• The Korean Journal of Mycology
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• v.9 no.4
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• pp.193-197
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• 1981

Nematode destroying-fungi from ginseng field were isolated and then identified on 2% wateragar and malt extract agar media. 1. Six strains of Arthrobotrys sp. and three strains of Harposporium sp. were isolated and identified. 2. Arthrobotrys sp. formed trapping apparatus when they were cultured with Nematode and appeared to be destroying fungi. 3. Harposporium sp. appeared to be endoparasitic fungi. 4. Both Arthrobotrys sp. and Harposporium sp. were grown well on nutrient agar and malt extract agar.

• Journal of Life Science
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• v.20 no.5
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• pp.644-648
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• 2010

The influence of nematodes on nematophagous fungi has seldom been investigated. In the present study, the influence of pinewood nematode on its endoparasitic fungus, Esteya vermicola, was investigated systemically. Although both nematodal metabolite and nematodal homogenate could stimulate and speed up the growth of E. vermicola, the impact of nematodal metabolite was slightly higher than that of nematodal homogenate. In addition, a method was developed to investigate the influence of volatiles, discharged by pinewood nematodes in their metabolic process, on the growth of E. vermicola. Reproductive results were given and confirmed that nematodal volatiles have no influence on the cell growth of E. vermicola. This study may provide information for the application of E. vermicola as biological control agent of pinewood nematode.

• Korean Journal of Environmental Biology
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• v.41 no.4
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• pp.657-665
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• 2023

The root-lesion nematode Pratylenchus spp. is the most important plantparasitic nematode due to its worldwide distribution, wide host ranges, and migratory endoparasitic characteristics. One population of Pratylenchus collected from the giant pussy willow (Salix chaenomeloides Kimura) in the Andong area as part of a nematode survey in Korea was characterized morphologically and by molecular methods. The analysis of morphological measurements and morphometric characteristics, as well as DNA sequencing of the rRNA large subunit (LSU) D2/D3 expansion segments and the internal transcribed spacer (ITS) gene sequence, confirmed the identity of this population as P. hippeastri. This study is the first report of P. hippeastri associated with Salix chaenomeloides in Korea and worldwide. Further studies on distribution and pathogenicity in different P. hippeastri host crops, such as grapevines, strawberries, and apples, are necessary. The taxonomic keys to 16 Pratylenchus species in Korea are provided.

• Korean Journal of Plant Resources
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• v.37 no.4
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• pp.314-320
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• 2024

To speed up the extract of endoparasitic nematodes from roots, four extraction methods with or without root-grinding were compared; 1) immersion, 2) immersion + Air, 3) Oostenbrink dish, and 4) Mistifier. The experiments were conducted for nine days by using perilla roots infested with Pratylenchus vulnus. Root-lesion nematodes continuously extracted from perilla roots during the experiments and as much as 3-10% in 9^th days. The total number of nematodes extracted from 2 g of perilla root in nine days were varied among methods (379-1,824 nematodes); the most nematodes were extracted by root grinding + immersion + air (1,824) and the root-grinding + mistifier method (1,349) (p = 0.05). In the first two days of extraction, root-grinding + mistifier extracted the most nematodes (725 nematodes), followed by root-grinding + immersion + Air (555 nematodes), and root-grinding + Oostenbrink dish (421 nematodes). Root-grinding effected as much as 16-108% more nematodes extraction when compared to without root-grinding (p = 0.01).