• Plant Breeding and Biotechnology
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• v.6 no.4
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• pp.404-412
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• 2018

The influenza neuraminidase (NA, E.C. 3.2.1.18), an antiviral, has been the target of high pharmaceutical companies due to its essential role in viral replication cycle. Perilla frutescens (P. frutescens) is used in traditional Chinese medicine for various diseases, such as cold due to wind-cold, headache and cough. In this context, four major polyphenolic compounds including rosmarinic acid-3-O-glucoside (1), rosmarinic acid (2), luteolin (3), and apigenin (4) isolated from P. frutescens were evaluated for their inhibitory effect on recombinant virus H1N1 neuraminidase (rvH1N1 NA). Among the test compounds, rosmarinic acid and luteolin inhibited the rvH1N1 NA with an $IC_{50}$ of 46.7 and $8.4{\mu}M$, respectively. The inhibition kinetics analyzed by the Dixon plots indicated that rosmarinic acid and luteolin were noncompetitive inhibitors and that the inhibition constant, $K_I$, was established as 43.9 and $14.3{\mu}M$, respectively. In addition, 578 genetically diverse accessions and 39 cultivars of P. frutescens were analyzed using HPLC to characterize the diversity of polyphenolic composition and concentration. The individual and total compositions exhibited significant difference (P < 0.05), especially rosmarinic acid which was detected as the predominant metabolite in all accessions (58.8%) and cultivars (62.8%). Yeupsil and Sangback cultivars exhibited the highest rosmarinic acid ($3,393.5{\mu}g/g$) and luteolin ($383.3{\mu}g/g$) content respectively. YCPL177-2 with the high concentration ($889.8{\mu}g/g$) of luteolin may be used as a genetic resource for breeding elite cultivars.

• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.137-142
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• 2019

Gene edited crops can be classified as SDN-1, SDN-2 and SDN-3 group depending on their mutation's range and the usage of donor DNA. The SDN-1 and SDN-2 crops, in particular, could be developed as 100% transgene-free, which do not contain any DNA fragment of the vector or guide RNA used for gene editing such as CRISPR Cas9 system. Therefore, there are no scientific methods available for the detection of these crops and differentiation with the one produced by conventional cross breeding techniques. Additionally, it would be impossible to properly implement the existing GMO regulation law, in particular, the national legislation for "GMO labelling". In this regard, Australia has announced that SDN-1 crops will not be subjected to the existing GMO regulation. Furthermore, Argentina and Brazil have established a new policy that GE crops with no transgene (100% transgene-free crops) should be exempted from the scope of the GMO. In addition, Japan has also announced that "an organism that has no remnants of inserted nucleic acid processed extracellularly is not subjected to the Cartagena Act". It means that SDN-2 crops can also be exempted from the scope of GMO. In this trend, in South Korea, I suggested that gene edited crops with no remnants of inserted foreign DNA fragments should be excluded from the existing GMO regulation. Thus, I expect that diverse elite crop lines should be developed by using advanced gene editing technologies

• Convergence Security Journal
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• v.17 no.5
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• pp.143-151
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• 2017

The purpose of this study is to provide the future development of the PRC Marine Corps by analyzing the changing functions via its historical development. The PRCMC is an elite regular military forces and was established by the Central Military Commission(China) in 1953 in order to project the national military power toward the enemy's territory by overcoming maritime obstacles such as seas and lakes. The PRCMC is relatively smaller personnel strength compared with the whole size of the PRC military forces. Thus its functions were limited in the areas of amphibious and land operations, island and land defense against Taiwan before the PRC pursues expanding policy toward outside. However, in the 2000s, China pursues its policy for obtaining absolute national interest so that its functions are rapidly enlarged into defense of the forward naval bases, and those for evolving its power toward outside according to not only the island territorial dispute with Japan, but also Xi Jinping's active expanding policy what we call 'the one belt and one road'. So its personnel strength is slowly increased. If the increasement of the PRC Navy and Marine Corps would develop into the level of which they can contain the status and influence of the US military power in Asia-Pacific area, it is possible that the security environment of the North-east Asia including the Korean peninsula will be fluctuated. Consequently, the ROK also needs to reevaluate the functions and the size of the joint strength of the ROK Navy and Marine Corps considering the transition of the changing security environment.

• Journal of Plant Biotechnology
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• v.46 no.1
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• pp.32-36
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• 2019

Calla lilies (Zantedeschia spp.) are monocotyledonous ornamental plants which belongs to the Araceae family. After the release of elite calla cultivar, an efficient propagation system is needed for commercial use. Despite the use of conventional propagation methods such as splitting of tubers and rhizomes of calla, rapid and efficient propagation system should be developed. In order to achieve this goal, stem segments contained apical meristems derived from calla lily cultivar (cv. Gag-si) were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of cytokinin and auxin. This was aimed at inducing embryogenic calluses, shoots and multiple shoots. As a result, about 25% of induction rates of yellow embryogenic calluses were observed with MS medium containing both $0.5mg{\cdot}L^{-1}\;NAA$ and $1.5mg{\cdot}L^{-1}\;BA$ as growth regulators. In the experiments involving the regeneration from embryogenic calluses through shoot formation, MS medium supplemented with $0.5mg{\cdot}L^{-1}\;IAA$ and $2.0mg{\cdot}L^{-1}\;BA$ showed the highest rates at approximately 85 ~ 90% with regard to the formation of shoots in calla. Moreover, multiple shoots needed for rapid propagation were generated when explants were cultured on MS medium supplemented with $0.5mg{\cdot}L^{-1}\;IAA$ and $2.0mg{\cdot}L^{-1}\;BA$ with 40% of formation rate. In this study, the combination of auxin and cytokinin showed positive effects on both the induction of embryogenic calluses, the formation of shoots as well as multiple shoots in calla. The regeneration system described here can contribute to the development of breeding programs of calla in the future.

• Journal of The Korean Association For Science Education
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• v.39 no.2
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• pp.233-248
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• 2019

This study shows that the Argovian Cantonal School in Aarau, Switzerland, which Albert Einstein attended from 1895 to 1896, had been closely related to the ideological education controversy in German Gymnasium throughout the 19th century. Due to this controversy, Einstein hardly received a formal science education in Bavaria. Despite the lack of formal education in Germany, he had a habit of self-studying from an early age and continued with this practice all through his life. He had a hard time at the authoritarian school in Munich, but at the democratic school in Aarau, where freedom and autonomy were secured, he was able to achieve emotional stability. For a long time, the city Aarau prevailed as a location of tolerance and multi-culturalism, without religious, regional, and national discrimination. This was possible due to the influence of external and unrestricted social mobility, as well as the Enlightenment from France. As a result, this small public school was able to acquire a mass of qualified human resources from outside of Switzerland. As a consequence of the controversy regarding the educational ideology, the Cantonal School adopted practical thoughts and the Enlightenment that fit the spirit of the times. The school consisted of two independent educational organizations: the Gymnasium, where the 'neuhumanistsch' education for the elite training was conducted, and the 'Gewerbeschule', where a more realistic education system was set up to suit the citizen life. In particular, after 1835, the Gymnasium changed gradually from the pure humanistic education to the 'utraquistisch' ways by introducing practical subjects such as natural history. Thereafter, the Cantonal School became an institution that was able to achieve a genuine humanity, academic, and civic life education. Einstein, who attended the 'technische Abteilung' of the 'Gewerbeschule,' considered this school as a role model of an institution that realized true democracy, and that left an unforgettable impression on him.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.364-377
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• 2018

Hexaploid wheat (common wheat/bread wheat) is one of the most important cereal crops in the world and a model for research of an allopolyploid plant with a large, highly repetitive genome. In the heritability of agronomic traits, variation in gene presence/absence plays an important role. However, there have been relatively few studies on the variation in gene presence/absence in crop species, including common wheat. Recently, a reference genome sequence of common wheat has been fully annotated and published. In addition, advanced next-generation sequencing (NGS) technology provides high quality genome sequences with continually decreasing NGS prices, thereby dawning full-scale wheat functional genomic studies in other crops as well as common wheat, in spite of their large and complex genomes. In this review, we provide information about the available tools and methodologies for wheat functional genomics research supported by NGS technology. The use of the NGS and functional genomics technology is expected to be a powerful strategy to select elite lines for a number of germplasms.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.516-521
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• 2018

"Nulichal" was developed by the National Institute of Crop Science, RDA, in 2010. The initial cross was conducted in 2000, and an elite line (HB15305-B-B-31-2) was selected in 2005. Subsequently, preliminary yield and advanced yield trials have designated it as "Iksan 95." It showed good agronomic performance in regional yield trials from 2008 to 2010 and was released as "Nulichal," with resistance against the barley yellow mosaic virus (BaYMV) and high yields with a waxy endosperm. The average heading and maturation dates of "Nulichal" were April 24 and May 30, respectively in paddy fields, which were 1 day later than those of the check cultivar "Saechalssalbori." It had culm and spike lengths of 82 and 4.0 cm, respectively. It showed $774spikes/m^2$, 63 grains/spike, 25.0 g of 1,000-grain weight, and 793 g of test weight. "Nulichal" showed higher levels of resistance to BaYMV than the naturally occurring Iksan (type III strain), Naju (type I), and Jinju (type II). The expansion rate was higher than that of "Saechalssalbori" and its hardness, gumminess, and chewiness were lower than those of "Saechalssalbori." Its average pearled grain yield in the regional yield trial was 4.00 MT/ha in paddy fields, which was 10% higher than that of the check cultivar. The combined availability of "Nulichal" was improved by its increased culm length compared with that of "Hinchalssal."

• Cross-Cultural Studies
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• v.49
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• pp.201-228
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• 2017

In Korean society, universities represent institutions of higher education. Industrialization and economic development led to a small number of elite groups at the helm of these institutions. However, our society has encouraged a lifelong system of learning, and apparently, the present university system does not represent an ideal scenario. The Korean government recognized the need for appropriate changes. The events associated with the implementation of related policies occurred at the Ewha Womans University (Seoul) last year. This article is based on the notion of lifelong education to further our understanding of the role of university in the absence of a consensus among university and college members regarding lifelong learning in our society. As an alternative, we looked at the case of France, which is adopting a lifelong education system and implementing related policies ahead of us. Despite regulatory challenges and adaptation of the role of public education in lifelong learning, France has laid a solid foundation. In our case, we are recognizing the need to prepare for lifelong learning. In particular, it is necessary to increase public awareness of education by clearly recognizing our national responsibility and increasing the financial support to universities, accordingly. Above all, the role and attitude of universities must change, along with the perception of its members.

• Journal of Korea Entertainment Industry Association
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• v.15 no.5
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• pp.85-98
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• 2021

The present thesis is a study about a character actor's characterization from the concept of 'star persona'. Conducted in 2020, this is a follow-up study about a personality actor's characterization. Lee Sung-min was selected due to his wide acting spectrum among domestic actors and his brilliant expression of differing characters as a character actor. The study discusses characterization methods for character actors on the basis of his role of high-ranking North Korean bureaucrat 'Ri Myung-un' in the film 'The Spy Gone North'. Stanislavski claimed that character building or characterization was the pinnacle of the acting art. However, such an expression of personality requires hard work and devotion from an actor. The actor Lee Sung-min practiced the North Korean dialect in order to act the role, and added the way the elite speak in North Korea to this dialect. He achieves a style of acting that raises tensions within the drama by spitting out lines in one breath and by making the audience fixate their gaze within character solo shots for which the character's emotions and lines serve as the core, with the purpose of maximizing dramatic tension and situational reality. Continuity and angle also serve extremely important roles in terms of expressing a character's personality development, affect, and emotions. The present thesis discussed the character development methods for a character actor's role of 'Ri Myung-un' in the film 'The Spy Gone North'.

• Knowledge Management Research
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• v.23 no.3
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• pp.111-128
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• 2022

The purpose of this study is to identify the difference in happiness of workers depending on the perceived leadership types in a big company in Korea. The research objectives were 1) to identify the managers' leadership types which their workers perceive in a big corporate organization, and 2) to analyze the difference in a degree of workers' happiness according to the types of manager's leadership. To this end, a latent profile analysis was conducted. The research results are as follows. There were four types of leadership: 1) the Elite type which shows perfect ability in each area of self-management, task management, people management, and organization management, 2) the Competent type which has excellent self-management skills, good levels of task management and organizational management skills, but relatively poor people management skills, 3) the Self-centered type with the average levels of self-management, task management and organization management but with the poor level of people management skills, and lastly 4) the Passive type with the average level of self-management, somewhat low levels of task management and organization management skills and the poor level of people management skills. In addition, there was a statistically significant difference in the degree of happiness of workers according to the manager's leadership type. This study is meaningful in that it presented the leadership types of managers in a big company through the latent profile analysis and provided its implications on the workers' happiness.