• Journal of Mushroom
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• v.18 no.3
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• pp.268-273
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• 2020

This study aimed to assess the feasibility of composting spent mushroom substrate (SMS) materials of Lentinula edodes (Le), Hericium erinaceus (He), and Pleurotus ostreatus (Po). The different SMSs were composted for 7 to 10 days at high temperatures over 50℃; the composting procedure was completed in 30 days. A maturity test was conducted using the radish seed germination index and CoMMe-100. The composted SMS (CSMS) from Le and He showed gemination indices of 130% and 81%, respectively, that satisfied the criteria of maturity standard (gemination index over 70%) and the CoMMe-100 analysis. The physicochemical changes of CSMSs included an increase in the pH range from 4-5 to 6-7, slight reduction in the EC to 1-1.4 dS/m, and an organic content of 36.9% in LeCSMS. In LeCSMS, the contents of N, P, and K were 1.2%, 2.3%, and 0.77%, respectively, and heavy metals were detected below the standard value in all CSMSs; the Ca and Mg contents in the CSMSs were increased from 30% to 60% when compared to those in the SMSs. The C/N ratio (from 26-33) in LeSMS and HeSMS decreased to 15.3-15.9 in CSMSs. The growth effect of LeCSMS treatment on pepper seedlings was 60% higher than that in the control groups, one of which was treated with commercial organic compost; the former showed a superior growth effect on the leaf width, leaf length, and leaf number compared to other control groups. In conclusion, LeCSMS and HeCSMS could be utilized as compost resources capable of efficient soil amendment and plant growth promotion.

• Journal of Bio-Environment Control
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• v.21 no.4
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• pp.348-353
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• 2012

This study was conducted to figure out the possibility of non-drainage in muskmelon (Cucumis melo L.) hydroponics culture. Plants were grown under 3 different levels of drainage, standard (20~40%, SD), minimum (5~10%, MD), and non-drainage (ND). Throughout cultivation periods, constant water content and electrolyte conductivity changes in root zone were observed in SD in the range of 60~70% and $1.5{\sim}2.5dS{\cdot}m^{-1}$, respectively. ND treatment caused the fluctuation in water content and electrolyte conductivity of root zone and its change ranges were 30~50% in water content and $2{\sim}6dS{\cdot}m^{-1}$ in electrolyte conductivity, but ND treatment did not decrease fruit quality. Even if fruit fresh weight was slightly lower in ND with 1,863 g, than in SD with 1,990 g, the fruit weight in ND meets standard market size, 1,800~2,000 g. Higher soluble solids content was observed in fruit in ND than in SD and MD. Total amount of drainage per plant was 27,718, 15,769 and 2,346 mL in SD, MD and ND, respectively. SD showed $83.2m^3$ drainage, 34.5% drainage of irrigation amount whereas required total irrigation amount in ND was very low with $7m^3$.

• Journal of Marine Life Science
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• v.9 no.1
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• pp.9-21
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• 2024

Paralytic shellfish poisoning (PSP) including Saxitoxin (STX) is caused by harmful algae, and poisoning occurs when the contaminated seafood is consumed. The mouse bioassay (MBA), a standard test method for detecting PSP, is being sanctioned in many countries due to its low detection limit and the animal concerns. An alternative to the MBA is the Neuro-2a cell-based assay. This study aimed to establish various test conditions for Neuro-2a assay, including cell density, culture conditions, and STX treatment conditions, to suit the domestic laboratory environment. As a result, the initial cell density was set to 40,000 cells/well and the incubation time to 24 hours. Additionally, the concentration of Ouabain and Veratridine (O/V) was set to 500/50 μM, at which most cells died. In this study, we identified eight concentrations of STX, ranging from 368 to 47,056 fg/μl, which produced an S-shaped dose-response curve when treated with O/V. Through inter-laboratory variability comparison of the Neuro-2a assay, we established five Quality Control Criteria to verify the appropriateness of the experiments and six Data Criteria (Top and Bottom OD, EC₅₀, EC₂₀, Hill slop, and R² of graph) to determine the reliability of the experimental data. The Neuro-2a assay conducted under the established conditions showed an EC₅₀ value of approximately 1,800~3,500 fg/μl. The intra- & inter-lab variability comparison results showed that the coefficients of variation (CVs) for the Quality Control and Data values ranged from 1.98% to 29.15%, confirming the reproducibility of the experiments. This study presented Quality Control Criteria and Data Criteria to assess the appropriateness of the experiments and confirmed the excellent repeatability and reproducibility of the Neuro-2a assay. To apply the Neuro-2a assay as an alternative method for detecting PSP in domestic seafood, it is essential to establish a toxin extraction method from seafood and toxin quantification methods, and perform correlation analysis with MBA and instrumental analysis methods.

• Journal of Korean Society of Steel Construction
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• v.20 no.3
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• pp.365-375
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• 2008

The main objective of this paper is to study the behaviour of yLRC composite columns at elevated temperatures by experimental test. The effects of load ratios, cross-section size and fire protection for the yLRC columns were investigate d by the test and compared using the heat transfer analysis perfo rmed based on the finite element program ANSYS 10.0 using the ISO834 standard fire curve, following the main guidelines proposed by the EC4 Part 1.2. As heat transfer is the movement of heat by conduction, convection, and radiation, and as temperature inside an object varies by position and time, time. As the steel's thermal conductivity is higher than that of concrete, steel loses its strength rapidly in a high-temperature situation such as a fire. Fire resistance performance of the yLRC composite column under fire conditions was evaluated througheat transfer analysis for parametric study.

• Journal of Korean Society on Water Environment
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• v.20 no.1
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• pp.48-54
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• 2004

Two methods, a Ceriodaphnia algal uptake suppression test (CAUST) and a new toxicity test based on temperature control (TTBTC) which are based on feeding behaviour and temperature control, respectively, were developed and compared for the adoption as the better methodology for short-term toxicity screening. As previously published by Lee et aI., (1997), the CAUST method is based on the feeding behaviour of C. dubia and requires as little as 1 hour of contact time between C. dubia neonates and toxicant. However, even though CAUST requires only 1 hour of contact time, this method still take many hours for the preparation and measurement. Before the test starts, neonate digestive tracts were cleared by feeding yeast to the daphnids, Neonates were then exposed to toxicant, followed by addition of Scenedesmus subspiatus into the bioassay vessels. Daphnids were examined under the bright-field microscope with the presence of algae (indicated by a green colored digestive tract) or the absence of algae. Uptake indicated no toxic effect, whereas, absence of uptake indicated toxic inhibition. Unlike CAUST, the newly developed method (TTBTC) is based on just temperature control for the toxicity test of C. dubia. Initially, neonates are exposed to toxicants while the temperature of water bath containing media increased to $35.5^{\circ}C$. After 1.25 hour of contact time, the number of the daphnids, either live (no toxic effect) or dead (toxic effect), is counted without the aid of any instrument. In both methods, median effective concentrations ($EC_{50}$ values) were computed based on the results over a range of dosed toxicant concentrations. It showed that TTBTC was as sensitive as the standard 48-hour acute bioassay and CAUST. TTBTC and CAUST were much more sensitive than the I-hour I.Q. test and 30-minute Microtox. This study indicates that TTBTC is an easier and more rapid toxicity test than the standard 48-hour acute bioassay and even CAUST.

• Journal of Korean Society on Water Environment
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• v.33 no.2
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• pp.181-186
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• 2017

This paper aimed to evaluate the results of alternative acute toxicity test on 83 wastewater samples. Each sample was tested by traditional method (using laboratory cultured D. magna as a test organism) and alternative method (using Toxikit which can be purchased from a specialized company). The relationship between Lab. culture toxicity and Toxkits toxicity indicated good relation ($r^2=0.84$, p<0.01, n = 83). Number of samples which showed a small difference of lower than 0.5 between two test methods were 52 and they account for 63 percent of collected samples. In addition, these 52 samples had a lower average toxicity of TU 0.5 (Lab. culture method) and TU 0.45 (Toxkits method). Whereas samples which indicated big difference of test results between two methods, had a tendency to show higher toxicity. From these results, alternative toxicity test method could be applied to the official test method, if samples would have a lower toxicity less than TU 2. Also, Toxikit standard toxicant test results indicated $EC_{50}$ values between 0.93 and 1.68 mg/L and these results were considered as valid for quality control standard.

• Journal of Environmental Analysis, Health and Toxicology
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• v.21 no.4
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• pp.209-219
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• 2018

In the areas of RoHS, WEEE, ELV and REACH, reinforcement of environmental regulations against harmful substances is a global trend not only in EC but also in all over the world. In the fields of Korea's major export products such as material parts, electrical and electronic products and automobile parts, we are responding to these regulations consistently. To develop reference material for analyzing lead and cadmium in paints, the candidate materials were produced through the screening process which separated shapes and sizes. To secure the traceability of the candidate materials produced, the characteristics and uncertainties are estimated by ICP-AES analysis using the primary reference material. The short-term and long-term stabilities also are evaluated in parallel. In order to calculate the final certification value of the candidate material, the verification were carried out by the performance evaluation through the comparison among the KOLAS (Korea Laboratory Accreditation Scheme) laboratories, and the CRM was produced in accordance with ISO Guide 35. The certified values and uncertainties of Pb and Cd of the final paint standard, determined according to the joint analysis among laboratories, are Pb [($191.4{\pm}3.1$) mg/kg, ($944.1{\pm}5.6$) mg/kg] and Cd [($45.0{\pm}2.6$) mg/kg, ($225.5{\pm}3.5$) mg/kg]. These standard materials were developed to enhance the reliability of measurement analysis, including the validity and traceability of measurement results. Also it is expected that the CRM will be used as QCM (quality control material) for the product design and the process monitoring, so that regulation and management of hazardous heavy metals can be systematically implemented.

• Clean Technology
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• v.15 no.1
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• pp.23-30
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• 2009

European directive DIRECTIVE 2002/96/EC requires the minimum recycling & recovery rate on the waste electrical and electronic equipments (WEEE). But, they do not have guidelines on the calculation methods for recycling and recovery rate. A standard method to calculate recyclability and recoverability rate of products in the designing stage is necessary for the manufacturers so that they can reflect the calculated result to the improvement of product design. In this work, we investigated the existing calculation methods for the recycling and recovery rates of WEEE and the recyclability and recoverability rates of electrical and electronic equipments (EEE). A method for the calculation of recyclability and recoverability rates for the EEE products in the development stage was developed. The newly-developed calculation method was applied to some EEE products and the calculated results were evaluated.

• Journal of Bio-Environment Control
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• v.7 no.2
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• pp.123-129
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• 1998

This experiment was conducted to composition of nutrient solution for tore of endive hydroponics, and was compared the nutrient solution developed in the Seoul City University(SCUE) with the conventional solution of Proefstation voor Tuinbouw ender Glas. Proper compositions of nutrient solution for endive hydroponics were NO$_3$-N 15.0, NH$_4$-N 1.0, PO$_4$-P 3.0. K 10.0, Ca 5.0 and Mg 3.5 me.$\ell$$^{-1}$ , respectively SCUE and PTG standard nutrient solution showed slightly better yields among nutrient solutions. The SCUE nutrient solution and the PTG nutrient solution had no difference on growth and yield of endive Plants.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• autumn
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• pp.573-576
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• 2003

In these days. a lot of paper are made electronic document using the computer through the spread of computer and internet technology growing up. which is produced in construction project such as form document, report, account, specification and so on. Developed electronic document is distributed energetically through internet. Because existing electronic document is composed of various format, it is difficult that document data is shared and exchanged among the project participating groups in internet. So, it is required that construction-related technical document is made electronic document by standard information system and how to use technical document. Therefore, tins study presents the procedure and method of developing electronic document on technical document using XML technology.