• Journal of Life Science
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• v.8 no.2
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• pp.203-207
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• 1998

NAD4 as a gene encoding NADH dehydrogenase subunit 4 in the micodhondrion from maize has been cloned using RT-PCR and sequenced for examining RNA edited sites. Analysis of mt cDNA sequences showed the typical RNA editing patterns and unusual base changes as well;RNA editing from cDNA sequences occured base change from c to U in most cases, however transitions from t to g and G to A were also observed. Even though those editings appared to be occurred randomly, RNA edited sites showed mostly in exon 1 and exon 4 regions, when compared with NAD4 cDNA from wheat, locations of edited sites did not consistent with each other suggesting that the phenomenon of RNA editing occured randomly not site-specific manner.

• BMB Reports
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• v.54 no.2
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• pp.98-105
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• 2021

The clustered regularly interspaced short palindromic repeats (CRISPR) system is a family of DNA sequences originally discovered as a type of acquired immunity in prokaryotes such as bacteria and archaea. In many CRISPR systems, the functional ribonucleoproteins (RNPs) are composed of CRISPR protein and guide RNAs. They selectively bind and cleave specific target DNAs or RNAs, based on sequences complementary to the guide RNA. The specific targeted cleavage of the nucleic acids by CRISPR has been broadly utilized in genome editing methods. In the process of genome editing of eukaryotic cells, CRISPR-mediated DNA double-strand breaks (DSB) at specific genomic loci activate the endogenous DNA repair systems and induce mutations at the target sites with high efficiencies. Two of the major endogenous DNA repair machineries are non-homologous end joining (NHEJ) and homology-directed repair (HDR). In case of DSB, the two repair pathways operate in competition, resulting in several possible outcomes including deletions, insertions, and substitutions. Due to the inherent stochasticity of DSB-based genome editing methods, it was difficult to achieve defined single-base changes without unanticipated random mutation patterns. In order to overcome the heterogeneity in DSB-mediated genome editing, novel methods have been developed to incorporate precise single-base level changes without inducing DSB. The approaches utilized catalytically compromised CRISPR in conjunction with base-modifying enzymes and DNA polymerases, to accomplish highly efficient and precise genome editing of single and multiple bases. In this review, we introduce some of the advances in single-base level CRISPR genome editing methods and their applications.

• Journal of Digital Contents Society
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• v.18 no.8
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• pp.1621-1626
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• 2017

This study conducts data visualization of common climax patterns of Korean blockbuster films to analyze shots and evaluate scene (subplot unit) arrangement. For this purpose, a model of editing patterns is used to analyze how many climax patterns a film contains. Moreover, a system, which automatically collects shot images and classifies shot sizes of collected data, is designed to demonstrate that a single scene is composed based on a climax pattern. As a scene is a subplot and thus its arrangement cannot fully be analyzed only by climax patterns, dialogues of starring actors are also used to identify scenes, and the result is compared with data visualization results. It detects dialogues between particular actors and visualizes dialogue formation in a network form. Such network visualization enables the arrangement of main subplots to be analyzed, and the box office performance of a film can be explained by the density of subplots. The study of two types comparison analysis is expected to contribute to planning, plotting, and producing films.

• Journal of Broadcast Engineering
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• v.25 no.6
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• pp.861-869
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• 2020

We introduce the application that automatically makes several images stored in user's device into one video by using the different climax patterns appearing for each film genre. For the classification of the genre characteristics of movies, a climax pattern model style was created by analyzing the genre of domestic movie drama, action, horror and foreign movie drama, action, and horror. The climax pattern was characterized by the change in shot size, the length of the shot, and the frequency of insert use in a specific scene part of the movie, and the result was visualized. The model visualized by genre developed as a template using Firebase DB. Images stored in the user's device were selected and matched with the climax pattern model developed as a template for each genre. Although it is a short video, it is a feature of the proposed application that it can create an emotional story video that reflects the characteristics of the genre. Recently, platform operators such as YouTube and Naver are upgrading applications that automatically generate video using a picture or video taken by the user directly with a smartphone. However, applications that have genre characteristics like movies or include video-generation technology to show stories are still insufficient. It is predicted that the proposed automatic video editing has the potential to develop into a video editing application capable of transmitting emotions.

• 한국HCI학회:학술대회논문집
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• 2008.02a
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• pp.256-261
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• 2008

We developed an authoring tool for designing vibrotactile patterns quickly and easily by using the drag-and-drop paradigm in mobile devices. Designed vibrotactile patterns are registered into a data pool in the XML format, improving the reusability and extensibility of vibrotactile patterns. A multi-channel timeline interface is also incorporated to provide time-synchronized pattern editing for multiple vibration patterns (for multiple vibration actuators). In addition, an internal vibration player is embedded in the authoring tool in order to evaluate the patterns on the spot. A transform function for perceptually transparent vibration rendering can also be set in the editor. Although the authoring tool was developed for mobile devices, it can be used for other applications such as haptic interaction m virtual reality.

• Journal of the Korean Society of Clothing and Textiles
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• v.39 no.3
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• pp.457-467
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• 2015

The demand for coverall-type protective clothing are increasing in many industrial fields; however, it is impossible to evaluate the wearers' apparel fit because there is no commercialized pattern in the market. This study produces a manual for the process of acquiring patterns by separating PPE products into pieces to provide information on acquiring patterns. The analysis was on coverall-type non woven protective clothing in the domestic market, and 4 panels examined the validity, reliability, and efficiency of various possible methods on each step of process. Five steps were conducted to acquire patterns removing wrinkles, marking seams lines, separating seams, and pattern completing. The process of converting these into pattern files was designed through three procedures of digitizing, editing, and exporting and detailed stages. Fitting tests were undertaken, real patterns by actual modeling and pattern files by virtual modeling; all showed similar forms with outfits wearing ready-made protective coveralls. This study sought the most efficient and objective method of virtual modeling and proposed it as a manual. It is expected for the pattern reverse-designing manual through products analysis presented in this study that would be a helpful addition of information to the pattern tracing of pattern-less clothing products.

• BMB Reports
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• v.57 no.2
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• pp.86-91
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• 2024

The fatty acids content of castor (Ricinus communis L.) seed oil is 80-90% ricinoleic acid, which is a hydroxy fatty acid (HFA). The structures and functional groups of HFAs are different from those of common fatty acids and are useful for various industrial applications. However, castor seeds contain the toxin ricin and an allergenic protein, which limit their cultivation. Accordingly, many researchers are conducting studies to enhance the production of HFAs in Arabidopsis thaliana, a model plant for oil crops. Oleate 12-hydroxylase from castor (RcFAH12), which synthesizes HFA (18:1-OH), was transformed into an Arabidopsis fae1 mutant, resulting in the CL37 line producing a maximum of 17% HFA content. In addition, castor phospholipid:diacylglycerol acyltransferase 1-2 (RcPDAT1-2), which catalyzes the production of triacylglycerol by transferring HFA from phosphatidylcholine to diacylglycerol, was transformed into the CL37 line to develop a P327 line that produces 25% HFA. In this study, we investigated changes in HFA content when endogenous Arabidopsis PDAT1 (AtPDAT1) of the P327 line was edited using the CRISPR/Cas9 technique. The successful mutation resulted in three independent lines with different mutation patterns, which were transmitted until the T4 generation. Fatty acid analysis of the seeds showed that HFA content decreased in all three mutant lines. These findings indicate that AtPDAT1 as well as RcPDAT1-2 in the P327 line are involved in transferring and increasing HFAs to triacylglycerol.

• Journal of Korea Multimedia Society
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• v.17 no.11
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• pp.1263-1269
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• 2014

As rapid development of new hardware control interface technology, new concepts have been being proposed and emerged. In this paper, a new approach based on leap motion system is proposed. While we employ a position information from sensor, the hand gesture recognition is suggested with the pre-defined patterns. To do this, we design a recognition algorithm with hand gesture and finger patterns. We apply the proposed scheme to 3-dimensional avatar controling and editing software tool for making animation in the cyber space as a representative application. This proposed algorithm can be used to control computer systems in medical treatment, game, education and other various areas.

• Journal of the Korean Home Economics Association
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• v.44 no.2 s.216
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• pp.127-135
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• 2006

This paper suggests guidelines for virtual clothes modeling and draping software suitable for clothes designers. We first analyze Maya Cloth, which is widely used in game and animation fields, and which has been adopted by Pad System as a 3D cloth draping system. We then discuss what functions and procedures would improve Maya Cloth to assist designers in being able to create the clothes they have conceptualized. While Maya Cloth has many good functions and features forvirtual cloth modeling and draping, it treats input 2D patterns as approximat and it creates 3D clothes by considering other factors such as the 3D body model. As a result, it is hard for clothes designers to control the shape of the 3D clothes by changing 2D patterns. Furthermore, Maya Cloth does not handle seamlines satisfactorily. We suggest that the following new features should be added to Maya Cloth : respecting the input 2D patterns, handling seamlines, and controlling the shape of the clothes in 3D space.

• Journal of Internet Computing and Services
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• v.5 no.1
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• pp.1-13
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• 2004

The effect of Application system with class units is not sufficient because of independency and reuse of Component elements due to component abstraction based on only source code. Therefore We need to apply design pattern approach to represent not only the problem abstraction but also information and relationship between system elements for generic solutions of specific domain, Also, it is essential to software reverse engineering acquiring the correct understandings of the system through examining the existing systems and utilizing the acquired knowledges as reusable resources. In this paper, the extraction algorithm with JAVA and the validity of applying reverse engineering with extracting design patterns from source codes of the existing object-oriented system; are devised. The architecture of automatic tool is designed and implemented for 1) automatic extraction of design patterns and 2) reuse tool for retrieving, editing and rebuilding of design patterns.