• 한국응용과학기술학회지
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• 제35권3호
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• pp.975-984
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• 2018

마치현 추출물에 대한 생리활성 소재로서 응용 가능성을 확인하고자 하였다. 마치현 추출물의 측정 결과, 플라보노이드, 폴리페놀 함량과 DPPH radical 소거능을 확인하였으며, 세포실험 결과 HaCaT, RAW 264.7, RBL-2H3 세포에서 유의한 세포독성은 나타나지 않았으며. $H_2O_2$에 의한 유발되는 산화적 스트레스에 대한 HaCaT 세포는 마치현 에탄올 $100{\mu}g/mL$ 농도에서 83% 보호효과가 확인되었다. RAW 264.7 세포에 대한 마치현 추출물의 항염 효과를 확인 결과 저농도에서도 nitric oxide 생성이 억제되었으며, S. aureus, S. epidermidis, P. acnes 균에서도 마치현 추출물의 농도 의존적으로 항균 활성이 확인되었다. 본 연구는 마치현 추출물의 항산화, 항염증 및 항균 효과를 가지는 생리 활성 물질로서 활용 가능성을 확인하였다.

• 대한본초학회지
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• 제35권1호
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• pp.35-44
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• 2020

Objectives : The objective of this study was to investigate the therapeutic effect of Corni Fructus 30% ethanol extract (CFE) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods : The subjects were divided into 4 groups ; Normal group (N, n=10), MIA-induced osteoarthritis control group (Con, n=10), indomethacin 5 mg/kg treated group (INDO, n=10), CFE 200 mg/kg treated group (CFE, n=10). Blood and articulation tissues were collected after two weeks of drug administration. Oxidative stress was analyzed with reactive oxygen species (ROS), peroxynitrite (ONOO-). And the Nuclear factor erythroid-2 (Nrf2), heme oxygenase-1 (HO-1), superoxide dismutase (SOD), catalase, glutathione peroxidase-1/2 (GPx-1/2), Nuclear Factor Kappa B p65 (NF-κBp65), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNFα), interleukin-6 (IL-6), Interleukin 1β (IL-1β), matrix metalloproteinase-1 (MMP-1), and tissue inhibitor of metalloproteinases-1 (TIMP-1) were investigated by western blot. Results : The administration of CFE showed a significant reduction of changes in relative hind paw weight distribution. Reactive oxygen species (ROS) and peroxy nitrite (ONOO-) levels of articulation tissues were significantly decreased in CFE compared to the control group. Western blot measurements of Nrf2, HO-1, SOD, catalase, GPx-1/2 showed that the CFE group was increased compared to the Con group. And western blot measurements of NF-κBp65, COX-2, iNOS, TNFα, IL-6, IL-1β showed that the CFE group was reduced compared to the Con group. Also CFE group decreased MMP-1 and increased TIMP-1. Conclusion : Based on the above results, it can be seen that osteoarthritis is improved when Corni Fructus 30% ethanol extract treated.

• 한국자기공명학회논문지
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• 제21권3호
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• pp.102-108
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• 2017

Escherichia coli responds to ever-changing external and internal stresses by rapidly adjusting its physiology for better survival. This adjustment occurs at all levels including metabolites as well as mRNAs and proteins. Although there has been many reports describing E. coli's adaptation to various stresses regarding transcriptomics or proteomics, only a few investigations have been reported regarding this adaptation viewed from metabolites' perspective. We applied four different types of stresses at four different doses as imposed by NaCl, sorbitol, ethanol, and pH to investigate the similarities or differences among the stresses, and which stress causes the largest perturbation of the metabolite composition. We profiled the metabolites under such external stresses by using two-dimensional NMR spectroscopy and identified 39 metabolites including amino acids, sugars, organic acids, and nucleic acids. According to our statistical analysis, the osmotic stress caused by sorbitol differentiated itself from others, while NaCl showed the largest dose dependent metabolic perturbations. We hope this work will form a foundation on which an approach to a successful protein production is systematically provided by a favorable metabolic environment by imposing proper external stresses.

• Natural Product Sciences
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• 제18권2호
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• pp.76-82
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• 2012

Stamens of Mesua ferrea L. are a well-known herbal drug used in Indian System of Traditional Medicine to treat various diseases. The claimed activity of this plant part is necessitated to investigate antioxidant and hepatoprotective activity. Authenticated plant sample was extracted with hexane, ethanol (EtOH) and water (aq.) using ASE 100 accelerated solvent extractor. Antioxidant activity was evaluated by means of different in vitro assays. Hepatoprotective effect was investigated on carbon tetrachloride induced oxidative stress in liver slice culture model. Cytotoxic marker lactate dehydrogenase (LDH) released in culture medium and the activity of lipid peroxidation along with antioxidant enzymes (AOEs) namely superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were estimated. Hexane and EtOH extracts were significantly inhibited DPPH, NO, SOD and $ABTS^+$ radical in dose dependent manner. The trade of phenol content was: aq. extract < hexane extract < EtOH extract. A significant correlation was shown by total phenol content and free radical scavenging activity of extracts. The culture system treated with hexane extract, EtOH extract or ascorbic acid exhibited significant depletion in LDH, lipid peroxidation, antioxidative enzymes SOD, CAT and GR. Hexane extract and EtOH extracts of stamen of M. ferrea protected liver slice culture cells by alleviating oxidative stress induced damage to liver cells.

• 한국축산시설환경학회지
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• 제20권4호
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• pp.147-154
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• 2014

Heat stress is a significant burden to animal production in most areas of the world. Improving our knowledge of physiological and metabolic mechanisms of acclimation may contribute to the development of procedures that may help to maintain health and production efficiency under hot temperature. The effect of Ulmus pumila (UP) extract in inducing Heat Shock Proteins (HSPs) expression in heat-stressed RAW264.7 macrophage cells was investigated. Cell viability assay showed a dose dependent increase in cells after treatment with UP for 24 hours. RT-PCR and western blot analysis showed that increasing concentrations of UP induce the expression of Heat Shock Factor 1 (HSF1) and dose dependently upregulated the expression of Heat shock protein 70 (Hsp70) and Hsp90. LPS-induced nitric oxide was dose-dependently reduced while phagocytic activity greatly recovered with UP treatment. These data demonstrated that UP can be a potential candidate in the development of cytoprotective agent against heat stress.

• 한국식품영양과학회지
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• 제32권2호
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• pp.278-286
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• 2003

본 연구는 에탄올을 급여하는 동안 일어나는 지질과산화에 의한 간 손상과 이에 대하여 $\beta$-carotene, retinyl acetate, 13-cis-retinoic acid의 효과를 비교ㆍ검토하고자 실시되었다. 혈 장내 MDA 함량은 에탄올 급여군 중 비타민 A를 결핍시킨 FE군과 $\beta$-carotene을 공급한 DE군에서는 감소되지 않았으나, retinyl acetate와 13-cis-retinoic acid 공급시킨 RE군과 RAE군에서 감소되었다. 간 미토콘드리아내 MDA함량은 에탄을 급여군 중 FE군과 $\beta$E군에서 높은 함량을 보인데 반해 RE군과 RAE군에서는 낮은 수준을 보였다. 에탄올 비급여군인 pair-fed군중에서는 비타민 A를 결핍시킨 FP 군에 비해 $\beta$-carotene과 retinyl acetate를 각각 공급한 5P와 RP군의 MDA 함량은 감소하였는데 반해 retinoic acid를 공급한 RAP군의 MPA 수준은 차이를 보이지 않았다. 혈장내 AP 활성도는 에탄올과 함께 $\beta$-carotene과 retinoic acid를 공급시킨 군에서 각각의 pair-fed군에 비하여 높은 활성도를 보였으며 간 마이크로솜내 G6P 활성도는 에탄올 급여군 중 비타민 A 결핍군만 pair-fed군에 비해 유의적으로 감소되었으며 비타민 A를 보충시켰을 때에는 활성도가 증가되었다. 간 사이토졸내 SOD 활성도는 에탄을 급여와 함께 비타민 A를 결핍시킨 군에 비해 13-ciz-retinoic acid을 공급시킨 군이 가장 높았으며 Pair-fed 군 중에서는 retiny acetate 공급군에서 가장 낮은 높은 활성도를 보였다. 간 사이토졸내 GSH-Px 활성도는 에탄올 급여군과 pair-fed 군 사이에 일정한 경향은 없었으며 간 사이토졸네 GST 활성도는 에탄올 급여군 중 $\beta$-carotene과 retinyl acetate 공급군이 각각의 pair-fed군에 비해 증가되었다. 간조직내 $\beta$-carotene함량은 $\beta$-carotene, 공급군에서만 검출되었고 에탄을 급여군이 pair-fed군에 비해 6배정도 높은 함량을 보였으며 혈장과 간조직 내 retinol 함량은 에탄올 급여로 낮은 함량을 보였으나 비타민 A형태 중 retinyl acetate 공급군이 가장 높았다. 간조직 내 retinoic acid 함량은 에탄을 급여군 중 $\beta$-carotene과 retinyl acetate 공급군이 각각의 Pair-fed 군에 비해 유의적인 감소를 보였다. 상기 연구 결과를 보면 에탄올 급여로 지질과 산화물 함량이 증가되고 관련 항산화 효소계의 활성도 변화로 보아 간 손상에 지질과산화 반응이 관여된 것으로 보여 지며 에탄올의 영향에 대한 비타민 A의 효과에는 retiny1 acetate가 항산화제로 작용하여 간조직의 급격한 손상을 효율적으로 경감시킬 수 있는 것으로 나타났다.

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 2005년도 제57차 추계 학술대회 연제집
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• pp.490-490
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• 2005

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2003년도 춘계학술대회
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• pp.76-76
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• 2003

• 한국작물학회:학술대회논문집
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• 한국작물학회 2003년도 추계 학술발표회 요지
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• pp.58-59
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• 2003

• 동의생리병리학회지
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• 제33권6호
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• pp.356-362
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• 2019

Scutellaria baicalensis (SB) has widely used in the treatment for various brain diseases in the field of Oriental medicine. Biofermantation of SB can make major chemical constituents of SB to pass blood-brain barrier easily and to have more potent anti-oxidant ability. There is a little information about the contribution of fermented SB (FSB) to the formation or maintenance of the neural plasticity in the hippocampus. The purpose of this study was to evaluate effects of FSB extract on hydrogen peroxide (H₂O₂) - induced impairments of the induction and maintenance of long-term potentiation (LTP), an electrophysiological marker for the neural plasticity in the hippocampus. From hippocampal slices of rats, the field excitatory postsynaptic potentials (fEPSPs) were evoked by the electrical stimulation to the Schaffer collaterals - commissural fibers in the CA1 areas and LTP by theta-burst stimulation by using 64 - channels in vitro multi-extracellular recording system. In order to induce oxidative stress to hippocampal slices two different concentrations (200, 400 μM) of H₂O₂ were given to the perfused aCSF before and after the LTP induction, respectively. The ethanol extract of FBS with concentration of 25 ㎍/ml, 50 ㎍/ml was diluted in perfused aCSF that had 200 μM H₂O₂, respectively. Oxidative stress by the treatment of H₂O₂ resulted in decrease of the induction rate of LTP in the CA1 area with a dose - dependent manner. However, the ethanol extract of FSB prevented the reduction of the induction rate of LTP caused by H₂O₂ - induced oxidative stress with a dose - dependent manner. These results may support a potential application of FSB to ameliorate impairments of hippocampal dependent neural plasticity or memory caused by oxidative stress.