• 제목/요약/키워드: Ex-situ test

검색결과 18건 처리시간 0.009초

Ex situ Coloration of Laccase-Entrapped Bacterial Cellulose with Natural Phenolic Dyes

  • Kim, Hyunjin;Song, Ji Eun;Kim, Hye Rim
    • 한국의류학회지
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    • 제45권5호
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    • pp.866-880
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    • 2021
  • This study aimed to ex situ colorize laccase-entrapped bacterial cellulose (BC) with natural phenolic dyes, namely,madder, turmeric, and cochineal, and to determine the effect of laccase entrapment on the dyeability of BC using color strength (K/S) analysis. Results showed that laccase entrapment improved the dyeability of BC and that pre-entrapment was the most effective method, compared with meta-entrapment and post-entrapment methods. In addition, surface characterizations confirmed the successful entrapment of laccase inside the BC nanostructure and retention of the cellulosic and crystalline structures of BC. The washing durability test confirmed that the K/S value of BC had improved after laccase entrapment. Furthermore, laccase-entrapped BC colorized with cochineal dye had the highest washing durability due to the high molecular weight of cochineal dyerelative to the other dyes. This study suggests a novel method for enhancing the dyeability and washing durability of BC colorized ex situ with natural phenolic dyes by laccase entrapment.

Prussian blue immobilization on various filter materials through Layer-by-Layer Assembly for effective cesium adsorption

  • Wi, Hyobin;Kim, Hyowon;Kang, Sung-Won;Hwang, Yuhoon
    • Membrane and Water Treatment
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    • 제10권3호
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    • pp.245-250
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    • 2019
  • Prussian blue (PB) is well known for its excellent $Cs^+$ ions adsorption capacity. Due to the high dispersibility of PB in aqueous phase, composite materials imbedding PB in supporting materials have been introduced as a solution. However, building PB particles inside porous supporting materials is still difficult, as PB particles are not fully formed and elute out to water. In this study, we suggest layer-by-layer (LBL) assembly to provide better immobilization of PB on supporting materials of poly vinyl alcohol sponge (PVA) and cellulose filter (CF). Three different PB attachment methods, ex-situ/in-situ/LBL assembly, were evaluated using PB leaching test as well as $Cs^+$ adsorption test. Changes of surface functionality and morphology during PB composite preparation protocols were monitored through Fourier transform infrared spectroscopy and scanning electron microscopy. The results indicate that LBL assembly led to better PB attachment on supporting materials, bringing less eluting PB particles in aqueous phase compared to other synthesis methodologies, such as ex-situ and in-situ synthesis. By enhancing the stability of the adsorbent, adsorption capacity of PVA-PB with LBL improved nine times and that of CF-PB improved over 20 times. Therefore, the results suggest that LBL assembly offers a better orientation for growing PB particles on porous supporting materials.

유류오염토양 복원을 위한 설계인자 선정에 관한 연구

  • 조장환;전권호;서창일;박정구
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2004년도 총회 및 춘계학술발표회
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    • pp.293-296
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    • 2004
  • The objective of this study was to decide the designing factor for remediaton of the contaminated site. The soil and ground-water samples were analyzed and hydro- geological characteristics was assayed for the survey of pollution level. Also air-permeability test and MPN(most probable number) test were conducted for selecting the designing factor. The contaminants were mainly found in north-west part of the site and were expected to move toward the south. Ex-situ technology was expected more useful than in-situ one with the results of air-permeability test saying that air permeability was relatively low. Additional microbes were expected for remediation efficiency because residual microbes were loosely populated. The choosing of the designing factor was requisite for remediation of contaminated site.

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Ex-situ 스퍼터링법에 의한 $V_2O_5$ 전 고상 박막전지의 전기화학적 특성 (Electrochemical Characteristics of $V_2O_5$ based All Solid State Thin Film Microbattery by Ex-situ Sputtering Method)

  • 임영창;남상철;전은정;윤영수;조원일;조병원;전해수;윤경석
    • 전기화학회지
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    • 제3권1호
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    • pp.44-48
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    • 2000
  • 상온에서 DC-magnetron sputtering으로 증착한 비정질의 $V_2O_5$ 박막을 양극물질로 하여 $V_2O_5/LIPON/Li$으로 구성된 박막형 리튬이차전지를 제작하였다. $V_2O_5$의 양극특성은 액체전해질을 이용한 half cell 구조에서 평가하였으며, $Ar/O_2$ 분압비의 변화에 따라 제작된 $V_2O_5$ 양극은 분압비 80/20에서 가장 좋은 특성을 보였다. 자체 제작한 $Li_3PO_4$ 타겟을 사용하여 RF-sputtering으로 순수한 질소 분위기 하에서 양극 위에 고체전해질 LIPON 박막을 형성하였으며, 1.2-4.0V vs. Li 구간에서 리튬에 대해 반응성이 없는 안정한 화합물임을 확인하였다. 음극으로 쓰인 약 $2{\mu}m$두께의 금속리튬박막은 진공 열 증착법으로 제조하였으며, $V_2O_5/LIPON/Li$의 박막형 리튬이차전지는 $1.2\~3.5V$ 구간에서 초기에 약 $150{\mu}A/cm^2{\mu}m$의 높은 방전용량을 나타내었다.

Isolation of an Indigenous Imidacloprid-Degrading Bacterium and Imidacloprid Bioremediation Under Simulated In Situ and Ex Situ Conditions

  • Hu, Guiping;Zhao, Yan;Liu, Bo;Song, Fengqing;You, Minsheng
    • Journal of Microbiology and Biotechnology
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    • 제23권11호
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    • pp.1617-1626
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    • 2013
  • The Bacterial community structure and its complexity of the enrichment culture during the isolation and screening of imidacloprid-degrading strain were studied using denaturating gradient gel electrophoresis analysis. The dominant bacteria in the original tea rhizosphere soil were uncultured bacteria, Rhizobium sp., Sinorhizobium, Ochrobactrum sp., Alcaligenes, Bacillus sp., Bacterium, Klebsiella sp., and Ensifer adhaerens. The bacterial community structure was altered extensively and its complexity reduced during the enrichment process, and four culturable bacteria, Ochrobactrum sp., Rhizobium sp., Geobacillus stearothermophilus, and Alcaligenes faecalis, remained in the final enrichment. Only one indigenous strain, BCL-1, with imidacloprid-degrading potential, was isolated from the sixth enrichment culture. This isolate was a gram-negative rod-shaped bacterium and identified as the genus Ochrobactrum based on its morphological, physiological, and biochemical properties and its 16S rRNA gene sequence. The degradation test showed that approximately 67.67% of the imidacloprid (50 mg/l) was degraded within 48 h by strain BCL-1. The optimum conditions for degradation were a pH of 8 and $30^{\circ}C$. The simulation of imidacloprid bioremediation by strain BCL-1 in soil demonstrated that the best performance in situ (tea soil) resulted in the degradation of 92.44% of the imidacloprid (100 mg/g) within 20 days, which was better than those observed in the ex situ simulations that were 64.66% (cabbage soil), 41.15% (potato soil), and 54.15% (tomato soil).

생물종(生物種) 다양성(多樣性) 및 삼림유전자원(森林遺傳資源) 보존(保存) 전략(戰略) (Strategy for Bio-Diversity and Genetic Conservation of Forest Resources in Korea)

  • 박용구
    • 한국산림과학회지
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    • 제83권2호
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    • pp.191-204
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    • 1994
  • 삼림의 급격한 황폐화의 원인은 지구 환경의 악화와 무분별한 목재 자원의 남벌에 있다. 이러한 대규모의 삼림자원의 파괴에 의해 사라져가는 삼림 면적의 크기도 중요하지만 그에 못지 않게 그 안에 들어있는 식물종이 감소되어 가고 멸종되어 가는 것이 더욱 큰 문제가 된다. 이러한 종의 감소나 멸종이 가시적인 것이라고 한다면 종내의 유전변이의 감소는 눈에 보이지는 않지만 진화과정에 있어서 종을 유지하는데 필수적인 유전자 변이 폭이 좁아지기 때문에 매우 심각한 문제가 된다. 재배작물에 있어서 유전자 보존은 육종을 위한 측면에서 중요한 연구분야로 인식되어 왔다. 그러나 야생종인 삼림의 경우에는 현재 인간이 육종에 필요한 최소한의 개체만 유지 보존함으로써 유전변이가 심하게 축소되어 지속적으로 생존 진화할 수 있는 기본적 유전자 변이를 잃어버리게 될 위험에 처할 수 있기 때문에 삼림의 유전자보존이 절대적으로 필요한 것이다. 현재의 삼림 유전자 보존 정책은 현지보존, 현지외 보존, 시설내 보존으로 나누어 수행하고 있는데 아직도 그 방법이 확정되어 있지 않아서 많은 시행착오를 거듭하고 있다. 특히 광범위하게 분포되어 있는 같은 종의 삼림내 임목들간의 유전자변이를 조사 분석할 적당한 방법이 없으며 (동위효소변이에 많은 것을 의존하고 있으나 동위효소변이만으로 충분하지 못하다), 현지보존의 경우에도 얼마나 큰 집단을 또 어떤 행태로 보존해야 하는가에 대한 집단유전학적 이론 정립이 완전하지 못하다. 또한 현지외 보존의 경우 현지보존림의 유전변이를 빠짐없이 포함되도록 조성해야 할 구체적인 방법을 알지 못하고 있는 실정에 있다. 시설내 보존의 경우 종자 보관이나 화분 보관과 같은 기술적인 것은 재배작물의 방법을 적용하면 되지만 어떤 집단의 종자나 화분을 채집 보관해야 하는지에 대한 집단유전학적 근거가 아직 확실히 마련되고 있지 않다. 시설내 보존인 경우 기왕에 육종에 의해 선발된 개체를 유전자형(개체) 상태로 보존함으로써 부가가치를 높일 수 있을 것이며, 이러한 연구는 새롭게 개발되고 있는 조직배양 및 유전공학적 기법을 이용하므로써 발전할 수 있는 여지가 많은 연구 분야이다. 현지보존의 경우 유전자 보존만의 목적으로 조성된 삼림뿐만 아니라 다른 목적으로 보호 받고 있는 많은 삼림, 예를 들면 국립, 도립공원, 보안림, 노거수 등에 대한 적절한 생태유전학적인 연구를 통하여 유전자원으로 이용할 수 있는 방법이 강구되어야 하며, 현지외 보존의 경우에도 유전자원 보존림의 조성 뿐만 아니라 임목육종과정에서 기 조성되어 있는 채종원, 산지시험림, 차대검정림, 클론보존원 등에 대해서도 적절한 유전학적 연구 조사를 수행함으로써 현지외 유전자 보존림으로 이용할 수 있게 될 것이다.

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모형빙 생성 시 승온 시간에 따른 모형빙의 강도 특성 연구 (A Study on the Strength Characteristics of Model Ice for Warm-up Time during Model Ice Preparation)

  • 정성엽;하정석
    • 대한조선학회논문집
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    • 제57권1호
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    • pp.15-22
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    • 2020
  • Understanding the strength characteristics of model ice is an important issue for model testing in an ice model basin to estimate the ship performance in ice. In particular, the mechanical properties of the model ice including elastic modulus, flexural strength and compressive strength are key consideration factors. In order to understand the characteristics of the model ice during warm-up phase at KRISO's ice model basin, the strength properties are tested in this study. The infinite plate-bending method, in-situ cantilever beam test and ex-situ uniaxial compressive test are conducted to determine the strength properties of model ice. The strength characteristics of the model ice are then analyzed in terms of the warm-up phase and seasonality. These results could be valuable to quality control of the model ice characteristics in KRISO's ice model basin and to better understand the variations in strength properties during the ice model tests.

Impact of gamma radiation on 8051 microcontroller performance

  • Charu Sharma;Puspalata Rajesh;R.P. Behera;S. Amirthapandian
    • Nuclear Engineering and Technology
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    • 제54권12호
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    • pp.4422-4430
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    • 2022
  • Studying the effects of gamma radiation on the instrumentation and control (I&C) system of a nuclear power plant is critical to the successful and reliable operation of the plant. In the accidental scenario, the adverse environment of ionizing radiation affects the performance of the I&C system and it leads to inaccurate and incomprehensible results. This paper reports the effects of gamma radiation on the AT89C51RD2, a commercial-off-the-shelf 8-bit high-performance flash microcontroller. The microcontroller, selected for the device under test for this study is used in the remote terminal unit for a nuclear power plant. The custom circuits were made to test the microcontroller under different gamma doses using a 60Co gamma source in both ex-situ and in-situ modes. The device was exposed to a maximum dose of 1.5 kGy. Under this hostile environment, the performance of the microcontroller was studied in terms of device current and voltage changes. It was observed that the microcontroller device can operate up to a total absorbed dose of approximately 0.6 kGy without any failure or degradation in its performance.

토양세척을 위한 계면활성제의 효과적 선정 (Efficient Screening of Surfactant for Soil Washing)

  • 신현무;이상화
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 1999년도 추계학술발표회
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    • pp.7-11
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    • 1999
  • Soil washing process being operated in ex-situ mode using surfactants could be appropriate one of the most effective one for remediation. The choice of surfactants has been considered most significantly to accomplish tile reduction of expenditure and the increase of efficiency. This study was carried out screening test and solubility, washing experiment, and surfactant sorption experiments for 18 kinds of surfactant obtained. Results from each surfactant's PSR obtained by the slope indicated that nonionic surfactants have much higher solubility for HOCs than anion surfactants for that. The washing experiment to find out a removal efficiency of each surfactant's TPH, LE1017 and LE1019 showed high removal efficiency. Through on the result of estimating the extent of adsorption of surfactants for soils, nonion surfactants showed higher adsorption to soils than anion surfactants.

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남성의 라이프 스타일과 매일의 의복선택동기에 관한 연구 (A Study on Life Style and Daily Clothing Selection Motives of Men)

  • 한화숙
    • 복식
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    • 제28권
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    • pp.137-150
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    • 1996
  • The objectives of this study were to classify daily clothing selection motives and to group men into life style types and to examine the differences in daily clothing selection motives according to the life style types. Questionnaire comprised of three sections: 36 Likert type items of life style measure; 29 Likert type items of daily clothing selection motives measure: and 4 demographic variables. Samples were 267 salary men in gheir 20's to 30's in Seoul Korea. The data were analyzed using factor analy-sis cluster analysis one-way ANOVA Dun-can's multiple range test and t-test. 1. Five factors of life style were derived by factor analysis ; F. 1 'appearance oriented' f. 2. 'positive activity' F. 3 'economy oriented': F.4 'active-leisure';F. 5 'contemporary' Four types of life style of men were defined by cluster analysis of the five factors: T. 1'posi-tive activity'; T. 2.'economy interest'; T. 3'contemporary' : T 2'economy interest' T.3'contemporary' ; T. 4 'appearance interest' 2. Four factors of daily clothing selection motives were derived by factor analysis: F.1 'clothing harmony'; F.2 'clothing utility'; F.3 'psychological influence'; F.4 'exogenous situ-ation adaptation 3. There were significant differences in daily clothing selection motives according to the life style types. The types of positive activity economy interest and contemporary had 'clothing utility' motive more than appearance interest type. Economy interest type had 'hpsychological influence' motive more than other three types. Economy interest type and appearance interest type had 'exogenous situ-ation adaptation' motive more than other two types 4. 20's and unmarried men had'psychological influence' motive more than 30's and married. Upper class had 'clothing harmony' and 'ex-ogeneous situation adaptation' motives more than middle and low. Men with less education had ' clothing utility' and 'exogenous situation adaptation' motives more than did more edu-cation.

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