• 한국발생생물학회지:발생과생식
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• 제16권4호
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• pp.301-307
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• 2012

Olive flounder Paralichthys olivaceus is one of the most widely cultured fish species in Korea. Although olive flounder receive attention from aquaculture and fisheries and extensive research has been conducted eye morphological change in metamorphosis, but little information was known to molecular mechanism and gene expression of eye development- related genes during the early part of eye formation period. For the reason of eyesight is the most important sense in flounder larvae to search prey, the screening and identification of expressed genes in the eye will provide useful insight into the molecular regulation mechanism of eye development in olive flounder. Through the search of an olive flounder DNA database of expressed sequence tags (EST), we found a partial sequence that was similar to crystallin beta A1 and gamma S. Microscopic observation of retinal formation correspond with the time of expression of the crystallin beta A1 and gamma S gene in the developmental stage, these result suggesting that beta A1 and gamma S play a vital role in the remodeling of the retina during eye development. The expression of crystallin beta A1 and gamma S were obviously strong in eye at all tested developing stage, it is also hypothesized that crystallin acts as a molecular chaperone to prevent protein aggregation during maturation and aging in the eye.

• 아시안잔디학회지
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• 제13권2호
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• pp.101-112
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• 1999

Four major scarab beetles, Ectinohoplia rufipes, Adoretus tenuimaculatus, Exomala orientalis and Popillia quadriguttata were obserbved in golf courses. Morphological characters of adults and larvae, feeding habit, mounds by grubs, and outbreak time were inbestigated. Body lengths of E. rufipes and A. tenuimaculatus were 11.5mm and 9.7-11.3mm, respectively and those of E. orientalis and P. quadriguttata were 8.0-13.5mm and 7.6-12.7mm, respectively. Adult of E. rufipes had a tendency to raise hind legs during the feeding and mating but showed no death feigning. On the contrary, A. tenuimaculatus adult did not raise its hind legs during the feding and showed death feigning and dropped to earth when disturbed. E. orientalis adults were mainly found from the flowers of chestnut trees in the daytime and P. quadriguttata adults were chiefly found from soft leaves of herbaceous plants in golf courses. White grubs of E. rufipes and A. tenuimaculatus did not have longitudinal and transversal seatae on anal part but setae on the raster were hooked. The shape of anal slit was Y-shape in E. rufipes and crescent-shape in A. tenuimaculatus. White grubs of E. orientalis had ten to sixteen longitudinal setae on raster and those of P. quadriguttata had medially two conspicuous rows of six or seven shorter straight setae forming a V. Adults of E. rufipes mainly fed on front part of leaves but those of A. tenuimaculatus fed on hind part of leaves. Mounds by earthworms and white grubs were different depending on species. Earthworm piled up sticky soil around exit hole but E. orientalis adult scattered soil one part of exit hole and A. tenuimaculatus adult scattered soil evenly around exit hole. Outbreak time of E. rufipes and A. tenuimaculatus was early May and that of E. orientalis and P. quadriguttata was late May.

• Weed & Turfgrass Science
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• 제6권4호
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• pp.355-358
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• 2017

Amur silver grass, Miscanthus sacchariflorus is one of the promising biofuel weeds. A damage of noctuid pest, Leucapamea askoldis was firstly observed from Amur silver grass in Hwasun silver grass plantation during the survey of insect pests of Amur silver grass in Iksan, Hwasun, and Sancheong plantation areas in Korea. The host of L. askoldis was not known yet in Korea. The L. askoldis damage was observed as larval feeding on newly grown shoots and roots of M. sacchariflorus close to soil surface from early May in 2013. Investigated larval density was $1.6{\pm}1.1per\;m^2$ on April 4 and damage rate of shoots was $0.8{\pm}0.4%$ on May 4, 2013. The larvae bore into shoots of M. sacchariflorus and feed on the inside of plant. The damaged shoots are easily pulled out and distinguished by the boring hole on the shoots. L. askoldis was potential insect pest in M. sacchariflorus plantation areas.

• International Journal of Industrial Entomology and Biomaterials
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• 제10권1호
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• pp.11-17
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• 2005

In our research to identify gene involved in the cuticle protein, we cloned a novel cuticle protein gene, ApCP15.5, from the Chinese oak silkmoth, Antheraea pernyi, larvae cDNA library. The gene encodes a 149 amino acid polypeptide with a predicted molecular mass of 15.5 kDa and a pI of 9.54. The ApCP15.5 contained a type-specific consensus sequence identifiable in other insect cuticle proteins and the deduced amino acid sequence of the ApCP15.5 cDNA is most homologous to Tenebrio molitor-C1B ($43\%$ protein sequence identity), followed by Locusta migratoria-76 ($42\%$ protein sequence identity). Northern blot and Western blot analyses revealed that the ApCP15.5 showed the epidermis-specific expression. The expression profile of ApCP15.5 indicated that the ApCP15.5 mRNA expression was detected in the early stages after larval ecdysis and larval-pupal metamorphosis, and its expression level was most significant on the first day of larval ecdysis and pupal stage. The ApCP15.5 was expressed as a 15.5 kDa polypeptide in baculovirus-infected insect cells.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권1호
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• pp.15-20
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• 2003

The present investigation was carried out to study the possible cause for reciprocal difference in silkworm hybrids. By utilising the polyvoltine race Pure Mysore (PM) and newly evolved breeds (CSR2, CSR5, CSR16 and CSR17), the direct and reciprocal crosses of polyvoltine ${\times}$ bivoltine and also bivoltine hybrids were studied. The hybrids of polyvoltine ${\times}$ bivoltine (direct) are superior to their reciprocal crosses in respect of cocoon yield, cocoon weight and filament length. The reciprocal crosses of polyvoltine ${\times}$ bivoltine are superior to their direct crosses in respect of fecundity and short larval duration. No significant differences were observed in the characters like cocoon shell ratio, raw silk percentage, denier, reelability and neatness in both polyvoltine ${\times}$ bivoltine direct crosses and their reciprocals. The expression of cocoon characters as a function of sex revealed that direct crosses (polyvoltine ${\times}$ bivoltine) showed higher cocoon weight, pupal weight, shell weight and longer filament length in females than the reciprocal crosses (bivoltine ${\times}$ polyvoltine), where as these characters in males were almost the same in both direct and reciprocal crosses, indicating that the sex-linked genetic factor played a more important role. it was clear that difference in cocoon yield observed in reciprocal crosses of polyvoltine ${\times}$ bivoltine was due to the low cocoon and shell weight in females which was turn due to presence of early maturity genes (Lme) linked with sex-chromosome (X) which effect on larvae period of the silkworm. In bivoltine hybrids, i.e., both direct and their reciprocals crosses, all the characters viz., hatching percentage, larval duration, survival, cocoon weight, cocoon shell weight, cocoon shell ratio, raw silk percentage, filament length, denier, reelability and neatness did not show any significant difference (except number of eggs laid by moth) which could account for presence of same maturity genes (Lm) in both direct and reciprocal crosses. it was clear that reciprocal differences occur when the hybrids are prepared from the parental strains with different voltinism.

• Fisheries and Aquatic Sciences
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• 제8권1호
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• pp.27-33
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• 2005

This is a subsequent study to our previous finding that Pacific oysters, Crassostrea gigas, gained a so-called upper plateau concentration, around 30,000 ng/g dry weight digestive gland for benzo(a)pyrene, showed reproductive behavior but produced their ensuing reproductive outputs damaged. A serial dilution of sediment elutriates from Jinhae Bay, Korea, where pollution was progressive, were exposed to gametes of the Pacific oyster for 0, 5, 10, 20, 30, and 60 min to detail the pollutant effects on very young specimens. There was an apparent critical dilution over which adverse effects are evident. This was $10\%$ of the present sediment elutriate, corresponding to 0.3 ng/g on the basis of total polycyclic aromatic hydrocarbons (PAHs) for the oyster. Within the dilution the embryonic development was not influenced by the duration of exposure to its gamete stage. At higher dilutions over the critical dilution, occurrence of abnormality increased dependent on the pollutant dilution and the duration of exposure. Similar trends were also found in larval mortality. However, overall, the chemical toxicity was more significant to morphogenesis than to survival, suggesting a potential recruitment of the pollutants-induced abnormal larvae in the wild population to threaten the population integrity.

• 한국어류학회지
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• 제8권1호
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• pp.9-13
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• 1996

1995년 4월부터 12월까지 군산연안 천해역에 서식하는 감성돔 Acanthopagrus schlegeli(Bleeker)의 성장과 서식환경에 대해 조사하였다. 감성돔의 부유성 치어는 6월 초순에 출현하였고, 6월 말부터 8월 초까지 군산연안의 천해역에 많은 유어가 몰려와 성장하였다. 감성돔의 유어가 주로 서식하는 곳은 천해역의 사니질에 솟아있는 바위 및 암벽 주변이었으며, 이곳의 수온은 $23.7^{\circ}C{\sim}28.2^{\circ}C$, 염분은 30.1~32.2%의 범위를 나타냈다. 유어가 천해역에 처음 출현하는 6월 하순에 전장은 12.7~26.7mm(평균 16.5mm)였으며, 7월 중순에는 19.9~5 1. 4mm(33.4), 7월 말에 24.8~75.0mm(45.4), 8월 중순에 63.3~79.6mm(65.9mm)였다.8월 중순 이후에 천해역에서 감성돔의 유어는 채집되지 않았고, 8월 말부터는 고군산열도 부근에서 낭장망에 의해 전장 100mm 이상의 개체들이 채집되었다. 따라서 감성돔은 전장 70mm를 넘게 되는 8월 이후에는 천해역에서 연안의 외해쪽으로 이동하여 성장하는 것으로 사료된다.

• 한국응용곤충학회지
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• 제55권1호
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• pp.67-71
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• 2016

자나방과(Geometridae), 가지나방아과(Ennominae)에 속하는 줄마디가지나방(Chiasmia cinerearia)은 중부지방의 회화나무 가로수 및 조경수를 가해하는 대표적인 수목 해충이다. 본 연구의 결과 줄마디가지나방은 주로 잎 위에 250~300개의 알을 무더기로 산란하였으며, 유충은 총 5령기를 거쳐 용화되었다. 성충은 5월 초순과 7월 말경에 2회 우화하였으며, 1세대 경과일수는 약 48일로 조사되었다. 유충의 몸길이는 25~30 mm 정도이며 연녹색을 띠고 복부의 옆선은 황색이었다. 성충의 날개편 길이는 30~35 mm 정도이며 회갈색을 띠며 앞날개 외횡선을 중심으로 4각형의 무늬가 무리지어 있는 것이 특징이다.

• 한국수산과학회지
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• 제22권5호
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• pp.332-341
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• 1989

경상남도 일광 연안으로 흘러내리는 하천의 하구 기수역에서 미끈날망둑온 채집하여 이석과 위내용물을 분석하여, 자치어기의 나이, 성장율 및 식성을 추정하였다. 이석 미세성장선의 하루주기성은 약 15일 간격으로 연속하여 채집된 어류 이석을 각 채집일을 감안하여 성장선 배열의 공통성을 비교 분석하여 증명하였다. 체장의 성장은 Gompertz의 식으로 유의하게 표시되었다. $L=5.73{\cdot}\;e^{15.06}(1-e^{-0.0015t})$관찰 범위에서 체장의 일일성장율은 부화 후 $50\~60$일 사이에는 0.40 mm/day로 작았으나 자라면서 빨라져 $90\~100$일 사이에는 0.85 mm/day로 증가하였다. 위내용물 분석 결과, 미끈날망둑은 전형적인 육식성 어종으로 밝혀졌는데, 주된 먹이생물은 polychaetes, amphipods, copepods 였으며 이들은 위내용물의 대부분을 차지하였다. 미끈날망둑은 성장에 따라 다소 먹이 선택에 있어 변화를 보이는데, $15\~30$mm 크기군에서는 copepods가 비교적 많이 섭이 되었지만, 성장함에 따라 amphipods의 섭이가 증가되었다. 한편 polychaetes의 선호도는 모든 크기군에 걸쳐 가장 높았다.

• Journal of Microbiology and Biotechnology
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• 제17권4호
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• pp.547-559
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• 2007

Bacillus thuringiensis (Bt) was first described by Berliner [10] when he isolated a Bacillus species from the Mediterranean flour moth, Anagasta kuehniella, and named it after the province Thuringia in Germany where the infected moth was found. Although this was the first description under the name B. thuringiensis, it was not the first isolation. In 1901, a Japanese biologist, Ishiwata Shigetane, discovered a previously undescribed bacterium as the causative agent of a disease afflicting silkworms. Bt was originally considered a risk for silkworm rearing but it has become the heart of microbial insect control. The earliest commercial production began in France in 1938, under the name Sporeine [72]. A resurgence of interest in Bt has been attributed to Edward Steinhaus [105], who obtained a culture in 1942 and attracted attention to the potential of Bt through his subsequent studies. In 1956, T. Angus [3] demonstrated that the crystalline protein inclusions formed in the course of sporulation were responsible for the insecticidal action of Bt. By the early 1980's, Gonzalez et al. [48] revealed that the genes coding for crystal proteins were localized on transmissible plasmids, using a plasmid curing technique, and Schnepf and Whiteley [103] first cloned and characterized the genes coding for crystal proteins that had toxicity to larvae of the tobacco hornworm, from plasmid DNA of Bt subsp. kurstaki HD-1. This first cloning was followed quickly by the cloning of many other cry genes and eventually led to the development of Bt transgenic plants. In the 1980s, several scientists successively demonstrated that plants can be genetically engineered, and finally, Bt cotton reached the market in 1996 [104].