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Gene Expression Profiles of HeLa Cells Impacted by Hepatitis C Virus Non-structural Protein NS4B

  • Zheng, Yi (Key Laboratory of Virology, Ministry of Education, College of Life Sciences, Wuhan University) ;
  • Ye, Lin-Bai (Key Laboratory of Virology, Ministry of Education, College of Life Sciences, Wuhan University) ;
  • Liu, Jing (Key Laboratory of Virology, Ministry of Education, College of Life Sciences, Wuhan University) ;
  • Jing, Wei (Key Laboratory of Virology, Ministry of Education, College of Life Sciences, Wuhan University) ;
  • Timani, Khalid A. (Key Laboratory of Virology, Ministry of Education, College of Life Sciences, Wuhan University) ;
  • Yang, Xiao-Jun (Key Laboratory of Virology, Ministry of Education, College of Life Sciences, Wuhan University) ;
  • Yang, Fan (Key Laboratory of Virology, Ministry of Education, College of Life Sciences, Wuhan University) ;
  • Wang, Wei (Key Laboratory of Virology, Ministry of Education, College of Life Sciences, Wuhan University) ;
  • Gao, Bo (Key Laboratory of Virology, Ministry of Education, College of Life Sciences, Wuhan University) ;
  • Wu, Zhen-Hui (Key Laboratory of Virology, Ministry of Education, College of Life Sciences, Wuhan University)
  • Published : 2005.03.31
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Abstract

By a cDNA array representing 2308 signal transduction related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RT-PCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transiently transfected HeLa cells and Huh-7, a human hepatoma cell line. Of the 2,308 genes we examined, 34 were up-regulated and 56 were down-regulated. These 90 genes involved oncogenes, tumor suppressors, cell receptors, complements, adhesions, transcription and translation, cytoskeletion and cellular stress. The expression profiling suggested that multiple regulatory pathways were affected by HCV-NS4B directly or indirectly. And since these genes are related to carcinogenesis, host defense system and cell homeostatic mechanism, we can conclude that HCV-NS4B could play some important roles in the pathogenesis mechanism of HCV.

Keywords

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