Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
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Evaluation of Alternative Acute Toxicity Test Method with Daphnia magna

Toxkits에 의한 물벼룩 급성독성시험방법의 제도적용 적합성 평가

  • Kim, Jongmin (National Institute of Environmental Research Water Environmental Engineering Research Division) ;
  • Shin, Kisik (National Institute of Environmental Research Water Environmental Engineering Research Division) ;
  • Lee, Jungseo (National Institute of Environmental Research Water Environmental Engineering Research Division) ;
  • Lee, Taekjune (National Institute of Environmental Research Water Environmental Engineering Research Division)
  • 김종민 (국립환경과학원 물환경공학연구과) ;
  • 신기식 (국립환경과학원 물환경공학연구과) ;
  • 이정서 (국립환경과학원 물환경공학연구과) ;
  • 이택준 (국립환경과학원 물환경공학연구과)
  • Received : 2016.10.20
  • Accepted : 2017.03.20
  • Published : 2017.03.30
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Abstract

This paper aimed to evaluate the results of alternative acute toxicity test on 83 wastewater samples. Each sample was tested by traditional method (using laboratory cultured D. magna as a test organism) and alternative method (using Toxikit which can be purchased from a specialized company). The relationship between Lab. culture toxicity and Toxkits toxicity indicated good relation ($r^2=0.84$, p<0.01, n = 83). Number of samples which showed a small difference of lower than 0.5 between two test methods were 52 and they account for 63 percent of collected samples. In addition, these 52 samples had a lower average toxicity of TU 0.5 (Lab. culture method) and TU 0.45 (Toxkits method). Whereas samples which indicated big difference of test results between two methods, had a tendency to show higher toxicity. From these results, alternative toxicity test method could be applied to the official test method, if samples would have a lower toxicity less than TU 2. Also, Toxikit standard toxicant test results indicated $EC_{50}$ values between 0.93 and 1.68 mg/L and these results were considered as valid for quality control standard.

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References

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