Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
권호 표지
DOI QR코드

DOI QR Code

Morphological and molecular characterization of root-lesion nematode Pratylenchus hippeastri from Korea

  • Sungchan Huh (Department of Plant Bioscience, Pusan National University) ;
  • Namsook Park (Life and Industry Convergence Research Institute, Pusan National University) ;
  • Heonil Kang (Forest Entomology and Pathology Division, National Institute of Forest Science) ;
  • Changhwan Bae (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Insoo Choi (Department of Plant Bioscience, Pusan National University)
  • Received : 2023.11.30
  • Accepted : 2023.12.22
  • Published : 2023.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

The root-lesion nematode Pratylenchus spp. is the most important plantparasitic nematode due to its worldwide distribution, wide host ranges, and migratory endoparasitic characteristics. One population of Pratylenchus collected from the giant pussy willow (Salix chaenomeloides Kimura) in the Andong area as part of a nematode survey in Korea was characterized morphologically and by molecular methods. The analysis of morphological measurements and morphometric characteristics, as well as DNA sequencing of the rRNA large subunit (LSU) D2/D3 expansion segments and the internal transcribed spacer (ITS) gene sequence, confirmed the identity of this population as P. hippeastri. This study is the first report of P. hippeastri associated with Salix chaenomeloides in Korea and worldwide. Further studies on distribution and pathogenicity in different P. hippeastri host crops, such as grapevines, strawberries, and apples, are necessary. The taxonomic keys to 16 Pratylenchus species in Korea are provided.

Keywords

Acknowledgement

This work was carried out with the support of "Cooperative research Program for Agriculture Science and Technology Department", Rural Development Administration (project number PJ01565402), Republic of Korea.

References

  1. Bogale M, B Tadesse, RH Nuaima, B Honermeier, J Hallmann and P DiGennaro. 2021. Morphometric and molecular diversity among seven European isolates of Pratylenchus penetrans. Plants 10:674. https://doi.org/10.3390/plants10040674
  2. Brenes-Campos R, LA Nunez-Rodriguez, L Flores-Chaves and DA Humphreys-Pereira. 2022. New record of the amaryllis lesion nematode, Pratylenchus hippeastri associated with strawberry in the Central Region of Costa Rica. Nematropica 51:131-136.
  3. Castillo P and N Vovlas. 2007. Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, Biology, Pathogenicity and Management. Nematology, Monographs and Perspectives 6(DJ Hunt and RN Perry eds.). Brill. Leiden, Netherlands. https://doi.org/10.1163/ej.9789004155640.i-523
  4. Chen WJ, T Bian, R Wu, XJ Lin, MZ Zhang, Y Wu, XY Wu and X Chen. 2014. Root-lesion nematode, Pratylenchus hippeastri, on Acer palmatum imported from Japan. Acta Agric. Zhejiangensis 26:708-713.
  5. Choi DR, JK Lee, BY Parte, HR Han and YE Choi. 2006. A new and one unrecorded species of Pratylenchus from Korea (Nematoda: Pratylenchidae). J. Asia-Pac. Entomol. 9:5-9. https://doi.org/10.1016/S1226-8615(08)60269-1
  6. De Luca F, A Troccoli, LW Duncan, SA Subbotin, L Waeyenberge, DL Coyne, FC Brentu and RN Inserra. 2012. Pratylenchus speijeri n. sp. (Nematoda: Pratylenchidae), a new root-lesion nematode pest of plantain in West Africa. Nematol. 14:987-1004. https://doi.org/10.1163/156854112X638424
  7. De Luca F, A Troccoli, LW Duncan, SA Subbotin, L Waeyenberge, M Moens and RN Inserra. 2010. Characterisation of a population of Pratylenchus hippeastri from bromeliads and description of two related new species, P. floridensis n. sp. and P. parafloridensis n. sp., from grasses in Florida. Nematol. 12:847-868. https://doi.org/10.1163/138855410X495809
  8. Eo J, MH Kim, HK Nam, SI Kwon and YJ Song. 2018. Effect of organic waste application on soil chemical properties and organisms under Zelkova serrata cultivation. Korean J. Environ. Biol. 36:471-478. https://doi.org/10.11626/KJEB.2018.36.4.471
  9. Franklin MT and JB Goodey. 1949. A cotton blue-lactophenol technique for mounting plant-parasitic nematodes. J. Helminthol. 23:175-178. https://doi.org/10.1017/S0022149X0003251X
  10. Gu JF, N Wang, J He and WJ Chen. 2014. Identification of Pratylenchus hippeastri from imported seedlings. Plant Quarantine 4:41-46.
  11. Handoo ZA, AM Skantar, MR Kantor, SL Hafez and MN Hult. 2020. Molecular and morphological characterization of the amaryllis lesion nematode, Pratylenchus hippeastri (Inserra et al. 2007), from California. J. Nematol. 52:1-5. https://doi.org/10.21307/jofnem-2020-058
  12. Handoo ZA, G Yan, MR Kantor, D Huang, IA Chowdhury, A Plaisance, GR Bauchan and JD Mowery. 2021. Morphological and molecular characterization of Pratylenchus dakotaensis n. sp. (Nematoda: Pratylenchidae), a new root-lesion nematode species on soybean in North Dakota, USA. Plants 10:168. https://doi.org/10.3390/plants10010168
  13. Inserra RN, A Troccoli, U Gozel, EC Bernard, D Dunn and LW Duncan. 2007. Pratylenchus hippeastri n. sp. (Nematoda: Pratylenchidae) from amaryllis in Florida with notes on P. scribneri and P. hexincisus, Nematol. 9:25-42. https://doi.org/10.1163/156854107779969754
  14. Kang H, G Eun, J Ha, Y Kim, N Park, D Kim and I Choi. 2016. New cyst nematode, Heterodera sojae n. sp. (Nematoda: Heteroderidea) from soybean in Korea. J. Nematol. 48:280-289. https://doi.org/10.21307/jofnem-2017-036
  15. Kim D and JY Chun. 2014. Identification of root-lesion nematode (Pratylenchidae: Pratylenchus) intercepted on imported plants. Korean J. Appl. Entomol. 53:51-57. https://doi.org/10.5656/KSAE.2013.10.1.083
  16. Kim JH, YH Jeon, H Park, BD Lee, DH Cho, BY Park, Z Khan and YH Kim. 2006. The root-lesion nematode, Pratylenchus subpenetrans, on ginseng (Panax ginseng) in Korea. Nematol. 8:637-639. https://doi.org/10.1163/156854106778613949
  17. Knoetze R, E van den Berg, C Girgan and L van der Walt. 2019. First report of the root lesion Nematode, Pratylenchus hippeastri, on apple in South Africa. J. Plant Dis. Prot. 126:607-609. https://www.jstor.org/stable/48699416
  18. Ko HR, E Park, EH Kim, SK Park, H Kang and B Park. 2021. Screening for resistance of Brassicaceae plant resources to clover cyst nematode. Korean J. Environ. Biol. 39:329-335. https://doi.org/10.11626/KJEB.2021.39.3.329
  19. NIBR. 2010. The Genetic Evaluation of Important Biological Resources. Pub. No. 11-1480592-000125-01. National Institute of Biological Resource. Incheon, Korea.
  20. Park BY. 1996. Taxonomical study of Pratyelchus spp. in Korea. MS Thesis. Konkuk University. Seoul, Korea.
  21. Park BY, DR Choi, JK Lee, YE Choi and GH Shin. 2002. Unrecorded species of Pratylenchus Ioosi Loof (Tylenchida: Pratylenchidae) from tea in Korea. Korean J. Appl. Entomol. 41:299-303.
  22. Park SN, YN Youn, JK Lee and BY Park. 2009. Survey of plant parasitic nematode in Citrus fruit orchards in Korea. Korean J. Appl. Entomol. 48:535-539.
  23. Seinhorst JW. 1959. A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematol. 4:67-69. https://doi.org/10.1163/187529259X00381
  24. Shokoohi E, J Abolafia, PW Mashela and N Divsalar. 2019. New data on known species of Hirschmanniella and Pratylenchus (Rhabditida, Pratylenchidae) from Iran and South Africa. J. Nematol. 51:1-26. https://doi.org/10.21307/jofnem-2019-041
  25. Subbotin SA, D Sturhan, VN Chizhov, N Vovlas and JG Baldwin. 2006. Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences. Nematol. 8:455-474.
  26. Subbotin SA, EJ Ragsdale, T Mullens, PA Roberts, M Mundo-Ocampo and JG Baldwin. 2008. A phylogenetic framework for root lesion nematodes of the genus Pratylenchus (Nematoda): Evidence from 18S and D2-D3 expansion segments of 28S ribosomal RNA genes and morphological characters. Mol. Phylogenet. Evol. 48:491-505. https://doi.org/10.1016/j.ympev.2008.04.028
  27. Tamura K, G Stecher and S Kumar. 2021. MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biol. Evol. 38:3022-3027. https://doi.org/10.1093/molbev/msab120
  28. Wang H, K Zhuo and J Liao. 2016. Morphological and molecular characterization of Pratylenchus hippeastri, a new record of root lesion nematode associated with apple in China. Pakistan J. Zool. 48:665-671.