• Title/Summary/Keyword: E5a

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Purification and Characterization of a Major Fibrinolytic Enzyme from Bacillus amyloliquefaciens MJ5-41 Isolated from Meju

  • Jo, Hyeon-Deok;Lee, Hwang-A;Jeong, Seon-Ju;Kim, Jeong-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.21 no.11
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    • pp.1166-1173
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    • 2011
  • Meju is a traditional Korean fermented soy product used as a key element for soy sauce and doenjang. Bacilli with antimicrobial activity were isolated from meju prepared by traditional methods at Sunchang county, Jeollabukdo, Korea. Six isolates were identified as Bacillus amyloliquefaciens by recA gene sequencing and RAPD-PCR. One isolate, B. amyloliquefaciens MJ5-41, showed the strongest fibrinolytic activity. A 27 kDa active fibrinolytic enzyme, AprE5-41, was purified from the culture supernatant of MJ5-41 grown on LB by chromatographic methods. The optimum pH and temperature for purified AprE5-41 were 7.0 and $45^{\circ}C$, respectively. AprE5-41 quickly degraded $A{\alpha}$ and $B{\beta}$ chains but not the ${\gamma}$-chain of fibrinogen. AprE5-41 exhibited the highest specificity for N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide, a known substrate for ${\alpha}$-chymotrypsin, cathepsin G, and subtilisin BPN'. The structural gene, aprE5-41, was cloned by PCR and successfully expressed in B. subtilis.

The Development of Post-Processing GPS(L1)/Galileo(E1/E5a) Software Receiving Platform using MATLAB (GPS(L1)/Galileo(E1/E5a) 다중 신호 통합 수신 소프트웨어 플랫폼 개발)

  • Jeon, Sang-Hoon;So, Hyoung-Min;Lee, Taek-Jin;Kim, Ghang-Ho;Jeon, Seung-Il;Kim, Jong-Won;Kee, Chang-Don;Cho, Young-Su;Choi, Wan-Sik;Lee, Sang-Uk;Kim, Jae-Hoon
    • Journal of Advanced Navigation Technology
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    • v.13 no.3
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    • pp.311-318
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    • 2009
  • This paper shows the research about the development of software receiving platform processing GPS/Galileo L1/E1/E5a signal. Various researches for new GNSS signal character are possible using software receiving platform by facile program code modification. In addition, the program that processes GPS and Galileo signal integration is expected to help developing integration of receiver algorithm that deal with new various GNSS signal. In this paper, it is introduced the structure of GPS/Galileo receiving platform using sampled IF data as a program input. The function of the software platform embodied using MATLAB tool is tested by live data from Galileo test satellites. The software platform is modulated according to their roll and function. Each module is able to use selective function on GNSS signal.

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The Relationship among Web Design, e-Satisfaction, e-Loyalty, and Customer Defection (웹디자인, e-만족, e-충성도, 고객이탈 간의 관계에 관한 연구)

  • Kim, Keum Soo;Chung, Ki Han
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.5 no.4
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    • pp.213-229
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    • 2009
  • The purpose of this paper is to identify the relationship among five factors of web design, e-satisfaction, e-loyalty, and customer defection and provide the implications of marketing strategies in online shopping. The research findings are as follows: first, ease of use of web design affects positively e-satisfaction more than the other factors except financial security. Second, e-satisfaction affects positively e-loyalty. Third, e-satisfaction does not affect customer defection. Fourth, informativeness, styling, ease of use, and trust of web design affect indirectly e-loyalty by mediating e-satisfaction. Therefore, because there is a good circular relationship among web design, e-satisfaction, and e-loyalty, it is very important to enhance web design for customers. When customers satisfy highly with the factors of web design, e-loyalty is higher and customer defection is lower. Further, in a curriculum of web design it is needed to reflect the findings of this paper.

A study on the Dielectric Properties of IPN based on the Epoxy/Silicon and Epoxy/Urethane (Epoxy/Silicon Epoxy/Urethane계 IPN 복합재료의 개발에 관한 연구)

  • Shin, Joong-Hong;Jung, Eun-Shik;Park, Chung-Hoo
    • Proceedings of the KIEE Conference
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    • 1987.11a
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    • pp.504-507
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    • 1987
  • Interpenerating Polymer Networks (IPNs) are unique type of polymer blend, synthesized by swelling a crossed polymer (Epoxy) with second polymer (Silicon) and also we adopted Urethane as the second polymer. The relationship between dielectric and mechanical properties of high temperature curing IPNs(E/S, E/U) are investigated. The ratios of weight that we formed we re two kind of thing, one (E/S) about 1[wt%]. 3[wt%], 5[wt%], 7[wt%], 10[wt%], and the other (E/U) about 5[wt%], 15[wt%], 25[wt%]. It was heat-cured for 24hours at $100^{\circ}C$ 48 hours at $10^{\circ}C$, 5 hours at $130^{\circ}C$, 15hours at $130^{\circ}C$ in E/S and also for 5 hours at $130^{\circ}C$ in E/U. From the viewpoint of dielectric and mechanical properties, the optimum condition is obtained from the sample cured for 5hours at $130^{\circ}C$ for 1[wt%] in the E/S, and also obtained from the sample cure d for 5 hours at $130^{\circ}C$ in E/U.

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An Investigation of Characteristics of Chinese Bellflower (Platycodon grandiflorum A.) Cultivated Soil

  • Choi, Jang Nam;Lee, Wang Hyu
    • Korean Journal of Plant Resources
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    • v.31 no.6
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    • pp.660-666
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    • 2018
  • In order to understand the characteristics of soil according to the cultivation environment of Chinese bellflower (Platycodon grandiflorum A.), soil chemical properties of 12 collected soil samples from 6 cultivated fields in Okcheon, Chungbuk province in August. 2017 were analyzed. The soil pH was distributed within the range of 4.61 to 5.25 at all cultivation years and E.C (Electric Conductivity) and T-N (Total Nitrogen) of the cultivation year were not significant. Available $P_2O_5$ was higher than the average for medicinal crops and P. grandiflorum in Korea and C.E.C (Cation Exchange Capacity) was inconsistent for each cultivation year. In particularly, it was validated that the content of exchangeable cations K, Ca, Ma, and Na in this experiment was similar to that of C.E.C according to the cultivation years, because C.E.C had a high correlation with the exchangeable cations. For the available $P_2O_5$, as affected by trans-planting, 5Y-NT-H (cultivated 5 years and non-transplanted) had 58 mg/kg, while 5Y-T-H (cultivated 5 years and transplanted) had 246 mg/kg. The soil pH was found to be lower (acidic) in diseased soils than healthy soils. E.C was confirmed to be was higher in diseased soils than healthy soils except for the one cultivated for 2 years. The contents of T-N and available $P_2O_5$ were higher in diseased soil except for the one cultivated for 5 years and 11 years. The exchangeable cation K and Na tended to be higher in diseased soils rather than that in healthy soils, and the exchangeable cation Ca and Mg contents were higher in healthy soils than in diseased soils. The C.E.C of the soil was lower than that of healthy soils in all of the years except for the one which was cultivated for 5 years (transplanted).

Production of Saccharogenic and Dextrinogenic Amylases by Rhizomucor pusillus A 13.36

  • Silva Tony M.;Attili-Angelis Derlene;Carvalho Ana Flavia Azevedo;Silva Roberto Da;Boscolo Mauricio;Gomes Eleni
    • Journal of Microbiology
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    • v.43 no.6
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    • pp.561-568
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    • 2005
  • A newly-isolated thermophilic strain of the zygomycete fungus Rhizomucor pusillus 13.36 produced highly active dextrinogenic and saccharogenic enzymes. Cassava pulp was a good alternative substrate for amylase production. Dextrinogenic and saccharogenic amylases exhibited optimum activities at a pH of 4.0-4.5 and 5.0 respectively and at a temperature of $75^{\circ}C$. The enzymes were highly thermostable, with no detectable loss of saccharogenic or dextrinogenic activity after 1 hand 6 h at $60^{\circ}C$, respectively. The saccharogenic activity was inhibited by $Ca^{2+}$ while the dextrinogenic was indifferent to this ion. Both activities were inhibited by $Fe^{2+}\;and\;Cu^{2+}$ Hydrolysis of soluble starch by the crude enzyme yielded $66\%$ glucose, $19.5\%$ maltose, $7.7\%$ maltotriose and $6.6\%$ oligosaccharides.

Effects of Dietary Vitamin E Level and Caffeine on Lipid Peroxidation in Rat Liver (흰쥐 간에 있어 지질과산화에 미치는 식이성 비타민 E의 수준 및 카페인의 영향)

  • 박미리;조수열
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.23 no.4
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    • pp.561-567
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    • 1994
  • This study was designed to evaluate the effects of dietary vitamin E and caffeine on the activities of lipid peroxidation related enzymes in rat liver . Male Sprague-Dawley rats were fed on diets containing three level of vitamin E (37.5, 750 or 1,5oomg/kg diet) and with or without 0.3% caffeine. The rats were sacrificed after 5 and 10 weeks of feeding. Results obtained from this study were as follows ; The content of cytochrome P450 tended to increase as dietary vitamin E level was raised. The activity of xanthine oxidase increased in the caffeine groups, but it decreased by the increasing level of vitamin E. Superoxide dismutase and catalase activity were slightly elevated by dietary supplementation of vitamin E. And there was a tendency of higher these enzyme activity of caffeine groups. The activity of glutathione perxidase tended to decrease as dietary vitamin E level increased. But it was raised by caffeine supplementation . Liver glutathione content was not affected by dietary supplementation of vitamin E, but it showed a decreasing tendency in caffeine groups. There was a tendency of more lipid peroxide content of caffeine groups than that of the only vitamin E supplemented group. But the degree of increment of this decreased as dietary vitamin E level increased.

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Ecklonia cava-Hizikia fusiformis complex extract alleviates inflammation in human lung epithelia

  • Lee, Sung-Gyu;Kwon, Sang-Oh
    • Journal of Plant Biotechnology
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    • v.49 no.1
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    • pp.90-98
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    • 2022
  • This study was performed to determine the optimal ratio for preparing an extract comprising the Ecklonia cava and Hizikia fusiformis complex as a therapeutic material for alleviating inflammatory respiratory diseases. First, to examine the optimal ratio for preparing the complex (SD-EH), Ecklonia cava and Hizikia fusiformis extracts were used; four extracts with different mixing ratios were prepared. The effects of the SD-EH extract on MUC5AC mRNA expression in PMA-treated NCI-H292 cells were analyzed; it was confirmed that the MUC5AC expression was significantly reduced after treatment with the SD-EHA-001 (E(100) : H(0)), SD-EHB-001 (E(90) : H(10)), SD-EHC-001 (E(80) : H(20)), and SD-EHD-001 (E(70) : H(30)) extracts. Western blotting was used to determine whether the SD-EH extract affects the expression levels of COX-2 and MMP-9 in PMA-treated A549 cells. The protein expression levels of COX-2 and MMP-9 were significantly lower (p < 0.001) in the cells treated with the SD-EHC-001 (E(80):H(20)), SD-EHD-001 (E(70) : H(30)), and SD-EHE-001 (E(60) : H(40) extracts than in the cells treated with PMA alone. The SD-EHC-001 (E(80) : H(20)) extract markedly downregulated the expression levels of MUC5AC, COX-2, and MMP-9. Therefore, the SE-EH extract may serve as a potential therapeutic agent for treating inflammatory respiratory diseases.

Effect of 5-Hydroxytryptamine(5-HT) on Renal Function in Dog (5-Hydroxytryptamine(5-HT)이 개의 신장기능에 미치는 영향)

  • Ko, Suk-Tai;Na, Han-Kwang;Choe, In
    • Biomolecules & Therapeutics
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    • v.4 no.1
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    • pp.7-18
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    • 1996
  • 5-Hydroxytryptamine(5-HT, serotonin), when given into the vein, produced antidiuretic action accompanied with reduction of glomerular filtration(GFR), renal plasma flow(RPF), osmolar clearance(Cosm) and amounts of sodium or potassium excreted in urine( $E_{Na}$ , $E_{K}$), with the augmented reabsorption rates of sodium and potassium in renal tubules. 5-HT, when infused into a renal artery, exhibited diuretic action accompanied with the augmented RPF and increased $E_{Na}$ and $E_{K}$ in only infused kidney. Antidiuretic action of 5-HT infused into the vein was not influenced by ketanserin, 5-H $T_2$receptor blockade, given into a renal artery, vein or carotid artery, by methysergide, 5-H $T_1$receptor blockade, given into a renal artery, whereas above antidiuretic action was inhibited by methysergide given into vein or carotid artery. Diuretic action of 5-HT infused into a renal artery in only experimental kidney was blocked by ketanserin injected into a renal artery, was not influenced by methysergide administered into a renal artery. Above results suggest that 5-hydroxytryptamine(5-HT) produced the antidiuretic action through central 5-H $T_1$receptor and the diuretic action through 5-H $T_2$receptor located in renal tubules of kidney.ney.

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Digital persona in E-girl and E-boy fashion images (E-girl과 E-boy 패션 이미지에 나타난 디지털 페르소나)

  • Uh, Kyung Jin
    • The Research Journal of the Costume Culture
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    • v.28 no.5
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    • pp.692-704
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    • 2020
  • The aim of this study is to select MLMA and Noen Eubanks as representatives of Gen Z's unique subculture 'E-girl' and 'E-boy' to analyze fashion images and determine their meaning through the perspective of persona. To conduct this study, a literature review and case studies were combined and fashion images of E-girl and E-boy appearing on social media were analyzed to identify the personas they expressed. A case study of MLMA and Noen Eubanks' Instagram and TikTok account posts showed that grotesque, kitsch, and gender-related images stand out. The digital personae demonstrated by E-girl and E-boy fashion images were demonstrated to be a strange persona that reject perfect beauty and a playful persona escaping from reality and anxiety. Lastly persona of individual identity was shown to be formed. This study provides a basis for understanding and interpreting Gen Z's culture and aesthetics that will lead to future trends. Based on this study, I hope that various studies on E-girl and E-boy apparel will be conducted, and that by understanding the importance of E-girl and E-boy culture for Gen Z (which is paying attention to fashion trends), this can be used as a marketing strategy to consider their characteristics, with data used to inform design development.