• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.2
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• pp.71-81
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• 2016

This text was experimented and investigated the optimum production time and germination characteristics of seed that collect in Korean Chungnam, in order to offer the basic informations for slope restoration and revegetation using Poaceae plant. Optimum time of seed production was mid-late October of Miscanthus sinensis, Pennisetum alopecuroides, early November to late October of Themeda triandra var. japonica and M. sacchariflorus, and mid November of Phragmites communis. Epiphytic amount of seed full ripe was the most times in mid October of M. sinensis, P. alopecuroides and M. sacchariflorus, early November of T. triandra var. japonica, and mid November of P. communis. Seed rate of maturity was the highest times in early November of M. sinensis, P. alopecuroides and M. sacchariflorus, and mid November of T. triandra var. japonica and P. communis. Germination rate by seed collection time was the highest times in early October of M. sacchariflorus, mid October of T. triandra var. japonica, early November of M. sinensis and mid November of P. communis. Germination days by seed production time was the shortest times in early November of M. sinensis and M. sacchariflorus, mid November of T. triandra var. japonica and P. communis. Optimum temperature of germination was $20{\sim}25^{\circ}C$ in M. sinensis and P. alopecuroides, $25^{\circ}C$ in T. triandra var. japonica, and $25{\sim}30^{\circ}C$ in P. communis.

• ALGAE
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• v.29 no.4
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• pp.249-265
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• 2014

As with land crops, cultivated algae are affected by various diseases ranging from large outbreaks of a disease to chronic epiphytes, which may downgrade the value of the final product. The recent development of intensive and dense mariculture practices has enabled some new diseases to spread much faster than before. A new disease is reported almost every year, and the impact of diseases is expected to increase with environmental change, such as global warming. We observed the incidence of diseases in two Pyropia sea farms in Korea from 2011 to 2014, and estimated the economic loss caused by each disease. Serious damage is caused by the oomycete pathogens, Pythium porphyrae and Olpidiopsis spp., which decreased the productivity of the Pyropia sea farms. In Seocheon sea farms, an outbreak of Olpidiopsis spp. disease resulted in approximately US $1.6 million in loss, representing approximately 24.5% of total sales during the 2012-2013 season. The damage caused by green-spot disease was almost as serious as oomycete diseases. An outbreak of green-spot disease in the Seocheon sea farms resulted in approximately US $1.1 million in loss, representing 10.7% of total sales in the 2013-2014 season in this area. However, the causative agent of green-spot disease is still not confirmed. "Diatom felt" is regarded as a minor nuisance that does not cause serious damage in Pyropia; however, our case study showed that the economic loss caused by "diatom felt" might be as serious as that of oomycete diseases. Bacteria and cyanobacteria are indigenous members of epiphytic microbial community on Pyropia blades, but can become opportunistic pathogens under suitable environmental conditions, especially when Pyropia suffers from other diseases. A regular acid wash of the Pyropia cultivation nets is the most common treatment for all of the above mentioned diseases, and represents approximately 30% of the total cost in Pyropia sea farming. However, the acid wash is ineffective for some diseases, especially for Olpidiopsis and bacterial diseases.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.133.1-133
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• 2003

Gummy stem blight, caused by Didymella bryoniae occurs exclusively on cucurbits. This fungus has been known not to produce its pycnidium in vitro unless irradiated. Through this study, we optimized cultural conditions for mass-production of pycnidiospore by Metal Halide Lamp irradiation. In brief, the mycelial was cultured at $26^{\circ}C$ on PDA, for 2 days under the darkness, and then the plate was illuminated with MH lamp continuously for 3-4 days at $26^{\circ}C$, a great number of pycnidia was simultaneously formed. Thus produced pycnidiospores were used as immunogen. From fusions of myeloma cell (v-653) with splenocytes from immunifed mice were car ried out. And, two hybridoma cell lines that recognized the immunogen Didymella bryoniae were obtained. One Monoclonal Antibody, Db1, recognized the supernatant and the other monoclonal antibody, Db15, recognized the spore. Two clones were selected which were used to produce ascite fluid two MAb Db1 and Db15, were immunotyped and identified as IgG1 and IgG2b, respectively. Titer of MAb Db1 and MAb Db15 was measured absorbance exceeded 0.5 even at a $10^{-5}$ dilution. The MAbs reacted positively with Didymella bryoniae but none reacted with other of fungi and CMV, CGMMV Sensitivity of MAb was precise enough to detect spore concentration as low as $10^{3}$ well by indirect ELISA characterization of the MAb Db1, Db15 antigen by heat and protease treatments show that the epitope recognized by the MAb Bb1, Db15 were a glycoprotein.

• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.205-211
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• 1996

The study was conducted to determine the effects of an optimum irrigation time as af­fected by the different soil textures in Dioscorea batatas DECNE. Water content change was decreased fol­lowing orders, non - irrigation > July 15 > Aug. 15 > irrigation of two times (July 15 following Aug. 15). It was appeared that sandy loam texture was sharply decreased compared with the loam texture in water loss. Dan-Ma was preemergenced about 6 to 8 days without soil texture. Epiphytic amounts in both Dan-Ma and Jang-Ma were about two times increased at tubberous fomation and enlargement stages comparing non - irrigation. In tuber yield without soil texture, Jang-Ma was increased compared with Dan-Ma and tuber yield in loam soil texture was promoted. As a result, it was concluded that sandy loam texture has more compatible than loam soil texture.

• Journal of Mushroom
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• v.8 no.4
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• pp.157-160
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• 2010

Agaricus blazei Murill is a important medicinal mushroom for a powerful immune system builder and tonic. Currently, it is known about a new disease phenomenon that appears to be occurring on a number of mushroom farms. We described a straightforward approach in which molecular methods was used to survey the presence of potentially endo- and epiphytic bacteria infected with the Agaricus blazei. The 16S rDNA was amplified with universal eubacterial primers directly from pure cultures of Agaricus blazei mycelium and fruit body. The 16S rDNA sequences were almost identical (96 to 97% similarity), and phylogenetic analysis placed them in a single unique rRNA branch belong to the uncultural bacterium phylogroup. PCR detection of uncultural bacterium in the malformed tissues of Agaricus blazei were carried out by using 16S rRNA sequenced specific probe. It was strongly amplified at the malformed pileus region of fruit body and also spore print was impossible.

• Korean Journal of Environmental Biology
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• v.23 no.2 s.58
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• pp.135-140
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• 2005

In order to construct an artificial cultivation of Nostoc flagelliforme on solid medium, we attempted to assess the viability of approaches, which utilized either BG-11 agar or sand medium using both sterile and non-sterile algal segments. In the trial in which the BG -11 agar medium was inoculated with the non-sterile algal segments, the algae exhibited the rapid growth in the initial 4 days of cultivation. However, after 4 days of cultivation, the growth rate of the algae slowed, and the algal growth was completely stopped by 7 days of cultivation. When the BG -11 medium was inoculated with the sterile algal segments, the algae exhibited the rapid growth for a longer period of 8 days, reaching a length of 24.9 mm. The growth rate during this period was measured to be $24.5\%$. After the 8 days of cultivation, the algal growth rate began to slow and had almost stopped by the 13 days of cultivation. On the other hand, when the sterile algal segments were inoculated onto a sand plate, the algal segments decomposed, reaching total decomposition after 11 days of cultivation. By way of contrast, the desiccation treatment samples continued to grow for 14 days of cultivation. After 14 days of cultivation, the algae achieved a length of 26.1 mm, with a growth rate of $30.6\%$. Our results indicate that periodic desiccation may constitute an effective strategy for the prevention of algal decomposition.

• Korean Journal of Environmental Biology
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• v.30 no.3
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• pp.272-279
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• 2012

This was the second study on the ecological characteristics of the epiphytes on seagrass leaf. The objective of this study was to understand the variation of epiphytes on seagrass leaf depending on the change of physico-chemical factors such as salinity, nutrients, and etc. This study showed the four results. 1) The eelgrass growth was influenced by water temperature, suggesting the positive correlation between eelgrass growth and water temperature. 2) The epiphytes growth on seagrass leaves did not show the correlation with water temperature, but negatively correlated with salinity. 3) The eelgrass growth decreased when the concentraion of nitrogen increased. 4) However, loads of epiphytes increased when the concentration of total nitogen (TN), nitrate ($NO_3^-$), and nitrite ($NO_2^-$) were high. This increase of epiphytes growth could be suggested in the cause-effect pathway of nutrient enrichment leading to seagrasses loss.

• ALGAE
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• v.25 no.3
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• pp.141-153
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• 2010

Seagrass epiphyte blooms potentially have important economic and ecological consequences in Tampa Bay, one of the Gulf of Mexico's largest estuaries. As part of a Tampa Bay pilot study to monitor the impact of environmental stresses, precise characterization of epiphyte diversity is required for efficient management of affected resources. Thus, epiphyte diversity may be used as a rational basis for assessment of ecosystem health. In May 2001, epiphytic species encompassing green, brown and red macroalgae were manually collected from dense and sparse seagrass beds of Thalassia testudinum and Syringodium filiforme. A total of 20 macroalgal epiphytes, 2 Chlorophyta, 2 Phaeophyta, and 16 Rhodophyta, were found on T. testudinum and S. filiforme seagrass at the four sampling sites (Bishop Harbor, Cockroach Bay, Feather Sound, and Mariposa Key). The Rhodophyta, represented by 16 species, dominated the numbers of species. Among them, the thin-crusted Hydrolithon farinosum was the most commonly found epiphyte on seagrass leaves. Species number, as well as species frequency of epiphytes, is higher at dense seagrass sites than sparse seagrass sites. Four attachment patterns of epiphytes can be classified according to cortex and rhizoid development: 1) creeping, 2) erect, 3) creeping & erect, and 4) erect & holding. The creeping type is characterized by an encrusting thallus without a rhizoid or holdfast base. Characteristics of the erect type include a filamentous thallus with or without a cortex, and a rhizoid or holdfast base. The creeping and erect type is characterized by a filamentous thallus with a cortex and rhizoid. A filamentous thallus with a cortex, holdfast base, and host holding branch is characteristics of the erect and holdfast attachment type. This study characterized each species found on the seagrass for epiphyte identification.

• Journal of Fisheries and Marine Sciences Education
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• v.25 no.6
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• pp.1336-1347
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• 2013

The marine algal flora assemblages settled on the artificial seaweed reefs in Jeju-do before and after rocky cleaning were investigated during summer and the early succession of seaweeds attached to the test panels on the structures of the artificial reef after rocky cleaning were observed by visual observations and under the dissecting microscope in order to evaluate the effects of this rocky cleaning operation. Species composition and algal biomass on the Bboolsamgak-typed seaweed artificial reefs before and after rocky cleaning were measured and compared before and after rocky cleaning. Before rocky cleaning in July 2012, only nine algal species had existed on the Bboolsamgak-typed seaweed artificial reefs, however, twenty algal species occurred on the seaweed artificial reefs after rocky cleaning in July 2013. Particularly, 15 species among them in July 2013 were not found in July 2012 and they seemed to occur newly on the artificial reefs. A large quantity of algal biomass increased in July 2013 after rocky cleaning. Based on the importance value, there was a drastic change in the dominant species between July 2012 and July 2013 after rocky cleaning. By dissecting micrographs, an epiphytic diatom, Licmophora sp. and flagellates, were observed on the 9th day. A chlorophyte, Ulothrix flacca was observed after 14 days, Ulva pertusa, Bryopsis pulmosa, crustose coralline algae, and Cladophora spp. were after one month. After two months, a significant species succession was observed from three previous chlorophyte species to crustose coralline algae existed as a dominant species on the panels. In this study, however, any brown algae were not found until three months in the early seaweed succession. All above results indicates that the rocky cleaning is a very efficient method for the establishment of macroalgal assemblages on the artificial reefs.

• ALGAE
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• v.24 no.4
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• pp.249-256
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• 2009

The occurrence of Laminarionema elsbetiae (Ectocarpaceae, Phaeophyceae), as epi-endophyte of Rhodymenia pseudopalmata (Rhodymeniales, Rhodophyta), described from Santa Isabel, Rawson, Argentina. L. elsbetiae grows in the host tissues forming epi-endophytic relationship in the epidermal, cortical and medullar layers. Epiphytic thalli of L. elsbetiae were unbranched filaments emerging from hostis surface. Reproductive structures of L. elsbetiae on the host were absent. On the contrary, free cultured individuals formed different reproductive structures. Macrozoosporangia containing a single large motile zoospore originated from vegetative cells, they were conical to cylindrical in shape, 30-50 ${\mu}m$ in length and 18-20 ${\mu}m$ in wide. Uniseriate plurilocular zoosporangia were cylindrical shape, 40 ${\mu}m$ in length and 10-13 ${\mu}m$ in wide. Sexual fusion was not seen. In mixed cultures of L. elsbetiae with R. pseudopalmata fronds, L. elsbetiae infected the host, grew as in natural host and, formed macrosporangia between host subcortical cells. Gametophytes of L. elsbetiae were filaments with diffuse growth, branched with a branch pattern alternate or opposite. Gametangia were plurilocular, uni or biseriate and lateral. When mature they contained 2 to 6 isogametes. The presence L. elsbetiae on R. pseudopalmata could be defined as an epi-endophytic relationship. The percentage of infection of R. pseudopalmata thalli by L. elsbetiae was 34%.A25% of the infected thalli presented a low, non-symptomatic level infection, whereas a 62% and a 13% of them exhibited respectively moderate and high indexes of infection.