• 한국미생물·생명공학회지
• /
• 제14권2호
• /
• pp.199-203
• /
• 1986

Wood chip을 support로 충전한 관형발효조에 Saccharomyces formosensis 효모용액을 통과시켜 고정화한 관형발효조에서 연속 발효실험을 수행한 결과 wood chip은 support로서 높은 효모 고정화율을 나타내었다. 관형발효조에서 발효실험시 유량 및 농도의 변화시 정상상태에 도달하는데 약 60시간 소요되었고, 정상상태에서 ethanol수율은 0.473-0.483로서 회분식 발효조 경우보다 높은 값을 나타냈다. 정상상태에서 ethanol생산성 (공간체적 기준)은 ethanol농도 61.4-65.7 g/l 범위에서 24.4-19.1g EtOH/$\ell$.hr를 나타내어 다른 고정화법에 의한 것과 비슷하게 높았다. 관형발효조에서 410시간 연속 발효운전시 오염현상이 없었고 효모생존율은 88-93%로 유지되었다.

• Environmental Analysis Health and Toxicology
• /
• 제4권3_4호
• /
• pp.15-28
• /
• 1989

The effects of ethanol on the toxicity of 2, 4-Dichlorophenoxyacetic acid in ICR mice were examined. The results were summerized as follows; 1. The LD$\sub$50/ of 2.4-dichlorphenoxyacetic acid sodium injected intraperitoneally in mice was approximately 367 mg/kg. When the animals were administered with ethanol, 2 ml/kg or 4 ml/kg the LD$\sub$50/ of 2.4-D sodium were decreased to 338 mg/kg or 32 mg/kg, respectively. 2. In the acute experimental group, spontaneous motor activity in the ANIMEX system exhibited dose-dependent decrease in mice administered with 2.4-D sodium and ethanol. 3. However, in subacute experimental group, spontaneous motor activity in the ANIMEX system exhibited dose-dependent increase in mice treated with ethanol.

• 한국미생물·생명공학회지
• /
• 제24권6호
• /
• pp.726-732
• /
• 1996

To improve the fermentation characteristics(such as starch-degradability, ethanol tolerance, sugar and high-temperature tolerance) of recombinant haploid yeast Saccharomyces diastaticus K114, hybridization technique was used. The hybridization partner was S. diastaticus 1177 which had good glucoamylase activity and fermentabi- lity. The best hybrid HH64 showed improved ethanol tolerance, sugar and high-temperature tolerance. Especia- lly, the starch-fermentability was significantly improved, since the hybrid produced 1.60% (w/v) ethanol from 4% (w/v) starch, while the recombinant haploid K114 produced 1.30% (w/v) ethanol. The optimum temperature and pH for the starch-fermentation by the hybrid HH64 was 30$\circ$C and 5, respectively. The hybrid yeast HH64 produced 7.5% (w/v) ethanol directly from 20% (w/v) starch.

• KSBB Journal
• /
• 제4권2호
• /
• pp.69-73
• /
• 1989

Xylosw의 초기 농도가 2%, 4%, 8% 그리고 10%의 경우에 대하여 회분식 발효 실험을 하였다. 최대 ethanol수율은 2%, 4%, 8%과 10%의 xylose의 농도에 대하여 각각 0.46, 0.45, 0.43 그리고 0.42로 나타났다. Xylitol은 넓은 범위의 specific oxygen supply rate와 xylose 농도에서 거의 생성되지 않았다. 최대 specific productivity는 2-10%의 농도에 대하여 0.11, 0.11, 0.241, 0.0961g ethanol/hr-g DCW의 값을 보여주었다.

• Journal of Life Science
• /
• 제9권2호
• /
• pp.15-18
• /
• 1999

Tempting to further understand the molecular mechanism of pharmacological action of ethanol, we investigated the acute effects of ethanol on the GABA-activated current (IGABA) of the cultured Sprague-Dawley rat hippocampal neurons in primary culture using the whole-cell patch-clamp technique. Patch-clamp recordings revealed that ethanol potentiated the Cl- current in a concentration-dependent manner(1-300mM) in the majority of the cell studied. This study demonstrates that ethanol can potentiate IGABA in mammalian central neurons.

• 한국분무공학회지
• /
• 제15권3호
• /
• pp.150-156
• /
• 2010

Ethanol has properties of a lower setting point, higher oxygen contents, lower cetane numbers, and also higher volatility compared to biodiesel. Thus, biodiesel fuel can be improved in the fluidity of blending fuel and exhaust emissions by blended ethanol fuel. This research aims to understand combustion characteristics of biodiesel-ethanol blending fuel inside a constant volume chamber. High speed camera was applied to visualize the physics of development of combustion processes, and combustion pressure and exhaust emissions were measured at several blending ratios of ethanol and biodiesel fuel. This information may contribute to improve the performance of biodiesel engine and reduce emissions in future.

• Journal of Ginseng Research
• /
• 제9권1호
• /
• pp.72-85
• /
• 1985

Attempts were made to see if feeding of ginseng ethanol extract could affect proper- ties of rat brain lactate dehydrogenase such as specific activity, heat stability, Km for substrate, inactivation by 3-bromopyruvate and trypsin, and immune response. The following results were obtained. Specific activity of LDH was observed to reach maximum in 5 month after birth and then decrease steadily. However, that of LDH from rat fed with ginseng ethanol extract was found in rat fed with ginseng ethanol extract. 3-bromopyruvate was shown to inactivate LDH-5 from old rat fed. Inactivation of LDH-5 by trypsin was remarkable in old rat fed. Km value for pyruvate in old rat fed was remarkably decreased. Cumulative results suggest that ginseng ethanol extract could affect conformational change of LDH responsible for altered properties through unknown mechanism.

• Journal of the Korean Wood Science and Technology
• /
• 제42권5호
• /
• pp.624-633
• /
• 2014

This study separated the crude extract (70% ethanol) of and its three fractions (hexane, chloroform and ethyl acetate extracts) on the basis of polarity indexes, and examined for their insecticidal activities against aphid (Uroleucon lactucicola). For crude extraction, the 70% ethanol extract showed the best extract yield (58.0%) and insecticidal activity (69.0%) among the various concentrations tested (water, 30% ethanol, 50% ethanol, 70% ethanol and 95% ethanol). The major chemical compounds of different fractions (hexane, chloroform and ethyl acetate extracts) were identified as eugenol by head space-GC-MS analysis. The hexane extract showed the highest eugenol content (43.7%) and insecticidal activity (80.0%). The insecticidal activity is accordingly believed to be attributable to the eugenol component. This may provide a useful starting point for the development of bio-pesticides.

• Journal of Electrochemical Science and Technology
• /
• 제3권1호
• /
• pp.50-56
• /
• 2012

Nickel modified NiOOH electrodes were used for the electrocatalytic oxidation of ethanol in alkaline solutions where the methods of cyclic voltammetry (CV) and chronoamperometry (CA) were employed. In CV studies, in the presence of ethanol, an increase in the current for the oxidation of nickel hydroxide is followed by a decrease in the corresponding cathodic current. This suggests that the oxidation of ethanol is being catalysed through mediated electron transfer across the nickel hydroxide layer comprising of nickel ions of various valence states. Under the CA regime the reaction followed a Cottrellian behavior and the diffusion coefficient of ethanol was found to be $1{\times}10^7cm^2s^{-1}$.

• 한국수소및신에너지학회논문집
• /
• 제28권6호
• /
• pp.697-703
• /
• 2017

This study investigated the high-efficient process for bioethanol from barley by various condition. First, higher concentrations of ethanol could be produced without loss of yield by using reducing water consumption. This is because it could prevent to increase viscosity despite reducing water consumption. Second, the ethanol yield could be improved by using reducing particle size of biomass (increase of enzyme reactive surface). Third, The addition of protease could have a considerable effect on yield of fermentation, which provides nutrients to the yeast. This results showed that bioethanol production would provide efficient ethanol production and lower production costs.