• Journal of the Korean Institute of Educational Facilities
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• v.26 no.5
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• pp.3-10
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• 2019

This study has a purpose to profile local sectors into meaningful groups by using facilities rates of Social Overhead Capital(SOC) for daily life. Comparing SOC for daily life among the meaningful groups, the profiling and comparison results bring the comprehensive understanding about the educational facilities in local sectors. For the research purpose, this study utilized Latent Profile Analysis(LPA) by using variables such as population, road information, SOC for daily life, usage of land, possession of land, and appraised value of land from the 2018 Geographic Information System(GIS) dataset of Gwangjin-gu, where is one of the administrative district of Seoul City. Results showed that there are four latent groups of sectors among 904 local sectors(100 squared-meters sector per each) in Gwangjin-gu. By comparing the four latent groups by using LPA, the results diagnose each sector's status and help to improve the policy about educational facilities. Specifically, by using dataset for SOC of daily life, there are four groups of local sectors and each group has different features. Based on the different features of local sector groups, there can be improved management of educational facilities matching with each group's features.

• Journal of Microbiology and Biotechnology
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• v.28 no.7
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• pp.1185-1198
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• 2018

The nucleosomal organization of chromatin using histone proteins is a fundamental and ubiquitous feature of eukaryotic nuclei, with the major exception of dinoflagellates. Although a number of recent genomic and transcriptomic analyses have detected numerous histone genes in dinoflagellates, little is known about their expression. Here in, we aimed to investigate the expression pattern of histone genes under nutritional stress, and an attempt was made to detect histone expression at the protein level in Alexandrium pacificum. The presence of histones at the mRNA level was confirmed in this study by the amplification, cloning, and sequencing of 10 different genes. Relative expression profiling of these genes under different growth conditions was determined with real-time PCR and revealed considerable levels of histone transcription in nutritionally stressed cells. We were unable to detect the expression of histones at the protein level even after immunodetection and analysis using mass spectrometry, although a histone-like protein was detected as a major nuclear component. A. pacificum expresses multiple variants of histone, and protein sequences revealed both conservation and divergence with respect to other eukaryotes. We concluded that A. pacificum maintained an active transcription of histone genes within the cell, and enhanced expression of histone genes in nutritional stress strongly suggest that histones have functional significance in dinoflagellates, although expression at the protein level was below our current detection limits, which suggests a limited role of histones in DNA packaging. Finally, the plausible regulation of histone expression at the gene and protein levels in A. pacificum is discussed.

• BMB Reports
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• v.38 no.2
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• pp.151-160
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• 2005

By a cDNA array representing 2308 signal transduction related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RT-PCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeletion and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.

• Journal of Fisheries and Marine Sciences Education
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• v.25 no.4
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• pp.958-972
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• 2013

The purpose of this study is to analyze on high school teachers' stage of concern(SoC) on 'Creative-Experience Activity' in 2009 revised national curriculum and investigate the improvements of their levels. The subjects for this study were 234 high school teachers through out all part of the Korea country. The instrument for this study was developed according to the Hall & Hord(2006)'s stage of concerns questionnaire of Stage of Concern. The Data were analyzed by Profiling of teachers' concern and one-way ANOVA. The major findings of this study were as follows: First, 85.25% of the high school teachers were in stage of Awareness. 6.34% of teachers were in stage of Information. Most of high school teachers' concern about 'Creative-Experience Activity' was generally 'non-user' stage that unconcerned. Second, there were not significant differences in teachers' stage of concern according to their sex and teaching career except for region. In region, the teacher in metropolis tent to interest getting new information than small and midium size city. We need to make an effort to transfer from 'non-user' stage to 'early-user' stage of and 'impact' stage. To do so, we may sublate too much curriculum revise and start teacher training for 2009 revised 'creativity-experience activity' curriculum. The result of the study provide that there are not curriculum implementation success without teachers' concern about revised curriculum.

• The Mathematical Education
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• v.57 no.2
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• pp.75-92
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• 2018

Based on Perceptions about Mathematics Teachers (PMT) perceived by high school students, measured by 2189 students from Seoul Educational Longitudinal Study 2014 (SELS 2014), latent profile analysis (LPA) identified five distinct types of student groups (positive, partial positive, middle, negative, extreme negative). These student of positive, middle, and negative groups are positive, moderate and negative perceptions about math teachers. Partial positive group generally had a positive perception about mathematics teachers, extremely negative group was very negative about mathematics teachers. Both of these groups had peculiarly inconsistent trends and several anomalies. The Multinomial logistic regression analyses also indicated that individual factors (gender, major, self-concept, resilience, self-assessment, career maturity), school factors (friendship, relationship with school teachers) and parental factors (academic-relationship, emotional-relationship) were significant predictors of PMT profile groups. The Analysis of variance also indicated that mathematics class (attitude, satisfaction and atmosphere), Mathematics achievement were significant predictors of PMT profile groups. The profiling of perceptions about mathematics teachers resulted in enhanced understanding of the complex range of processes students employed. During mathematics class, implementation of smooth interactions and communications between students and teachers added in the teaching and learning of mathematics.

• Journal of Microbiology and Biotechnology
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• v.26 no.11
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• pp.1993-2005
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• 2016

Bacillus pumilus is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. A novel B. pumilus SCU11 strain possessing high alkaline protease activity was obtained in our previous work. The culture supernatant of this strain showed efficient dehairing capability with minimal collagen damage, indicating promising potential applications in the leather industry. In this study, the strain's extracellular proteome was identified by LC-MS/MS-based shotgun proteomic analysis, and their related secretory pathways were characterized by BLAST searches. A total of 513 proteins, including 100 actual secreted and 413 intracellular proteins, were detected in the extracellular proteome. The functions of these secreted proteins were elucidated and four complete secretory systems (Sec, Tat, Com, and ABC transporter) were proposed for B. pumilus. These data provide B. pumilus a comprehensive extracellular proteome profile, which is a valuable theoretical and applicative basis for future genetic modifications and development of industrial enzymes.

• Steel and Composite Structures
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• v.30 no.5
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• pp.405-416
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• 2019

Profiled composite slab has been widely used in civil engineering due to its structural merits. The extension of this concept to the bearing wall forms the profiled composite wall, which consists of two external profiled steel plates and infill concrete. This paper investigates the structural behavior of this type of wall under axial compression. A series of compression tests on profiled composite walls consisting of varied types of profiled steel plate and edge confinement have been carried out. The test results are evaluated in terms of failure modes, load-axial displacement curves, strength index, ductility ratio, and load-strain response. It is found that the type of profiled steel plate has influence on the axial capacity and strength index, while edge confinement affects the failure mode and ductility. The test data are compared with the predictions by modern codes such as AISC 360, BS EN 1994-1-1, and CECS 159. It shows that BS EN 1994-1-1 and CECS 159 significantly overestimate the actual compressive capacity of profiled composite walls, while AISC 360 offers reasonable predictions. A method is then proposed, which takes into account the local buckling of profiled steel plates and the reduction in the concrete resistance due to profiling. The predictions show good correlation with the test results.

• Journal of Apiculture
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• v.32 no.3
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• pp.191-198
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• 2017

Vespa hornets are notorious predators of honeybees in Korean beekeeping. Detection of vespa hornet attacking on honeybee colony was tried through analysis of wing beat frequency profiling from Vespa mandarinia. Wing beat profiles of V. mandarinia during active flight and resting were distinctively different. From the wing beat profiling, algorithm of automated detection of vespa attack was encoded, and alert system was developed using Teensy 3.2 and Raspberry pi 3. From the laboratory testing, the prototype system successfully detected vespa wing beats and delivered the vespa attack information to the user wirelessly. Further development of the system could help precision alert system of the vespa attack to apiary.

• BMB Reports
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• v.39 no.5
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• pp.595-606
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• 2006

Metallothioneins are a group of low molecular mass and cysteine-rich metal-binding proteins, ubiquitously found in most living organisms. They play an important role in maintaining intracellular metal homeostasis, eliminating metal toxification and protecting against intracellular oxidative damages. Analysis of complete rice genome sequences revealed eleven genes encoding putative metallothionein (OsMT), indicating that OsMTs constitute a small gene family in rice. Expression profiling revealed that each member of the OsMT gene family differs not only in sequence but also in their tissue expression patterns, suggesting that these isoforms may have different functions they perform in specific tissues. On the basis of OsMT structural and phylogenetic analysis, the OsMT family was classified as two classes and class I was subdivided into four types. Additionally, in this paper we also present a complete overview of this family, describing the gene structure, genome localization, upstream regulatory element, and exon/intron organization of each member in order to provide valuable insight into this OsMT gene family.

• Journal of Fisheries and Marine Sciences Education
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• v.22 no.2
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• pp.287-302
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• 2010

The purpose of the study was to figure out elementary school teachers' Stages of Concern(SoC) between senior teachers and new comers to integrated units of integrated curriculum which was revised in 2007 and applied in 2009 by using CBAM. Seventy-four home-room teachers in elementary schools took part as participants. For data collection, as Hall and Hord suggested in 2005, Questionnaire of Stages of Concern was used. Profiling of teachers' concern and one-way ANOVA were employed in data analysis. In the results, 55% of the teachers were in the stage of Awareness. In the stage of Informational, 22% of the teachers were appeared. Most of elementary teachers' concern about integrated units was closed to indifference of self-interest level. The difference of concern between senior teachers and others will be illustrated as findings. The results of the study provided that factors which influenced teachers' concern about the curriculum. Physical factors such as a curriculum guideline or on-line system to access boarder-cross curriculum influenced teachers' concern. Little intervention about the boarder-cross curriculum appeared in elementary schools. The authors of the study suggested to emphasizing the roles of facilitator for the revised curriculum.