• Title/Summary/Keyword: Elasticity test

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Development of Hair Keratin Protein to Accelerate Oral Mucosal Regeneration

  • So-Yeon Kim
    • Journal of dental hygiene science
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    • v.23 no.4
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    • pp.369-377
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    • 2023
  • Background: In this study, we investigated the potential use of keratin for oral tissue regeneration. Keratin is well-known for its effectiveness in skin regeneration by promoting keratinization and enhancing the elasticity and activity of fibroblasts. Because of its structural stability, high storability, biocompatibility, and safety in humans, existing research has predominantly focused on its role in skin wound healing. Herein, we propose using keratin proteins as biocompatible materials for dental applications. Methods: To assess the suitability of alpha-keratin protein as a substrate for cell culture, keratin was extracted from human hair via PEGylation. Viabilities of primary human gingival fibroblasts (HGFs) and human oral keratinocytes (HOKs) were assessed. Fluorescence immunostaining and migration assays were conducted using a fluorescence microscope and confocal laser scanning microscope. Wound healing and migration assays were performed using automated software to analyze the experimental readout and gap closure rate. Results: We confirmed the extraction of alpha-keratin and formation of the PEG-g-keratin complex. Treatment of HGFs with keratin protein at a concentration of 5 mg/ml promoted proliferation and maintained cell viability in the test group compared to the control group. HOKs treated with 5 mg/ml keratin exhibited a slight decrease in cell proliferation and activity after 48 hours compared to the untreated group, followed by an increase after 72 hours. Wound healing and migration assays revealed rapid closure of the area covered by HOKs over time following keratin treatment. Additionally, HOKs exhibited changes in cell morphology and increased the expression of the mesenchymal marker vimentin. Conclusion: Our study demonstrated the potential of hair keratin for soft tissue regeneration, with potential future applications in clinical settings for wound healing.

Investigation of the Cryogenic Performance of the High Density Polyurethane Foam (고밀도 폴리우레탄 폼의 극저온 성능 분석)

  • Jeong-Hyeon Kim;Jeong-Dae Kim;Tae-Wook Kim;Seul-Kee Kim;Jae-Myung Lee
    • Journal of the Korean Society of Industry Convergence
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    • v.26 no.6_3
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    • pp.1289-1295
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    • 2023
  • Polyurethane foam insulation required for storing and transporting cryogenic liquefied gas is already widely used as a thermal insulation material for commercial LNG carriers and onshore due to its stable price and high insulation performance. These polyurethane foams are reported to have different mechanical performance depending on the density, and the density parameter is determined depending on the amount of the blowing agent. In this study, density-dependent polyurethane foam was fabricated by adjusting the amount of blowing agent. The mechanical properties of polyurethane foam were analyzed in the room temperature and cryogenic temperature range of -163℃ at 1.5 mm/min, which is a quasi-static load range, and the cells were observed through microstructure analysis. The characteristics of linear elasticity, plateau, and densification, which are quasi-static mechanical behaviors of polyurethane foam, were shown, and the correlation between density and mechanical properties in a cryogenic environment was confirmed. The correlation between mechanical behavior and cell size was also analyzed through SEM morphology analysis. Polyurethane foam with a density of 180 kg/m3 had a density about twice as high as that of a polyurethane foam with a density of 96 kg/m3, but yield strength was about 51% higher and cell size was about 9.5% smaller.

The role of internal architecture in producing high-strength 3D printed cobalt-chromium objects

  • Abdullah Jasim Mohammed;Ahmed Asim Al-Ali
    • The Journal of Advanced Prosthodontics
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    • v.16 no.2
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    • pp.91-104
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    • 2024
  • PURPOSE. The objectives of the current study were to estimate the influence of self-reinforced hollow structures with a graded density on the dimensional accuracy, weight, and mechanical properties of Co-Cr objects printed with the direct metal laser sintering (DMLS) technique. MATERIALS AND METHODS. Sixty-five dog-bone samples were manufactured to evaluate the dimensional accuracy of printing, weight, and tensile properties of DMLS printed Co-Cr. They were divided into Group 1 (control) (n = 5), Group 2, 3, and 4 with incorporated hollow structures based on (spherical, elliptical, and diamond) shapes; they were subdivided into subgroups (n = 5) according to the volumetric reduction (10%, 15%, 20% and 25%). Radiographic imaging and microscopic analysis of the fractographs were conducted to validate the created geometries; the dimensional accuracy, weight, yield tensile strength, and modulus of elasticity were calculated. The data were estimated by one-way ANOVA and Duncan's tests at P < .05. RESULTS. The accuracy test showed an insignificant difference in the x, y, z directions in all printed groups. The weight was significantly reduced proportionally to the reduced volume fraction. The yield strength and elastic modulus of the control group and Group 2 at 10% volume reduction were comparable and significantly higher than the other subgroups. CONCLUSION. The printing accuracy was not affected by the presence or type of the hollow geometry. The weight of Group 2 at 10% reduction was significantly lower than that of the control group. The yield strength and elastic modulus of the Group 2 at a 10% reduction showed means equivalent to the compact objects and were significantly higher than other subgroups.

A Comparative Study on the Mechanical Properties of Plywood treated with Several Fire Retardant Chemicals (I) - Effect of Soaking Time on the Static Bending Strength of Treated Plywood - (수종(數種) 내화약제(耐火藥劑)로 처리(處理)된 합판(處理)의 기술적(技術的) 성질(性質)에 관(關)한 비교연구(比較硏究)(I) - 처리합판(處理處理)의 휨강도(强度)에 미치는 침지시간(浸漬時間)의 영향(影響) -)

  • Kim, Jong-Man;Chung, Woo-Yang;Lee, Phil-Woo
    • Journal of the Korean Wood Science and Technology
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    • v.12 no.2
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    • pp.20-26
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    • 1984
  • This study was carried out to investigate the influence of chemical type and its retention in the fire-retardant treated plywoods on the static bending strength, a property peculiar to plywood. Being soaked in 20% aqueous solution of $(NH_4)_2SO_4$, $NH_4H_2PO_4$, $(NH_4)_2HPO_4$. Borax-Boric acid and Minalith for 3 to 12 hours at three-hour intervals and redried at $120^{\circ}C$ in hot press, the treated plywoods were put to static bending test. The values of chemical treated plywoods in Stress at proportional limit, Modulus of elasticity, Modulus of rupture and Work per unit volume to proportional limit were widely higher than those of water treated plywoods(control) and Borax-Boric acid treatment showed the highest value in the four mechanical data. And the bending strength of fire-retardant treated plywoods increased with the extension of soaking time or the increase of chemical retention in themselves. Borix-Boric acid was the desirable fire-retardant for thin plywood in view of mechanical strength and soaking defects in this study.

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Mechanical properties of nickel titanium and steel alloys under stress-strain test

  • GRAVINA, Marco A;QUINTAO, Catia A;KOO, Daniel;ELIAS, Carlos N
    • The korean journal of orthodontics
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    • v.33 no.6 s.101
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    • pp.465-474
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    • 2003
  • The great variety of commercial brands of orthodontic wires available on the market, stimulated by the so called superior wires (nickel titanium with shape memory effect and superelastic nickel titanium), makes the professional choice for a suitable and less expensive material difficult. The in vitro study of the mechanical properties of the orthodontic wires acts as an auxiliary tool for the professional. In this paper, a comparative study of mechanical properties was made, using stress strain tests for 4 types of orthodontic wires (conventional stainless steel, multistranded steel, superelastic nickel titanium and thermoactivated nickel titanium) separated into 5 groups. A series of 6 tests were tested for each group of wires. Initially, each group was tested 3 times until the wires broke. Furthermore, 3 more tests for each group were performed, stretching the wires under standardized activation loads, for a reliable comparison of their mechanical properties, during loading and unloading. 1 tests were applied to check differences among the groups. In vitro, the results suggest that regarding the mechanical properties supposedly desirable for physiological teeth movement, such as resilience, elasticity modulus, strength liberated during unloading, and the way that strength is liberated, thermoactivated nickel titanium wires, acting under mouth temperature, seems to be a good choice, fellowed by superelastic nickel titanium, multistranded stainless steel, and conventional stainless sleet. Superelasticity was demonstrated for superelastic nickel titanium wires. When at $37^{\circ}C$, thermoactivated nickel titanium wires showed shape memory effect, showing that temperature is important for enhancing the mechanical properties.

Changes of Physico-Chemical Properties of Beef Tenderloin Steak by Cooking Methods (조리방법에 따른 쇠고기 안심스테이크의 이화학적 변화)

  • 김천제;채영철;이의수
    • Food Science of Animal Resources
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    • v.21 no.4
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    • pp.314-322
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    • 2001
  • This study was carried out to investigate the physico-chemical properties of imported beef and Hanwo beef tenderloin steak in proximate composition, pH, myofibril Fragmentation Index (MFI), meat color, cooking loss, rheology and panel test by various cooking methods (grilling, pan-frying, oven-roasting and microwaving). moisture content of microwaving steak was lower than steak by other cooking method, and fat content of microwaving steak was higher than those of other cooking method was showed. In general, the pH values were increased with cooking, but there were no significant differences among pH values by various cooking method. The highest level of MFI in oven-roasting was showed, but no significant difference could be found among various cooking method. L* and b* -values of microwaving and pan-frying were higher than those of grilling and oven-roasting, also a* -value of microwaving was the lowest. Cooking loss was affected by cooking method. Microwaving method resulted in the highest cooking loss. Generally higher value of hardness and brittleness in microwaving than those of other cooking method were showed, and microwaving had the lowest cohesiveness, but there was no significant difference among the elasticity by the cooking method. Grilling, pan-frying and oven-roasting had high score of sensory evaluation, while microwaving had the lowest score in all sensory profile scores.

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Mechanical and Durability Properties of Partial-Depth Patch Materials using Polymer Materials for Concrete Pavement (단면보수용 콘크리트 패치재료의 역학적 특성 및 내구성 실험)

  • Yang, Sung-Chul;Hwang, In-Dong;Han, Seong-Hwan;Kim, Seong-Min
    • International Journal of Highway Engineering
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    • v.11 no.3
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    • pp.23-32
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    • 2009
  • In this paper an experimental program was launched to determine the mechanical and durability properties of spall repair materials (RCC: 3 items, PCC: 2 items, PC: 3 items). Test items were mechanical property tests such as setting times, strengths, modulus of elasticity, plastic shrinkage, and durability tests such as dynamic modulus ratio, bond property with freeze-thaw, water absorption, chemical resistance, ultraviolet exposure. Modulus of the PC products exhibits ductile while the modulus is in the order of RCC > PCC > PC. At early ages the PC products experience higher plastic shrinkage than others, henceforth stable at 28 days. Other test results such as dynamic modulus ratio, absorption, and chemical resistance show that the PCs are superior to the PCCs and the RCCs. Except for PC-2, all patch materials had bond strength more than 1.3MPa after freeze-thaw cycles of 200~300 while the PCs and the PCCs seem to be better than the RCCs. With 500 hours of ultraviolet exposure, all patch materials showed to have no crack or deterioration at the surface.

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Feasibility of Domestic Yellow Poplar (Liriodendron tulipifera) Dimension Lumber for Structural Uses (국산 백합나무 구조용 제재목의 이용가능성 평가)

  • Lim, Jin-Ah;Oh, Jung-Kwon;Yeo, Hwan-Myeong;Lee, Jun-Jae
    • Journal of the Korean Wood Science and Technology
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    • v.38 no.6
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    • pp.470-479
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    • 2010
  • In this study, the visual grading based on the visual characteristics and structural timber bending test were conducted for domestic yellow poplar dimension lumber. Structural performance of domestic yellow poplar dimension lumber was conducted through the evaluation of strength and stiffness. Visual grading rule of yellow poplar dimension lumber did not exist in Korea. Visual grading of yellow poplar dimension lumber was performed according to the NSLB (Northern Softwood Lumber Bureau) standard grading rules including several hardwood dimension lumber. The allowable bending stress was calculated from the results of a visual grading. Compared with NDS (National Design Specification), the yellow poplar dimension lumber showed enough strength for structural uses. In addition, the visual grading was performed according to the KFRI (Korea Forest Research Institute) grading rule to calculated allowable bending stress and to evaluated the feasibility. The yellow poplar was classified into the pine groups by the KFRI criteria regulated by specific gravity. Allowable bending stress based on weibull distribution had became highly than KFRI criteria, as No. 1 (10.0 MPa), No. 2 (7.4 MPa) and No. 3 (4.1 MPa). And the availability of yellow poplar dimension lumber for structural uses had been confirmed. The Modulus of Elasticity (MOE) of domestic yellow poplar dimension lumber had not met the NDS and KFRI criteria. However, for the use of domestic yellow poplar, average values of MOE which obtained through this test were suggested as design value for domestic yellow poplar. Design values were supposed No. 1, 2 (9,000 MPa) and No. 3 (8,000 MPa).

Study on the Tensile Strength of Oxidative Permanent Dyed Hair (산화형 영구 염모제로 처리한 모발의 인장강도 특성에 관한 연구)

  • Lee, Gui-Young;Chang, Byung-Soo
    • Applied Microscopy
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    • v.38 no.4
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    • pp.339-345
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    • 2008
  • In this study, we compared the stress and tensile strength of the hair treated with oxidative permanent dye with those of virgin hair. We investigated the fine structure of the hair section after the tensile test using scanning electron microscopy. The tensile strength of the virgin hair was measured as $14.66\;g/cm^2$, tensile energy was $108\;erg/cm^2$, and the maximum stress was 146.64g. Those of the dyed hair were $13.69\;g/cm^2$, $89.62\;erg/cm^2$ and 136.90 g, respectively. The differences in the tensile strength, the tensile energy and the maximum stress were $-0.97\;g/cm^2$, $-18.38\;erg/cm^2$, -9.74 g, respectively, which showed that the dyed hair had less elasticity and strength than the virgin hair. In the scanning electron microscopy investigation of the damaged hair after the tensile test, lift-off of the cuticle outer layer were shown in both virgin hair and dyed hair, which was more severe in the dyed hair than the virgin hair. Adjacent cuticular cells of the cuticle layer were separated by the destruction of intercellular membrane complex. The macrofibrils were exposed and separated from the cortex torn by tensile strength.

Effects of Adding Sourdough Starter Powder using Korean Wheat Flour on the Quality of Pan Bread (국내산 밀가루를 이용한 사워종 분말의 첨가가 식빵의 품질에 미치는 영향)

  • An, Hye-Lyung;Lee, Kwang-Suck
    • Culinary science and hospitality research
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    • v.18 no.4
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    • pp.183-198
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    • 2012
  • The purpose of this study is to determine potential effectiveness of sourdough starter powder (made of Korean wheat flour) in the quality characteristics of bread and in the substitution effect of a dough improver in bread baking. The bread containing sourdough starter powder was considered to be most suitable for making sourdough bread. As a result, SP 10 showed the best fermentation rate and the highest volume and specific volume. In sensory testing, SP 10 was most preferable among others. According to experimental comparison between optimal addition ratio of SP 10 and other sourdough starter powder products available in Korean domestic market, SI had the highest fermentation rate, highest volume and specific volume. Analysis of texture showed that SI was soft with the lowest hardness and the highest elasticity. Sensory test showed that SI was most preferable of all, and SP 10 was more preferable than BP. It was concluded that SP 10 was not less preferable in the aspect of preference at sensory test than other products. Hence, it is expected that sourdough starter powder made of Korean wheat flour will be possibly more popular and effective in replacing some portion of dough improver of baking.

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