• The Journal of Korean Society of Virology
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• v.29 no.3
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• pp.183-193
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• 1999

Human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein is synthesized as a 160 KDa precursor, gp160, that is cleaved by a cellular protease to form the gp120 and gp41 subunits. Mammalian expression vectors were designed that are capable of efficient expression of various mutant envelope glycoproteins derived from a molecular clone of HIV-1. To construct these vectors, one type of mutation was made at the gp120-gp41 cleavage site by oligonucleotide-directed mutagenesis. And another mutation was made to change amino acids in the membrane spanning region of HIV-1 gp41 important for membrane anchorage. Next, these two mutations were combined to generate a vector to have double mutations in cleavage site and membrane-spanning region. These mutants were transiently expressed in mammalian cells. The effect of these mutations on envelope glycoprotein synthesis, proteolytic processing and secretion was determined. In addition, cell surface expression and ability of the glycoprotein to induce syncytium formation were examined. This study provides a mammalian expression system that is capable of efficient expression and secretion of soluble gp160.

• Proceedings of the Korean Geotechical Society Conference
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• 2000.03b
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• pp.457-464
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• 2000

Stereographic method is a general and basic method to analyse sliding failure potential of rock slope. Region of failure analysis using stereographic method extend to curved slope from straight slope in this paper, Curved slope is defined as the multi-face slope with free surface more than two face and has different characteristics from straight single face slope. Individual daylight envelopes of free surfaces are combined into total daylight envelope of multi-face slope. So, sliding envelope of multi-face slope is the daylight envelope except friction cone. Specially, If only single joint set is developed in the slope, single plane sliding(or plane failure) is impossible in the single-face straight slope, but possible in the multi-face slope. In the multi-face slope with only one joint set, single plane sliding occurs when orientation of sliding plane is between two side slope orientation in the sliding envelope.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.45 no.12
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• pp.1084-1093
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• 2017

As the survivability of an aircraft in the battlefield becomes a critical issue, there is a growing need to improve the survivability of the aircraft. In this study, the survivability of an UCAV associated with plume IR signature was investigated. In order to analyze the survivability of the aircraft, the lock-on range and the lethal envelope, defined as the IR detection distance of the aircraft and the range of shooting down by the missile, respectively, were first introduced. Further, a method to calculate the lethal envelope for the scenario of surface-to-air missiles including the vertical plane was developed. The study confirmed that the red zone of an UCAV shows a substantial difference in the zone size as well as the characteristics in the upward and downward directions.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.11a
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• pp.502-505
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• 2006

Acoustic holography allows us to predict spatial pressure distribution on any surface of interest from measured hologram. It is noteworthy that the data size is so huge that it takes long time to calculate pressure field. Moreover the reconstructed pressure field is frequently too complicated to get what we want to know. One possible candidate is complex envelope. Complex envelope in time domain is well known and widely used in various engineering field. We have attempted to extend this method to space domain, so that we can have rather simple spatial pressure picture that provides information we need, for example, where sound sources are. First we start with the simplest case. We examine the complex envelope of a plane wave on both space and wave number domain. Then we extend to monopole case. Holographic reconstructed sound field on the monopole is processed according to what we propose. We demonstrate how this method provides better picture for analyzing the sound field.

• Journal of Ship and Ocean Technology
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• v.8 no.3
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• pp.18-25
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• 2004

Sound reception system is required to detect the sound and the quadrantal direction of the other ship's horn sound, to overcome the effects of enclosed wall for navigation space, functioning as a sound barrier. However, the realized systems can only provide quadrantal information of the other ship. This paper presents a new arrangement of microphones, having geometrically symmetric deployment with the same distances between sensors and the same angles between adjacent sensors with respect to the geometrical center. The sound pressures received at microphones are transformed into the related envelope signals by applying Hilbert transform. The time delays between microphones are estimated by the correlation functions between the derived envelope signals. This envelope base processing mitigates the noises related to the reflection by ship and sea surface. Then, the directional information is easily defined by using the estimated time delays. The suggested method is verified by the generated signals using boundary element method for a small ship model with sea surface wave. The estimated direction is quite similar to the true one and therefore the proposed approach can be used as an efficient sound reception system.

• Journal of the Society of Naval Architects of Korea
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• v.54 no.4
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• pp.301-311
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• 2017

Safety operational envelope (SOE) is the area which guarantees the safety of a submarine from the accident such as jamming and flooding. The maximum safe depth is set to prevent the damage to the hull from increasing water pressure with depth. A minimum safety depth is set to prevent a submarine from the exposure above the free surface and collision against surface ship. The prediction method for the SOE in the design phase is needed to operate the submarine safely. In this paper, the modeling and calculation methods of the SOE are introduced. Main ballast tank blowing modeling and propeller force modeling are conducted to simulate the accidents and the recovery process. The SOEs are established based on the crash stop and emergency rising maneuver simulation. From the simulation results, it can be known that the emergency rising maneuver is more effective recovery action than the crash stop.

• Journal of Magnetics
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• v.21 no.3
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• pp.356-363
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• 2016

To improve the sensing accuracy of the newly developed magnetically suspended sensitive gyroscope (MSSG), it is necessary to analyze the causes of drift error. This paper build the models of disturbing torques generated by stator assembly errors based on the geometric construction of the MSSG with double spherical envelope surfaces, and further reveals the generation mechanism of the drift error. Then the drift error from a single stator magnetic pole is calculated quantitatively with the established model, and the key factors producing the drift error are further discussed. It is proposed that the main approaches in reducing the drift error are guaranteeing the rotor envelope surface to be an ideal spherical and improving the controlling precision of rotor displacement. The common problems associated in a gyroscope with a spherical rotor can be effectively resolved by the proposed method.

• Applied Microscopy
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• v.30 no.4
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• pp.321-326
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• 2000

The ultrastructure of the fertilized egg envelope from pale chub , Zacco platypus belong to Cyprinidae was studied using scanning and transmission electron microscopes. The fertilized egg was an adhesive type, have a single micropyle resembling the pathway of sperm in the area of the animal pole. The micropyle was surrounded by 5 peaks of hill. An outer surface of the fertilized egg envelope was arranged by pore canals irregularly. In section of fertilized egg, the egg envelope consistes of three layers, an outer adhesive layer, a middle electron dense layer and an inner lamellae layer consisting of 9 layers. These ultrastructural characters of fertilized egg envelope from pale chub can be utilized in taxonomy of teleost.

• Applied Microscopy
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• v.26 no.3
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• pp.277-291
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• 1996

The structures of the egg envelope in fertilized eggs of three species of characidae, head and tail light fish (Hemigrammus ocellifer), black tetra (Gymnocorymbus ternetzi), and buenos aires tetra (Hemigrammus caudovittatus) were studied using light and electron microscopes. The fertilized eggs in all species were colorless, transparent, spherical and non-floted type. The egg envelopes have a single micropyle resembling the pathway of sperm in the area of the animal pole. The micropyle was surrounded by protruded lines of the egg envelope in a radiated form. Egg envelopes of fertilized eggs in both head and tail light fish and buenos aires tetra consisted of three distinct layers; an outer layer, a middle layer and an inner layer. And that of blacktetra consisted of two layers; an outer layer and an inner layer. Also, an outer layers of both head and tail light fish and black tetra were adhesive types but, in that of buenous aires tetra was non-adhesive type. An outer surface of egg envelope in black tetra was arranged by pores regularly. In that of head and tail light fish and buenos aires tetra have a rough side. An inner layer of egg envelope in fertilized eggs consisted of lamellae alternating with interlamellae of lower electron density; an inner layer of fertilized eggs in head and tail light fish consisted of three layers, that of black tetra was four layers, and that of buenos aires tetra was five layers.

• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.470-478
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• 2012

Recent genome comparisons of E. coli B and K-12 strains have indicated that the makeup of the cell envelopes in these two strains is quite different. Therefore, we analyzed and compared the envelope proteomes of E. coli BL21(DE3) and MG1655. A total of 165 protein spots, including 62 nonredundant proteins, were unambiguously identified by two-dimensional gel electrophoresis and mass spectrometry. Of these, 43 proteins were conserved between the two strains, whereas 4 and 16 strain-specific proteins were identified only in E. coli BL21(DE3) and MG1655, respectively. Additionally, 24 proteins showed more than 2-fold differences in intensities between the B and K-12 strains. The reference envelope proteome maps showed that E. coli envelope mainly contained channel proteins and lipoproteins. Interesting proteomic observations between the two strains were as follows: (i) B produced more OmpF porin with a larger pore size than K-12, indicating an increase in the membrane permeability; (ii) B produced higher amounts of lipoproteins, which facilitates the assembly of outer membrane ${\beta}$-barrel proteins; and (iii) motility- (FliC) and chemotaxis-related proteins (CheA and CheW) were detected only in K-12, which showed that E. coli B is restricted with regard to migration under unfavorable conditions. These differences may influence the permeability and integrity of the cell envelope, showing that E. coli B may be more susceptible than K-12 to certain stress conditions. Thus, these findings suggest that E. coli K-12 and its derivatives will be more favorable strains in certain biotechnological applications, such as cell surface display or membrane engineering studies.