Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Effects of fetal bovine serum concentrations on viral infectivity titers of infectious hematopoietic necrosis virus and infectious pancreatic necrosis virus

Fetal bovine serum의 농도에 따른 infectious hematopoietic necrosis virus와 infectious pancreatic necrosis virus의 감염가 변화

  • Kim, Hyoung Jun (OIE reference laboratory for VHS, National Fishery Products Quality Management Services) ;
  • Park, Jeong Su (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Kwon, Se Ryun (Department of Aquatic Life Medical Sciences, Sunmoon University)
  • 김형준 (VHS OIE 표준실험실, 국립수산물품질관리원) ;
  • 박정수 (선문대학교 수산생명의학과) ;
  • 권세련 (선문대학교 수산생명의학과)
  • Received : 2018.11.25
  • Accepted : 2018.12.05
  • Published : 2018.12.31
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Abstract

Fetal bovine serum (FBS) is an essential element of cell growth and can also affect the viral replication. In this study, we tried to find out whether FBS concentration affects the viral infectivity titer of IHNV and IPNV. EPC cells were suspended with MEM supplemented with various concentrations of FBS (MEM0, MEM2, MEM5 and MEM10) and cultured in 96-well plate. Each virus was 10-fold diluted virus and inoculated in 96-well plate. The highest infectivity titer of IHNV was $10^{7.88}\;TCID_{50}/mL$ in 96-well plate using MEM5 and the lowest one was $10^{7.30}\;TCID_{50}/mL$ in 96-well plate using MEM10. The highest infectivity titer of IPNV was $10^{7.47}\;TCID_{50}/mL$ in 96-well plate using MEM5 and the lowest one was $10^{6.97}\;TCID_{50}/mL$ in 96-well plate using MEM10. This study showed that not only 0% FBS but 10% FBS leads low infectivity titer of IHNV and IPNV. Therefore, it is considered that the desirable concentration of FBS is 2% or 5% for measurement of infectivity titer of IHNV and IPNV.

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References

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