• Biotechnology and Bioprocess Engineering:BBE
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• 제11권1호
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• pp.13-18
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• 2006

The antioxidant activity of the extract of Erigeron annuus was assessed by means of two different in vitro tests: bleaching of the stable 1,1-diphenyl-2-picrylhydrazyl radical (DPPH test) and the scavenging of authentic peroxynitrite in company with peroxynitrite generation from 3-morpholinosydnonimine (SIN-1). In both tests, the 85% aq. MeOH and n-BuOH soluble fractions of the crude extract showed a significant scavenging effect on peroxynitrite and DPPH radical in comparison to L-ascorbic acid. And bioassay-guided fractionation of the n-BuOH soluble fraction led to the isolation of three compounds: Apigenin (1), quercetin-3-O-glucoside (2), and caffeic acid (3). The structures of the isolated compounds were elucidated on the basis of their spectroscopic data and their antioxidant activities were measured by determining their capacity to scavenge peroxynitrite and the DPPH radical.

• KSBB Journal
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• 제19권1호
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• pp.57-61
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• 2004

본 연구에서는 항산화 활성을 갖는 유용한 선도 물질을 찾기 위한 일환으로 20종의 염생식물의 추출물에 대해 peroxynitrite와 DPPH 라디칼 소거 활성을 측정하였다. 그 결과 해당화 (Rosa rugosa), 개망초 (Erigeron annus), 냇씀바귀(Ixeris tamagawaensis), 띠 (Imperata cylindrica), 범행초(Tetragonia tetragonoides), 사철쑥 (Artemisia capillaris)의 MeOH 추출물에서 우수한 DPPH 라디칼 소거 효과가 확인되었다. 또한, 해당화(Rosa rugosa), 개망초 (Erigeron annus), 칠면초 (Suaeda japonica), 냇씀바귀 (Ixeris tamagawaensis), 띠 (Imperata cylindrica), 갯방풍 (Glehnia littoralis), 함초(Salicornia herbacea), 해국 (Aster spathulifolius), 범행초 (Tetragonia tetragonoides)의 MeOH 추출물과 CH$_2$Cl$_2$ 추출물에서 각각 peroxynitrite의 소거효과가 관찰되었다. 그 중에서도 해당화 MeOH 추출물의 DPPH 래디칼 소거효과는 100 $\mu\textrm{g}$/$m\ell$ (f.c.) 농도에서 87.51%로 나타났고, peroxynitrite 소거효과는 5 $\mu\textrm{g}$/$m\ell$ 농도에서 표품의 ONOO를 첨가했을 때 97.03%와 SIN-1을 첨가했을 때 98.48%로서 가장 강력한 항산화활성을 가지는 것으로 나타났다. 해당화의 peroxynitrite의 효과는 대조군으로 사용된 penicillamine보다도 우수하며, L-ascorbic acid와 비슷한 효과였다.

• Nutrition Research and Practice
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• 제8권4호
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• pp.352-359
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• 2014

BACKGROUND/OBJECTIVES: In this study, we determined the anti-inflammatory activities and the underlying molecular mechanisms of the methanol extract from Erigeron Canadensis L. (ECM) in LPS-stimulated RAW264.7 macrophage cells. MATERIALS/METHODS: The potential anti-inflammatory properties of ECM were investigated by using RAW264.7 macrophages. We used western blot assays and real time quantitative polymerase chain reaction to detect protein and mRNA expression, respectively. Luciferase assays were performed to determine the transactivity of transcription factors. RESULTS: ECM significantly inhibited inducible nitric oxide synthase (iNOS)-derived NO and cyclooxygenase-2 (COX-2) derived PGE2 production in LPS-stimulated RAW264.7 macrophages. These inhibitory effects of ECM were accompanied by decreases in LPS-induced nuclear translocations and transactivities of $NF{\kappa}B$. Moreover, phosphorylation of mitogen-activated protein kinase (MAPKs) including extracellular signal-related kinase (ERK1/2), p38, and c-jun N-terminal kinase (JNK) was significantly suppressed by ECM in LPS-stimulated RAW264.7 macrophages. Further studies demonstrated that ECM by itself induced heme oxygenase-1 (HO-1) protein expression at the protein levels in dose-dependent manner. However, zinc protoporphyrin (ZnPP), a selective HO-1 inhibitor, abolished the ECM-induced suppression of NO production. CONCLUSIONS: These results suggested that ECM-induced HO-1 expression was partly responsible for the resulting anti-inflammatory effects. These findings suggest that ECM exerts anti-inflammatory actions and help to elucidate the mechanisms underlying the potential therapeutic values of Erigeron Canadensis L.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2014년도 추계학술대회
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• pp.985-988
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• 2014

본 연구에서는 망초의 생리활성 효과를 탐색하기 위하여 에탄올 추출물에서의 항산화 활성을 측정하였다. 총 플라보노이드 함량은 잎 추출물이 42.19 mg/g으로 다른 부위 추출물보다 높았으며, 총 폴리페놀 함량은 꽃 추출물이 74.26 mg/g으로 가장 높은 것으로 나타났다. DPPH radical 소거활성은 1 mg/mL의 농도에서 잎, 꽃, 전초 그리고 뿌리 추출물에서 각각 91.75%, 86.95%, 83.95% 및 83.43%로 높은 활성을 나타내었으며 농도 의존적으로 활성이 증가하는 것으로 나타났다. SOD 유사활성은 1.0 mg/mL의 농도에서 부위별 추출물이 7.91~13.54%를 나타내었으며 꽃 추출물의 활성이 가장 높은 것으로 나타났다. 이상의 결과로 미루어 볼 때 망초의 부위별 에탄올 추출물이 우수한 생리활성 효과를 나타냄으로서 이를 이용한 기능성 식품소재로의 활용이 가능할 것으로 생각된다.

• 한국환경복원기술학회지
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• 제8권5호
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• pp.39-46
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• 2005

This study was conducted to investigate the heavy metal accumulation in wild plants. The samples from 5 plant species on 14 sites were taken on the roadside where urban and industrial regions in northern Gyeonggi do. Artemisia princeps var. orientalis, Chenopodium album L. var. centrobrum, Erigeron canadensis L., Rumex crispus L. and Taraxacum platycapum H. Dahlst were taken from different parts of the plants gathered in surveyed sites for analysing. This study classified which species were suitable to accumulators, excluders, or indicators which were provided as Phytoremediation for heavy metals(Cd, Cr, Ni, Pb and Zn) and considered the applicability of that work. 1. The mean accumulation of 6 heavy metals in plants was as follows; Zn> Cu> Cr> Pb> Ni> Cd in rows except for Ni and Pb in Erigeron. 2. The highest heavy metal accumulators were Taraxacum and Artemisia. 3. The comparison of heavy metal accumulations between the tops and roots in the plants was as follows; Zn> Cd> Pb> Ni> Cu> Cr. 4. Comparison of heavy metal accumulations between tops and roots, root parts were higher than the top parts in most of the plants. Especially, in Taraxacum, a ratio was over 1.0 in Cr, Ni, and Zn. Therefore, Taraxacum was an accumulator for 3 heavy metals. And Erigeron, Chenopodium, Rumex, Artemisia were good for excluders which had lower than 1.0.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.47-47
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• 2020

In this study, we aimed to study carnation italian ringspot virus (CIRV) in Erigeron annuus (L.) Pers. in Bonghwa County, Korea. The collected samples showed mosaic and malformation symptoms. To identify the virus species, we performed high-throughput sequencing, reverse transcription polymerase chain reaction, and cloning. The virus was confirmed to be an unreported species, and therefore we performed genome sequencing of the samples. The complete genome was 4,746 nucleotides in length. The CIRV contained five open reading frames (ORFs), and it showed the typical features of members of the genus Tombusvirus. Phylogenetic analyses revealed that ClRV isolates had the highest nucleotide identities with the CZ isolate (95.89%) from Korea. In recent years, these viruses have sporadically been reported in floral scent and medicinal plants. This research found the first natural host infected with CIRV, and provides baseline information to determine the correlation between weeds and crops.

• 한국식품영양과학회지
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• 제40권11호
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• pp.1507-1511
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• 2011

본 연구에서는 LPS에 의해 활성화된 RAW264.7 대식세포에서의 NO 생성 및 iNOS와 HO-1 단백질 발현의 변화를 측정하여 개망초(Erigeron annuus L.) 꽃(EAF) methanol 추출물의 항염증 효과를 확인하였다. RAW264.7 대식세포에 LPS를 처리한 결과 NO의 함량이 11.48 ${\mu}m$ 수준으로 증가하였다. 그러나 EAF methanol 추출물(25, 50, 100, 200 ${\mu}g$/mL)을 처리하였을 때 NO의 함량이 12.82, 9.61, 6.83, 2.52 ${\mu}m$로 농도 의존적으로 감소하였다. 또한 EAF methanol 추출물은 NO의 생성에 관여하는 iNOS 단백질의 발현을 농도 의존적으로 저해하였으며 HO-1 단백질의 발현을 유도하였다. EAF methanol 추출물에 의한 HO-1 발현이 NO 생성에 미치는 영향에 대해 확인하기 위해 HO-1의 inhibitor인 ZnPP를 사용하였다. 실험결과 ZnPP를 처리하여 HO-1의 발현을 저해하였을 때 추출물에 의해 감소된 NO의 함량이 다시 증가되었다. 본 연구 결과, EAF methanol 추출물은 염증을 유발하는 중요 인자인 NO를 저해하였고, iNOS의 발현을 억제시켰으며 산화적 손상으로부터 세포 보호 방어 기작에 관여하는 HO-1의 발현 등 항염증에 우수한 효과를 보였으며 항염증 연구의 기초 자료로 활용될 것으로 예상된다.

• Applied Biological Chemistry
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• 제51권1호
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• pp.55-59
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• 2008

국내산 망초(Erigeron canadensis L.) 의 지상부위를 수증기증류방식으로 처리하여 얻은 정유의 성분을 GC-MS로 분석하고 HaCaT 각질형성세포에 대한 세포독성을 조사하였다. 망초 정유에는 총 31종의 화학성분이 존재하는 것으로 분석되었는데, 이들을 성분별로 구분하면 탄화수소류 18종(전체 정유 중 91.99%), 에테르류 4종(0.49%), 알코올류 3종(3.59%), 아세테이트류 2종(2.92%), 알데히드류 1종(0.05%), 케톤류 3종(0.23%)이었다. 망초 정유에 함유되어 있는 주된 화합물은 탄화수소류인 D,L-limonene과 ${\delta}-3-carene$이었고, 이들의 함량은 각각 68.25%와 15.93%이었다. MTT assay를 통해서 망초 정유성분의 HaCaT 각질형성세포에 대한 세포독성을 검정한 결과, 정유의 농도와 세포독성간에는 상관관계가 있었으며$(y=17712x^2-2925.8x+118.55,\;R^2=0.9879)$, $IC_{50}$값은 0.027 ${\mu}g\;mg^{-1}$이었다.

• The Korean Journal of Ecology
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• 제18권2호
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• pp.275-283
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• 1995

Seral changes in floristic composition during abandoned field succession after shifting cultivation was investigated in eastern Kangwon-Do, Korea. According to the DCA ordination based on the floristic composition, sere of the dominant species was shown as follows: Digitaria sanguinalis, Persicaria spp., Commelina communis etc. in the annual stage (0∼1 years); Erigeron spp., Artemisia spp., Rubus crataegus etc. in the Erigeron-Artemisia stage (2∼6 years); Pinus densiflora, Salix spp., Miscanthus sinensis, Arundinella hirta etc. in the shrub - earlier tree stage (10∼25 years); P. densiflora, Quercus mongolica, Spodiopogon sibiricus etc. in the mid-tree stage (25∼50 years); Q. mongolica, Tripterigium regelii, Lespedeza maximowiczii, Carex siderosticta etc. in the latter tree stage (50∼80 years). In mid-tree stage, size-frequency distribution of P. densiflora was plotted as a platycurtic curve and that of Q. mongolica as a reverse J-shaped curve, which meant P. densiflora was thinned through interspecific competition and Q. mongolica was regenerated by itself in the later tree stage.

• 한국환경농학회지
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• 제34권3호
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• pp.238-243
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• 2015

BACKGROUND: Yeasts associated with fleabane flowers were identified using isolation methods previously applied in yeast biotechnology. A culture-based approach was required for isolation of many yeast strains associated with fleabane. METHODS AND RESULTS: We spread homogenized fleabane flowers onto GPY medium containing chloramphenicol, streptomycin, Triton X-100, and L-sorbose. We isolated 79 yeast strains from the flowers of wild fleabane, and identified the yeasts via phylogenetic analysis of isolates from agar plates. The yeast species included 39 isolates of Aureobasidium pullulans, 17 of the genus Candida, 14 of the genus Rhodosporidium, 6 of the genus Cryptococcus, and 3 of the genus Rhodotorula. CONCLUSION: Yeast isolates associated with fleabane flowers included A. pullulans (39 isolates) and other yeast species (40 isolates). Such yeast isolates may have biotechnological potential.