the crude ash were 1.26%, 0.73%, 3.19%, 1.42%. Each of the crude lipid were 3.84%, 2.69%, 8.59%, 0.46% and the crude protein were 9.40%, 9.96%, 12.10%, 2.80%. The crude fiber of kernels, dehulled kernels, skin of kernels and cobs of 'Kangilok' were 2.24%, 0.92%, 7.07%, and 33.51%. Among the mineral contents, Ca and K content of cobs were highest by 4.84 mg/100 g, 114.33 mg/100 g and Fe, Mn contents of skin of kernels were highest by 5.30 mg/100 g, 2.64 mg/100 g. Mg content of kernels was the highest by 27.42 mg/100 g. P content of kernels, dehulled kernels, skin of kernels and cobs were 1.20%, 0.96%, 2.41%, and 0.19%. It was performed test on anti-oxidative, anti-inflammatory activities of 60% ethanol extract from 4 parts of Kangilok. The anti-oxidative effect was measured by DPPH and ABTS radical scavenging activity. As a results, DPPH radical scavenging activity (10 mg/mL) was 72.59%~93.05% and ABTS radical scavenging activity (10 mg/mL) was 48.17%~79.88%. The anti-inflammatory effect was measured by ability to inhibit production nitric oxide (NO) in RAW264.7 cell. As a result, all the extract of 4 parts were showed significantly inhibitory effect on NO production. Carotenoid contents quantified by using HPLC. ${\beta}$-Carotene of carotenoid was not analyzed in all the sample. Lutein and zeaxantin ware analyzed in kernels and skin of kernels.

Inhibition of Pancreatic Lipase Activity and Adipocyte Differentiation in 3T3-L1 Cells Treated with Purple Corn Husk and Cob Extracts (자색옥수수 포엽과 속대 추출물의 리파아제 저해활성 및 3T3-L1 지방전구세포에서의 지방분화 억제효과)

  • Lee, Ki Yeon;Hong, Soo Young;Kim, Tae Hee;Kim, Jai Eun;Park, A-Reum;Noh, Hee Sun;Kim, Si Chang;Park, Jong Yeol;Ahn, Mun Seob;Jeong, Won Jin;Kim, Hee Yeon
    • Journal of Food Hygiene and Safety
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    • v.33 no.2
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    • pp.131-139
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    • 2018
  • Our review begins with the maize hybrid for grain, called 'Seakso 1,' which was developed in 2008 by the Gangwon Agricultural Research and Extension Services in Korea, and subsequently registered in 2011. In this study, we aimed to investigate the lipid metabolic enzyme activity and inhibitory effect on the adipocyte differentiation, in 3T3-L1 cells of the identified Seakso 1 corn husk and cob extracts (EHCS). We investigated the pancreatic lipase inhibitory effect and anti-adipogenic effect of EHCS.The lipid accumulation and adipocyte differentiation were measured by the procedure of Oil Red O staining, Real-time PCR and the Western blot analysis. The pancreatic lipase inhibitory activity of EHCS was measured at higher levels than those of the positive control (orlistat) at 100, 500, and $1,000{\mu}g/mL$. In particular, EHCS was noted as being significantly inhibited and including a measured adipocyte differentiation and lipid accumulation, when treated during the adipocyte differentiation process in 3T3-L1 cells. Based on the Oil Red O staining, EHCS inhibited lipid accumulation at 19.19%, 33.30% at $1000{\mu}g/mL$, $2000{\mu}g/mL$, respectively. The real-time PCR and Western blot analysis showed that EHCS significantly decreased in the mRNA expression and protein level of obesity-related factors, such as peroxisome-proliferatorsactivated-receptor-${\gamma}$ ($PPAR{\gamma}$) and CCAAT enhancer-binding-proteins ${\alpha}$ ($C/EBP{\alpha}$). This study potentially suggests that the Saekso 1 corn husk and cob extracts may improve lipid metabolism and reduce lipid accumulation.

A Study on Friction Characteristics of Motorcycle Disk Brake Using Design of Experiment (실험계획법을 이용한 이륜자동차 브레이크 디스크의 마찰특성에 관한 연구)

  • Jeun, Hwan-Young;Bae, Hwo-Jun;Park, Kyu-Jung;Ryu, Mi-Ra;Park, Heung-Sik
    • Tribology and Lubricants
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    • v.23 no.4
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    • pp.175-179
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    • 2007
  • The effect of manufacturing parameters on friction characteristics of motorcycle break system was studied using a design of experiment. Such parameters conditions have an effect on the frictional factor such as applied load, sliding speed, and number of ventilated disk hole. However, it is difficult to know the mutual relation of these factors. In this study, the friction characteristics using design of experiment containing 3 elements were investigated for an optimal condition for the best motorcycle break system employing full factorial design. From this study, the result was shown that the applied load in frictional factors was the most important, next to sliding speed, number of ventilated disk hole.