• 한국동물위생학회지
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• 제39권3호
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• pp.175-181
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• 2016

Staphylococcus pseudintermedius is an important opportunistic pathogen of dog and cats. Since 2006 there has been a significant emergence of methicillin-resistant S. pseudintermedius (MRSP) mainly due to clonal spread. The aim of this study was to investigated the prevalence of antibiotic resistance and presence of mecA and femA gene in 91 S. pseudintermedius isolates isolated from dogs and cats associated with various clinic infections. Methicillin resistance was confirmed by oxacillin disc diffusion method. MRSP isolate was detected 19 isolates (20.9%). MRSP and methicillin-resistant S. pseudintermedius (MSSP) isolates were highly resistant to penicillin, kanamycin, tetracycline, erythromycin, trimethoprim-sulfamethoxazole, clindamycin, ciprofloxacin, enrofloxacin and choloramphenicol (100~47.3% and 90.3~33.3%, respectively). About 90% of MRSP isolates were multi-drug resistance (resistance to at least five or more antimicrobials), and MSSP isolates was ca 74%. Among the 91 isolates, mecA gene was detected in 25 isolates (27.5%, 19 in MRSP isolates and 6 in MSSP isolates), but none carried the femA gene. Our results indicated MRSA isolates show a strong resistance to antimicrobials commonly used in veterinary medicine. A continuous surveillance and monitoring should be called for to prevent the contamination and spread of MRSP in dogs and cats.

• Journal of Microbiology
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• 제44권3호
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• pp.336-343
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• 2006

We compared the antimicrobial resistance and clonal relationships among the community-acquired (CA) and hospital-acquired (HA) methicillin-resistant Staphylococcus aureus (MRSA) strains that were isolated from blood cultures in a university hospital over a 4-year period. A total of 131 MRSA isolates, including 28 CA-MRSA and 103 HA-MRSA strains, were identified; antimicrobial susceptibility testing indicated that the CA-MRSA isolates were more susceptible to erythromycin (21 % vs 6% ; P=0.02), clindamycin (46% vs 12%; P<0.01), ciprofloxacin (43% vs 11%; P<0.01), and gentamicin (43% vs 6%; P<0.01) than were the HA-MRSA isolates. Pulsed-field gel electrophoresis (PFGE) typing and antimicrobial resistance profiles separated the 20 CA-MRSA isolates into 14 and 10 different patterns, respectively, and the 53 HA-MRSA isolates were separated into 24 and 7 different patterns, respectively. Twenty-one (40%) of the 53 HA-MRSA isolates belonged to two predominant PFGE types, and most of them showed multi-drug resistant patterns. Four (20%) of the 20 CA-MRSA and 10 (19%) of the 53 HA-MRSA isolates fell into two common PFGE patterns, and each of them showed the same multi-drug resistant pattern. This study suggests that, although the CA-MRSA blood isolates showed diverse PFGE and antimicrobial resistance patterns, some of these isolates may have originated from the HA-MRSA strains.

• 한국미생물·생명공학회지
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• 제49권3호
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• pp.449-457
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• 2021

The aim of the present study was to survey the frequency of inducible and constitutive phenotypes and inducible cross-resistant genes by regulating the methylation of 23S rRNA (ermA, ermB, and ermC) and macrolide efflux-related msrA gene in Staphylococcus aureus and S. epidermidis strains. A total of 172 bacterial isolates (identified based on standard tests), were examined in this study. Antibiotic susceptibility was determined by the disk diffusion method, and all isolates were evaluated with respect to inducible and constitutive phenotypes. The presence of ermA, ermB, ermC, and msrA genes was investigated by a PCR assay. The constitutive resistance phenotypes showed a higher distribution among the isolates. R phenotype was detected more among S. epidermidis isolates (46.25%). ermB, ermC, and msrA genes were detected more in methicillin-resistant S. aureus (MRSA) and methicillin-resistant S. epidermidis (MRSE) isolates that had R and HD phenotypes (>77% strains). The ermA gene had the lowest frequency among MRSA, MRSE, MSSA, and MSSE strains (<14% isolates). Distribution of inducible resistance genes in MRSA and MRSE strains, and possibly other species, leads to increased constitutive resistance to erythromycin, clindamycin, and other similar antibiotics. Therefore, it can be challenging to treat infections caused by these resistant strains.

• 약학회지
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• 제30권2호
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• pp.55-61
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• 1986

A total of 130 of Staphylococcus strains isolated from various clinical specimens of admitted patients of a university hospital with systemic or severe cases of infection. All of these were tested for the antimicrobial susceptibility to 11 drugs of common use. The hospital strains isolated showed higher frequency of resistance against four drugs including gentamicin, penicillin, erythromycin, kanamycin but amikacin, cephalothin, streptomycin were effective. And also 47.7% of methicillin-resistant Staphylococcus were isolated from the clinical patients. However, isolated Escherichia coli strains showed higher frequency of resistance, but two drugs, tobramycin and gentamicin were effective to them.

• 산업식품공학
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• 제14권1호
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• pp.65-74
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• 2010

파김치에서 분리한 마늘 내성 유산균인 Lactobacillus plantarum TJ-LP-002 균주의 균체생육과 항균활성에 영향을 미치는 배양조건 및 배지조건을 조사하였다. 선정 유산균의 배양 상등액 내에는 acetic acid, citric acid, lactic acid, tartaric acid와 같은 유기산이 존재하였고, 배양 중에 lactic acid와 acetic acid의 생성이 크게 증가하는 것으로 확인되었다. 단백질분해효소를 비롯한 각종 효소 처리에 의해 항균활성이 소실되지 않아, 선정 유산균이 생산하는 항균활성은 단백질성 물질이 아닌 산 생성에 의한 작용일 것으로 판단되었다. 항생제와 생균제의 병용 가능성을 확인하기 위하여 선정 유산균의 항생제 감수성을 조사한 결과, neomycin sulfate, spectinomycin dihydrochloride, lincomycin hydrochloride에 내성을 나타내었고, streptomycin sulfate에는 감수성을 나타내었으며, ampicillin trihydrate, chloramphenicol, erythromycin, tetracycline hydrochloride, kanamycin sulfate에는 중간 내성을 나타내었다. L. plantarum TJ-LP-002는 배양온도 30${^{\circ}C}$, 초기 pH 7.0, 24시간의 배양조건에서 최적의 균체생육과 항균활성을 나타내었으며, 탄소원은 glucose 3%(w/v), 질소원은 yeast extract 3%(w/v) 첨가 시에 균체생육과 항균활성이 높게 나타났다. 무기염류는 manganese sulfate와 ammonium citrate가 항균활성에 많은 영향을 주는 것으로 나타났으며, 각 성분을 단독 첨가하는 것보다 혼합 첨가하는 것이 더 우수한 영향을 나타내는 것으로 확인되었다.

• 약학회지
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• 제48권6호
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• pp.364-368
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• 2004

The prevalence of resistance to a range of macrolides was determined for clinical isolates of common respiratory pathogens using NCCLS testing methods and interpretative criter ia.71.4% of Streptococcus pneumoniae, 62.3% of Staphylococcus aureus, 50.8% of coagulase-negative staphylococci and 4.4% of Strpetococcus pyogenes were erythromycin resistant. Also, the rates of resistance to other macrolides and clindamycin in these clinical isolates were as high as to eryth -romycin. Almost all of the macrolide-resistant isolates were positive for erm of the methylase gene, or mef of the efflux gene.

• 대한수의학회지
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• 제17권1호
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• pp.13-16
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• 1977

In order to know the current status of sensitivity of Corynebacterium renale to some antimicrobial agents of current use, the sensitivity of 37 strains isolated from urine and vaginal cavity of healthy Korean native cattle and dairy cattle was studied by agar plate dilution method, and their sensitivity according to serological types was compared. The antimicrobial agents tested were streptomycin (SM), erythromycin (EM), oxytetracycline (OT), penicillin G (PC), gentamycin (GM), kanamycin (KM), ampicillin (AP), leucomycin (LM), chloramphenicol (CM), colistin (CL), sulfadimethoxine (SDM) and sulfadiazine (SDZ). Most strains were highly sensitive to PC, GM, AP, LM and KM, and moderate to CM and OT, but resistant to SM, EM, CL, SDM and SDZ. Serological type II was more sensitive to EM, SDM and SDZ than type I and II, but more resistant to SM.

• Journal of Microbiology
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• 제45권1호
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• pp.64-68
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• 2007

In this study we examined the multi-drug resistance profiles of the tetracycline (TC) resistant genus Vibrio to determine its susceptibility to two ${\beta}-lactams$, ampicillin (ABPC), and mecillinam (MPC), as well as to macrolide, erythromycin (EM). The results showed various patterns of resistance among strains that were isolated from very close geographical areas during the same year, suggesting diverse patterns of drug resistance in environmental bacteria from this area. In addition, the cross-resistance patterns suggested that the resistance determinants among Vibrio spp. are acquired differently within the sediment and seawater environments.

• 한국동물위생학회지
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• 제25권3호
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• pp.259-273
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• 2002

The present study was conducted to investigate the prevalence, biochemical properties and serotypes of Salmonella organisms in the domestic animals in Western Gyeongbuk province during the period from January to December 2000. Salmonella spp were isolated from 51(1.62%) of 3,141 cases of domestic animals. Serotypes of isolates were S enteritidis 17(29.6% of isolates), S agona 11(20.3%), S rissen 8(14.8%), S gallinarum 4(7.4%), S derby 3(5.5%), S typhimurium 2(3.7%), S travis 1(1.8%), S montevideo 1(1.8%) and untypable 4(12.9%), in order. The majority of isolates were highly susceptible to amikacin, ampicillin, ciprofloxacin, cephalothin, chloramphenicol, trimethoprim-sulfamethoxazole, norfloxacin and gentamicin, whereas all isolates were resistant to bacitracin, erythromycin, tiamulin and tylosin, and the majority of them were highly resistant to penicillin, streptomycin and tetracycline.

• 한국미생물·생명공학회지
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• 제51권4호
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• pp.542-544
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• 2023

Methicillin-resistant Staphylococcus pseudintermedius Z0118SP0130 was isolated from eye specimen of a companion dog in South Korea. The complete genome of Z0118SP0130 consisted of a 2,663,277 bp chromosome and there was no plasmid. The strain was identified as the sequence type 45 and contained a mecA gene which comprised of staphylococcal cassette chromosome mec type Vb (5C2&5). Antimicrobial resistance to erythromycin, clindamycin, quinupristin-dalfopristin, trimethoprim-sulfamethoxazole, mupirocin, oxacillin, streptomycin, and gentamicin was observed in the strain.