• 한국축산식품학회지
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• 제37권5호
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• pp.654-662
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• 2017

Extracellular microvesicles are membranous nano-sized cellular organelles secreted by a variety of cells under normal and pathological conditions and heterogeneous in size ranging from 30 nm to $1{\mu}m$. They carry functional microRNAs that can influence immunity and development. For a particular application of microvesicles, choice of isolation method is particularly important; however, their isolation methods from colostrum in particular have not been described clearly. In this work, differential ultracentrifugation as a conventional method, ultracentrifugation with some modification such as additional precipitations, ultrafiltration, sucrose gradient separation and ExoQuick$^{TM}$ as a commercial reagent were compared. The goal was to compare mainly microvesicular total microRNA yield, distribution and purity among the methods then select the best isolation method for bovine colostrum microvesicles based largely on microRNA yield with the view of applying the vesicles in work where vesicular microRNA cargo is the target bioactive component. Highest yields for vesicular microRNA were obtained using conventional methods and among them, subsequent ultracentrifugation with 100,000 g and 135,000 g conventional method 2 was selected as it had the highest RNA to protein ratio indicating that it pelleted the least protein in relation to RNA an important factor for in vivo applications to assess microvesicle functionalities without risk of contaminating non-vesicular biomaterial. Microvesicles isolated using conventional method 2 were successfully internalized by cells in vitro showing their potential to deliver their cargo into cells in vitro and in vivo in case of functional studies.

• 한국미생물·생명공학회지
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• 제20권6호
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• pp.655-662
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• 1992

알칼리내성 Bacillus sp. Ya-14 유래의 pectate lyase 유전자를 함유한 재조합균주로부터 affinity method, CM-cellose column chromatography와 gel filtration을 통해 효소를 정제하였으며 정제효소의 수율은 10.2, 정제도는 258배였다. 효소의 최적활성 pH는 10.0이었고 pH4.0-10.0까지의 범위에서 안정성이 있었으며, 최적활성온도는 $60^{\circ}C$이고 $50^{\circ}C$까지 열안정성이 있으며, SDS-PASGE에 의해 추정된 분자량은 43KDa 이었다. 아미노산 조정 분석 결과 polar, basic 아미노산의 함량이 높고 특히 Ser, Gly, Tyr의 함량이 높았으며, 정제효소의 N-terminal은 Ala-Asp-Leu-Gly-His-Gln-Thr의 아미노산 서열이었다.

• 한국식품조리과학회지
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• 제21권5호
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• pp.662-668
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• 2005

To investigate the quality retention method of salted and semi-dried mackerel fillets, pH, volatile basic nitrogen (VBN), trimethylamine (TMA) and total bacterial counts in the fillets that were UV treated for 1, 3, and 4 hr were periodically measured during 48 days of refrigerated storage. The moisture, crude protein, crude lipid, and ash contents were 45.46 %, 18.87 %, 33.75 %, and 2.99 %, respectively. The freshness as determined by pH measurement could be maintained up to 25 days for the control and 31 days for the UV-treated fillets. VBN contents were continually increased over the storage time. The control reached the initial putrid level after 21 days while the UV-treated fillets after $25{\sim}31$ days. A similar tendency was shown for the changes of trimethylamine TMA contents, although the period differed slightly and the increase of the UV treatment time could decrease the TMA contents. The changes on the total bacterial counts of the fillets were maintained under 105 CFU/g during 48days. Especially, due to the effective decrease of the microorganism count by UV treatment at the beginning of the storage, UV -treated fillets showed lower bacterial counts than control over the whole period. E. coli. and Listeria were not detected in any fillets. Combining the above data and the sensory changes, the storage period of the fillets could be prolonged from 15 days at present to 30 days by UV treatments.

• 동아시아식생활학회지
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• 제15권6호
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• pp.655-662
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• 2005

Body content bone mined density and nutrient intake status of 129 college women in Daegu area are analyze4 The mean age was 23.1 years. Bone mineral density was measured in their right heel by SONOST-2000 ultrasound bone densitometer, and nutrient intake was assessed by a Questionnaire. Diet intake data were obtained by using a 24 hours recall method to evaluate the subject's usual diet. The average energy, Ca, Ee, Zn and folate intakes were 64.2, 47.5, 54.7, 62.9 and $57.4\%$ of RDA, respectively. Ca/P and Ca/Protein ratio were 0.46 and 6.7, respectively. The mean adequacy ratio(MAR), an index of overall dietary quality wag 0.6. The index of nutritional quality was under 1.0 for protein(0.7), vitamin A(0.9), E(0.7), $B_1(0.8)$, niacin(0.8), $B_6(0.6)$ and phosphorus(0.6). The less the protein and energy intake, the less the bone mineral density(p<0.05). The intakes of protein(p<0.05) and that of phosphorus(p<0.001) were positively correlated with bone quality index(BQI). The calcium intake was positively correlated with Z-score(p<0.05). The phosphorus intake was positively correlated with T-score(p<0.001) and Z-score(p<0.0001). In conclusion, this study indicates that nutrient intake of the college women is considerably lower than the RDA. Also the BQI is lower than standard.

• Molecules and Cells
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• 제43권7호
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• pp.662-670
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• 2020

We have investigated the involvement of the pre-mRNA processing factor 4B (PRP4) kinase domain in mediating drug resistance. HCT116 cells were treated with curcumin, and apoptosis was assessed based on flow cytometry and the generation of reactive oxygen species (ROS). Cells were then transfected with PRP4 or pre-mRNA-processing-splicing factor 8 (PRP8), and drug resistance was analyzed both in vitro and in vivo. Furthermore, we deleted the kinase domain in PRP4 using Gateway™ technology. Curcumin induced cell death through the production of ROS and decreased the activation of survival signals, but PRP4 overexpression reversed the curcumin-induced oxidative stress and apoptosis. PRP8 failed to reverse the curcumin-induced apoptosis in the HCT116 colon cancer cell line. In xenograft mouse model experiments, curcumin effectively reduced tumour size whereas PRP4 conferred resistance to curcumin, which was evident from increasing tumour size, while PRP8 failed to regulate the curcumin action. PRP4 overexpression altered the morphology, rearranged the actin cytoskeleton, triggered epithelial-mesenchymal transition (EMT), and decreased the invasiveness of HCT116 cells. The loss of E-cadherin, a hallmark of EMT, was observed in HCT116 cells overexpressing PRP4. Moreover, we observed that the EMT-inducing potential of PRP4 was aborted after the deletion of its kinase domain. Collectively, our investigations suggest that the PRP4 kinase domain is responsible for promoting drug resistance to curcumin by inducing EMT. Further evaluation of PRP4-induced inhibition of cell death and PRP4 kinase domain interactions with various other proteins might lead to the development of novel approaches for overcoming drug resistance in patients with colon cancer.

• Nuclear Engineering and Technology
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• 제55권9호
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• pp.3268-3276
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• 2023

The practice of identifying the potential zones for mineral exploration in a speedy and low-cost method includes the use of satellite imagery analysis as a part of remote sensing techniques. It is challenging to explore the iron mineralization of a region through conventional methods which are a time-consuming process. The current study utilizes the Hyperion satellite imagery for mapping the iron mineralization and associated geological features in the Irikkur region, Kannur, Kerala. Along with the remote sensing results, the field study and laboratory-based analysis were conducted to retrieve the ground truth point and geochemical proportion to verify the iron ore mineralization. The MC simulation showed for shielding properties indicate an increase in the linear attenuation coefficient with raising the Fe₂O₃+SiO₂ concentrations in the investigated rocks where it is varied at 0.662 MeV in the range 0.190 cm^-1 - 0.222 cm^-1 with rising the Fe₂O₃+SiO₂ content from 57.86 wt% to 71.15 wt%. The analysis also revealed that when the γ-ray energy increased from 0.221 MeV to 2.506 MeV, sample 1 had the largest linear attenuation coefficient, ranging from 9.33 cm1 to 0.12 cm^-1. Charnockite rocks were found to have exceptional shielding qualities, making them an excellent natural choice for radiation shielding applications.

• Nuclear Engineering and Technology
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• 제55권7호
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• pp.2380-2387
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• 2023

Hyperspectral data and its ability to explore the minerals and their associated rocks have a remarkable application in mineral exploration and lithological characterization. The present study aims to explore the radiation shielding aspects of the iron ore in Kerala with the aid of the Hyperion hyperspectral dataset. The reflectance-spectra obtained from the laboratory conditions as well as from the image show various absorptions. The results from the spectra are validated with geochemical data and GPS points. The Monte Carlo simulation employed to evaluate the radiation shielding ability. Raising the oxygen ions caused a noteworthy decrease in the µ values of the studied rocks which is accompanied by an increase in Δ_0.5 and Δ_eq values. The Δ_0.5 and Δ_eq values increased by factors of approximately 77 % with raising the oxygen ions between 44.32 and 47.57 wt.%. The µ values varies with the oxygen concentrations, where the µ values decreased from 2.531 to 0.925 cm^-1 (at 0.059 MeV), from 0.381to 0.215 cm^-1 (at 0.662 MeV), and from 0.279 to 0.158 cm^-1 (at 1.25 MeV) with raising the oxygen ions from 44.32 to 47.43 wt.%.

• 문화기술의 융합
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• 제9권6호
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• pp.479-486
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• 2023

본 연구는 간호대학생의 MBTI 선호지표에 따른 갈등관리유형의 차이를 확인하기 위한 서술적 조사연구이다 자료수집은 2023년 8월 30일부터 9월 30일까지였다. 자료분석은 independent t-test, One-way ANOVA, Scheffe's를 시행하였다. 연구결과 갈등관리유형에 유의미한 차이를 보이는 MBTI지표 중 에너지 방향에서 I는 E보다 회피가 높았다(t=-3.776, p<0.01). 의사결정기능의 경우 F가 T보다 양보(t=-3.478, p<0.01)와 회피(t=-3.389, p<0.01)가 높았고, T가 F보다 지배가 높았다(t=2.070, p<0.5). 외부생활 대처양식의 경우 J가 P보다 협력(t=2.756, p<0.01)과 타협(t=2.044, p<0.5)이 높았다. MBTI의 심리기능유형에서는 NF형이 ST형보다 양보가 높았고(F=4.174, p<0.05), SF형이 ST형보다 회피가 높았다(F=4.202, p<0.05). MBTI 의사결정기능 유형과 외부생활 대처양식을 조합해서 갈등관리유형의 차이를 분석한 결과는 FJ형이 FP형 보다 협력이(F=2.907, p<0.05), FJ형이 TP형보다 양보가(F=4.662, p<0.01), FJ형이 TJ형보다 회피가 높았다(F=3.327, p<0.05). MBTI의 태도지표는 EJ형이 EP형 보다 협력이 높았고(F=2.817, p<0.05), IP형이 EP형 보다 회피가 유의미하게 높았다(F=4.551, p<0.01). 본 연구에서는 현재까지 국내에서는 연구되지 않은 MBTI 의사결정기능 유형(F,T)과 외부생활대처양식(J,P)을 조합해서 갈등관리유형의 차이를 확인한 것에 의의가 있다. 후속연구에서는 본 연구결과를 반영하여 대상자 수를 확대한 MBTI 성격유형별 갈등관리유형에 대한 연구, MBTI 지표 및 성격유형별 갈등관리 프로그램개발과 프로그램 효과성 분석 연구를 제언한다.

• 한국버섯학회지
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• 제12권2호
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• pp.127-131
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• 2014

큰느타리버섯의 품종다양화와 보급확대를 목적으로 발생개체수가 적고 생력재배에 적합한 "새곤지"의 특성을 조사한 결과는 다음과 같다. 균사생장을 위한 적정온도범위는 $23{\sim}26^{\circ}C$로 대조품종 $26{\sim}29^{\circ}C$보다 낮았다. 자실체 발생 및 생육적온은 $15{\pm}1^{\circ}C$였으며, 재배기간은 51일로 대조품종과 동일하였다. 갓형태는 생육초기 반반구형에서 생육후기까지 평반구형의 갓형태를 유지하였다. 병당 발생개체수는 3.5개로, 대조품종 12.8개 대비 9.3개 적었다. 대길이는 135.8 mm로 대조품종 124.6 mm보다 길었고, 갓표면의 색도(Lightness)는 60.4로 대조품종 57.6보다 높아 연회색을 나타내었다. 탄력성과 응집성은 대등하였고, 깨짐성, 경도값은 각각 71,068 g, $5,662g/cm^2$으로 대조품종보다 낮았다. 병당수량은 900 cc/$65{\Phi}$ 병에서 106.4 g, 1100 cc/$75{\Phi}$ 병에서159 g으로 대조품종 과 대등하였다.

• 한국산림과학회지
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• 제95권6호
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• pp.657-662
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• 2006

본 연구는 전남 완도수목원 내 붉가시나무 임분을 대상으로 간벌이 토양의 화학적 특성 변화에 미치는 영향을 조사하기 위하여 실시하였다. 간벌은 흉고 직경급 10 cm일 때의 상수리나무 잔존 본수를 기준으로 실시하였으며, 토양 시료는 간벌 후 7년이 경과한 이후 채취하였다. 간벌 후 토양 pH는 대조구와의 차이가 나타나지 않았으나, 토양 유기물, 전질소 및 유효인산 농도는 간벌 처리구가 대조구에 비해 높았다. 간벌 후 토양 내 양이온치환 용량(C.E.C)과 치환성 양이온($K^+$, $Na^+$, $Ca^{2+}$, $Mg^{2+}$) 농도 역시 증가하였다. 붉가시나무 임분 내 토양의 양분 농도를 조사한 결과 일반 산림 토양에 비해 낮은 것으로 조사되어, 적절한 간벌 등을 통한 난대림 임분 개선과 갱신을 위한 시업방안 개발이 보다 체계적으로 마련되어야 한다고 판단된다.