• Journal of fish pathology
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• v.31 no.2
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• pp.81-85
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• 2018

Fetal bovine serum (FBS) is an essential element of cell growth and can also affect the viral replication. In this study, we tried to find out whether FBS concentration affects the viral infectivity titer of IHNV and IPNV. EPC cells were suspended with MEM supplemented with various concentrations of FBS (MEM0, MEM2, MEM5 and MEM10) and cultured in 96-well plate. Each virus was 10-fold diluted virus and inoculated in 96-well plate. The highest infectivity titer of IHNV was $10^{7.88}\;TCID_{50}/mL$ in 96-well plate using MEM5 and the lowest one was $10^{7.30}\;TCID_{50}/mL$ in 96-well plate using MEM10. The highest infectivity titer of IPNV was $10^{7.47}\;TCID_{50}/mL$ in 96-well plate using MEM5 and the lowest one was $10^{6.97}\;TCID_{50}/mL$ in 96-well plate using MEM10. This study showed that not only 0% FBS but 10% FBS leads low infectivity titer of IHNV and IPNV. Therefore, it is considered that the desirable concentration of FBS is 2% or 5% for measurement of infectivity titer of IHNV and IPNV.

• Journal of Plant Biotechnology
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• v.46 no.2
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• pp.97-105
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• 2019

Dehydration Responsive Element Binding (DREB) gene is one of the essential transcription factors plants use for responding to stress conditions including salinity, drought, and cold stress. The purpose of this study was to isolate the full length and characterize the DREB gene from three different genotypes of sugarcane, wild, commercial cultivar, and interspecific hybrid sugarcane. The length of the gene, designated ScDREB was 789 bp, and coding for a putative polypeptide of 262 amino acid residues. Sequences of the gene were submitted to the GenBank database with accession numbers of KX280722.1, KX280721.1, and KX280719.1 for wild sugarcane, commercial cultivar (KPS94-13), and interspecific hybrid (Biotec2), respectively. In silico characterization indicated that the deduced polypeptide contains a putative nuclear localization signal (NLS) sequence, and a conserved AP2/ERF domain of the DREB family, at 82-140 amino residues. Based on multiple sequence alignment, sequences of the gene from the three sugarcane genotypes were classified in the DREB2 group. Gene expression analysis indicated, that ScDREB2 expression pattern in tested sugarcane was up-regulated by salt stress. When the plants were under 100 mM NaCl stress, relative expressions of the gene in leaves was higher than those in roots. In contrast, under 200 mM NaCl stress, relative expressions of the gene in roots was higher than those in leaves. This is the first report on cloning the full length and characterization, of ScDREB2 gene of sugarcane. Results indicate that ScDREB2 is highly responsive to salt stress.

• Journal of the Korea Convergence Society
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• v.10 no.7
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• pp.281-287
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• 2019

Application of heat recovery system applying air supply and cexhaust ventilation device essential in energy management system for the optimum ventilation system utilization and energy saving. This is a key element of infrastructure technology for high-efficiency energy buildings, because it can save heating and cooling energy in winter and summer. In this paper, heat transfer efficiency was simulated using paper, plastic, and aluminum materials that was examined to compare heat exchanger performance under uniform flow conditions. We tested heat transfer efficiengy according to the shape of two of that, one is orthogonal and the other is refraction shape. Based on the simulation results, it is expected to contribute to the production of high performance heat exchanger with heat transfer performance and pressure loss.

• Advances in materials Research
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• v.8 no.1
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• pp.47-73
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• 2019

Joining of Mg/Ti hybrid structures by welding for automotive and aerospace applications has attracted great attention in recent years due mainly to its potential benefit of energy saving and emission reduction. However, joining them has been hampered with many difficulties due to their physical and metallurgical incompatibilities. Different joining processes have been employed to join Mg/Ti, and in most cases in order to get a metallurgical bonding between them was the use of an intermediate element at the interface or mutual diffusion of alloying elements from the base materials. The formation of a reaction product (in the form of solid solution or intermetallic compound) along the interface between the Mg and Ti is responsible for formation of a metallurgical bond. However, the interfacial bonding achieved and the joints performance depend significantly on the newly formed reaction product(s). Thus, a thorough understanding of the interaction between the selected intermediate elements with the base metals along with the influence of the associated welding parameters are essential. This review is timely as it presents on the current paradigm and progress in welding and joining of Mg/Ti alloys. The factors governing the welding of several important techniques are deliberated along with their joining mechanisms. Some opportunities to improve the welding of Mg/Ti for different welding techniques are also identified.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.12 no.4
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• pp.387-394
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• 2019

The purpose of this study is to analyze and identify the strengths of companies selected in each field based on data released by the IOTONE to see if the most influential companies in the industrial Internet of Things are gaining high competitiveness by exercising their capabilities in each field. The industrial Internet of Things is emerging as an essential element in the overall realm of business activity, as well as to make manufacturing factories competitive smart factories. Appropriate platforms should be applied to apply the industrial Internet of Things throughout the enterprise. Globally, many companies are proposing a platform for the industrial Internet of Things. We analyzed the strengths of the most influential companies in each field of connected machines, cybersecurity, analysis platforms, embedded computing, platform connectivity and hardware connectivity in the industrial Internet of Things, and looked at trends such as the current state of operations and the characteristics of the platform.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.32 no.4
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• pp.233-240
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• 2019

Open-hole tensile tests are usually performed to measure the tensile strengths of composites as they are an essential parameter for designing composite structures. However, correctly modeling the tensile test is extremely challenging as it involves various damages such as fiber and matrix damage, delamination, and debonding damage between the fiber and matrix. Therefore, a progressive damage model was developed in this study to estimate the in-plane failure and delamination between the fiber and matrix. The Hashin damage model and cohesive zone approach were used to model ply and delamination failures. The results of the present model were compared with previously published experimental and numerical findings. It was observed that neglecting delamination during finite element analysis led to overestimation of tensile strength.

• Journal of IKEEE
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• v.23 no.1
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• pp.143-150
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• 2019

The back-to-back HVDC asynchronous grid interconnect technology has problems of high harmonic generation, high cost, and low scalability. To solve this problem, research on asynchronous grid interconnect technology using VFT is actively being conducted. However, the reactive power due to the inductance component of the VFT is generated, and the problem of additional installation of the reactive power compensating facility is inevitably generated. Therefore, in this paper, we aim to solve the reactive power compensation problem of existing VFT by designing rectifier AC-DC converter, which is an essential element of the asynchronous grid connection system using VFT, to compensate reactive power as well as active power supply. The performance was verified through simulation.

• Journal of Applied Biological Chemistry
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• v.62 no.1
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• pp.93-100
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• 2019

The root of Panax ginseng is used in ethnomedicine throughout eastern Asia and various recent studies have proved that Panax ginseng has inhibitory effects on cardiovascular disease. Each factor causing cardiovascular disease is known to have a very complex process which is achieved by a diverse number of mechanisms. Among these factors, platelets are the most important because they directly participate in thrombogenesis. Therefore, inhibiting the activity of platelets is an essential element for prevention of cardiovascular diseases. Our previous study showed the antiplatelet effects of Korean red ginseng extract and two of its components, ginsenoside Rg3 and ginsenoside Ro. However, the inhibitory mechanism of other ginsenosides remains unclear. Therefore, we investigated the inhibitory mechanism of ginsenoside F4 (G-F4) from Korean red ginseng on the regulation of signaling molecules involved in human platelet aggregation. With the use of G-F4, collagen-induced human platelet aggregation was inhibited in a dose-dependent manner, and it suppressed collagen-induced elevation of $[Ca^{2+}]_i$ mobilization through elevated phosphorylation of inositol 1, 4, 5-triphosphate receptor I ($Ser^{1756}$). In addition, G-F4 inhibited fibrinogen binding to ${\alpha}IIb/{\beta}_3$ during collagen-induced human platelet aggregation. Thus, in the present study, G-F4 showed an inhibitory effect on human platelet activation, suggesting its potential use as a new natural medicine for preventing platelet-mediated cardiovascular diseases.

• Geophysics and Geophysical Exploration
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• v.22 no.1
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• pp.12-20
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• 2019

The first-arrival traveltime calculation method based on the damped wave equation overcomes the shortcomings of ray-tracing methods. Since this algorithm needs to solve the damped wave equation, numerical modeling is essential. However, it is not desirable to use the finite-difference method (FDM), which has good computational efficiency, for simulating the land seismic data because of irregular topography. Thus, the finite-element method (FEM) which requires higher computational cost than FDM has been used to correctly describe the irregular topography. In this study, we computed first-arrival traveltimes in an irregular topographic model using FDM incorporating embedded boundary method (EBM) to overcome this problem. To verify the accuracy and efficiency of the proposed algorithm, we compared our results with those of FEM. As a result, the proposed method using EBM not only provided the same accuracy as the FEM but also showed the improved computational efficiency.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.46 no.11
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• pp.952-960
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• 2018

Recently, lots of remote sensing satellite require agility to collect more images within the limited time frame. To satisfy this kind of mission requirement, high torque actuator such as CMG is an essential element. In this study, 5Nm class small CMG developed by KARI is introduced to implement for an agile small satellite design. One of the singularity escape CMG steering law, Designated Direction Escape (DDE) method, which is a sort of modified version of Singular Direction Avoidance (SDA) method is summarized for its application on the numerical simulation of agile attitude control system design result. The performance of DDE method is demonstrated properly by escaping well known elliptic internal singularity successfully. 5Nm class small CMG cluster in a pyramid type as well as a roof type configuration is utilized to perform the numerical simulation and to demonstrate its agility design result for a small satellite. Simulation result shows the properness of 5Nm small CMG to a small agile satellite system. Also, the simulation result provides some valuable information that is important to CMG hardware design and manufacturing.