• 대한의용생체공학회:의공학회지
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• 제15권2호
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• pp.217-224
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• 1994

In order to study the shape and dimensions of heart, a procedure to reconstruct a three dimensional left ventricular geometry from two dimensional echocardiographic images was studied including the coordinate transformation, curve fitting and interpolation utilizing three dimensional position registration arm. Nonlinear material property of the left ventricular myocardium was obtained by finite element method performed on the reconstructed geometry and by optimization techniques which compared the computer predicted 3D deformation with the experimentally determined deformation. Elastic modulus ranged from 3.5g/$cm^2$ at early diastole to l53g/$cm^2$ at around end diastole showing slightly nonlinear relationship between the modulus and the pressure. Afterwards using the obtained nonlinear material propertry the stress distribution related with oxyzen consumption rate was analyzed. The maximum and minimum of ${\sigma}_1$ (max. principal stress) occurred at nodes on the second level intersection points of x-axis with endocardium and with epicardium, respectively. And the tendency of the interventricular septum to be flattened was observed from the compressive ${\sigma}_1$ on the anterior, posterior nodes of left ventricle and from the most significant change of dimension in $D_{RL}$ (septal-lateral dimension of right ventricle).

• 대한수의학회지
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• 제38권4호
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• pp.843-852
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• 1998

From Jan. 1996 to Oct. 1997, 29 pigs with 40-70 days old showing dyspnea inappetite and polyserositis were collected and carried out necropsy, bacterial culture, histopathology, avidin biotin complex(ABC) stain, fluorescent antibody(FA) test, and electron microscopy. In the study, 4 strains from 3 pigs were isolated from meninges, pleura and synovial fluid and also were identified as Haemophilus parasuis serovar 5. Main histopathological lesions of 29 pigs with polyserositis were frequently composed of fibrinous peritonitis(27), pleurisy(22), interstitial pneumonia(21), fibrinous epicarditis(20), fibrinopurulent meningitis(8) and synovitis(4). By ABC stain, 11/29(37.9%) pigs with polyserositis were confirmed to have H parasuis serovar 5 in the cytoplasm of macrophages and neutrophils in cerebral meninges, epicardium, pleura surface of lung or serosa of spleen. ABC stain(20.8~40.0%) to detect H parasuis serovar 5 in tissues was more sensitive than bacterial culture(10.3%), but less sensitive than FA test(62.5%) using frozen tissues even though the result of 8 cases. By electron microscopy, a bacterium was also detected in the cytoplasm of macrophages in purulent exudate of cerebral meninges. Consequently, we confirmed that H parasuis serovar 5 has been involving to cause pigs with polyserositis and can be detected by FA and ABC stain as reliable diagnostic tools.

• Journal of Chest Surgery
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• 제19권2호
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• pp.225-231
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• 1986

Pericardial adhesions following open heart surgery pose a special problems, increasing the risk of cardiac reoperation because of the danger of damaging the heart, coronary artery and veins, or grafts and also the fibrous tissue may obliterate the pericardial space and eventually constrict the heart. This study was undertaken to evaluate the effect of intrapericardial urokinase and dextran 40 on the formation of pericardial adhesions in an animal model. latrogenic traumas on the pericardium were surgically induced in 30 rabbits, simulating injuries possible during actual surgery. In all rabbits, blood [1 ml] was obtained from an ear vessel and injected into the pericardium. Control group of ten rabbits did not receive any further medication, urokinase group of ten received 15, 000-20, 000 IU of urokinase, and remained ten received 1 ml of 10% dextran 40. All rabbits were sacrificed at 4 weeks. At autopsy, the development of adhesions were graded as none [Grade I], minimal [Grade II], moderate [Grade III], and severe [Grade IV]. Histological studies of the parietal pericardium and epicardium were performed. The results were as follows: 1. Group 1[Control group] showed minimal adhesion in 40%, moderate in 50%, and severe in 10% of the group. Sharp dissections were necessary in 60% of adhesions. 2. Group II [Dextran group] showed no adhesions in 20%, minimal in 60%, and moderate in 20% of the group. 3. Group III [Urokinase group] showed no adhesions in 40%, minimal in 40%, and moderate in 20% of the group. Considering in this group, the adhesion activity was significantly suppressed [60% adhesions] compared to the control group [100% adhesions] [P < 0.05]. 4. Histological findings revealed mild serosal fibrosis in none adherent group, loose fibrous connections between two layers of pericardium in minimal adhesion group, tight fibrous connections in moderate adhesion group, and marked fibrous thickening and close attachment of two surfaces were noted in severe adhesion group. These data have revealed the decreased incidence of pericardial adhesions with urokinase and dextran 40.

• Journal of Chest Surgery
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• 제40권12호
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• pp.863-866
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• 2007

실신을 주소로 내원한 64세 여자 환자에서 심 초음파상 좌심방 내에 $4.2{\times}2.4\;cm$의 다혈관성 종괴가 발견되었으며, 좌심방 혈관종 혹은 점액종을 의심하여 종양 절제술을 계획하였다. 술 전 시행한 관상동맥 조영술상 좌회선지에서 기시하여 종양으로 분포하는 내경 2 mm의 영양혈관이 발견되었다. 이에 종양 절제술을 시행함과 동시에 좌회선지에서 기시하는 영양혈관을 심외막에서 지혈클립으로 이중 결찰하여 좋은 결과를 얻었다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.871-872
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• 2005

• Asian-Australasian Journal of Animal Sciences
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• 제8권4호
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• pp.325-328
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• 1995

The experiment was conducted to study the comparative pathology of hemic system among three different broiler chicken strains i.e. Hubbard (H), Lohmann (L) and Indian River (IR) suffering from experimentally induced Hydropericardium Syndrome (HPS). For this 50 chicks of each strain were inoculated with HPS inoculum at the age of 21 days and other 50 chicks of each broiler strain were kept as uninoculated control. After slaughtering each bird of both groups was subjected to pathological examination of heart, spleen and aorta and also for determining routine haematological parameters. The maximum values of Total Erythrocyte Count (TEC), heterophils and thrombocytes and the minimum values of Total Leukocyte Count (TLC) and Hemoglobin (Hb) content were found in H broiler strain. Percentage of monocytes, basophils and eosinophils also showed maximum decrease in H broiler chicken strain. Gross pathological lesions in the inoculated birds revealed that the heart showed ballooning due to distention of pericardial sac with pericardial fluid. Haemorrhages on the epicardium and flabbiness of the myocardium. Under the microscope, degenerative changes in myocardial tissue were seen. Lesions in the spleen included splenomegaly and haemorrhagic spots. Aorta showed flabbiness of the wall and disruption of endothelium. It is concluded that there is a marked difference in susceptibility of HPS among three different commercial broiler strains. The Hubbard broiler strain is more susceptible which is followed by the Indian River and Lohmann respectively.

• 한국동물위생학회지
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• 제30권1호
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• pp.33-42
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• 2007

Fowl cholera(FC), which has been classified as reportable animal disease in Korea, occurred in the backyard farm located in Chungju, Chungbuk province. The farm was not ordinary domestic poultry farm but backyard farm with main cultivation of fruits. The farmer reported unusual mortality of chickens which were grazed in his own fruit farm without any poultry house. There was a lots of opportunities to contact with wild animals including wild birds freely. Clinical signs observed in the chickens were torticolis, mucous excretion from mouth and greenish diarrhea. On the necropsy we found multifocal necrosis on the surface of liver, hemorrhages in the epicardium and ovary and mucopurulent exudates in the joint. The causative agents was isolated from the liver of chickens collected from the farms and identified as Pasteurella multocida using biochemical tests of bacteria. Also, the isolated bacteria were inoculated into specific pathogen free chickens to evaluate the pathogenicity. High mortality and similar pathological lesions compared to those of chickens died in the farm were observed. We report the first case of fowl cholera of the chickens in Korea and further studies are needed to evaluate the serotype, genotype and pathogenicity of isolated Pasteurella multocida.

• 한국어류학회지
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• 제27권3호
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• pp.228-232
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• 2015

빙어의 심실 구조를 광학현미경과 주사전자현미경으로 관찰하였다. 빙어의 심장은 정맥동, 심방, 심실 및 동맥구가 연속적으로 배열되어 있었다. 심실의 외벽은 심내막, 심근층, 심외막하층 및 심외막으로 구분되었고, 심방과 심실 사이의 방실판막 및 심실과 동맥구 사이의 구실판막이 관찰되었다. 심실은 주머니 모양이었으며, 심근층은 해면성 심근으로만 구성되어 있었고 관상혈관은 분포하지 않았다. 심실에서 소주들은 중심 내강과 작은 내강들을 형성하였으며, 소주는 원기둥 모양이었다. 심외막하층, 방실판막 및 구실판막에서 아교질의 분포가 관찰되었으나 소주에서는 심실의 기저부에서 부분적으로 관찰되었다. 특히 소주들 사이에서는 심내막교가 관찰되었다. 이와 같은 결과는 육봉형 빙어가 서식환경 및 생활양식에 적응한 결과로 생각된다.

• Applied Microscopy
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• 제33권4호
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• pp.325-333
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• 2003

왕거미과 산왕거미(Araneus ventricosus)의 심관과 심근세포의 미세구조적 특성을 주사형 및 투과형 전자현미경으로 관찰하였다. 거미의 심관은 복부마디의 등쪽에 분포되어 있고, 심외막과 심근층으로 구성되어 있었다. 심근층의 근섬유들은 종축을 중심으로 나선상의 배열을 이루고 있었고, 내막이 없이 혈림프에 직접 노출되는 구조를 이루고 있었다. 심관의 외부 표면에서는 3쌍의 심문이 형성되어 있었고, 내강에서는 원형질혈구와 과립혈구, 그리고 편도혈구 등 다양한 유형의 혈구들이 관찰되었다. 특히 심관의 내강을 향해 돌출된 심근돌기 주위에서는 편도혈구들이 대부분을 차지하고 있었다. 편도혈구의 세포질에는 유리 리보조옴이 산재되어 있었고 핵에는 이질염색질과 인이 발달되어 있었으나, 심근층 조직과의 특이한 연접은 관찰되지 않았다. 심근층에는 횡문이 형성되어 있었고, Z-line을 중심으로 근절의 구조를 이룬 근원섬유의 주위에서는 미토콘드리아와 근소포체가 풍부하게 함유되어 있었다. 심관의 배면을 따라 뻗은 신경절의 축삭들이 심근세포와 신경근육간 연접부를 형성하고 있음이 관찰되었다.

• 한국동물위생학회지
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• 제37권3호
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• pp.213-218
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• 2014

In early April 2014, a month-old Alexandrine Paraqeet (Psittacula eupatria) that was raised in a domestic aviary located in Gyungju-si, Korea was suddenly died and submitted to Animal Disease Intervention Center, Kyungpook National University in order to diagnose the causative agent. In post-mortem examination, the bird had abnormally developed feathers on the neck and abdomen region and subcutaneous hemorrhages on the neck and cheek adjacent to the beak. At necropsy, the bird had hemorrhage on the muscle of the femoral region, ascites, multi-focal hemorrhages on the epicardium, and diffuse hemorrhages on the sub-serosa of proventriculus and gizzard, suggesting typical avian polyomavirus (APV) infection. The partial large tumor (T) antigen gene of APV was detected by PCR from tissues of the heart, lung, liver, kidney, proventriculus and feathers of the APV-suspected birds. However, other pathogenic virus-specific nucleic acid common with psittacine birds such as avian bornavirus, psittacine beak and feather disease virus and psittacid herpesvirus were not detected from the mixed tissue samples of the bird, indicating this case is due to single infection of APV. Nucleotide sequence analysis of the partially amplified large T antigen DNA was confirmed to have 99~100% homology with that of the previously reported APV strains. This case report describes the first detection of APV in Alexandrine Paraqeet in Korea.