Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지

Molecular cDNA cloning and unusual RNA editings of NAD4 gene from Zea mays mitochondrion

옥수수 미토콘드리아 NAD4유전자의 cDNA cloning과 특이한 RNA editing 현상

  • 설일환 (경북대학교 농업과학 기술연구소)
  • Published : 1998.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

NAD4 as a gene encoding NADH dehydrogenase subunit 4 in the micodhondrion from maize has been cloned using RT-PCR and sequenced for examining RNA edited sites. Analysis of mt cDNA sequences showed the typical RNA editing patterns and unusual base changes as well;RNA editing from cDNA sequences occured base change from c to U in most cases, however transitions from t to g and G to A were also observed. Even though those editings appared to be occurred randomly, RNA edited sites showed mostly in exon 1 and exon 4 regions, when compared with NAD4 cDNA from wheat, locations of edited sites did not consistent with each other suggesting that the phenomenon of RNA editing occured randomly not site-specific manner.

본 연구는 옥수수에서 분리한 미토콘드리아에서 NADH-dehydrogenase 유전자 (subunit 4)의 cDNA를 RT-PCR의 방법을 사용하여 조제 한 ㅜ 염기서열 수행한 경과 특이한 점을 감지 할 수 있었다. 일반적인 RNA cditing은 C에서 U로 또는 U에서 C로 치환되는 현장으로 옥수수의 NAD4유전자에서도 이러한 editing 형상이 일어나는 것을 발견하였다. 또는 T가 G로 그리고 G 가 A로 변화되는 특이한 부분들이 생성되는 것을 관찰하였다. 이러한 RNA ediring은 주로 exon 1과 exon 4 에 많이 일어나며, 염기 치환되는 부분들은 에서늬 NAD4유전자의 RNA edting site들과 일피하지 않은 점으로 미루어 보아 RNA editing 현상은 무작의로 생성된다고 본다.된다고 본다.

Keywords

References

  1. Plant Cell v.2 RNA editing of wheat mitochondrial ATP synthase subunit 9 : direct protwin and cDNA sequencing Begu, D.;Graves, P. V.;Domec, C.;Arselin, G.;Livak, S.;Araya, A.
  2. Cell v.65 The wheat mitochondrial fene for subunit-Ⅰ of the NADH dehydrogenase complex - A trans splicing model for this gene-in-pieces Chapdelain, Y.;Bonen, L.
  3. J. Mol. Biol. v.214 Direct protein sequencing of wheat mitochondrial ATP synthase subunit 9 confirms RNA deiting in plants Graves, P. V.;Begu, D.;Velours, J.;Neau, E.;Belloc, F.;Litvak, S.;Araya, A.
  4. Nucl. Acids Res. v.19 RNA editing of the transcript coding for subunit-4 of NADH dehydrogenase in wheat mitochondria - Uneven distribution of the editing sites aming the 4 exons Lamatina, L.;Grienenberger, J. M.
  5. Plant Mol. Biol. v.32 RNA editing in plant mitochondria and chloroplaste Maier, R. M.;Zeltz, P.;Kossel, H.;Bonnard, G.;Gualberto, J. M.;Grienenberger, J. M.
  6. Plant physiol. v.104 The nad4 gene of maize mitochondria is highly conserved Marinefeld, J. R.;Newton, K. J.
  7. Mol. Cell. Biol. v.10 RNA editing in the cytochrome b locus of the higher plant Oenothera berteriana includes a U-to-C transition. Schuster, W.;Hiesel, R.;Wissinger, B.;Brennicke, A.
  8. Methods Enzymol v.118 Isolation of plant mitochondrial RNA Stern, D. B.;Newton, K. J.
  9. Plant physiology. Taiz, L.;Zeiger, E.