• Title/Summary/Keyword: Enumeration method

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Enumeration and Activity of Methanogenic Microorganisms of th Anaerobic Digestion Process

  • Lee, Kwang-Ho
    • Korean Journal of Hydrosciences
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    • v.2
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    • pp.115-126
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    • 1991
  • The anaerobic digester with sludge from sewage treatment plant was operated in the laboratory for two year to investigate the enumeration and activity of methanogenic microorganisms. In this experimental study, the effects of HRT on the degradation characteristics of organic materials and on the number of methanogenic bacteria produced were investigated. By making the media with the repeated wxperiment, the number and activity of methanogenic bacteria were measured. The increase of the removal rate of organic acid in the digester was oberved at HRT of 2 days. The total number of methane forming bacteria estimated by the MPN method showed 2.3 $\times$ $ at HRT of 3 days, 7$\times$$ of 5 days and 7.9$\times$$ $/ml of 10 days. The optimum incubation time for measuring the number of methanogenic bacteria was found as more than four weeks. The PMA revealed 161ml CH$/l day at HRT of 10 days and the PUA 290mg COD/l day. At the incubation time 4.3 days, the maximum value of CH$ *59.1%) was found. At this time, $ was found as 15.3% and $ 25.6%.

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Optimal design of reinforced concrete beams: A review

  • Rahmanian, Ima;Lucet, Yves;Tesfamariam, Solomon
    • Computers and Concrete
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    • v.13 no.4
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    • pp.457-482
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    • 2014
  • This paper summarizes available literature on the optimization of reinforced concrete (RC) beams. The objective of optimization (e.g. minimum cost or weight), the design variables and the constraints considered by different studies vary widely and therefore, different optimization methods have been employed to provide the optimal design of RC beams, whether as isolated structural components or as part of a structural frame. The review of literature suggests that nonlinear deterministic approaches can be efficiently employed to provide optimal design of RC beams, given the small number of variables. This paper also presents spreadsheet implementation of cost optimization of RC beams in the familiar MS Excel environment to illustrate the efficiency of the exhaustive enumeration method for such small discrete search spaces and to promote its use by engineers and researchers. Furthermore, a sensitivity analysis is performed on the contribution of various design parameters to the variability of the overall cost of RC beams.

A PATH ENUMERATION APPROACH FOR CRITICAL ANALYSIS IN PROJECT NETWORKS WITH FUZZY ACTIVITY DURATIONS

  • Siamak Haji Yakchali
    • International conference on construction engineering and project management
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    • 2011.02a
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    • pp.575-581
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    • 2011
  • A novel approach for analysis of criticality with respect to path and to activity in networks with fuzzy activity durations is proposed. After recalling the Yager ranking method, the relative degree of criticality of activities and paths are defined. An efficient algorithm based on path enumeration to compute the relative degree of criticality of activities and paths in networks with fuzzy durations is proposed. Examples of former researches are employed to validate the proposed approach. The proposed algorithm has been tested on real world project networks and experimental results have shown that the algorithm can calculate the relative degree of criticality of activities and paths in a reasonable time.

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An Exact Algorithm for the Aircraft Scheduling Problem (비행기 일정계획 문제를 위한 최적해법)

  • 기재석
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.15 no.25
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    • pp.91-95
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    • 1992
  • The aircraft schedule is the central of an airline's planning process, aimed at optimizing the deployment of airline's resources in order to maximize profits In this paper, the aircraft schedule is formulated as an integer programming model and the exact algorithm hared on enumeration method is proposed.

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Model Predictive Torque Control of Surface Mounted Permanent Magnet Synchronous Motor Drives with Voltage Cost Functions

  • Zhang, Xiaoguang;Hou, Benshuai;He, Yikang;Gao, Dawei
    • Journal of Power Electronics
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    • v.18 no.5
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    • pp.1369-1379
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    • 2018
  • In this paper, a model predictive torque control (MPTC) without the use of a weighting factor for surface mounted permanent-magnet synchronous machine (SPMSM) drive systems is presented. Firstly, the desired voltage vector is predicted in real time according to the principles of deadbeat torque and flux control. Then the sector of this desired voltage vector is determined. The complete enumeration for testing all of the feasible voltage vectors is avoided by testing only the candidate vectors contained in the sector. This means that only two voltage vectors in the sector need to be tested for selecting the optimal voltage vector in each control period. Thus, the calculation time can be reduced when compared with the conventional enumeration method. On the other hand, a novel cost function that only includes the dq-axis voltage errors between the desired voltage and candidate voltage is designed to eliminate the weighting factor used in the conventional MPTC. Thus, the control complexity caused by the tuning of the weighting factor is effectively decreased when compared with the conventional MPTC. Simulation and experimental investigation have been carried out to verify the proposed method.

Rapid Enumeration of Listeria monocytogenes in Pork Meat Using Competitive PCR

  • Lim, Hyung-Kun;Hong, Chong-Hae;Choi, Weon-Sang
    • Food Science and Biotechnology
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    • v.14 no.3
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    • pp.387-391
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    • 2005
  • Competitive polymerase chain reaction (cPCR) was used to develop a direct enumeration method of Listeria monocytogenes in pork meat. Pork meat was artificially inoculated with L. monocytogenes and DNA was extracted using guanidine thiocyanate-phenol-chloroform and subjected to PCR amplification. Sixteen primer sets for L. monocytogenes hlyA gene were tested for sensitive detection and the DG69/DG74 primer set was selected. The detection limit achieved with this primer set was as low as 860 colony-forming units (cfu) per 0.1 g of pork meat. When the samples were cultured at $30^{\circ}C$ for 16 hr in Brain Heart Infusion (BHI) medium, even a single bacterium could be detected with this primer set by PCR. For cPCR, the hlyA gene, which features a 148 bp-deletion, was cloned in the pGEM-4Z vector. A known amount of competitor DNA which has the same primer binding sites was co-amplified with L. monocytogenes total DNA from the artificially inoculated pork meat. The cell-number determined by cPCR was approximately equal to cfu from the Most Probable Number (MPN) method. The whole procedure took only 5 hr.

Methods Used in Determining Enumeration Districts in the 2005 Population and Housing Census (2005년 인구주택총조사의 조사구 설정 방법)

  • Lee Myung-Jin;Seo U-Seok;Byun Mi-Ree;Lee Kun
    • Survey Research
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    • v.7 no.1
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    • pp.109-129
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    • 2006
  • This paper describes how the National Statistics Office Prepared Population and Housing Census in 2005. We focus on the methods of identifying living quarters and determining the enumeration districts. In the absence of the supports of the regional administrative offices, caused by the regulation change in 1999, the NSO devised several new techniques, such as using previous census DB, administrative records, GIS, and etc.. However, not-well-organized administrative system of the regional offices, particularly in terms of the use of IT technology, becomes one of the major bottleneck for identifying living quarters. In order for better preparation of Census, we seem to concern about not only the NSO's efforts to improve survey methods, but also the administrative system of the regional offices. This may indicates that we are at the stake of moving towards a more networked administrative system beyond the present organizational boundaries.

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Enumeration of Weissella cibaria phage with cytometry, epifluorescence microscopy, and plaque assay (유세포분석기, 형광현미경, 용균반검사 분석을 이용한 Weissella cibaria 박테리오파지 정량분석 및 상관관계분석)

  • Park, Won Jeong;Lim, Ga-Yeon;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.50 no.2
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    • pp.244-247
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    • 2018
  • Quantitative analysis for non-host infection bacteriophage was conducted for their enumeration. Flow cytometry and epifluorescence microscopy (EPM) were selected as counting methods. Correlation analysis was performed based on the plaque assay method on the existing host infection and consisted of Pearson correlation statistical analysis, regression analysis, and difference analysis. Analyses of 12 samples with flow cytometry and plaque assay methods showed that there was a correlation of 96.7% with Pearson correlation value r=0.967, $R^2$ 0.9352, and difference value of 1.063. Analyses of 12 samples with EPM and plaque assay methods showed that there was a correlation of 99.0% with Pearson correlation value r=0.990, $R^2$ 0.9811, and difference value of 1.605. Therefore, flow cytometry and epifluorescence microscopy would be effective for enumeration of Weissella cibaria bacteriophage with plaque assay.

Estimating Price Elasticities of Domestic Air Transport Demand by Stated Preference Technique (Staled Preference 방법론에 의한 국내선 항공수요의 가격탄력성 추정)

  • 이성원;이영혁;박지형
    • Journal of Korean Society of Transportation
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    • v.18 no.1
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    • pp.27-34
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    • 2000
  • This study analyzes the price elasticities of airline Passenger demand through the 'Stated Preference' technique which uses survey data. Because the domestic airfare has been regulated by the government. it is not easy to derive Price elasticity through the usual regression analysis with aggregate data and thus a special methodology is required for elasticity estimation. Therefore, in this study we estimated the Price elasticities of domestic air passenger demand and the modal share change rates to the alternative modes with logit model and sample enumeration method, by analyzing the survey data on air Passengers' demand behavior about the mode choice between air-rail. air-bus, and air-car. As the results, the estimated price elasticities are in the range of -0.6~-0.9, and rail is mainly chosen as an alternative mode. bus is chosen Partly, and car is barely used.

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Development of a Proficiency Test Specimen for Enumerating Escherichia coli in Molluscan Bivalve Shellfish

  • Kwon, Ji-Young;Song, Ki-Cheol;Son, Kwang-Tae;Yu, Hong-Sik;Kim, Poong-Ho;Shin, Soon-Bum;Mok, Jong-Soo;Lee, Ka-Jeong;Oh, Eun-Gyoung;Yoon, Ho-Dong;Lim, Keun-Sick;Kim, Young-Mog
    • Fisheries and Aquatic Sciences
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    • v.14 no.3
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    • pp.226-229
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    • 2011
  • This study was conducted to develop a proficiency test (PT) specimen for the enumeration of Escherichia coli, a sanitary indicative bacterium, in molluscan bivalve shellfish. The common mussel Mytilus edulis was chosen as a matrix for the PT specimen. Mussels were artificially bioaccumulated for approximately 3 h with E. coli culture. After determining the homogeneity of samples, samples were distributed to 17 participants involved in the proficiency testing program. The enumeration of E. coli was performed by the most probable number method of the American Public Health Association. Statistical evaluations of the results obtained from inter- and intra-laboratory variation indicated no significant differences in the accuracy of these techniques between participants, indicating z-scores of ${\leq}{\pm}2$ and suggesting that preparation of the PT specimen for enumerating E coli in shellfish was successful.