Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Assessment of Nutritional Components, Carotenoid Content and Physiological Activity of Maize Hybrid for Grain 'Kangilok'

강일옥 옥수수의 영양성분, 카로티노이드 함량분석 및 생리활성 평가

  • Lee, Ki Yeon (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Kim, Jai Eun (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Hong, Soo Young (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Kim, Tae Hee (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Park, A-Reum (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Noh, Hee Sun (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Kim, Si Chang (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Park, Jong Yeol (Hongcheon Maize Experiment, Gangwon-do Agricultural Research and Experiment Services) ;
  • Ahn, Mun Seob (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services) ;
  • Kim, Hee Yeon (Agriproduct Processing Experiment Station, Gangwon-do Agricultural Research and Experiment Services)
  • 이기연 (강원도농업기술원 농식품연구소) ;
  • 김재은 (강원도농업기술원 농식품연구소) ;
  • 홍수영 (강원도농업기술원 농식품연구소) ;
  • 김태희 (강원도농업기술원 농식품연구소) ;
  • 박아름 (강원도농업기술원 농식품연구소) ;
  • 노희선 (강원도농업기술원 농식품연구소) ;
  • 김시창 (강원도농업기술원 농식품연구소) ;
  • 박종열 (강원도농업기술원 옥수수연구소) ;
  • 안문섭 (강원도농업기술원 농식품연구소) ;
  • 김희연 (강원도농업기술원 농식품연구소)
  • Received : 2017.10.19
  • Accepted : 2017.10.30
  • Published : 2017.12.30
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Abstract

This study was performed to provide basic data of 'Kangilok'. The objective of this study was to investigate worth of 4 parts of maize hybrid for grain, 'Kangilok' for functional foods. The 4 parts are kernels, dehulled kernels, skin of kernels and cobs of 'Kangilok'. We evaluated moisture, crude ash, crude lipid, crude protein, crude fiber and mineral content of 'Kangilok'. The moisture of kernels, dehulled kernels, skin of kernels and cobs of 'Kangilok' were 11.27%, 12.40%, 9.45%, 8.85% and the crude ash were 1.26%, 0.73%, 3.19%, 1.42%. Each of the crude lipid were 3.84%, 2.69%, 8.59%, 0.46% and the crude protein were 9.40%, 9.96%, 12.10%, 2.80%. The crude fiber of kernels, dehulled kernels, skin of kernels and cobs of 'Kangilok' were 2.24%, 0.92%, 7.07%, and 33.51%. Among the mineral contents, Ca and K content of cobs were highest by 4.84 mg/100 g, 114.33 mg/100 g and Fe, Mn contents of skin of kernels were highest by 5.30 mg/100 g, 2.64 mg/100 g. Mg content of kernels was the highest by 27.42 mg/100 g. P content of kernels, dehulled kernels, skin of kernels and cobs were 1.20%, 0.96%, 2.41%, and 0.19%. It was performed test on anti-oxidative, anti-inflammatory activities of 60% ethanol extract from 4 parts of Kangilok. The anti-oxidative effect was measured by DPPH and ABTS radical scavenging activity. As a results, DPPH radical scavenging activity (10 mg/mL) was 72.59%~93.05% and ABTS radical scavenging activity (10 mg/mL) was 48.17%~79.88%. The anti-inflammatory effect was measured by ability to inhibit production nitric oxide (NO) in RAW264.7 cell. As a result, all the extract of 4 parts were showed significantly inhibitory effect on NO production. Carotenoid contents quantified by using HPLC. ${\beta}$-Carotene of carotenoid was not analyzed in all the sample. Lutein and zeaxantin ware analyzed in kernels and skin of kernels.

본 연구는 마치종 황색 알곡 품종으로 등록된 강일옥 옥수수 알곡의 처리별 시료 및 속대의 일반성분, 생리활성 검정, 색소 함량을 분석하여 기초자료로 제공하고자 수행되었다. 강일옥 옥수수를 알곡, 거피된 알곡, 알곡 껍질 및 속대로 분리하여 일반성분 분석 결과, 수분함량은 거피된 알곡이 12.04%로 가장 높았고 알곡 껍질의 조회분, 조지방, 조단백질 함량은 각각 3.19%, 8.59%, 12.01%로 강일옥 옥수수 처리별 시료 중 가장 높은 함량을 나타내었다. 강일옥 옥수수 처리별 시료의 무기성분 함량을 분석한 결과, 칼슘, 칼륨, 마그네슘, 철, 망간 및 인의 6종이 검출되었으며 공통적으로 검출된 주요 무기성분은 칼륨과 인이었다. 강일옥 옥수수 속대의 칼륨 함량이 114.33 mg/100 g으로 가장 높았으며 알곡 껍질의 인 함량이 2412.40 mg/100 g으로 가장 높게 측정되었다. 강일옥 옥수수 처리별 시료의 항산화 활성 검정 결과, 강일옥 옥수수 속대 추출물의 DPPH radical 소거능은 93.05%, ABTS radical 소거능은 79.88%로 처리별 시료 중 가장 높게 측정되었으며 알곡, 거피된 알곡 및 알곡껍질 추출물의 DPPH 소거능은 모두 70% 이상으로 우수한 항산화활성을 나타내었다. 강일옥 옥수수 처리별 시료의 항염 활성 검정 결과, 강일옥 옥수수 처리별 시료 추출물은 Raw264.7 대식세포의 생존율에 영향을 미치지 않은 것으로 나타났으며 NO 생성량은 모든 시료 처리군에서 LPS 단독 처리군(con(+))에 비하여 유의적으로 NO 생성을 억제 하는 것으로 나타났다. 강일옥 옥수수 처리별 건조분말시료의 카로티노이드 색소 함량을 분석한 결과, 모든 시료에서 ${\beta}$-carotene은 검출되지 않았고, lutein과 zeaxantin은 알곡과 알곡 껍질에서 검출되었으며 거피된 알곡과 속대에서는 검출되지 않았다. 강일옥 옥수수 알곡의 lutein과 zeaxantin의 함량은 각각 $115.30{\mu}g$/100 g, $50.92{\mu}g$/100 g이었으며 알곡껍질의 lutein과 zeaxantin의 함량은 각각 $37.98{\mu}g$/100 g, $19.27{\mu}g$/100 g이었다.

Keywords

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